127 results about "Soft rot disease" patented technology

The invention relates to the technical field of microorganisms and bio-organic fertilizers, and particularly discloses a bio-organic fertilizer for preventing and treating rhizome diseases of horseradish and application of the bio-organic fertilizer, the bio-organic fertilizer comprises the following components: component A: a bacillus velezensis microbial inoculum with the preservation number of CCTCC NO: M 2022923 and a trichoderma yunnanense microbial inoculum with the preservation number of CCTCC NO: M 2025645; the component B is prepared from soybean meal, vinegar residues, charcoal, wollastonite powder and allicin. The biological organic fertilizer disclosed by the invention can be used for increasing the content of growth hormones such as salicylic acid (SA), jasmonic acid (JA) and ethylene (ET) and phytoalexin as well as the activity of chitinase and beta-1, 3-glucanase of a wasabi root system, and has a relatively good prevention and treatment effect on a black heart disease caused by infection of phoma wabi Yokog and a soft rot disease caused by infection of pectobacterium carotovorum subsp. Carotovorum (Pcc). The biological organic fertilizer disclosed by the invention has a relatively good prevention and treatment effect on the soft rot disease caused by infection of the phoma wabi Yokog and a relatively good prevention and treatment effect on the soft rot disease caused by infection of the phoma wabi subsp. Carotovorum (Pcc).

The invention discloses an application of IbWRKY26 protein and a coding gene thereof in regulation and control of sweet potato soft rot resistance, and belongs to the technical field of plant genetic engineering. The invention provides an application of the IbWRKY26 protein in any one of the following items: (1) an application in regulation and control of sweet potato soft rot; (2) application in preparation of products for regulating and controlling sweet potato soft rot; (3) application in soft rot resistant sweet potato breeding; (4) application in cultivation of soft rot resistant sweet potatoes; the amino acid sequence of the IbWRKY26 protein is as shown in SEQ ID NO. 2. Experiments prove that compared with a wild type sweet potato, the soft rot disease resistance of the overexpressed IbWRKY26 gene is remarkably improved. The IbWRKY26 protein and the coding gene thereof provided by the invention have important theoretical significance and application value in the research on the soft rot resistance of sweet potatoes.

The invention discloses application of Indel marker dosage typing in detection of sweet potato soft rot resistance, and belongs to the technical field of plant genetic engineering. The Indel molecular marker related to the sweet potato soft rot resistance is obtained through screening, the nucleotide sequence of the Indel molecular marker is shown as SEQ ID NO.2, and an 8bp sequence is inserted into or deleted from the 161st site of the sequence shown as SEQ ID NO.2. Experiments find that target bands of amplification products are obtained through amplification of the Indel molecular marker and electrophoresis, dosage values of two genotypes are obtained according to gray values of the dyed electrophoretic bands, and results show that the insertion type Indel molecular marker is positively correlated with the sweet potato soft rot resistance. The method is of great significance to the dose effect research and molecular assisted breeding of sweet potato gene functions.

The invention belongs to the technical field of microbial agents, and particularly relates to a preservative and fresh-keeping microbial composition as well as a microbial agent and application thereof. The microbial composition disclosed by the invention comprises bacillus subtilis LS and bacillus velezensis LX, and in the microbial composition, the concentration ratio of the bacillus subtilis LS to the bacillus velezensis LX is (0.1-1): (0.1-1); the preservation number of the bacillus subtilis LS is CGMCC (China General Microbiological Culture Collection Center) NO.30953; the preservation number of the bacillus velezensis LX is CGMCC (China General Microbiological Culture Collection Center) NO. 30954. In the microbial composition provided by the invention, the bacillus subtilis LS and the bacillus velezensis LX can generate a synergistic effect and can be used for preventing and treating the soft rot of the kiwi fruits, and a theoretical basis and technical guidance are provided for biological prevention and treatment and storage and fresh-keeping of the soft rot of the kiwi fruits.

The invention discloses Delftia tsuhuensis and an application of the Delftia tsuhuensis in prevention and treatment of plant diseases. The Delftia tsuhuensis CDJ2 is preserved in the Guangdong Microbial Culture Collection Center on October 18, 2024, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) NO: 65290. The strain has a good prevention and treatment effect on citrus canker, and the prevention and treatment effect can reach 84%. Meanwhile, the strain can also effectively prevent and treat soft rot of taro, bacterial basal rot of rice, bacterial wilt of crops, kiwifruit canker, angular leaf spot of mango, black rot of brassicaceous vegetables, tomato scab, gummosis of sugarcane, bacterial wilt of walnuts and bacterial leaf spot of plums. Wide application prospects are realized in the aspects of preventing and treating diseases of crops and commercial crops and promoting healthy growth of plants.

