19 results about "Soft rot disease" patented technology

This application discloses an indoor artificial rearing method for the rice stem borer, belonging to the field of insect rearing technology. This method addresses the problems of drowning in young larvae and frequent disease outbreaks in older larvae caused by homogeneous rearing across all generations. It constructs a constant-temperature environment and prepares granular dry feed for young larvae and slightly dehydrated flake feed for older larvae based on differences in larval mouthparts. A heterogeneous three-dimensional rearing device is used. In the young larvae stage, a slow-release substrate at the bottom maintains a vertical humidity gradient, and layered feeding prevents mechanical damage to the larvae. In the older larvae stage, substrate-free bare-bottom rearing and full-volume replacement feeding are employed. Timely removal of filamentous netting blocks the breeding environment for soft rot. Furthermore, the method combines pupation induction with corrugated paper induction and non-contact moisturizing, humidification during the adult dark period to delay gelatinous hardening, and overall substrate replacement during the light period to achieve damage-free egg mass harvesting. This application, through precise age-based management, significantly improves the survival rate and population propagation efficiency of the rice stem borer across all generations.

This invention discloses a method for preparing and applying nanoliposomes, relating to the fields of kiwifruit soft rot control and kiwifruit preservation. The preparation method includes the following steps: mixing thymol, cholesterol, and oleic acid to obtain an oil phase; mixing polyethylene glycol, lauryl ether phosphate, an emulsifying dispersant, and water to obtain an aqueous phase; mixing the oil phase and the aqueous phase, performing high-speed emulsification and shearing followed by low-speed stirring to obtain nanoliposomes. This invention uses cholesterol and oleic acid as wall materials and polyethylene glycol as a modifying material to successfully load thymol into nanoliposomes via microemulsification, achieving highly efficient encapsulation of thymol. The obtained thymol-containing nanoliposomes exhibit excellent performance and can be used to prepare formulations for controlling kiwifruit soft rot, demonstrating excellent control effects against the disease.

The application belongs to the technical field of molecular biology, and particularly relates to a molecular marker related to soft rot resistance of Zantedeschia and application thereof. The molecular marker is located at the 68676823th position of the fourth chromosome of Zantedeschia, and can be used for identifying whether a Zantedeschia variety has soft rot resistance. The application enriches and simplifies the identification of the soft rot resistance of Zantedeschia, shortens the breeding cycle of the soft rot resistance material, and provides technical support for Zantedeschia germplasm resource utilization and molecular breeding.

This invention belongs to the field of fermentation engineering and plant disease control technology, specifically relating to the application of a Bacillus endophyticus compound inoculant fermentation broth in the control of plant diseases. The Bacillus endophyticus compound inoculant disclosed in this invention exhibits antibacterial effects against anthracnose in peppers, blight in peppers, gray mold in tomatoes, wilt in watermelons, wilt in cucumbers, verticillium wilt in cotton, black spot in peppers, root rot in carrots, soft rot in taro, and bacterial wilt in peppers, with inhibition rates of (75.37±1.44), (71.00±1.41)%, (83.14±0.96)%, (74.70±1.09)%, (69.21±1.04)%, (78.31±1.39)%, (73.06±0.73)%, (75.49±0.89)%, (46.79±1.57)%, and (73.82±0.51)%, respectively.

The application discloses a plant Lactiplantibacillus plantarum Yi-BC5 with a preservation number of GDMCC No: 67799, which shows strong inhibition on Pectobacterium carotovorum and Penicillium digitatum in vitro and in vivo, can significantly reduce the incidence and lesion diameter of pepper bacterial soft rot and citrus green mold; the Lactiplantibacillus plantarum Yi-BC5 can produce extracellular polysaccharide and other antibacterial metabolites, and the bacterial body and / or bacterial metabolite can be used for preparing products for inhibiting Pectobacterium carotovorum and / or Penicillium digitatum, and can be used for preparing products for preventing and treating bacterial diseases (including but not limited to pepper bacterial soft rot) and / or fungal diseases (including but not limited to citrus green mold) of fruits and vegetables. The Lactiplantibacillus plantarum Yi-BC5 can simultaneously control bacterial diseases and fungal diseases of fruits and vegetables after harvesting, has excellent disease control performance, provides an excellent antagonistic bacterial resource for postharvest preservation of fruits and vegetables, and lays a foundation for subsequent development of a new type of microbial bactericide.

This application discloses an indoor artificial rearing method for the rice stem borer, belonging to the field of insect rearing technology. This method addresses the problems of drowning in young larvae and frequent disease outbreaks in older larvae caused by homogeneous rearing across all generations. It constructs a constant-temperature environment and prepares granular dry feed for young larvae and slightly dehydrated flake feed for older larvae based on differences in larval mouthparts. A heterogeneous three-dimensional rearing device is used. In the young larvae stage, a slow-release substrate at the bottom maintains a vertical humidity gradient, and layered feeding prevents mechanical damage to the larvae. In the older larvae stage, substrate-free bare-bottom rearing and full-volume replacement feeding are employed. Timely removal of filamentous netting blocks the breeding environment for soft rot. Furthermore, the method combines pupation induction with corrugated paper induction and non-contact moisturizing, humidification during the adult dark period to delay gelatinous hardening, and overall substrate replacement during the light period to achieve damage-free egg mass harvesting. This application, through precise age-based management, significantly improves the survival rate and population propagation efficiency of the rice stem borer across all generations.

