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Antagonistic bacteria NJL-14 for preventing and controlling continuous-cropping tobacco bacterial wilt

A technology of NJL-14 and tobacco bacterial wilt, which is applied in the field of intensive agricultural production, can solve the problems of weakening the ability of sustainable agricultural development, loss of nutrient resources, and polluting the environment, and achieves significant suppression effects, cost savings, and biological control high rate effect

Active Publication Date: 2012-06-27
四川奥甘霖农业科技有限公司
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Problems solved by technology

[0004] On the other hand, the incineration of rice, wheat, corn, rapeseed and other crop straws, and the disposal of livestock and poultry manure after large-scale breeding not only seriously pollute the environment, but also greatly waste the organic fertilizer and bio-organic fertilizer products. Raw materials; a large number of nutrient resources (C, N, P, K, S and trace elements) are lost outside the soil-plant system, which obviously weakens the ability of my country's agricultural sustainable development

Method used

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  • Antagonistic bacteria NJL-14 for preventing and controlling continuous-cropping tobacco bacterial wilt
  • Antagonistic bacteria NJL-14 for preventing and controlling continuous-cropping tobacco bacterial wilt
  • Antagonistic bacteria NJL-14 for preventing and controlling continuous-cropping tobacco bacterial wilt

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Embodiment Construction

[0026] (1) Isolation and identification of strains

[0027] The plants and surrounding soil that had experienced continuous cropping obstacles and severe bacterial wilt symptoms were collected, and the samples were stored at low temperature. The pathogenic bacteria - Ralstonia solanacearum (Ralstonia solanacearum) was isolated using Kelman's TTC Agar selective medium. Then, using the bacterial wilt pathogen as an indicator bacterium, the antagonistic bacteria were isolated from the rhizosphere soil of healthy tobacco plants from the continuous cropping tobacco bacterial wilt disease field. Then through the pot test and the field test, the high-efficiency antagonistic strains NJL-25 and NJL-14 were finally screened out, and the genera and species were identified. The strains were stored at -70°C in 30% glycerol. The diameters of the inhibition zone of antagonistic bacteria NJL-25 and NJL-14 were 24mm, 29mm ( figure 1 ).

[0028] The strain NJL-25 belongs to Brevibacillus brev...

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Abstract

The invention relates to antagonistic bacteria NJL-14 for preventing and controlling continuous-cropping tobacco bacterial wilt, which belongs to intensive agricultural production technology. According to the invention, a microbial organic fertilizer is produced from two antagonistic bacteria (brevibacillus brevis NJL-25 and bacillus cereus NJL-14) which have a remarkable antagonistic function onpathogenic bacteria of the tobacco bacterial wilt and are obtained by separation, and organic fertilizers, wherein 1 gram of the microbial organic fertilizer contains more than 1*10<10> NJL-25 and 1*10<10> NJL-14, and has the total nitrogen content of 4 to 5 percent (organic nitrogen content is over 90 percent), the total nitrogen-phosphorus-potassium content of 6 to 10 percent and the content oforganic matters of 30 to 35 percent. Tests prove that the microbial organic fertilizer, after being applied to soil, can fast multiply the antagonistic bacteria to form a dominant group in the soil, achieve the biological prevention and control rate of over 97.6 percent on the bacterial wilt in the soil with the tobacco bacterial wilt, and prevent the occurrence of the continuous-cropping tobaccobacterial wilt to a great extent after being applied for long to the soil without continuous cropping obstacles.

Description

[0001] This application is a divisional application, the original application is, the name of the invention: antagonistic bacteria for the control of continuous cropping tobacco bacterial wilt and its microbial organic fertilizer, application number: 200910183359.6. 1. Technical field [0002] The invention relates to an antagonistic bacteria capable of preventing and eliminating bacterial wilt of continuous cropping tobacco and a microbial organic fertilizer made by using the antagonistic bacteria, which belongs to the technical field of agricultural intensive production and is specially used for overcoming and eliminating the problem of bacterial wilt of continuous cropping tobacco. 2. Background technology [0003] Tobacco is the main economic crop in my country, and its quality is related to the national economy and people's livelihood. Tobacco bacterial wilt is one of the main diseases of tobacco, which is seriously harmful and is called "smoke cancer". It was first dis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08C12R1/085
Inventor 沈其荣刘艳霞杨兴明徐阳春沈标
Owner 四川奥甘霖农业科技有限公司
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