The invention discloses bacillus tequilensis P61 and application thereof in prevention and treatment of kiwi fruit soft rot, and belongs to the field of biological prevention and treatment. The bacillus tequilensis P61 provided by the invention is preserved in the China Center for Type Culture Collection on September 5, 2024, and the preservation number of the bacillus tequilensis P61 is CCTCC (China Center for Type Culture Collection) NO: M 20241916. According to the invention, the lipopeptide antibacterial substance is prepared according to the bacillus tequilensis P61. Experimental results show that the bacillus tequilensis P61 and lipopeptide antibacterial substances have an outstanding prevention and treatment effect on the soft rot of the kiwi fruits, have a remarkable bacteriostasis effect on pathogenic bacteria of the soft rot of the kiwi fruits such as botryosphaeria dothidea, alternaria alternata and phomopsis, remarkably reduce the damage rate of the kiwi fruits, are green and safe, are harmless to people and livestock, and are worthy of popularization and application. No pollution is caused to the environment. The invention provides a new biocontrol strain for biological prevention and control of kiwi fruit diseases, and has a wide application prospect.

The invention discloses an application of a sweet potato transcription factor IbERF114 and a coding gene thereof in regulation and control of sweet potato soft rot resistance, and belongs to the technical field of gene engineering. Experiments prove that compared with a wild sweet potato plant, a transgenic sweet potato plant over-expressing the IbERF114 gene and an interference sweet potato plant interfering the IbERF114 gene have the advantages that the resistance of the transgenic sweet potato plant to the soft rot is obviously enhanced, the resistance of the interference sweet potato plant is obviously reduced, and the IbERF114 protein and the coding gene thereof play a key regulation role in the soft rot resistance of the sweet potato. The discovery of functions of the protein and the coding gene thereof has important production values for improving the disease resistance of sweet potatoes, creating excellent disease-resistant parent elements and cultivating high-resistance varieties.

The present invention provides a system for differential delivery of live BALO. The system comprises: a carrier comprising a polysaccharide hydrocolloid; and BALO immobilized within the carrier wherein the BALO is present in a physiological state selected from the group consisting of leech plastid, leech cyst and AP, which is present at a cell density of at least about 1.0 * 108 PFU / g carrier wherein the polysaccharide hydrocolloid is in the form of a wet or dry gel, and wherein the carrier preserves the viability and lysolytic efficacy of the immobilized BALO. Further provided are methods for making the systems for differential delivery of live BALO, as well as methods for using the same, e.g., for treating phytopathogenic bacterial diseases in plants or crops.

The invention discloses a method for regulating and controlling soft rot resistance of sweet potatoes by utilizing an IbHD2C gene and application of the method, and belongs to the technical field of gene engineering. The invention discloses an application of an IbHD2C gene (the nucleotide sequence is shown as SEQ ID NO.3) and an encoding protein (the amino acid sequence is shown as SEQ ID NO.4) thereof in regulation and control of soft rot resistance. Experiments prove that the resistance of sweet potatoes to soft rot can be remarkably improved by interfering the IbHD2C gene, and the resistance is reduced by overexpressing the gene. The invention provides a new target for disease-resistant breeding of sweet potatoes, solves the problems of dependence on chemical agents and lack of disease-resistant varieties in the prior art, and has the advantages of high efficiency, environmental protection and sustainability.

The invention relates to the technical field of biology, in particular to pure bacillus simplex ZF535 capable of producing exopolysaccharides and application of the pure bacillus simplex ZF535. The preservation registration number of the simple pure bacillus ZF535 disclosed by the invention in the China General Microbiological Culture Collection Center (CGMCC) is CGMCC No: 34733. Pot experiment results show that leaf spraying or root irrigation treatment of exopolysaccharides of the strain ZF535 has a remarkable induced disease-resistant effect on cucumber angular spots, and the prevention effects are 56.59% and 60.43% respectively; the strain ZF535 exopolysaccharide has a remarkable induced disease-resistant effect on the cucumber soft rot disease through leaf spraying or root irrigation treatment, and the prevention effects are 54.35% and 65.11% respectively. In conclusion, the strain ZF535 is a simple pure bacillus with potential biocontrol application potential for inducing plant disease resistance, and has a better biocontrol effect.

