Joint Control of Tomato Bacterial Wilt in Continuous Cropping with Chemical Agents and Biocontrol Microorganisms
A technology for tomato bacterial wilt and microbial strains, which is applied in the directions of microorganisms, microorganisms, and microorganisms to achieve significant biological control, significant control effects, and improved control effects.
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[0027] (1) Isolation and identification of strains
[0028] Bacteria were isolated from the rhizosphere soil of tomato plants by the plate dilution method, and then the antagonistic actinomycetes were screened by the confrontation method with Ralstonia solanacearum (Ralstonia solanacearum), the pathogen of tomato bacterial wilt, as the indicator bacteria. Finally, the high-efficiency antagonistic strain B8 was screened out, and the strain B8 was identified as Bacillus amyloliquefaciens through morphological characteristics, physiological and biochemical characteristics and 16S rRNA gene sequence.
[0029] The strain B8 was deposited on April 17, 2017 in the General Microbiology Center of China Committee for Culture Collection of Microorganisms, and the strain preservation number is CGMCC NO.14042. The main biological characteristics are: when F8 grows on LB medium, the colony is gray and round, and the colony is smooth at the beginning, and there are wrinkles on the surface of...
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