This invention discloses a "vaccine-type" bacteriocin, Yi-VD14, with the amino acid sequence VDFGPHGLLPIRPI, which can induce plant immune responses and enhance plant disease resistance. Taking chili peppers as an example, this bacteriocin significantly improves the storage quality of chili peppers by delaying vitamin C decomposition and chlorophyll degradation and reducing malondialdehyde accumulation. Furthermore, it induces plant immune responses and enhances plant disease resistance by regulating fruit fat metabolism, inhibiting cell membrane degradation, and improving the biosynthesis of plant disease-resistant metabolites. It also increases the activity of disease-resistant enzymes in chili pepper fruits and reshapes the microbial community structure on the surface of the fruits. Ultimately, it enhances the plant's resistance to pathogenic microorganisms from multiple dimensions, reducing the incidence of chili pepper soft rot and minimizing post-harvest losses. This bacteriocin can be used not only to prepare products for controlling chili pepper soft rot but also for controlling other fruit and vegetable diseases, showing promising application prospects in the fruit and vegetable planting and post-harvest distribution industry chain.

The present application belongs to the field of microbial technology, and discloses a compost bacillus DX-10 and application thereof. The present application provides a compost bacillus, which is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20251073. The strain has a good prevention and treatment effect on kiwi soft rot, and shows excellent inhibition on the pathogenic bacteria of kiwi soft rot, such as grape Coniothyrium and Diaporthe phaseolorum. The strain provides a strain resource for preparing a biocontrol agent, can alleviate the environmental hazards caused by excessive use of chemical agents, and is expected to become an important tool for developing green control drugs for kiwi soft rot.

This invention belongs to the field of gene technology and discloses a bacterial strain B11 antagonistic to soft rot disease of Amorphophallus muelleri. Isolated from the rhizosphere soil of Amorphophallus muelleri, it was identified as the golden subspecies of *Pseudomonas aeruginosa*. This strain effectively inhibits the growth of the soft rot pathogen Pcc, reducing the incidence and disease index of soft rot in Amorphophallus muelleri. It also possesses the ability to dissolve organophosphates and produce siderophores, and exhibits broad-spectrum antagonistic activity against various fungal diseases of Amorphophallus muelleri, including white mold, leaf spot, and dry rot. Whole-genome analysis shows that the B11 genome is 6.77 Mb in size, with a GC content of 62.81%, encoding 5,964 genes, including gene clusters related to antibacterial activity such as phenazine, nitropyrrolizin, and siderophores. This strain has good development and application potential in the control of soft rot in Amorphophallus muelleri.

Abstract:　Problem: To provide a soil-transmissible plant disease control agent or similar that can safely be used as a biological control agent.　Solution: A soil-transmissible plant disease control agent or similar, such as a vegetable bacterial wilt control agent, can be provided by using, as an active ingredient, live bacterial cells of a novel strain of a bacterium of the Bacillus or Fictibacillus genus with a heretofore unknown soil-transmissible plant disease controlling action, or a cultured product including said live bacterial cells. This control agent is characterized by being able, through a foliage application treatment, to control bacterial wilt and / or bacterial soft rot caused by the Ralstonia solanacearum species complex, which are soil-borne bacteria.　Selected drawing: none

The application discloses a Trichoderma guepinii GUDJ16 and application thereof, and belongs to the technical field of microorganisms. The preservation number of the Trichoderma guepinii GUDJ16 screened by the application is CCTCC NO: M 20252004. The Trichoderma guepinii GUDJ16 has a prevention and treatment effect on kiwi soft rot, and the inhibition rate of the Trichoderma guepinii GUDJ16 on Botryosphaeria dothidea can reach 77.15%. The Trichoderma guepinii GUDJ16 also has an inhibition effect on Fusarium solani, Fusarium graminearum, Fusarium asiaticum, Rhizoctonia solani, Sclerotium rolfsii, Colletotrichum musae, Passalora fulva and Alternaria tenuissima. It is found through disease prevention and treatment effect verification on in-vitro fruits infected with kiwi soft rot that the prevention and treatment effect of the Trichoderma guepinii GUDJ16 on kiwi soft rot can reach 61.26% after treatment, and therefore the Trichoderma guepinii GUDJ16 has high application value in the field of kiwi soft rot prevention and treatment.