The cultivation method comprises the steps that the macadamia nut forest with the tree age larger than or equal to 5 years is selected, PVC permeable pipes with water seepage holes are laid at the bottoms of ditches, and the ditches are covered with gravel layers; ridging is conducted along the dead furrows, and a lower layer of fallen leaf crushed matter mixed decomposed farmyard manure, a middle layer of acid red soil and an upper layer of special konjak matrix containing functional microorganisms are constructed on ridge bodies in a layered mode; performing cold shock treatment on the seed taros, soaking the seed taros in chitosan-nano-selenium composite liquid, and coating the seed taros with nut oleoresin; the height of the sunshade net is dynamically regulated and controlled through an intelligent system to maintain the shading rate to be 60%-70%, and automatic drip irrigation is carried out based on a soil humidity sensor; harvesting and retaining rhizomes after konjak seedlings fall, removing konjak plants before nut fruit drop, and collecting dropped fruits by utilizing dead furrows. According to the method, the problems of water and light accurate regulation and control, root competition and harvesting conflicts are solved, waste recycling is achieved, the soft rot rate is decreased, the fruit drop loss rate is greatly decreased, and the comprehensive output value is remarkably increased.

The invention discloses a derivative containing chiral thioether and sulfone units as well as a preparation method and application of the derivative. The derivative containing the chiral thioether and the sulfone unit is applied to preparation of medicaments for preventing and treating rice bacterial leaf blight, rice bacterial leaf streak, citrus canker, tobacco bacterial wilt, muskmelon bacterial leaf blight, tomato bacterial wilt, watermelon fruit rot, Chinese cabbage soft rot, peach tree shot hole disease and cabbage black rot fungus. The invention further discloses application of the derivative containing the chiral thioether and sulfone unit to preparation of medicaments for preventing and treating rice bacterial leaf blight, rice bacterial leaf streak, citrus canker, tobacco bacterial wilt, muskmelon bacterial leaf blight, tomato bacterial wilt, watermelon fruit rot and cabbage black rot. The derivative containing chiral thioether and sulfone units has the characteristics of broad bactericidal spectrum and high activity, has excellent prevention and treatment effects on bacterial diseases, and is simple and feasible in preparation steps and low in production cost.

The present invention discloses the application of isoquinoline-3-carboxylic acid in preventing and controlling plant bacterial diseases. The isoquinoline-3-carboxylic acid provided by the present invention has an obvious inhibitory effect on plant bacterial diseases, and the plant bacterial diseases are Ralstonia solanacearum, Acidovorax avenae subsp. Citrulli, Pseudomonas syringae pv. actinidiae, Pectobacterium carotovorum subsp. carotovorum, Xanthomonas oryzae pv. oryzae, Xanthomonas oryzae pv. oryzicola, Alternaria brassicae, Xanthomonas citri subsp. citri, Pectobacterium carotovorum subsp. carotovorum, Xanthomonas fragariae, Pseudomonas syringae pv. lachrymans, Dickeya dadantii, Xanthomonas axonopodis pv. mangiferaeindicae. The isoquinoline-3-carboxylic acid of the present invention has protective and therapeutic effects on Acidovorax avenae subsp. Citrulli.

The present invention discloses the application of coumarin-3-carboxylic acid in preventing and treating plant bacterial diseases. The coumarin-3-carboxylic acid provided by the present invention has a significant inhibitory effect on plant bacterial diseases. The plant bacterial diseases are oryzae bacterial leaf blight, oryzae bacterial leaf streak, citrus canker, cantaloupe bacterial fruit spot, tomato bacterial wilt, strawberry bacterial angular leaf spot, cabbage black rot, cabbage soft rot, potato soft rot, mango bacterial angular leaf spot, cassava bacterial wilt, cucumber bacterial angular leaf spot, kiwifruit canker and banana bacterial soft rot. The coumarin-3-carboxylic acid provided by the present invention has protective and therapeutic effects on melon bacterial fruit spot.

