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Endophytic antagonistic strain for controlling soft rot of camellia oleifera and application thereof

A technology of soft rot and bacterial strains, which is applied to Bacillus amyloliquefaciens L46, which is highly effective in preventing and controlling soft rot of camellia oleifera, and its application fields, can solve the problems of weak or even blank technology, and no biological pesticides for preventing and treating soft rot of camellia oleifera, and achieve good development The effect of application prospect, easy industrial production, and simple cultivation conditions

Active Publication Date: 2011-09-07
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0005] Isolation and screening of Camellia oleifera plants to obtain biocontrol strains with antagonistic effects on Camellia oleifera soft rot is of great significance to the prevention and treatment of Camellia oleifera soft rot, but the relevant technology is still weak or even blank, and it has been able to effectively control Camellia oleifera soft rot for more than 20 years. Antagonistic bacteria against rot disease have not been reported, and there are no reports of biological pesticides for preventing and treating soft rot of camellia oleifera

Method used

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  • Endophytic antagonistic strain for controlling soft rot of camellia oleifera and application thereof
  • Endophytic antagonistic strain for controlling soft rot of camellia oleifera and application thereof
  • Endophytic antagonistic strain for controlling soft rot of camellia oleifera and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The separation of embodiment 1 bacillus amyloliquefaciens L46

[0027] The healthy leaves of common camellia oleifera are taken, separated, purified and preserved by conventional methods.

[0028] After the camellia oleifera was rinsed with water, an appropriate amount of healthy tissue was selected, weighed separately, cut into 5mm×5mm tissue pieces, soaked in 75% ethanol for 1min, then soaked in 0.1% mercuric solution for 40s-70s, and then placed Soak in 75% ethanol for 30s, take it out and rinse it with sterile water 4 times. Add 10ml of sterile water and a little sterilized quartz sand into a sterile mortar to grind into a homogenate, cover and let stand for 30 minutes, serially dilute the homogenate 10 times, and pipette 0.1ml to prepare After smearing evenly on a good NA plate, put it into an incubator for cultivation, record the amount of bacteria after 48 hours, and select according to the shape, color and size of the colony, purify and store on the plate, and ...

Embodiment 2

[0029] The screening of embodiment 2 bacillus amyloliquefaciens L46

[0030] Preliminary screening: use the confrontation culture method. Screening of endophyte antagonistic pathogenic fungi. Put the 6mm-diameter oleiferia soft rot bacterial cake in the center of the plate, and then use the inoculation ring to pick up the suspension of the bacteria to be tested and pick up 2 to 3 kinds of endophytic bacteria equidistantly (3cm from the center of the plate). Incubate in the dark at 25°C in an incubator, observe and record the presence and size of the bacteriostatic zone after 4 days, and repeat 3 times in total. Select 10 strains with a bacteriostatic zone width greater than or equal to 4mm for re-screening, followed by L1, L6, L12, L19, L29, L33, L35, L39, L46, L48, and re-screen (see Table 1).

[0031] Table 1 Antibacterial effect of endophytic bacteria on Camellia oleifera soft rot

[0032]

[0033] Re-screening: use fermentation method. After the 10 initially screene...

Embodiment 3

[0036] Example 3 The inhibitory effect of Bacillus amyloliquefaciens L46 on the hyphae of camellia oleifera soft rot

[0037] Put the Camellia oleifera soft rot bacterial cake with a diameter of 6mm in the center of the PDA plate of the potato sucrose medium, and then inoculate the antagonistic bacteria L46 at an equal distance of 3cm, repeat three times, culture at 25-28°C, and measure the diameter of the inhibition zone after 4 days. Depend on image 3 It can be seen that Bacillus amyloin L46 has a strong antibacterial effect on Camellia oleifera soft rot.

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Abstract

The invention discloses an endophytic antagonistic strain for controlling soft rot of camellia oleifera and application thereof. The strain is bacillus amyloliquefaciens sp. L46, with a collection number of CCTCC M2010324. The bacillus amyloliquefaciens L46 is obtained by flat-plate antagonistic test sieving, can be used for effectively suppressing growth of soft rot pathogenic bacteria of camellia oleifera, and has the advantages of high prevention effect, high environmental safety and broad development and application prospect.

Description

technical field [0001] The invention belongs to the field of plant protection, and in particular relates to an endogenous antagonistic strain for preventing and treating soft rot of camellia oleifera and its application, in particular to a bacillus amyloliquefaciens L46 for effectively preventing and treating soft rot of camellia oleifera and its application. Background technique [0002] Camellia oleifera (Camellia oleifera) is a unique edible oil tree species in my country and one of the four largest woody oil tree species in the world. It has a history of utilization and cultivation for more than 2,000 years. It has wide adaptability and strong stress resistance, and is suitable for planting in vast red soil hilly low areas. It mainly grows in the south, mainly in Hunan, Jiangxi, and Guangxi. Among them, Hunan Province is the largest camellia oleifera producing area and the largest camellia oil consumption area in China. [0003] At present, there are many kinds of disea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/07
Inventor 刘君昂李雪娇周国英李琳宋光桃
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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