This invention discloses a *Rummeliibacillus* sp. and its application, relating to the field of microbial technology. The *Rummeliibacillus* sp., named *Rummeliibacillus* JY-2-4R, was deposited on February 11, 2026, at the Guangdong Provincial Microbial Culture Collection Center, located at 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou, with accession number GDMCC No: 67853. This invention applies the strain to the preparation of preparations antagonistic to *Actinidia chinensis* black spot, *Actinidia chinensis* soft rot, *Actinidia arvensis* white rot, *Actinidia arvensis* soft rot, or *Actinidia arvensis* root rot. The *Rummeliibacillus* JY-2-4R of this invention is a potential biological agent possessing stress resistance, growth promotion, and broad-spectrum antibacterial activity, with broad development potential and good application prospects, addressing the problem of a lack of functional microorganisms for the biological control of kiwifruit and *Actinidia arvensis* diseases.

This invention belongs to the field of biotechnology-assisted breeding and discloses a SNP marker for identifying resistance to bacterial soft rot in cucumber and its application. The SNP molecular marker of this invention is located at physical positions 13,925,944 bp on cucumber chromosome 2. Cucumber materials with a base of G at this site are resistant to bacterial soft rot, while cucumber materials with a base of A at this site are susceptible to bacterial soft rot. Using the SNP marker provided by this invention, the resistance of cucumber plants to bacterial soft rot can be rapidly and accurately identified during the seedling stage, enabling high-throughput screening of a large number of breeding materials. This method has advantages such as high detection efficiency and no limitation by environmental conditions, significantly improving the selection efficiency in disease-resistant breeding, shortening the breeding cycle, and providing an effective tool for marker-assisted selection breeding of cucumbers resistant to bacterial soft rot.

This invention proposes a copper-manganese layered bimetallic hydroxide nanomaterial, its preparation method, and its application, belonging to the field of agricultural pest control technology. The nanomaterial has an average hydrated particle size of 100-2000 nm, a negative zeta potential, and a copper-to-manganese molar ratio of 2.5-3.5:1. The nanomaterial exhibits excellent control effects against soft rot in calla lily plants, effectively inhibiting pathogen infection and disease spread. It possesses the advantages of low effective concentration and good persistence, providing a novel and highly efficient nano-antibacterial material for the green control of bacterial soft rot in plants. It also provides a theoretical basis and technical support for the application of layered bimetallic hydroxides in agricultural plant protection.

The application discloses a method for improving the soft rot resistance of kiwifruit, and specifically relates to a method for irradiating the picked kiwifruit with blue light. 2 The wavelength of the blue light is 450 nm, and the light intensity of the blue light is 75 umol / m 2 / s or more. The method can effectively improve the soft rot resistance of the kiwifruit, reduce the incidence of soft rot of the kiwifruit during storage, prolong the storage time of the kiwifruit, and is simple in operation and convenient for large-scale use, thereby effectively solving the problems in the prior art.

This invention discloses a method derived from Bacillus ( Bacillus The population quenching lactonease YtnP gene of sp.) GB-1, the nucleotide sequence of which is shown in SEQ ID NO:1, was introduced into the soft rot pathogen via a two-parent hybridization method. Heterologous overexpression of this gene significantly inhibited biofilm formation, motility, and host pathogenicity of the soft rot pathogen. It was also transferred into Arabidopsis thaliana via Agrobacterium-mediated transformation and overexpressed in Arabidopsis. ytnP Genes can significantly enhance plant resistance to soft rot, and this invention provides new resources and technical approaches for the prevention and control of soft rot.

This invention belongs to the field of gene technology and discloses a bacterial strain antagonistic to konjac soft rot and its application. The *Pseudomonas pallens* strain B07 of this invention exhibits significant antagonistic activity against the pathogen causing konjac soft rot, with an inhibition zone diameter of 1.05±0.16 cm. In pot experiments, it reduced the incidence of konjac soft rot by 33.34% and the disease index by 22.22%. Simultaneously, this strain possesses plant growth-promoting characteristics such as phosphorus solubilization, siderophore production, and nitrogen fixation. It also shows broad-spectrum antagonistic activity against eight plant pathogenic fungi, including konjac white mold and *Amorphophallus bulbifer* leaf spot, with inhibition rates ranging from 44.87% to 60.03%. Whole-genome analysis shows that its genome contains 14 secondary metabolite biosynthesis gene clusters, providing the molecular basis for its antibacterial activity. This strain can be applied directly or prepared as a biocontrol agent for the prevention and control of konjac soft rot. It is environmentally friendly, residue-free, and possesses both disease prevention and growth-promoting effects, making it of significant industrial value in the green control of konjac diseases.

The application belongs to the technical field of biological disease prevention and treatment, and particularly relates to application of wormwood essential oil in prevention and treatment of soft rot of sweet potato. The soft rot of sweet potato is caused by Rhizopus stolonifer. The wormwood essential oil is prepared by using a water vapor distillation method. In view of the broad-spectrum bacteriostasis of the wormwood essential oil, the wormwood essential oil is innovatively used in the prevention and treatment experiment of the soft rot of sweet potato. The preliminary experimental results show that the wormwood essential oil has a good bacteriostatic effect on the pathogenic bacteria Rhizopus stolonifer causing the soft rot of sweet potato. In view of the advantages of the wormwood essential oil such as safety, no pollution and natural source, based on the results, better technical reference and practical significance can be provided for the harvesting and preservation of sweet potato and the postharvest preservation of other crops.