The invention provides a polygonatum kingianum root endophytic fungus Princasa crispa DHJ068 and application thereof, and relates to the technical field of crop disease control. The polygonatum kingianum root endophytic fungus Princephala crispa DHJ068 is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M 2025748, and a fermentation liquor crude extract of the Princephala crispa DHJ068 can be applied to prevention and treatment of crop diseases. The invention overcomes the defects in the prior art, and the fermentation liquor crude extract of the polygonatum kingianum root endophytic fungus Prringtonia crispa DHJ068 can effectively inhibit the growth of Chinese cabbage soft rot bacteria and cucumber bacterial angular leaf spot bacteria, thereby achieving the purpose of certain biological control.

The invention relates to the technical field of biology, in particular to application of protein IbNFYA3 in regulation and control of soft rot resistance of sweet potatoes. It is found for the first time that the content of jasmonic acid and the content of aspartic protease can be remarkably increased through overexpression of the NFYA gene in a plant, so that soft rot bacteria can be effectively resisted, specifically, the invention provides an IbNFYA3 gene for coding an NFYA transcription factor (protein IbNFYA3), the gene is introduced into sweet potatoes to obtain a transgenic sweet potato plant with the overexpression of the IbNFYA3 gene, and the transgenic sweet potato plant has the advantages that the content of jasmonic acid and the content of aspartic protease can be effectively resisted; soft rot resistance identification is carried out on the transgenic sweet potato blocks, and it is found that compared with wild sweet potatoes, the soft rot resistance of the transgenic sweet potato blocks is obviously enhanced. Therefore, the IbNFYA3 protein and the coding gene thereof can regulate and control the soft rot resistance of plants, and can be used for cultivating transgenic plants with high disease resistance.

A new and distinct cultivar of Zantedeschia plant named ‘DO21PW104’, characterized by its upright and broadly arching plant habit; high production of cut flowering stems; large white-colored spathes with light purple-colored lower surfaces and strong and erect scapes; good cut flower postproduction longevity; and relative tolerance to Soft Rot (Erwinia carotovora).

The invention relates to a special microbial agent fertilizer for konjak, and aims to break through the industrial bottlenecks of high incidence of soft rot, damage caused by waterlogging stress and glucomannan synthesis obstacles in konjak cultivation. According to the core technical scheme, the activity of soft rot fungus pectinase is inhibited in a targeted mode through clostridium butyricum, and efficient polymerization of gulcomannan molecular chains is driven in cooperation with a hydroxyproline precursor provided by fish meal; a sulfonic acid group of biochemical potassium fulvate is used for blocking waterlogging free radical poison, and a brown sugar-trehalose carbon source ratio is optimized to stabilize cell osmotic pressure in an expansion period; and a supplementary application mechanism based on a precipitation threshold (greater than or equal to 10mm) and a copper preparation application isolation regulation are established in a matched manner. According to the technology, the three-effect synergy that the soft rot prevention and control rate is larger than 90%, the glucomannan viscosity improvement rate is larger than 70% and the waterlogging survival rate is larger than 85% is achieved, and the core restriction of high-yield and high-quality production of the konjak is systematically solved.

The invention provides an antibacterial peptide, application of the antibacterial peptide and an antibacterial drug and a preservative prepared from the antibacterial peptide, and belongs to the technical field of biology. The antibacterial peptide is an amino acid sequence as shown in SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3 or an amino acid sequence which has more than 95% of homology with the amino acid sequence as shown in SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3. The three antibacterial peptides have broad-spectrum antibacterial effects, have obvious inhibition effects on gram-positive bacteria, gram-negative bacteria and fungi, and can be prepared into antibacterial drugs for use. And simultaneously. The antibacterial film agent prepared from the antibacterial peptide can be used as an antibacterial preservative, and can delay proliferation of putrefying bacteria in food or medicines, so that the shelf life of the food or medicines is prolonged, for example, the antibacterial peptide can be used for preventing and treating soft rot of kiwi fruits. The antibacterial peptide has a good application prospect in the fields of food, medicine and the like.

The invention discloses a high-performance antagonistic bacterium RQH1 and application of the high-performance antagonistic bacterium RQH1 in prevention and control of amorphophallus konjac soft rot. The high-performance antagonistic bacterium RQH1 is preserved in China Center for Type Culture Collection, the preservation number is CCTCC NO. M 2023719, the preservation address is Wuhan University, Wuhan, China, and the preservation time is May 10, 2023. According to the invention, a high-performance antagonistic bacterium RQH1 is obtained by directional screening in a healthy konjak plant body, and the strain not only shows broad-spectrum and high-efficiency bacteriostatic activity on various konjak soft rot pathogenic bacteria, but also obviously inhibits the pathogenic bacteria from growing and infecting; meanwhile, various growth promoting functions such as phosphorus dissolving, potassium dissolving and nitrogen fixing can be achieved, the soil nutrient condition can be improved, and konjak plants can be promoted to grow robustly. In addition, the composition and antibacterial mechanism of volatile metabolites of the RQH1 strain are systematically analyzed. Therefore, a high-value strain resource which is multifunctional, easy to culture and high in adaptability is provided for green prevention and control of the soft rot of the konjak, and various defects in the prior art are effectively overcome.

