Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bacillus simplex RJGP41 and application thereof

A pure bacillus, simple technology, applied in the field of simple pure bacillus, can solve the problems of air, water, soil and food pollution, lack of chemical agents, etc.

Inactive Publication Date: 2010-12-29
NANJING HOUJI BIO TECH
View PDF0 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is a lack of effective chemical agents for bacterial diseases of crops, including Chinese cabbage soft rot, and the extensive use of chemical pesticides has not only caused air, water, soil and food pollution, but more importantly, through the directional selection of chemical pesticides, pathogenic bacteria have been produced. Drug resistance, people are eager to find new pesticides with low toxicity, high efficiency and environmental friendliness in the biological field

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacillus simplex RJGP41 and application thereof
  • Bacillus simplex RJGP41 and application thereof
  • Bacillus simplex RJGP41 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Use the five-point sampling method, remove the topsoil with a small shovel, take dozens of grams of soil samples at a distance of 5-25mm, put them into a sampling bag, and mark the sampling time, location, and vegetation. Weigh 5g of soil sample, put it into 45ml of physiological saline, shake it on a shaker for 30 minutes, and let it stand for clarification. Dilute the supernatant with sterile water for 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 Five concentration gradients, 80°C water bath for 10min. Aspirate 200μl coated LB plates and culture overnight at 37°C. After colonies grow on the plate, single colonies are picked, streaked and purified, and numbered and stored, and screened to obtain simple pure Bacillus (Bacillus simplex) RJGP41 CGMCCNo.3381.

Embodiment 2

[0020] The simple pure bacillus (Bacillus simplex) RJGP41CGMCC No.3381 of embodiment 1 is carried out morphological characteristic observation and physiological and biochemical characteristic identification, the result shows, this bacterium is Gram-positive, rod-shaped, produces spore (as figure 1 shown). Can grow at 4°C, but not at 50°C, cannot tolerate the culture of pH 5.7 and 7% NaCl, contact enzyme test, starch hydrolysis test, citrate utilization test, nitrate reduction test, glucose fermentation and casein The tests were all positive, and the V-P test, anaerobic growth test, and propionate utilization test were all negative.

Embodiment 3

[0022] The simple pure bacillus (Bacillus simplex) RJGP41CGMCC No.3381 of Example 1 was inoculated in liquid LB medium, 180 rpm, 37 ° C shaking culture for 10 hours, centrifuged to collect the bacteria, after suspension, add lysozyme and SDS to break the wall, use phenol - Chloroform method to extract genomic DNA. Using the extracted total DNA as a template, the 16S rDNA gene of the strain was amplified by PCR with forward primer PRB1601 (5'-GGATCCTAATACATGCAAGTCGAGCGG-3') and reverse primer PRB1602 (5'-GGATCCACGTATTACCGCGGCTGCTGGC-3'). The PCR conditions are: 95°C, 4min; 94°C, 1min; 50°C, 1min; 72°C, 2min; 34cycle; 72°C, 10min, stored at 4°C. After the PCR product was recovered, it was sequenced, and its length was confirmed to be 481bp. The sequencing result was shown in the sequence table, and the similarity with Bacillus simplexFJ455076.1 in GenBank was 94%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to bacillus simplex RJGP41 which was collected in China General Microbiological Culture Collection Center (CGMCC) in November 2, 2009, with collection number of CGMCC No.3381. The bacillus simplex RJGP41 with collection number of CGMCC No.3381 is biocontrol bacillus isolated from surrounding oil of dandelion roots in Guo'an village, Jiangdang town, Shigatse city, Tibet, can grow at 4 DEG C, grows well under low temperature, is beneficial to giving play to growth-promoting and biocontrol effects, has rate of controlling soft rot of Chinese cabbage as high as 83% and simultaneously increases the output of the Chinese cabbage by 50%.

Description

technical field [0001] The invention relates to a simple pure bacillus, specifically, a low-temperature suitable pure bacillus simplex (Bacillus simplex) RJGP41 isolated from Tibet with the functions of promoting growth and preventing diseases and its role in the prevention and treatment of Chinese cabbage soft rot application. Background technique [0002] Soft rot of Chinese cabbage is one of the three major diseases of Chinese cabbage. It is caused by Erwinia. This bacterium is ubiquitous in the soil and is spread by rainwater and irrigation water. The pathogen can infect the disease as long as it comes into contact with the cabbage wound. If it is not prevented and controlled in time, it will directly affect the yield and quality of Chinese cabbage. At present, there is a lack of effective chemical agents for bacterial diseases of crops, including Chinese cabbage soft rot, and the extensive use of chemical pesticides has not only caused air, water, soil and food polluti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20A01N63/00A01P21/00A01P1/00C12R1/07
Inventor 高学文薛鹏琦刘芳伍辉军沈波
Owner NANJING HOUJI BIO TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com