61 results about "Soft rot disease" patented technology

The invention discloses application of Indel marker dosage typing in detection of sweet potato soft rot resistance, and belongs to the technical field of plant genetic engineering. The Indel molecular marker related to the sweet potato soft rot resistance is obtained through screening, the nucleotide sequence of the Indel molecular marker is shown as SEQ ID NO.2, and an 8bp sequence is inserted into or deleted from the 161st site of the sequence shown as SEQ ID NO.2. Experiments find that target bands of amplification products are obtained through amplification of the Indel molecular marker and electrophoresis, dosage values of two genotypes are obtained according to gray values of the dyed electrophoretic bands, and results show that the insertion type Indel molecular marker is positively correlated with the sweet potato soft rot resistance. The method is of great significance to the dose effect research and molecular assisted breeding of sweet potato gene functions.

The invention relates to the technical field of biology, in particular to pure bacillus simplex ZF535 capable of producing exopolysaccharides and application of the pure bacillus simplex ZF535. The preservation registration number of the simple pure bacillus ZF535 disclosed by the invention in the China General Microbiological Culture Collection Center (CGMCC) is CGMCC No: 34733. Pot experiment results show that leaf spraying or root irrigation treatment of exopolysaccharides of the strain ZF535 has a remarkable induced disease-resistant effect on cucumber angular spots, and the prevention effects are 56.59% and 60.43% respectively; the strain ZF535 exopolysaccharide has a remarkable induced disease-resistant effect on the cucumber soft rot disease through leaf spraying or root irrigation treatment, and the prevention effects are 54.35% and 65.11% respectively. In conclusion, the strain ZF535 is a simple pure bacillus with potential biocontrol application potential for inducing plant disease resistance, and has a better biocontrol effect.

The invention relates to a special microbial agent fertilizer for konjak, and aims to break through the industrial bottlenecks of high incidence of soft rot, damage caused by waterlogging stress and glucomannan synthesis obstacles in konjak cultivation. According to the core technical scheme, the activity of soft rot fungus pectinase is inhibited in a targeted mode through clostridium butyricum, and efficient polymerization of gulcomannan molecular chains is driven in cooperation with a hydroxyproline precursor provided by fish meal; a sulfonic acid group of biochemical potassium fulvate is used for blocking waterlogging free radical poison, and a brown sugar-trehalose carbon source ratio is optimized to stabilize cell osmotic pressure in an expansion period; and a supplementary application mechanism based on a precipitation threshold (greater than or equal to 10mm) and a copper preparation application isolation regulation are established in a matched manner. According to the technology, the three-effect synergy that the soft rot prevention and control rate is larger than 90%, the glucomannan viscosity improvement rate is larger than 70% and the waterlogging survival rate is larger than 85% is achieved, and the core restriction of high-yield and high-quality production of the konjak is systematically solved.

The invention discloses a high-performance antagonistic bacterium RQH1 and application of the high-performance antagonistic bacterium RQH1 in prevention and control of amorphophallus konjac soft rot. The high-performance antagonistic bacterium RQH1 is preserved in China Center for Type Culture Collection, the preservation number is CCTCC NO. M 2023719, the preservation address is Wuhan University, Wuhan, China, and the preservation time is May 10, 2023. According to the invention, a high-performance antagonistic bacterium RQH1 is obtained by directional screening in a healthy konjak plant body, and the strain not only shows broad-spectrum and high-efficiency bacteriostatic activity on various konjak soft rot pathogenic bacteria, but also obviously inhibits the pathogenic bacteria from growing and infecting; meanwhile, various growth promoting functions such as phosphorus dissolving, potassium dissolving and nitrogen fixing can be achieved, the soil nutrient condition can be improved, and konjak plants can be promoted to grow robustly. In addition, the composition and antibacterial mechanism of volatile metabolites of the RQH1 strain are systematically analyzed. Therefore, a high-value strain resource which is multifunctional, easy to culture and high in adaptability is provided for green prevention and control of the soft rot of the konjak, and various defects in the prior art are effectively overcome.