The invention belongs to the field of chemical medicine synthesis, and particularly relates to an acylhydrazone compound, a preparation method thereof and application of the acylhydrazone compound in camellia oleifera anthracnose and soft rot. The structure of the acylhydrazone compound is shown as a formula I, and the acylhydrazone compound is directly prepared from citronellal and formylhydrazine through a one-step reaction. The invention provides the acylhydrazone compound, the IC50 of the acylhydrazone compound on liver cancer can reach 7.0 mu M, and a new solution is provided for research and development of drugs for treating liver cancer. The EC50 (mg / L) of the strain can reach 1.65 when the strain is used in an oil-tea camellia anthracnose strain inhibition experiment, the EC50 (mg / L) of the strain can reach 1.38 when the strain is used in an oil-tea camellia soft rot strain inhibition experiment, and a new solution is provided for controlling oil-tea camellia anthracnose and soft rot. Meanwhile, the invention provides a synthesis method of citronellal and formylhydrazine through one-step reaction, and the synthesis method has the advantages of high yield, high raw material conversion rate, high selectivity and the like. And the formula I of the # imgabs0 # is shown in the specification.

This invention relates to the field of agricultural pest control, and particularly to a compound nanogel sustained-release agent, its preparation method, and its application. This invention provides a compound nanogel sustained-release agent comprising bromonitol, a synergist, a carrier material, and a cross-linking agent, wherein the synergist includes one or more of kasugamycin, amino oligosaccharides, thiazolium zinc, and copper hydroxide. This invention prepares a nanogel sustained-release agent by compounding bromonitol and the synergist, which has significant preventive effects against plant bacterial diseases such as bacterial wilt, bacterial angular leaf spot, canker, and soft rot. Furthermore, the compound nanogel sustained-release agent of this invention has small particle size, stable physicochemical properties under cold and heat, good dispersibility in water, no particle agglomeration, no particle size increase, a decomposition rate of less than 5%, a long-lasting effect, good safety, is not prone to inducing drug resistance, requires a small dosage, and simultaneously increases yield and efficiency.

The invention belongs to the technical field of biological prevention and control of microorganisms and plant diseases, and particularly relates to a Penicillium griseofulvum strain antagonizing soft rot of Pinellia ternate and application of the Penicillium griseofulvum strain in prevention and control of plant diseases. The Penicillium griseofulvum AFP-25 strain provided by the invention is a strain of pinellia ternata soil rhizosphere microorganism, is safe to human, livestock and crops, and is friendly to the environment. The Penicillium griseofulvum has a very strong antagonistic effect on soft rot of pinellia ternate, is easy to colonize in pinellia ternate plants and surroundings thereof, utilizes Penicillium griseofulvum to biocontrol soft rot of pinellia ternate, is a stable Penicillium griseofulvum biocontrol mode, is low in cost, and can better solve the problem that large-area outbreak of soft rot of pinellia ternate cannot be controlled in agricultural production. Meanwhile, the penicillium griseofulvum can also effectively inhibit generation and spreading of other plant fungal diseases such as root rot, so that the penicillium griseofulvum has good practical application value.

The invention belongs to the technical field of molecular biology, and particularly relates to a molecular marker related to soft rot resistance of zantedeschia aethiopica and application of the molecular marker. The molecular marker is located at the 68676823rd site of a zantedeschia No.4 chromosome and can be used for identifying whether a zantedeschia variety has resistance to soft rot or not. The identification of the common callalily soft rot resistance is enriched and simplified, and the breeding period of a soft rot resistance material is shortened. And a technical support is provided for germplasm resource utilization and molecular breeding of the calla lily.

