301 results about "Soft rot disease" patented technology

The invention provides bacillus amyloliquefaciens K-8 and bactericide thereof. The preservation number of the bacillus amyloliquefaciens K-8 strain is CGMCCNO.6486. The bactericide takes the bacillus amyloliquefaciens K-8 strain as an active ingredient. The bacillus amyloliquefaciens K-8 strain is cultured to obtain a liquid bactericide, and a solid bactericide can be obtained by adding a carrier or mixture of the carrier and auxiliaries into the liquid bactericide. The bacillus amyloliquefaciens K-8 strain and the bactericide thereof provided by the invention can be used for effectively preventing and treating diseases such as bacterial wilt, wilt, angular spot, banded sclerotial blight, rice false smut, soft rot, rot, canker and root rot. The bactericide is simple to prepare and easy to use, is applicable to industrialized production and has wide application prospect.

The invention relates to the field of microbial control, in particular to bacillusamyloliquefaciens subsp Lh-1 and application thereof. With a preservation number of CGMCC No.8548, the bacillusamyloliquefaciens subsp Lh-1 is applied in control of Verticillium dahliae, erwinia carotovora, ralstonia solanacearum, valsa leucostoma, Valsa Mali Miyabe et Yamada, Phytophthora capsici and phytophthora infestans. According to the invention, the bacillusamyloliquefaciens subsp Lh-1 and pesticide and chitosan oligosaccharide with dual effects of fertilizer and pesticide are combined into an Lh-1 microecological agent, the viable count is small, the dosage is small, the production process is simple, the cost is low, and the Lh-1 microecological agent has the dual functions of disease prevention and growth promotion. Nursery test proves that reduce the disease incidence of crop is reduced, especially for control of Verticillium dahliae, the disease incidence drops to 6%, and the control effect is significant. In addition, the Lh-1 microecological agent is a biological strain preparation, is completely free of a series of problems caused by use of chemical pesticides, thus being conducive to pollution-free production of crops, and farmers can have no need for pesticide or reduce the dosage of chemical pesticide, thus greatly saving the expenditure.

The invention relates to a planting method for dendrobium officinale. According to the planting method for the dendrobium officinale, raw materials such as peat soil, peanut shells, pine brans, pine bark, a small amount of bat excrement are manufactured into powder and are used as a substrate, high-temperature processing is conducted on the substrate at the temperature from 100 DEG C to 110 DEG C, superior varieties of dendrobium on a mountain are collected, sterilization processing is conducted on the varieties of dendrobium, then the varieties of dendrobium are sowed and transferred to a greenhouse after one year, the sunshine-shielding processing, and proper temperature and moisture control are adopted, a pollution-free bactericide is used for preventing the dendrobium officinale from getting the soft rot disease and the black spot disease, and prepared pure natural plant liquid is used as the insecticide, so that the planted dendrobium officinale which is plump, free of pesticides, hormones and chemical medicine pollution and excellent in quality is obtained, and the market requirements are met.

The invention discloses a novel bacillus methylotrophicus, which has the preservation register number of CGMCC No.6793, wherein the strain is separated from tobacco rhizosphere soil and is obtained by separating and purifying and screening strains. The invention further provides a fermentation method of the bacillus methylotrophicus, a fermentation product prepared by the method, as well as a biological agent based on the fermentation product and a preparation method thereof; the invention further provides a use of the biological agent in controlling a variety of plant diseases. According to the invention, the biological agent is good in environmental compatibility so that the number of effective living spores is more than 5,000,000,000/g, has high biological activity, and in particular has good control effect on tobacco bacterial wilt, wherein the control effect on tobacco bacterial wilt, soft rot of cabbage, rice bacterial leaf blight, rice bacterial stripe and cotton angular leaf spot is up to 80% or more.

The invention relates to bacillus subtilis M3 and an application thereof, and belongs to the technical field of biological pesticides. The produced strain is the bacillus subtilis M3; the preservation unit is the China General Microbiological Culture Collection Center; the address is Zhongguancun, Beijing, China; the preservation date is July 11, 2013; the preservation number is CGMCC NO.7911. The application of the bacillus subtilis M3 as a preparation for preventing and controlling Chinese cabbage clubroot, tomato scab, tomato root knot nematode diseases and konjak bacterial soft rot is also disclosed. The bacillus subtilis M3 disclosed by the invention achieves an obvious and stable prevention and control effect on the diseases, can be used for preparing the preparation for preventing and controlling the Chinese cabbage clubroot, the tomato scab, the tomato root knot nematode diseases and the konjak bacterial soft rot and has the advantages of high efficiency, nontoxicity, safety, no residue, bacteria activation, disease control, better growth promotion effect, better comprehensive character and easiness for industrialized production.