This invention relates to the field of agricultural pest control, and particularly to a compound nanogel sustained-release agent, its preparation method, and its application. This invention provides a compound nanogel sustained-release agent comprising bromonitol, a synergist, a carrier material, and a cross-linking agent, wherein the synergist includes one or more of kasugamycin, amino oligosaccharides, thiazolium zinc, and copper hydroxide. This invention prepares a nanogel sustained-release agent by compounding bromonitol and the synergist, which has significant preventive effects against plant bacterial diseases such as bacterial wilt, bacterial angular leaf spot, canker, and soft rot. Furthermore, the compound nanogel sustained-release agent of this invention has small particle size, stable physicochemical properties under cold and heat, good dispersibility in water, no particle agglomeration, no particle size increase, a decomposition rate of less than 5%, a long-lasting effect, good safety, is not prone to inducing drug resistance, requires a small dosage, and simultaneously increases yield and efficiency.

The invention belongs to the technical field of molecular biology, and particularly relates to a molecular marker related to soft rot resistance of zantedeschia aethiopica and application of the molecular marker. The molecular marker is located at the 68676823rd site of a zantedeschia No.4 chromosome and can be used for identifying whether a zantedeschia variety has resistance to soft rot or not. The identification of the common callalily soft rot resistance is enriched and simplified, and the breeding period of a soft rot resistance material is shortened. And a technical support is provided for germplasm resource utilization and molecular breeding of the calla lily.

The invention belongs to the technical field of gene engineering, and particularly relates to application of a PtZFP20 gene in regulating soft rot resistance of pinellia ternate. The invention discovers that PtZFP20 is closely related to the soft rot resistance of pinellia ternate for the first time, and the soft rot resistance of pinellia ternate can be improved by increasing the expression quantity of PtZFP20. The PtZFP20 gene provides a powerful technical support for cultivating a new disease-resistant variety of pinellia ternate, and can be widely applied to breeding and utilization of the disease-resistant variety of pinellia ternate.

The mass ratio of quinolinic acid to clindamycin is (1-3): (7-9), after the clindamycin and the quinolinic acid are used in a combined mode, a synergistic effect is achieved in the aspect of prevention and treatment of the Chinese cabbage soft rot, and compared with the concentration required for bacteriostasis of single clindamycin or single quinolinic acid, the concentration required for bacteriostasis of the clindamycin and the quinolinic acid is reduced, so that the effect of preventing and treating the Chinese cabbage soft rot is improved. After compounding, the use amount of the two is greatly reduced, and the composition has a remarkable control effect on the soft rot of the Chinese cabbage.

The present application provides a kind of diandong yam root endophytic fungus fragile fruit pringlea DHJ068 and its application, it is related to crop disease prevention and treatment technical field.The diandong yam root endophytic fungus fragile fruit pringlea DHJ068 is preserved in China typical culture collection, and the preservation number is CCTCC NO:M 2025748, and the fermentation broth extract of the bacteria can be applied to crop disease prevention and treatment.The present application overcomes the shortcomings of the prior art, the fermentation broth extract of diandong yam root endophytic fungus fragile fruit pringlea DHJ068 can effectively inhibit the growth of Chinese cabbage soft rot bacteria and cucumber bacterial angular spot bacteria, and achieve certain biological control purposes.

The invention relates to the technical field of medicinal chemistry, and discloses a thymol derivative as well as a preparation method and application thereof. The thymol ethyl, propyl, butyl azole and piperazine derivative provided by the invention is used as a bacteriostatic agent for resisting phytopathogen and is used for preparing pesticides; the prepared pesticide can be used for preventing and treating plant diseases such as cucumber damping-off, rice damping-off, cotton damping-off, watermelon damping-off, sclerotinia rot of colza, botrytis cinerea, cucumber gray mold, grape gray mold, wheat scab, rice blast, wheat sharp eyespot, wheat stem rot, sweet potato soft rot and lily wilt.

