162results about How to "Conducive to pollution-free production" patented technology

The invention relates to the field of microbial control, in particular to bacillusamyloliquefaciens subsp Lh-1 and application thereof. With a preservation number of CGMCC No.8548, the bacillusamyloliquefaciens subsp Lh-1 is applied in control of Verticillium dahliae, erwinia carotovora, ralstonia solanacearum, valsa leucostoma, Valsa Mali Miyabe et Yamada, Phytophthora capsici and phytophthora infestans. According to the invention, the bacillusamyloliquefaciens subsp Lh-1 and pesticide and chitosan oligosaccharide with dual effects of fertilizer and pesticide are combined into an Lh-1 microecological agent, the viable count is small, the dosage is small, the production process is simple, the cost is low, and the Lh-1 microecological agent has the dual functions of disease prevention and growth promotion. Nursery test proves that reduce the disease incidence of crop is reduced, especially for control of Verticillium dahliae, the disease incidence drops to 6%, and the control effect is significant. In addition, the Lh-1 microecological agent is a biological strain preparation, is completely free of a series of problems caused by use of chemical pesticides, thus being conducive to pollution-free production of crops, and farmers can have no need for pesticide or reduce the dosage of chemical pesticide, thus greatly saving the expenditure.

The invention belongs to the technical field of plant protection, and relates to a tobacco-black-shank-resisting and tobacco-bacterial-wilt-resisting bio-control strain Trb3. The bio-control strain is a bacillus subtilis. A method for preparing a microbial agent by using the strain Trb3 comprises the steps that: the strain Trb3 is subjected to oscillation culturing in an LB culture solution for 36-40h under a speed of 180rpm under temperature of 28 DEG C; the obtained material is centrifuged for 10min under a speed of 6000rpm; the material is diluted by using sterile water, such that the microbial agent is prepared, wherein a viable bacteria total concentration in the microbial agent finished product is 1*10<9>-1*10<10>u/ml. The Trb3 strain microbial agent is applied for root dipping before tobacco transplanting or for root irrigating during tobacco transplanting, and for root irrigating after tobacco transplanting and right before disease periods of tobacco black shank and tobacco bacterial wilt. In a greenhouse, a controlling effect of the Trb3 strain microbial agent against tobacco black shank reaches 72.3%, and a controlling effect of the Trb3 strain microbial agent against tobacco bacterial wilt reaches 65.7%. When the Trb3 strain microbial agent is applied in field, a controlling effect of the Trb3 strain microbial agent against black shank and tobacco bacterial wilt is 61.04%.

The invention relates to an antagonistic bacterium for preventing and killing off tobacco black shank and a microbial organic fertilizer thereof, belonging to a production technology of intensive agriculture. An antagonistic bacterium (paenibacillus polymyxa C-5) having obvious antagonistic effect on the tobacco black shank pathogenetic bacteria is separated and utilized to prepare into the microbial organic fertilizer with organic compost, wherein the microbial organic fertilizer respectively contains more than 1*108 C-5/g, the total-N content is 4-5 percent (more than 90 percent is organic nitrogen), the total nutrient content of nitrogen, phosphorus and potassium is 6-10 percent, and the content of organic matters is 30-35 percent. Proved by experiments, the microbial organic fertilizer enables the antagonistic bacterium to quickly breed and form a preponderant group in soil after being applied to the soil. The microbial organic fertilizer can greatly prevent the continuous cropping tobacco black shank if being applied to soil without continuous cropping obstacles for a long time.

The invention relates to antagonistic bacteria for preventing and eliminating greensickness of continuous cropping cotton and a microbial organic fertilizer thereof, belonging to a technology of agricultural intensification production. In the invention, two antagonistic bacteria (bacillus subtilis NJHJ-5 and bacillus subtilis NJDF-14) having obvious antagonistic actions on pathogenic bacteria of the cotton greensickness are separated, and the microbial organic fertilizer is prepared by utilizing the antagonistic bacteria and organic composts, wherein the microbial organic fertilizer respectively contains more than 1*10<9>/g of the NJHJ-5 and the NJDF-14, the total-nitrogen content is 4-5 percent (more than 90 percent of the content is organic nitrogen), total nitrogen-phosphorus-potassiumnutrients are 6-10 percent, and the content of organic matters is 30-35 percent. Proved by experiments, after being fertilized into soil, the microbial organic fertilizer enables the antagonistic bacteria to quickly reproduce and form a dominant group in the soil. After the microbial organic fertilizer is continuously fertilized for three seasons, the prevention and cure rate of the cotton greensickness in the soil the original morbidity of which is higher than 60 percent can reach 100 percent.

