Antagonistic bacteria NJL-14 for preventing and controlling continuous-cropping tobacco bacterial wilt
A technology of NJL-14 and tobacco bacterial wilt, which is applied in the field of intensive agricultural production, can solve the problems of weakening the ability of sustainable agricultural development, loss of nutrient resources, waste of raw materials, etc., and achieves significant suppression effect, saving expenses and increasing income Effect
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[0026] (1) Isolation and identification of strains
[0027] The plants and surrounding soil that had experienced continuous cropping obstacles and severe bacterial wilt symptoms were collected, and the samples were stored at low temperature. The pathogenic bacteria - Ralstonia solanacearum (Ralstonia solanacearum) was isolated using Kelman's TTC Agar selective medium. Then, using the bacterial wilt pathogen as an indicator bacterium, the antagonistic bacteria were isolated from the rhizosphere soil of healthy tobacco plants from the continuous cropping tobacco bacterial wilt disease field. Then through the pot test and the field test, the high-efficiency antagonistic strains NJL-25 and NJL-14 were finally screened out, and the genera and species were identified. The strains were stored at -70°C in 30% glycerol. The diameters of the inhibition zone of antagonistic bacteria NJL-25 and NJL-14 were 24mm, 29mm ( figure 1 ).
[0028] The strain NJL-25 belongs to Brevibacillus brev...
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