This application discloses an indoor artificial rearing method for the rice stem borer, belonging to the field of insect rearing technology. This method addresses the problems of drowning in young larvae and frequent disease outbreaks in older larvae caused by homogeneous rearing across all generations. It constructs a constant-temperature environment and prepares granular dry feed for young larvae and slightly dehydrated flake feed for older larvae based on differences in larval mouthparts. A heterogeneous three-dimensional rearing device is used. In the young larvae stage, a slow-release substrate at the bottom maintains a vertical humidity gradient, and layered feeding prevents mechanical damage to the larvae. In the older larvae stage, substrate-free bare-bottom rearing and full-volume replacement feeding are employed. Timely removal of filamentous netting blocks the breeding environment for soft rot. Furthermore, the method combines pupation induction with corrugated paper induction and non-contact moisturizing, humidification during the adult dark period to delay gelatinous hardening, and overall substrate replacement during the light period to achieve damage-free egg mass harvesting. This application, through precise age-based management, significantly improves the survival rate and population propagation efficiency of the rice stem borer across all generations.

This invention discloses a method for preparing and applying nanoliposomes, relating to the fields of kiwifruit soft rot control and kiwifruit preservation. The preparation method includes the following steps: mixing thymol, cholesterol, and oleic acid to obtain an oil phase; mixing polyethylene glycol, lauryl ether phosphate, an emulsifying dispersant, and water to obtain an aqueous phase; mixing the oil phase and the aqueous phase, performing high-speed emulsification and shearing followed by low-speed stirring to obtain nanoliposomes. This invention uses cholesterol and oleic acid as wall materials and polyethylene glycol as a modifying material to successfully load thymol into nanoliposomes via microemulsification, achieving highly efficient encapsulation of thymol. The obtained thymol-containing nanoliposomes exhibit excellent performance and can be used to prepare formulations for controlling kiwifruit soft rot, demonstrating excellent control effects against the disease.

The application belongs to the technical field of molecular biology, and particularly relates to a molecular marker related to soft rot resistance of Zantedeschia and application thereof. The molecular marker is located at the 68676823th position of the fourth chromosome of Zantedeschia, and can be used for identifying whether a Zantedeschia variety has soft rot resistance. The application enriches and simplifies the identification of the soft rot resistance of Zantedeschia, shortens the breeding cycle of the soft rot resistance material, and provides technical support for Zantedeschia germplasm resource utilization and molecular breeding.

This invention belongs to the field of fermentation engineering and plant disease control technology, specifically relating to the application of a Bacillus endophyticus compound inoculant fermentation broth in the control of plant diseases. The Bacillus endophyticus compound inoculant disclosed in this invention exhibits antibacterial effects against anthracnose in peppers, blight in peppers, gray mold in tomatoes, wilt in watermelons, wilt in cucumbers, verticillium wilt in cotton, black spot in peppers, root rot in carrots, soft rot in taro, and bacterial wilt in peppers, with inhibition rates of (75.37±1.44), (71.00±1.41)%, (83.14±0.96)%, (74.70±1.09)%, (69.21±1.04)%, (78.31±1.39)%, (73.06±0.73)%, (75.49±0.89)%, (46.79±1.57)%, and (73.82±0.51)%, respectively.

