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Bacillusamyloliquefaciens subsp Lh-1 and application thereof

A technology for dissolving starch spores and bacilli, applied in the application, bacteria, fungicides and other directions, to achieve the effect of simple production process, significant control effect, and reduced dosage

Inactive Publication Date: 2015-04-22
AGRI BIOTECH RES CENT OF SHANXI PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is no combination of Bacillus amyloliquefaciens and chitosan oligosaccharides for the control of cotton verticillium wilt and other Chinese cabbage soft rot, peach tree rot, apple rot, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Bacillus amyloliquefaciens of the present invention ( Bacillus amylolique faciens subsp ) Isolation and Identification of LH-1

[0018] 1. Sample collection

[0019] Sampling 100g from the soil surface to 15cm from the experimental field of Shanxi Academy of Agricultural Sciences, dried at 60°C for 24 hours, and took 1g for separation.

[0020] 2. Isolation and resistance screening of strains

[0021] (1) Strain dilution: Weigh 1g and dissolve it in a triangular flask filled with 100ml sterilized distilled water to make a soil suspension, and then gradiently dilute to 10 -9 soil suspension.

[0022] (2) Separation and screening of strains: absorb 50ul of soil suspension and 50ul of cotton Verticillium dahliae bacteria cultured in liquid for 3 days, add it to the cooled PDA medium, spread evenly, place it in a 30°C incubator and culture it upside down for 24h, observe And select antagonistic colonies (colonies with large inhibition zone);

[0023] (3) Purifica...

Embodiment 2

[0031] Example 2 Determination of Inhibition Spectrum of Bacillus amyloliquefaciens Lh-1

[0032] Cotton Verticillium dahliae ( Verticillium dahliae ), Chinese cabbage soft rot ( Erwinia carotovora ), Ralstonia solanacearum ( Ralstonia solanacearum ), peach tree rot fungus ( Valsa leucostoma ), apple rot fungus ( Valsa mali Miyabe et Yamada ), Phytophthora capsici ( Phytophthora capsici ), Botrytis cinerea ( Phytophthora infestans ) as an indicator bacterium to determine the antibacterial effect of Bacillus amyloliquefaciens Lh-1. The specific method is as follows: under aseptic conditions, culture 48h, dilute 10 5 Double 50ul Bacillus amyloliquefaciens Lh-1 bacteria solution was spread evenly on the PDA plate medium, and cultured upside down in a 31°C incubator for 48h. After the colonies were inactivated by fumigation with chloroform for 20 minutes, a layer of soft agar containing indicator bacteria was used to spread a thin layer on the surface of the colonie...

Embodiment 3

[0037] Bacillus amyloliquefaciens ( Bacillus amylolique faciens subsp ) Preparation of Lh-1 probiotics

[0038] (1) Streak inoculate the Lh-1 strain stored in the -80°C refrigerator on the PDA solid slant medium, and cultivate it at 31°C for 48 hours to activate it. The activated strains were inoculated into PDA liquid medium, cultured with shaking at 31°C for 24 hours, and the bacterial liquid was used as the seed liquid for later use; (2) The raw materials and weight percentages for expanded fermentation production were as follows: corn flour 2%, bean cake 0.5%, 0.5% fish meal, 1% brown sugar, 0.1% sodium chloride, and the rest is water. Dissolve the raw materials in water according to the ratio and add them to the fermenter, sterilize with high-pressure steam, and inoculate the seed liquid obtained in step (1) into the fermenter. The inoculum amount accounts for 2-5% of the total volume of raw materials, preferably 3%, the stirring speed is 180r / min, 31°C, pH6.8, the relat...

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PUM

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Abstract

The invention relates to the field of microbial control, in particular to bacillusamyloliquefaciens subsp Lh-1 and application thereof. With a preservation number of CGMCC No.8548, the bacillusamyloliquefaciens subsp Lh-1 is applied in control of Verticillium dahliae, erwinia carotovora, ralstonia solanacearum, valsa leucostoma, Valsa Mali Miyabe et Yamada, Phytophthora capsici and phytophthora infestans. According to the invention, the bacillusamyloliquefaciens subsp Lh-1 and pesticide and chitosan oligosaccharide with dual effects of fertilizer and pesticide are combined into an Lh-1 microecological agent, the viable count is small, the dosage is small, the production process is simple, the cost is low, and the Lh-1 microecological agent has the dual functions of disease prevention and growth promotion. Nursery test proves that reduce the disease incidence of crop is reduced, especially for control of Verticillium dahliae, the disease incidence drops to 6%, and the control effect is significant. In addition, the Lh-1 microecological agent is a biological strain preparation, is completely free of a series of problems caused by use of chemical pesticides, thus being conducive to pollution-free production of crops, and farmers can have no need for pesticide or reduce the dosage of chemical pesticide, thus greatly saving the expenditure.

Description

technical field [0001] The invention relates to the field of microorganism control, in particular to a bacillus amyloliquefaciens Lh-1 and application thereof. Background technique [0002] It is well known that Bacillus amyloliquefaciens can produce a series of metabolites during its own growth process, and these metabolites can widely inhibit the activity of crop pathogenic fungi and bacteria, and the use of Bacillus amyloliquefaciens for effective biological control is safe. Efficient and pollution-free, the development potential is huge. The currently reported Bacillus amyloliquefaciens synthetic bacterial agent has a low number of live bacteria and inconvenient extraction of antibacterial substances, such as: CN100999667 discloses a soil improver for facility vegetable fields and its preparation method and application, emphasizing that as a soil improver, in the bacterial agent The ratio of strains and the ratio of solid bacterial agent carrier, but the number of live...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
CPCA01N63/00C12N1/205C12R2001/07
Inventor 黄静梁宏王长彪陈哲赵佳
Owner AGRI BIOTECH RES CENT OF SHANXI PROVINCE
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