The invention discloses a bacillus methylothophicus strain for preventing and controlling pear ring rot and soft rot and application of the bacillus methylothophicus strain. The endophytic bacillus methylothophicus strain is separated from dendrobium huoshanense roots which are subjected to surface sterilization, and the strain has the broad-spectrum resistance on multiple plant pathogens and has the good prevention and control effect on pear fruit rot caused by the ring rot and the soft rot. The endophytic bacterium serving as a biocontrol bacterium has the advantages of the broad-spectrum bacterial resistance, the efficient safety and the like and is safe to pear fruits, low in large-scale production raw material, convenient to operate, stable in prevention and control effect and capable of effectively solving the problems of residual pesticides and environmental pollution which are brought by pesticide prevention and control over pear fruit diseases. Accordingly, the huge application potential on biological disease prevention and control after pear harvesting is achieved, and a good basic bacterial strain is provided for researching and developing microbial pesticides.

The invention discloses a microbicidal composition containing chloroisobromine cyanuric acid and antibiotics. The microbicidal compositio comprises an active component A and an active component B, wherein the active component A is chloroisobromine cyanuric acid, the active component B is any one of the following bactericides: validamycin, polyoxin and kasugamycin, and the weight percentage ratio of the active component A to the active component B is (1-60%):(1-40%). The microbicidal composition disclosed by the invention can be used for preventing and controlling various crop diseases, has distinct synergistic effect, broadens microbicidal spectrum, and has higher activity to sheath blight, rice blast, bacterial angular leaf spot, rice bacterial blight, virus diseases, soft rot, leaf mold, blight, downy mildew, Bakanae disease, banana leaf spot and apple rot. Furthermore, the dosage of the microbicidal composition is reduced, pesticide residues on crops is reduced, and environmental pollution is relieved.

The invention discloses a special polyelement microorganism fertilizer for preventing celery soft rot and a preparation method of the special polyelement microorganism fertilizer. The special polyelement microorganism fertilizer for preventing the celery soft rot comprises components of raw materials in parts by weight as follows: 0.8-1 part of bacillus amyloliquefaciens, 0.6-0.8 part of bacillus thuringiensis, 0.5-0.7 part of bacillus subtilis, 0.4-0.8 part of lactic acid bacteria, 1-3 parts of a matrix leavening agent, 30-35 parts of maize straw, 20-30 parts of turf, Chinese herbal medicinal ingredients and the like. The prepared special polyelement microorganism fertilizer for preventing the celery soft rot can reduce the morbidity of the celery soft rot greatly, a plant can grow more vigorously, the yield of celery is increased greatly, the quality is improved, probiotics in the soil are also increased, the fertilizer is pollution-free and low in residue, and the soil condition is improved greatly.

The invention discloses a cultivation method for intercropping green manure and konjak. The green manure and konjak are intercropped, wherein volatile substances secreted by the green manure can further kill germs, the substance secreted by root systems and microorganisms of the green manure plays an inhibition effect on soft-rot pathogenic bacteria, and plays an important role in lowering soft-rot diseases of the konjak; biological competition edges of the green manure are utilized to inhibit the growth of weeds, the land space is sufficiently utilized, the soil fertility is improved, the yield of the konjak is increased, the quality of the konkak is improved, and economic benefit of a farmer is increased; meanwhile, through a special selenium transfer mode, the selenium content of the konjak is increased, the content of selenium in per kilogram of the fresh konjak can reach 1.6mg/kg or above, the effect is good, and the cost is low at the same time.

The invention provides a special insecticide fertilizer for radish and a preparation method thereof. The special insecticide fertilizer for radish comprises the following raw materials by weight percent: 2-4% of lorsban, 6-10% of carbosulfan, 4-8% of phoxim, 25-35% of urea, 20-30% of calcium superphosphate, 15-25% of potassium sulfate and 5-15% of peat soil. The fertilizer and insecticide are organically combined through blending, agglomeration and granulation. The special insecticide fertilizer for radish and the preparation method thereof, which are provided by the invention, are novel and reasonable in design. The granular insecticide fertilizer is formed by granulating through a disk. The insecticide fertilizer can be administrated conveniently, the phyllotreta striolata and soft rot of radish can be obviously prevented and treated, the radish commercial property is increased, the economic benefit, social benefit and ecological benefit are all obvious, and the application value of the special insecticide fertilizer is higher.

The invention provides a mixture for controlling konjak diseases and insects, which relates to a mixture for controlling diseases and insects, in particular to a mixture for controlling diseases and insects in the growing process of konjak. The mixture consists of the following components mixed together by weight: 65 to 90 percent of high calcium powder, 7 to 25 percent of China tree powder and 3to 10 percent of potassium permanganate. By adopting the mixture for controlling the konjak diseases and insects, three years of fixed point tests show that the mixture can effectively control anomala corpulenta pests of the konjak and can also effectively control soft rot disease, southern blight and the like of the konjak, and the mixture does not influence the nature of soil and the planting of the konjak after being used for three years continuously. Besides, the mixture has low bio-residue and does not do harm to the health of people.