The application discloses a plant Lactiplantibacillus plantarum Yi-BC5 with a preservation number of GDMCC No: 67799, which shows strong inhibition on Pectobacterium carotovorum and Penicillium digitatum in vitro and in vivo, can significantly reduce the incidence and lesion diameter of pepper bacterial soft rot and citrus green mold; the Lactiplantibacillus plantarum Yi-BC5 can produce extracellular polysaccharide and other antibacterial metabolites, and the bacterial body and / or bacterial metabolite can be used for preparing products for inhibiting Pectobacterium carotovorum and / or Penicillium digitatum, and can be used for preparing products for preventing and treating bacterial diseases (including but not limited to pepper bacterial soft rot) and / or fungal diseases (including but not limited to citrus green mold) of fruits and vegetables. The Lactiplantibacillus plantarum Yi-BC5 can simultaneously control bacterial diseases and fungal diseases of fruits and vegetables after harvesting, has excellent disease control performance, provides an excellent antagonistic bacterial resource for postharvest preservation of fruits and vegetables, and lays a foundation for subsequent development of a new type of microbial bactericide.

The invention discloses application of cinnamyl alcohol in prevention and treatment of sweet potato soft rot, and belongs to the technical field of biology. Experiments find that low-concentration cinnamyl alcohol (200-400 [mu] g.ml <-1 >) can significantly inhibit soft rot pathogenic bacteria, can help eliminate or relieve sweet potato soft rot, provides a new way for safe and efficient prevention and treatment of sweet potato fungal diseases, provides a theoretical basis for cinnamyl alcohol development of a green biological fresh-keeping agent for sweet potato postharvest storage, and has broad application prospects. The method has important application value for reducing dependence on chemical pesticides and guaranteeing food safety in sweet potato production.

The application discloses application of florfenicol in prevention and treatment of plant bacterial diseases and an inhibitor. The application provides a new application of florfenicol, which can be used for preventing and treating Xanthomonas oryzae pv. oryzae, Xanthomonas campestris pv. oryzae, Xanthomonas axonopodis pv. citri, Pseudomonas syringae pv. tabaci, Pseudomonas syringae pv. lachrymans, Pseudomonas syringae pv. tomato, Pseudomonas syringae pv. lactucae, Pseudomonas syringae pv. actinidiae, Pseudomonas syringae pv. angulata and the like, and provides a new idea for preventing and treating the above-mentioned plant bacterial diseases. Experiments prove that florfenicol has high bactericidal activity on the above-mentioned plant bacterial diseases.

This invention relates to the field of agricultural product storage, and more particularly to a fumigation method for preventing soft rot in agricultural products. The method involves fumigation using a cellulose-based material as a carrier, perillaldehyde and / or salicylaldehyde as a base, and the addition of isobutyric acid and / or 2-methylbutyric acid. This invention effectively inhibits and kills *Rhizopus spp.*, the pathogen causing soft rot in agricultural products, maintaining the physiological quality and nutritional value of agricultural products during storage, and significantly extending the storage period. This invention is highly effective, simple to operate, environmentally friendly and safe, and easily scaled up and industrialized.

This application discloses an indoor artificial rearing method for the rice stem borer, belonging to the field of insect rearing technology. This method addresses the problems of drowning in young larvae and frequent disease outbreaks in older larvae caused by homogeneous rearing across all generations. It constructs a constant-temperature environment and prepares granular dry feed for young larvae and slightly dehydrated flake feed for older larvae based on differences in larval mouthparts. A heterogeneous three-dimensional rearing device is used. In the young larvae stage, a slow-release substrate at the bottom maintains a vertical humidity gradient, and layered feeding prevents mechanical damage to the larvae. In the older larvae stage, substrate-free bare-bottom rearing and full-volume replacement feeding are employed. Timely removal of filamentous netting blocks the breeding environment for soft rot. Furthermore, the method combines pupation induction with corrugated paper induction and non-contact moisturizing, humidification during the adult dark period to delay gelatinous hardening, and overall substrate replacement during the light period to achieve damage-free egg mass harvesting. This application, through precise age-based management, significantly improves the survival rate and population propagation efficiency of the rice stem borer across all generations.