The invention relates to antagonistic bacteria NJL-14 for preventing and controlling continuous-cropping tobacco bacterial wilt, which belongs to intensive agricultural production technology. According to the invention, a microbial organic fertilizer is produced from two antagonistic bacteria (brevibacillus brevis NJL-25 and bacillus cereus NJL-14) which have a remarkable antagonistic function on pathogenic bacteria of the tobacco bacterial wilt and are obtained by separation, and organic fertilizers, wherein 1 gram of the microbial organic fertilizer contains more than 1*10<10> NJL-25 and 1*10<10> NJL-14, and has the total nitrogen content of 4 to 5 percent (organic nitrogen content is over 90 percent), the total nitrogen-phosphorus-potassium content of 6 to 10 percent and the content of organic matters of 30 to 35 percent. Tests prove that the microbial organic fertilizer, after being applied to soil, can fast multiply the antagonistic bacteria to form a dominant group in the soil, achieve the biological prevention and control rate of over 97.6 percent on the bacterial wilt in the soil with the tobacco bacterial wilt, and prevent the occurrence of the continuous-cropping tobacco bacterial wilt to a great extent after being applied for long to the soil without continuous cropping obstacles.

The invention relates to the field of microbial fertilizers, and particularly relates to a bacillus amyloliquefaciens strain T-5 for the prevention and treatment of tomato bacterial wilt and a microbial organic fertilizer thereof. The preservation number of the strain T-5 is CGMCC NO.8547. The T-5 containing living bacteria count of the strain T-5 containing microbial organic fertilizer disclosed by the invention is not less than 1.0*10<8> cfu/g. The invention also provides a method for preparing the strain T-5 containing microbial organic fertilizer. The strain T-5 disclosed by the invention is a specific efficient strain for inhibiting the growth of tomato bacterial wilt; the microbial organic fertilizer disclosed by the invention is rich in organic matters, amino acids and small molecule polypeptides, does not have a series of problems caused by the use of chemical pesticides, and both can be applied in feeding blocks and big fields; and the microbial organic fertilizer has the function of an organic fertilizer, and biocontrol bacteria contained in the microbial organic fertilizer are implemented through various growth promoting and biocontrol mechanisms, thereby achieving the effects of promoting growth and preventing and treating tomato bacterial wilt.

The invention relates to an antagonistic bacterium for controlling the bacterial wilt of capsicum and a microorganism organic fertilizer thereof, belonging to an agricultural intensified production technology. An antagonistic bacterium (bacillus subtilis a45) which plays an obvious antagonism role in pathogenic bacteria of the bacterial wilt of the capsicum is obtained by separation, and the disease-resistant microorganism organic fertilizer is prepared from the antagonistic bacterium and organic compost; and the fertilizer contains more than 2.0*109 a45 per gram, the total nitrogen content is 33.1g kg<-1>, the total phosphorus content is 13.3g kg<-1> and the total potassium content is 21.3g kg<-1>. Tests prove that the antagonistic bacterium can be rapidly bred after the microorganism organic fertilizer is applied to soil and forms a preponderant population in soil; and the preventing and treating rate on the bacterial wilt of the capsicum is as high as 70 percent.

The invention relates to growth-promoting bacteria for promoting ginger growth and preventing and controlling continuous cropping ginger soil-borne wilt and a microorganism organic fertilizer produced from growth-promoting bacteria, belonging to the technology of intensive agriculture production. A growth-promoting bacterial strain NJPRHSDAQ-1 is obtained through separation, and is inoculated into a microbial decomposition mixture of cured pig manure compost and rapeseed meal for solid fermentation so as to prepare the microorganism organic fertilizer, the fertilizer comprises more than 1*10<8>cfu/g of growth-promoting bacteria and 4-5% (above 90% of organonitrogen) of total nitrogen, the total nitrogen phosphorus and potassium nutrient is 6-10%, and the organic content is more than or equal to 45%. A pot experiment result proves that the rate for preventing and controlling the ginger soil from having the wilt achieves above 75%, and if being applied to soil in which the ginger is planted, microorganism organic fertilizer can prevent and control ginger soil-borne wilt.

