Trichoderma viride fungi and preparation and application of fungicide thereof

A technology of Trichoderma viridans and fungi, applied in the direction of application, fungicides, fungi, etc., to achieve the effect of broad antibacterial spectrum, significant control effect, and good antibacterial effect

Active Publication Date: 2011-05-25
HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, studies on the use of Trichoderma to control sheath blight have also been frequently reported

Method used

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  • Trichoderma viride fungi and preparation and application of fungicide thereof
  • Trichoderma viride fungi and preparation and application of fungicide thereof
  • Trichoderma viride fungi and preparation and application of fungicide thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Embodiment 1: the isolation and screening of Trichoderma viride ZBS6 bacterial strain

[0022] (1) Separation and Purification of Trichoderma The collected soil sample was dried in the shade and ground, weighed 5 g, and put it into a 150 ml Erlenmeyer flask filled with 45 ml of normal saline. Using the soil dilution separation method, draw 1mL of the diluent and add it to a test tube containing 9mL of water, shake and mix to prepare 10 -2 Soil dilution. Take 0.1ml of the soil dilution and spread it on the PDA medium containing sodium propionate and streptomycin sulfate, and incubate it upside down in a constant temperature incubator at 25°C for 3 days. The isolated Trichoderma colonies were picked, separated and purified from single spores, and then transferred to PDA slant medium. After the mycelia and spores covered the slant, sterilized paraffin oil was added for storage for later use.

[0023] (2) The tested pathogens include Fusarium graminearum, Fusarium oxyspor...

Embodiment 2

[0031] Embodiment 2: Identification of ZBS6 bacterial strain

[0032] (1) Morphological classification and identification Trichoderma was identified by colony morphology observation and microscopic observation, and its species was determined by searching literature.

[0033] (2) Molecular identification Antagonistic Trichoderma genomic DNA was extracted with reference to the DNA extraction method of Wu Fahong and other fungi, and extracted with ddH 2 O dissolved DNA to 500ng / μl. The upstream and downstream primers used to amplify the ITS sequence of Trichoderma were synthesized by Shanghai Sangon Bioengineering Technology Service Co., Ltd., respectively ITS4: 5'-GGAAGTAAAAGTCGTAACAAGG-3' and ITS6: 5'-TCCTCCGCTTATTGATATGC-3'. Using the genomic DNA of the antagonistic Trichoderma as a template, PCR amplification was performed using primers ITS4 and ITS6. The PCR reaction system is 25μl: 10×PCR Buffer 2.5μl, template 1μl, dNTP (10mM each) 0.5μl, primer ITS4 (10mM) 1μl, ITS6 (10...

Embodiment 3

[0045] Embodiment 3: the preparation of a kind of microbial bacterial agent

[0046] (1) Preparation of liquid bacterial agent Transfer the preserved Trichoderma viride strain ZBS6 onto a PDA medium plate, culture in alternating light and dark at 25°C for 4 days, scrape conidia under aseptic conditions, and prepare 1 × 10 with sterile water. 7 The conidia suspension was inoculated according to 1ml spore suspension / 100ml PDB medium, placed at 25°C and shaken at 150rpm for 3 days, stirred with an electric stirrer at 2000rpm for 2min, and mixed with 1.9L phosphate buffer (inner Containing 0.1% auxiliary agent Tween 100 and 0.2% preservative benzoic acid), the liquid biocontrol agent can be obtained.

[0047] (2) Preparation of solid bacterial agent Transfer the preserved Trichoderma viride strain ZBS6 onto a PDA medium plate, culture in alternating light and dark at 25°C for 4 days, scrape conidia under aseptic conditions, and prepare 1×10 with sterile water. 7 The conidia suspe...

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Abstract

The invention discloses a trichoderma viride fungi strain and a method for preparing a bio-control fungicide thereof and application. The strain is named as trichoderma viride strain ZBS6 and collected in the CGMCC, and the collection number is CGMCC No.3538. The microbial fungicide is liquid or solid fungicide, and is prepared from the trichoderma viride ZBS6 strain. The ZBS6 strain and the fungicide thereof have wide bacteriostatic spectrum and good bacteriostatic effect, and have strong inhibiting effect on botrytis cirerea, rhizoctonia solani, rhizocto-nia cerealis, gaeumannomyces grami, marssonina coronaria, fusarium oxysporum and the like; and the fungicide can be used for control of botrytis cirerea, cucumber downy mildew, rhizocto-nia cerealis, wheat soil-borne disease and wheat powdery mildew, is efficient, non-toxic and simple to use, and does not cause environmental pollution.

Description

technical field [0001] The invention relates to the field of biological control of plant diseases, in particular to a Trichoderma viride strain capable of preventing and treating various plant diseases, a method for preparing a biocontrol agent by using the strain, and an application of the biocontrol agent. technical background [0002] Plant diseases are the great enemy of agricultural production. According to the statistics of the Food and Agriculture Organization of the United Nations (FAO), the annual average loss of production reduction caused by plant diseases is 10% to 15% of the total output. For centuries, chemical pesticides have played an active role in the prevention and control of plant diseases, insect pests and weeds, and have made important contributions to the stability, increase and loss recovery of agriculture. However, excessive application and abuse of pesticides have led to many negative effects, such as soil fertility decline, environmental pollution,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
Inventor 孙虎薛保国杨丽荣全鑫武超刘明源姚刚林
Owner HENAN ACAD OF AGRI SCI
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