This invention discloses a "vaccine-type" bacteriocin, Yi-VD14, with the amino acid sequence VDFGPHGLLPIRPI, which can induce plant immune responses and enhance plant disease resistance. Taking chili peppers as an example, this bacteriocin significantly improves the storage quality of chili peppers by delaying vitamin C decomposition and chlorophyll degradation and reducing malondialdehyde accumulation. Furthermore, it induces plant immune responses and enhances plant disease resistance by regulating fruit fat metabolism, inhibiting cell membrane degradation, and improving the biosynthesis of plant disease-resistant metabolites. It also increases the activity of disease-resistant enzymes in chili pepper fruits and reshapes the microbial community structure on the surface of the fruits. Ultimately, it enhances the plant's resistance to pathogenic microorganisms from multiple dimensions, reducing the incidence of chili pepper soft rot and minimizing post-harvest losses. This bacteriocin can be used not only to prepare products for controlling chili pepper soft rot but also for controlling other fruit and vegetable diseases, showing promising application prospects in the fruit and vegetable planting and post-harvest distribution industry chain.

The present application belongs to the field of microbial technology, and discloses a compost bacillus DX-10 and application thereof. The present application provides a compost bacillus, which is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20251073. The strain has a good prevention and treatment effect on kiwi soft rot, and shows excellent inhibition on the pathogenic bacteria of kiwi soft rot, such as grape Coniothyrium and Diaporthe phaseolorum. The strain provides a strain resource for preparing a biocontrol agent, can alleviate the environmental hazards caused by excessive use of chemical agents, and is expected to become an important tool for developing green control drugs for kiwi soft rot.

The invention discloses a preparation method of a novel coumarin derivative containing a benzylamine structure and application of the novel coumarin derivative in the aspects of virus resistance, bacteriostasis and plant growth promotion. The novel coumarin derivative containing the benzylamine structure is characterized in that the general formula of the derivative is shown in the specification, wherein R1 is halogen, methoxyl, nitryl, methyl, cyano, tert-butyl or disubstitution of any combination of the above substituent groups. The derivative disclosed by the invention has a relatively good inhibition effect on rice bacterial leaf blight, tobacco bacterial wilt, potato soft rot, tobacco mosaic virus disease and cucumber mosaic virus disease. The compound II-8 has the activity of promoting plant growth.

This invention belongs to the field of gene technology and discloses a bacterial strain B11 antagonistic to soft rot disease of Amorphophallus muelleri. Isolated from the rhizosphere soil of Amorphophallus muelleri, it was identified as the golden subspecies of *Pseudomonas aeruginosa*. This strain effectively inhibits the growth of the soft rot pathogen Pcc, reducing the incidence and disease index of soft rot in Amorphophallus muelleri. It also possesses the ability to dissolve organophosphates and produce siderophores, and exhibits broad-spectrum antagonistic activity against various fungal diseases of Amorphophallus muelleri, including white mold, leaf spot, and dry rot. Whole-genome analysis shows that the B11 genome is 6.77 Mb in size, with a GC content of 62.81%, encoding 5,964 genes, including gene clusters related to antibacterial activity such as phenazine, nitropyrrolizin, and siderophores. This strain has good development and application potential in the control of soft rot in Amorphophallus muelleri.

Abstract:　Problem: To provide a soil-transmissible plant disease control agent or similar that can safely be used as a biological control agent.　Solution: A soil-transmissible plant disease control agent or similar, such as a vegetable bacterial wilt control agent, can be provided by using, as an active ingredient, live bacterial cells of a novel strain of a bacterium of the Bacillus or Fictibacillus genus with a heretofore unknown soil-transmissible plant disease controlling action, or a cultured product including said live bacterial cells. This control agent is characterized by being able, through a foliage application treatment, to control bacterial wilt and / or bacterial soft rot caused by the Ralstonia solanacearum species complex, which are soil-borne bacteria.　Selected drawing: none