The invention belongs to the technical field of the edible fungi cultivation and particularly relates to a culture medium and a cultivation method for cultivating black termitomyces albuminosus with enzyme bacterium residues as a main raw material. The culture medium is prepared from, by weight, 35-40 parts of enzyme bacterium residues, 20-25 parts of dried Pennisetum giganteum z.x.lin, 20-25 parts of cotton seed hulls, 13-18 parts of wheat bran, 1 part of sugar and 1 part of gypsum. The culture medium solves the problems that according to an existing cultivation method, a lot of cotton seed hulls are used, cost is high, the process is complex, the period is long, and edible fungus crude protein and microelement content is low. The enzyme bacterium residues generated after kitchen waste is manufactured into enzyme bacteria serves as the raw material, the cotton seed hulls and saw dust are properly added as main materials, black termitomyces albuminosus fruiting bags are cultivated, and the enzyme bacterium residues are environmentally friendly and contain various nutritional components which can be used by black termitomyces albuminosus for fruiting. Fruiting products are high in nutrient value and crisp and tender in taste, development of black termitomyces albuminosus and great development of the edible fungus industry are benefited, and the health level of human beings is improved.

The invention relates to an endogenous bacillus subtilis positive mutant strain, a preparation method thereof, a prepared biocontrol agent and an application of the biocontrol agent in preventing and controlling pomegranate dry rot, including (1) screening of a Bacillus subtilis wild-type strain NS04 and ultraviolet mutation and breeding of a positive mutant biocontrol strain NS04307; (2) antibacterial stability of the positive mutant strain NS04307; (3) a preparation method of the positive mutant strain NS04307 biocontrol agent; and (4) application of the positive mutant strain NS04307 in preventing and controlling plant diseases. The biocontrol agent disclosed by the invention has the characteristics of wide bactericidal spectrum, good effect, difficulty in disease drug resistance generation, safety on human, livestock and crops, environmental friendliness and the like and has a better effect on the field control of the pomegranate dry rot especially. In addition, the biocontrol agent disclosed by the invention achieves outstanding inhibition activity on the growth of 15 different pathogenic bacteria, such as Sclerotiumrolfsii, Trichotheciumroseum, Exserohilumrostratum, Fusariumoxysporum, Alternariaalternate and the like.

The invention provides a biologic bacterial strain XY21 for preventing and treating bacterial wilt in greenhouse vegetables, and pertains to the technical field for preventing and treating diseases in crops. The XY21 is verified to be Serratia sp., the culture preservation no. is CGMCC NO.2059. The XY21 bacterial strain expresses good colonization surrounding the roots of tomatoes. After soaking the culture for 14 days, the colonized volume of the bacterial strain surrounding a root of tomato tree is 5.05 is multiplied by 10<7>cfu/g soil. If the wet bacterial strain is mixed with a lab thallus-preservation solution in a proportion of 1:40 (g/ml), the bacterial strain can be stored 2-3 years under room temperature. Tests show that, by application XY21 bacterial strain in fields, the prevention effect is 64.84% and 79.80% respectively, the outputs of the diseased fields increased respectively 31.8% and 45.8%. The invention is of high prospective to be developed into commercial live-vaccine biologic preventing preparations.