The invention relates to a technology for preventing and curing soft rot of konjak through self-made extraction of plant bactericide, and belongs to the field of plant disease prevention through the local pesticide extracting method. The extracting method is simple, feasible, convenient to use, and is suitable for being popularized and applied by the mass konjak growers as site preparation can be performed at any time. The environment-friendly plant bactericide has better soft rot prevention effect than chemical agents, and has the disease prevention effect reaching 86.3%, and during the growth of konjak, farmers only need to mix allicin extracted through the local method, 97% berberine extracted through the industrial method, honeysuckle leach liquor and isatis root leach liquor as per a certain proportion and according to own needs, so that continuous spreading of soft rot of field konjak can be effectively controlled, and the production can be remarkably guaranteed.

This invention pertains to a composition, which is a natural and organic pesticide, specifically for mitigating, controlling and treating fungicidal, virucidal and bactericidal pathogenic microorganisms in agricultural products such as root crops, fruits and vegetables. Examples of such disease-causing microorganisms are Mycosphaerella fijensis causing black Sigatoka disease in Cavendish (banana), Ralstonia solanacearum causing Moko disease in Cavendish (banana), Lasiodiplodia theobromae causing soft rot or fruit rot in crops and fruits, Fusarium oxysporum causing Panama wilt in fruits and crops, and many others. The composition is a fermented product of tropical plants, carbon source, protein (nitrogen) source, and a carrier agent. The fermented product may help strengthen the plant's immune system to fight pathogenic diseases. Since all the major constituents of the composition are generally regarded as safe, this natural pesticide is found to be non-toxic and safe to humans and animals, and environmentally benign.

The invention relates to the technical field of pesticides, and particularly relates to a bactericide composition which takes zhongshengrnycin and copper oxychloride as effective ingredients, has an obvious and enlarged bacteriocidal spectrum and no phytotoxicity, and is used for treating plant bacterial diseases as well as fungal diseases. The weight proportion of the zhongshengrnycin to the copper oxychloride is 1:5-60. The bactericide composition has obvious synergistic effect, high field prevention effect and a wide bacteriocidal spectrum, is used for preventing and treating cucumber bacterial angular leaf spot, cucumber downy mildew, tomato bacterial macula disease, tomato leaf mold, pumpkin soft rot, watermelon bacterial angular leaf spot, watermelon sponge, apple alternaria leaf spot, apple brown blotch, pear scabs, grape downy mildew, grape powdery mildew, anthracnose of grape, jujube rust diseases, jujube anthracnose, citrus canker, citrus scab, citrus anthracnose, peanut leaf spot diseases and peronophythora litchii, and has the advantages of good effect and reduction of prevention and treatment cost.

The invention discloses a method for preventing and controlling plant diseases and insect pests by intercropping of vegetables, and belongs to the field of modern pollution-free agricultural prevention and control of plant diseases and insect pests. Green onion and leaf vegetable are intercropped, a planting mode of 20 centimeters with a field is adopted, the green onion is planted in 5 rows and the row distance is 3 centimeters, the leaf vegetable is plant in 1 row and the row distance is 5 centimeters, 5 rows of green onion is planted with the seedling density per mu of 4,800 plants, then 1 row of leaf vegetable is planted, and the green onion and the leaf vegetable grow at intervals; the plant diseases and the insect pests can be controlled by using the growth habit difference of the green onion and the leaf vegetable and the strong pungent smell of the green onion; and the plant diseases and the insect pests of the green onion are original little, and the eggs of the plant diseases and the insect pests of the green onion planted field are relatively little, so the prodenia litura fabricius of the leaf vegetable is reduced by over 30 percent, the cabbage worm is reduced by 20 percent, the soft rot disease of the leaf vegetable is reduced by 18 percent, the downy mildew is reduced by 12 percent, and the generation of other plant diseases and insect pests of various leaf vegetables is also lightened obviously; therefore, the prevention and control effect which is better than that of pesticide application is achieved without applying pesticide. The pesticide pollution can be reduced, the agricultural insecticidal cost is lowered, the yield is improved, and the effects of greenness, safety and health are completely achieved.

The invention discloses an Erwinia carotovora pv. Carotovora strain Ecc 36 with the preservation number of CGMCC No. 11751; plant immune activator protein HrpNEccPR secreted by the Erwinia carotovora pv. Carotovora strain Ecc 36 has an effect of preventing and controlling plant soft rot. The invention also discloses a protein sequence of the plant immune activator protein HrpNEccPR and a gene sequence that expresses the protein, and further provides a method for obtaining the plant immune activating protein. According to the invention, an activator protein crude product generated by the selected strain with the preservation number of CGMCC No. 11751 has a good antagonistic effect for plants, especially for Chinese cabbage soft rot, and the activator protein crude product can effectively prevent and control the occurrence of the Chinese cabbage soft rot.