The invention provides an ethyl ferulate liposome as well as a preparation method and application thereof in resisting soft rot of kiwi fruits. According to the application disclosed by the invention, the differences of three compounds of ferulic acid, methyl ferulate and ethyl ferulate on in-vitro growth inhibition of kiwi fruit soft rot pathogenic bacteria-botryosphaeria dothidea mycelia are screened and compared, and the condition that the ethyl ferulate has a better inhibition effect on the botryosphaeria dothidea is determined; liquid grease and solid grease are used as wall materials, ethyl ferulate is used as a core material, and the nano ethyl ferulate liposome is prepared by adopting a melt emulsification ultrasonic method. The prepared nano ethyl ferulate liposome is uniform in particle size, and compared with non-embedded ethyl ferulate, the bacteriostatic activity and stability of the ethyl ferulate liposome are remarkably improved, and the nano ethyl ferulate liposome has a better application prospect in resistance to soft rot of kiwi fruits.

The application discloses a pathogenic protein of a soft rot pathogen of Pleurotus eryngii and an encoding gene thereof. The application provides a protein obtained from a Beijing E. coli, which is named as protein 1408 and is a protein shown in sequence 1 in a sequence list. A DNA molecule encoding the protein 1408 is also called as gene 1408. The application also protects the application of the protein 1408, which is as follows: regulating the pathogenicity of the Beijing E. coli; regulating the polysaccharide content of the Beijing E. coli. The inventors of the application first report the soft rot of the Pleurotus eryngii in Beijing and find the Beijing E. coli causing the pathogenicity. After the gene 1408 in the Beijing E. coli is knocked out, the content of the exocellular polysaccharide is reduced and the pathogenicity is reduced. The application has great application value for the pathogenic mechanism research of the Beijing E. coli and the prevention and control of the soft rot.

The invention discloses a pinellia tuber soft rot fungus infection dynamic detection method, which comprises: applying a pH indicator to a to-be-detected surface of a plant tissue sample, and carrying out timing observation after the application; the color change range of the pH indicator at least covers pH 5.0-pH 7.0. The invention provides a dynamic monitoring system combining chromogenic reaction and pH value change, the method can be used for dynamic detection of soft rot fungus infection of pinellia tuber tubers, the method is simple and convenient in detection process operation, does not need complex equipment, is suitable for laboratory environment and can also be used for rapid field detection, the detection efficiency is improved, and the detection scene is expanded.

The invention relates to the technical field of pathogenic bacteria culture and preservation. The invention provides a culture medium and a preservation solution for pathogenic bacteria of soft rot of konjak and a use method of the culture medium and the preservation solution. The culture medium is prepared from the following raw materials in parts by weight: 2.5-3.5 parts of beef extract, 9-11 parts of peptone, 4.5-5.5 parts of sodium chloride, 4-6 parts of agar, 4-6 parts of whole konjak powder and 950-1050 parts of water. According to the invention, a konjac soft rot pathogen culture medium formula and a preservation method are improved by utilizing an interaction relationship between konjac soft rot pathogens and konjac. The amorphophallus konjac whole powder is used for replacing part of agar powder and other nutrient substances, the soft rot pathogenic bacteria can be efficiently cultured, and meanwhile, the preservation time of the strain can be remarkably prolonged in cooperation with a specific preservation solution and a preservation method.

This invention discloses the application of Indel marker dose genotyping in detecting sweet potato soft rot resistance, belonging to the field of plant genetic engineering technology. This invention obtains an Indel molecular marker associated with sweet potato soft rot resistance through screening, with its nucleotide sequence shown in SEQ ID NO.2. The sequence shown in SEQ ID NO.2 has an 8bp insertion or deletion starting from position 161. Experiments revealed that by amplifying the Indel molecular marker and obtaining the target band of the amplification product by electrophoresis, the dose values ​​of the two genotypes were obtained based on the grayscale value of the stained electrophoretic band. The results showed a positive correlation between the inserted Indel molecular marker and sweet potato soft rot resistance, which is of great significance for dose-response studies of sweet potato gene function and molecular-assisted breeding.