The invention belongs to the technical field of crop disease prevention and treatment and discloses a bio-control bacteria strain 1JN2 for preventing and treating bacterial fruit blotches of watermelons and application thereof. The bio-control bacteria strain 1JN2 is named as Bacillus axarquiensis in classification, and is filed at General Microbiological Center of China General Microbiological Culture Collection Committee with CGMCC No.5731 on January 16, 2012. The bio-control bacteria strain 1JN2 is cultured in LB (luria-bertani) broth at the temperature of 28 DEG C by vibration of 180rpm for 12-16 hours, centrifuged by 6000rpm for 10 minutes, diluted by sterilization water to prepare bactericide, and total concentration of viable bacteria in the finished bactericide is 1X10<9>-1X10<10>CFU/ml. The experiment results in a greenhouse indicates that the bio-control bactericide with the 1JN2 has evident growth acceleration effect to watermelons, biomass live weight is increased to 55.92% and evident prevention effect to disease of fruit blotches of the watermelons, disease incidence can be reduced by 50.13%.

The invention relates to antagonistic bacteria S20 for controlling peanut bacterial wilt, an organic microbiological fertilizer prepared by the antagonistic bacteria S20, and applications of the antagonistic bacteria S20, belonging to the intensive agriculture production technology. The antagonistic bacteria which obvious antagonism effect on pathogenic bacteria of the peanut bacterial wilt, namely bacillus amyloliquefaciens S20, is obtained by separation, and an organic microbiological organic fertilizer is prepared by the antagonistic bacteria S20 and an organic compost, and includes more than 2.0*109 parts/g S20 cells, 30.5g kg-1 of nitrogen content, 12.1g kg-1 of phosphorus content, and 20.4g kg-1 of potassium content. As an experiment shown, the antagonistic bacteria can quickly breed after feeding the organic microbiological fertilizer into the soil, and dominant species can be formed in the soil, and more than 60% of treatment rate of peanut bacterial wilt can be achieved.

The invention discloses streptomyces having an inhibitory effect on phytophthorasojae root rot fungi, a biocontrol preparation and a preparation method of the biocontrol preparation, and belongs to the field of agricultural biotechnology. The strain (streptomyces) is streptomyces dorenlacusensis M1-4. The biocontrol preparation is obtained by inoculating a fermentation medium, which mainly takes soybean cake meal and wheat bran as main raw materials, with the streptomyces dorenlacusensis M1-4 which is subject to enlarged cultivation and by conducting fermentation culture, wherein the total number of spores of the biocontrol preparation is greater than or equal to 10<10> spores/g. From the key problem of preventing and controlling soybean diseases by virtue of bio-pesticide antagonistic microbe, the streptomyces dorenlacusensis M1-4 biocontrol preparation, which is capable of significantly relieving the occurrence of phytophthorasojae root rot, is researched and prepared, and the field preventing and controlling rate of the biocontrol preparation reaches 60% or above. With the application of the biocontrol preparation, the growth of soybean rhizosphere pathogenic microorganisms can be inhibited. Meanwhile, the biocontrol preparation can greatly reduce cost and can achieve a relatively good preventing effect.

The invention discloses antagonistic actinomycete for preventing and controlling continuously cropped strawberry root rot and a preparation method of a biocontrol agent of the antagonistic actinomycete. Bacterial strain B04 belongs to streptomyces hygroscopicus (Streptomyces hygroscopicus), with the strain preservation number of CGMCC NO.9918. The biocontrol strain B04 is separated from a healthy strawberry root, and has a strong inhibiting effect on pathogenic bacteria, such as fusarium, verticillium dahliae, rhizoctonia, phytophthora and the like, causing strawberry soil-borne root diseases. The antagonistic actinomycete prepared by taking wormcast and wheat bran as the main materials of fermentation medium can completely meet the nutritional requirements produced by solid fermentation of biocontrol antinomycetes. Compared with conventional fermentation, the production cost of the preparation method is reduced by 30 to 50 percent, and the preparation method is beneficial for popularization and application of the biocontrol agent of the antagonistic actinomycete. After the biocontrol agent of the antagonistic actinomycete is applied to continuously cropped soil, the biological control rate reaches greater than 70 percent, and has great significance on preventing and controlling the soil-borne diseases of strawberry, improving the quality of agricultural products and protecting the environment.