The invention discloses a SNP molecular marker for identifying resistance of cucumber to bacterial stem rot and its application, belonging to the field of biotechnology-assisted breeding technology. The site of the SNP marker is at the 10,867,465 bp of the physical position of cucumber chromosome 6, the base of the site is A or G, the cucumber material with the base of the site A is a cucumber material resistant to bacterial stem rot, and the cucumber material with the base of the site G is a cucumber material susceptible to bacterial stem rot. The invention not only lays a foundation for the precise positioning and molecular cloning of the cucumber bacterial stem rot resistance gene BSSR, but also provides an efficient way for the use of molecular markers to assist in the breeding of new cucumber varieties resistant to cucumber bacterial stem rot.

The invention belongs to the technical field of gene engineering, and particularly relates to application of a PtZFP20 gene in regulating soft rot resistance of pinellia ternate. The invention discovers that PtZFP20 is closely related to the soft rot resistance of pinellia ternate for the first time, and the soft rot resistance of pinellia ternate can be improved by increasing the expression quantity of PtZFP20. The PtZFP20 gene provides a powerful technical support for cultivating a new disease-resistant variety of pinellia ternate, and can be widely applied to breeding and utilization of the disease-resistant variety of pinellia ternate.

The invention discloses a construction method and application of rhizosphere synthetic flora for preventing and controlling amorphophallus konjac soft rot, and belongs to the technical field of microorganisms. According to the method, three kinds of konjac rhizosphere soil with different intercropping modes are collected, the structure and composition differences of the konjac rhizosphere soil are analyzed through an amplicon high-throughput sequencing technology, and meanwhile culturable microorganisms are separated; network core hubs are analyzed and screened based on a co-occurrence network, dominant species are analyzed and screened based on specificity-occupancy, flora of synthetic microbiome is screened and constructed in different intercropping konjac rhizosphere soil, the constructed synthetic flora can remarkably reduce the morbidity of soft rot of potted konjac, growth of the konjac is promoted to a certain extent, and the yield of the potted konjac is increased. The quality of konjak tubers is improved. In addition, the synthetic flora provided by the invention can improve the micro-ecology of konjac rhizosphere, recruit more beneficial microorganisms and synergistically inhibit the occurrence of soft rot of konjac.

The present invention discloses a chalcone derivative containing a benzoxazole structure, a preparation method thereof, and an application thereof, and belongs to the field of chemical technology. The present invention uses 1-(4-(3-bromopropyloxy)phenyl)-3-(substituted aromatic)-2-propylene-1-one or 1-(4-(4-bromobutyloxy)phenyl)-3-(substituted aromatic)-2-propylene-1-one to undergo an etherification reaction with benzo[d]oxazole-2-thiol to generate a chalcone derivative containing a benzoxazole structure, the structural formula of which is as follows, wherein n is 3 or 4, and R is any one of a phenyl, a substituted phenyl, a thienyl, or a furyl group. Experimental testing has shown that the derivative has good inhibitory activity against plant bacteria such as rice bacterial blight, melon wilt, and potato soft rot, and can be used as a potential agent for inhibiting plant bacteria.

The invention provides a microbial agent and application thereof, and belongs to the technical field of biology, the microbial agent comprises the following components: a component A: Penicillium restrictum and Penicillium verruculosum, a component B: Penicillium verruculosum, a component C: Penicillium verruculosum, and a component D: Penicillium verruculosum; the component B comprises sodium alginate, fulvic acid, a panax japonicus extract, a polygonatum odoratum extract and corn starch; wherein the addition amount of the penicillium limited accounts for 1%-3% of the total weight of the microbial agent, the addition amount of the penicillium verrucosum accounts for 1%-5% of the total weight of the microbial agent, and the balance is the component B. The microbial agent provided by the invention can be used for effectively preventing and treating the Chinese cabbage soft rot caused by the pectobacterium carotovorum (Pectobacterium carotovorum). The microbial agent provided by the invention can be used for effectively preventing and treating the Chinese cabbage soft rot caused by the pectobacterium carotovorum.

The mass ratio of quinolinic acid to clindamycin is (1-3): (7-9), after the clindamycin and the quinolinic acid are used in a combined mode, a synergistic effect is achieved in the aspect of prevention and treatment of the Chinese cabbage soft rot, and compared with the concentration required for bacteriostasis of single clindamycin or single quinolinic acid, the concentration required for bacteriostasis of the clindamycin and the quinolinic acid is reduced, so that the effect of preventing and treating the Chinese cabbage soft rot is improved. After compounding, the use amount of the two is greatly reduced, and the composition has a remarkable control effect on the soft rot of the Chinese cabbage.