Joint Control of Tomato Bacterial Wilt in Continuous Cropping with Chemical Agents and Biocontrol Microorganisms

A technology for tomato bacterial wilt and microbial strains, which is applied in the directions of microorganisms, microorganisms, and microorganisms to achieve significant biological control, significant control effects, and improved control effects.

Inactive Publication Date: 2021-04-16
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
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Problems solved by technology

At present, there is no effective chemical pesticide to control tomato bacterial wilt, and there is an urgent need to develop new technologies for the control of tomato bacterial wilt

Method used

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  • Joint Control of Tomato Bacterial Wilt in Continuous Cropping with Chemical Agents and Biocontrol Microorganisms
  • Joint Control of Tomato Bacterial Wilt in Continuous Cropping with Chemical Agents and Biocontrol Microorganisms
  • Joint Control of Tomato Bacterial Wilt in Continuous Cropping with Chemical Agents and Biocontrol Microorganisms

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Experimental program
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Embodiment Construction

[0027] (1) Isolation and identification of strains

[0028] Bacteria were isolated from the rhizosphere soil of tomato plants by the plate dilution method, and then the antagonistic actinomycetes were screened by the confrontation method with Ralstonia solanacearum (Ralstonia solanacearum), the pathogen of tomato bacterial wilt, as the indicator bacteria. Finally, the high-efficiency antagonistic strain B8 was screened out, and the strain B8 was identified as Bacillus amyloliquefaciens through morphological characteristics, physiological and biochemical characteristics and 16S rRNA gene sequence.

[0029] The strain B8 was deposited on April 17, 2017 in the General Microbiology Center of China Committee for Culture Collection of Microorganisms, and the strain preservation number is CGMCC NO.14042. The main biological characteristics are: when F8 grows on LB medium, the colony is gray and round, and the colony is smooth at the beginning, and there are wrinkles on the surface of...

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Abstract

The invention discloses the joint control and control of continuous cropping tomato bacterial wilt by chemical preparations and biocontrol microorganisms. The invention isolates an antagonistic bacteria (Bacillus B8) which has a significant antagonistic effect on the pathogenic bacteria of tomato bacterial wilt. The bacterial strain B8 belongs to amylolytic Bacillus amyloliqueBaciens was deposited on April 17, 2017 in the General Microorganism Center of China Committee for the Collection of Microbial Cultures, and the strain preservation number is CGMCC NO.14042. Use the antagonistic bacteria to produce liquid bacterial agents and microbial organic fertilizers, and the number of antagonistic bacteria in the liquid bacterial agents is greater than 10 9 / ml, the number of antagonistic bacteria in microbial fertilizers is greater than 5.0×10 7 pcs / g. Combined treatment with antagonistic bacteria liquid bacterial agent, microbial organic fertilizer containing antagonistic bacteria and 0.3% (W / V) potassium phosphite solution in continuous cropping tomato soil, the control rate of tomato bacterial wilt is as high as 70%.

Description

technical field [0001] The invention relates to the joint prevention and control of tomato bacterial wilt by using chemical agents, antagonistic antibacterial agents and microbial organic fertilizers, and belongs to the field of comprehensive prevention and control of plant soil-borne diseases. Background technique [0002] Tomato bacterial wilt is a soil-borne disease caused by Ralstonia solanacearum. The incidence rate is usually 20%-30%. It is especially serious in continuous cropping. Sometimes the incidence rate is as high as 70-80%, or even crop failure . At present, there is no effective chemical pesticide to control tomato bacterial wilt, and it is urgent to develop new technologies for the control of tomato bacterial wilt. We found that potassium phosphite (KH 2 PO 3 ), can be used as phosphorus and potassium fertilizer, and has the ability to inhibit the growth of pathogenic bacteria - R. solanacearum at a certain concentration. Potassium phosphite and antagonis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08C05F17/20C05F17/50C05G3/60C12R1/07
CPCC05B17/00C05F11/08C05G3/00C12N1/205C12R2001/07C05C11/00C05F3/00C05F17/00Y02W30/40
Inventor 沈标苏律杨兴明邱鹏飞沈其荣
Owner NANJING AGRICULTURAL UNIVERSITY
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