The invention relates to a cherry preservative and fresh-keeping microbial preparation and a preparation method and application thereof. The cherry preservative and fresh-keeping microbial preparationis prepared from bacillus velezensis TA-3-BV which is preserved in China General Microbiological Culture Collection Center on July 17, 2020, and the preservation number is CGMCC NO. 20398. The microbial preparation disclosed by the invention can effectively inhibit mycelial growth and spore germination of pathogenic bacteria rhizopus stolonifer and effectively inhibit morbidity of soft rot of sweet cherries at the same time, after the picked cherries are soaked in bacillus velezensis TA-3-BV bacterial suspension, the fruit spoilage process can be delayed, and fruit spoilage is delayed by 2-3days. Meanwhile, through treatment of the bacillus velezensis TA-3-BV, the disease resistance and oxidation resistance of plants can be induced, the basic immunity of cherry fruits is triggered, and anew method is provided for preservation and fresh keeping of the harvested sweet cherries.

The invention particularly relates to Lysobacter enzymogenes 1-T-1-4 and application thereof. The Lysobacter enzymogenes 1-T-1-4 can be prepared into aquae or wettable powder and is used for preventing and treating diseases such as panax notoginseng root rot and amorphophallus konjac soft rot through acting on panax notoginseng or amorphophallus konjac.

The present invention relates to a compound for preventing and treating kiwi fruit soft rot and derivatives thereof. The compound for preventing and treating the kiwi fruit soft rot and the derivatives thereof can be prepared into various agricultural conventional dosage forms. The compound and the derivatives thereof can effectively prevent and treat the kiwi fruit soft rot.

The invention provides a fast propagation method for seed konjak. According to the invention, corm and rhizome of konjak are distributed with a great number of latent buds; when the corm of the konjak is used as a seed, only terminal buds are germinated into seedlings, while one part of the latent buds are differentiated into a root system, and the other part of the latent buds are latent and do not germinate. The method provided by the invention stimulates the latent buds to germinate into seedlings mainly through stripping and slicing of the corm, matches with seedbed management measures like use of a cut healing agent, fine adjustment of physicochemical environment of soil, improvement of microbial communities and control of the occurrence of soft rot disease, improves an original propagation coefficient of 3 to 5 times to 15 to 20 times, realizes fast propagation of the seed konjak, and is applicable to industrial production.

The invention provides Bacillus subtilis ML1 which is preserved in China Center for Type Culture Collection on May 5, 2014, with an accession number of CCTCC No: M2014181. A breeding method for the Bacillus subtilis ML1 comprises the following steps: adding sterile water into the tissue fluid of leaves of Myricaria laxiflora and carrying out dilution to obtain 7 concentration gradients; coating medium plates with the diluted tissue fluid, invertedly placing the medium plates in a thermotank and carrying out culture at 30 DEG C; after bacterium colonies have grown on the plates, carrying out shake cultivation at a constant temperature of 30 DEG C so as to obtain bacterium culture solutions, sterilizing the bacterium culture solutions, mixing the bacterium culture solutions with glycerin and preserving the obtained mixture in a refrigerator with a temperature of -86 DEG C; subjecting the preserved Bacillus subtilis strain to streak inoculation in a slant medium and carrying out culture at 30 DEG C for 12 h; and subjecting the strain to streak inoculation in a medium and carrying out culture at 30 DEG C for 18 h so as to obtain the Bacillus subtilis ML1. The Bacillus subtilis ML1 is applied to prevention and treatment of soft rot disease of konjak and provides a novel option for development of a novel biological pesticide variety and novel biological control inocula.

The invention discloses a rapid separation method of antagonistic bacteria against pathogens of konjac soft rot disease, which comprises the following steps: A) taking konjac tissues infected by the soft rot disease; B) inoculating the tissues at the interface of diseased and healthy tissues onto a nutrient agar culture medium after carrying out surface disinfection; C) streaking the bacteria grown out on the sides of the tissues, and selecting a single colony for purification and preservation; D) adopting a distinctive Erwinia crystal violet polypectin selective culture medium (CVP) to screen the pathogens of the konjac soft rot disease; E) adopting the point connection method to carry out primary screening of antagonistic bacteria; and F) adopting the oxford cup method to carry out secondary screening of the antagonistic bacteria. The method has simple operation and easy operation, and can rapidly and effectively screen out the antagonistic bacteria against the pathogens of the konjac soft rot disease and simultaneously provide a concept for rapid screening of the antagonistic bacteria against other crop diseases.