The invention relates to biocontrol bacterial strain B221 which can prevent the verticillum wilt of cottons, and the biocontrol bacterial strain B221 belongs to the technical field of the agricultural crop. The biocontrol bacterial strain B221 is identified as the chaetomium globosum. The preservation number of the strain is CGMCC NO.2493. The strain is shaken and cultured in PDA culture solution with 25 DEG C 200rpm for 7 days and is filtered through eight-layer of sterilization gauze to obtain the spore suspending liquid, the total concentration of the viable bacteria in the finished product is 10 <9> spores/ milliliters, therefore to obtain the biocontrol bacterial agent. The bacterial strain is preserved in the PDA inclined surface at 4 DEG C, the bacterial strain B221 represents better flat plate antagonism effect, greenhouse prevention effect and growth facilitation effect. The experiment shows that the greenhouse prevention effect of the bacterial strain B221 achieves 58.96 percent, the biomass increases by 51.20 percent; the biocontrol bacterial strain B221 has greater potentiality to be developed into the commercialized viable bacteria biocontrol agent.

A biological control bacteria formulation for preventing green house vegetable disease comprises Bacillus circulans, Bacillus pumilus, Bacillus cereus, Bacillus Subtilis, Serratia marcescens, Pseudomonas fluorescens. The preparation method is : mixing the above-mentioned bacteria according to 1:0.4-0.5:0.2-0.3:0.2-0.3:0.2-0.3:0.2-0.3, 200rpm shaking 36 hours in PDA culture liquid in 33 DEG C, 7000rpm centrifuging, preparing formulation using mixed bacteria and bacteria preservative fluid according to 1:40(g/ml), the alive bacteria concentration being 1x10<9>-1x10<14>cfu/ml. The bacteria formulation can be used in dunghill, root irrigation while transplanting after dilution. The preventing effect of bacteria formulation to vegetable disease can reach 75%, yield promoting effect is more than 45%.

The invention relates to a biocontrol strain JH21 for controlling rice sheath blight and rice blast, belonging to the technical field of plant protection. The bacteria are Pantoea agglomerans. The preparation method of the bacterial agent thereof comprises the following steps: using the bacteria to perform shaking cultivation in potato dextrose agar(PDA) culture solution at 30 DEG C in 180rpm for20-24 hours, then centrifuging in 6000rpm for 10 minutes, and using wet bacteria and the preservative fluid of the laboratory to prepare the bacterial agent according to a ratio of 1:40 (g/ml), wherein the total concentration of the live bacteria in the finished product is 3*10<9>-3*10<12>CFU/ml. The control rate of the JH21 bacterial agent to rice blast is about 65-75%, the yield can be increased by 40-50%; and the control rate to rice sheath blight is more than 60-70% and the yield can be increased by 40-60%. The JH21 bacterial agent can control rice sheath blight and rice blast simultaneously in one field; and the bacterial agent can obviously promote the growth of rice and increase the yield of rice. The bacterial agent can be used through soaking during seeding and after rice is transplanted, the bacterial agent is sprayed to control diseases.

The invention discloses a biocontrol strain HTC for preventing pepper phytophthora blight and application thereof, and provides a Chaetomium aureum strain HTC. The preservation number is CGMCC (China General Microbiological Culture Collection Center) No. 6173. The invention further provides a method for preparing an HTC biocontrol agent for preventing the pepper phytophthora blight. The method comprises steps as follows: the Chaetomium aureum strain HTC is fermented, so that a fermentation product is obtained, namely, the biocontrol agent is obtained. The experiment of the invention proves that in a greenhouse, the strain HTC biocontrol agent has a preventive effect on the pepper phytophthora blight to a certain extent, the effect can reach 72.19%, the strain height is increased by 12.27% and the live weight is increased by 32.28% in a seedling stage; and the live weight is increased by 31.11% and the yield of a pepper fruit is increased by 21.81% in reaping time.

The invention relates to anti-TMV (Tobacco Mosaic Virus) tripyrrole ring compound and a preparation method and application of the compound. The compound provided by the invention is a biological agent and particularly is a biocontrol agent especially developed for TMV, and the compound is completely free of a series of problems caused by application of chemical pesticides, which is beneficial to the pollution-free production of agricultural products.The compound provided by the invention can passivate TMV so that TMV loses infection activity. The compound also can induce the host to produce systemic resistance to alleviate and weaken the expression and occurrence of symptoms of TMV infected plants and the resistance effect on TMV is higher than 70%.