303 results about "Gibellina cerealis" patented technology

The invention relates to a trichoderma harzianum composite bacteria culture and application of the same in the aspect of plant protection, and belongs to the technical field of biology. The strain of the trichoderma harzianum composite bacteria culture is preserved in the China General Microbiological Culture Collection Center (CGMCC), and classified and named as Trichoderma harzianum MDCGTH 18, with a preservation number of CGMCC NO.2965. The Trichoderma harzianum MDCGTH 18 composite bacteria culture utilizes antagonistic microbes for biological prevention and treatment, simultaneously has the functions of inducing the disease resistance of plants and increasing the yield, has obvious effect of controlling aspen root rot, apple root rot, citrus yellow shoot, watermelon fusarium wilt, tobacco bacterial wilt, wheat scab, full rot, root rot, banded sclerotial blight, cucumber powdery mildew, downy mildew, vegetable gray mold, fusarium wilt and various other epidemic diseases, can be independently taken as a biological control microbial inoculum for plant diseases, and can also be mixed with other biological antiseptics, plant antibiotics, plant growth regulators and the prior antiseptics to prepare various biological antiseptics for controlling different plant diseases.

The invention relates to bacillus amyloliquefaciens which has stronger phosphate solubilizing and plant growth promoting functions and has a better preventing and treating effect on various plant diseases. According to the bacillus amyloliquefaciens JCD-H-12 with the phosphate solubilizing, disease preventing and growth promoting functions, disclosed by the invention, the preservation number is CGMCC NO.11856, the preservation date is December 10, 2015, and multiple effects of solubilizing phosphate, preventing diseases, promoting the growth, increasing the yield and the quality, having a broad bacteriostatic spectrum and the like are obtained; a bacterial strain and inoculant of the bacterial strain are capable of effectively preventing and treating powdery mildew, tobacco black shank, botrytis blight, rice blast, cucumber fusarium wilt, tomato early blight, sweet pepper anthracnose, watermelon sclerotinia sclerotiorum, wheat scab, watermelon fusarium wilt, phytophthora capsici and the like; a broad-spectrum antibacterial effect is obtained, the effect is stable and durable, and the bacillus amyloliquefaciens can be developed into a bacterial fertilizer and microbial pesticide preparation which integrates phosphate solubilizing with disease preventing and treating.

The invention relates to a Bacillus amyloliquefaciense BA-KA3 and application thereof. The Bacillus amyloliquefaciense BA-KA3 is preserved in China General Microbiological Culture Collection Center on October 23, 2013, and has preservation No. CGMCC8342; its on tomato gray mold germs has obviously of antagonistic anti-role, on wheat scab bacteria. The Bacillus amyloliquefaciense BA-KA3 has obvious antagonistic effect on Fusarium graminearum, Rhizoctonia cerealis, Alternaria alternata, Phytophthora capsici, Colletotrichum capsici, Cercospora personata, Alternaria alternate and Garlic sclerotinia disease, and can significantly improve plant growth indexes such as germination rate, and budding rate and root length of seeds,and especially applies to preparation of agricultural preparations for control of tomato diseases due to Botrytiscinerea invasion and has certain growth promotion effect.

The invention relates to a wheat grain scab identification method based on imaging hyperspectral data. Compared with the prior art, the defect that a method for rapidly identifying wheat grain scab does not exist is overcome. The method comprises the following steps: acquiring a hyperspectral basic image; Preprocessing the hyperspectral basic image; Constructing a wheat grain scab recognition model; Training a wheat grain scab identification model; Obtaining a to-be-identified hyperspectral image; Preprocessing the to-be-identified hyperspectral image; And identifying wheat grain scab. According to the invention, indoor hyperspectral imaging data is utilized; According to the method, wheat grains are segmented in a hyperspectral image through an image segmentation technology, then spectralinformation of the wheat grains is subjected to comparative analysis, an effective identification model of wheat scab disease infected wheat grains is established through data noise removal and characteristic wavelength screening, and a rapid and effective method for wheat grain scab detection is formed.

The invention provides pseudomonas chlororaphis for preventing and treating the crop fusarium disease and applications thereof. The preservation number of the pseudomonas chlororaphis is CGMCC No. 7729. The Pcho01 bacterium strain of the pseudomonas chlororaphis has a high effective antagonistic action, is capable of being applied to the preventing and treating wheat scab caused by fusarium, and the preventing and treating efficiency in the field is maintained above 60%. The Pcho01 bacterium strain also has a very good antagonistic action on botrytis cinerea, rice sheath blight fungus, bipolaris maydis, pythium wltmum, rhizopus, and pseudomonas solanacearum, and can be used to prevent and treat the diseases caused by those bacteria mentioned above.

The invention provides Bacillus siamensis and application thereof, and belongs to the technical field of microbial prevention and control. Research shows that Bacillus siamensis LZ88 provided by the invention has very good effects on the biological control of plant diseases such as tobacco brown spot, tobacco black shank, wheat scab, grape anthracnose, cucumber fusarium wilt, peanut fusarium and the like, the induction of the resistance to tobacco plant diseases and the like, and provides a new strain resource for biological prevention and control of main diseases of tobacco, such as brown spot, and the like, thereby having good practical application value.

The molecular label closely linked with the active QTL of wheat fusarium blight resistance is prepared through hybridizing the disease-resistant wheat with the disease-sensitive wheat to obtain recombinant selfing line, and performing QTL and genetic linkage analysis to obtain the said molecular labels Xgwin 161, BARC 147 and Xgwin 493. They can be used to predict the level of the resistance to fusarium blight by detecting whether the DNA of wheat contains the said molecular labels or not.

The invention discloses a bacillus firmus strain. The bacillus firmus strain is bacillus firmus Bf-02 CGMCC No.3129, and can prevent and control a plurality of plant diseases, such as the anthracnose of walnuts, the gray mold of cucumber, wheat sharp eyespots, the ring rot of apples, tobacco brown spots, corn southern leaf blight, Dongzao jujube black patch, cotton fusarium wilt, cotton red rot, wheat scab and the like. Tests prove that the zymotic fluid of the bacillus firmus Bf-02 CGMCC No.3129 has remarkable effect of preventing and controlling vegetables, particularly the gray mold of the cucumber with the healthy growth of the vegetables and without hazards, and also remarkable growth promotion and yield increasing effects on the cucumber with the growth rate of 29.95 percent after one-time application.

The invention discloses a paenibacillus polymyxa of which the preservation number is CCTCC NO.M2015059 and an application of the paenibacillus polymyxa. The bacterial strain is capable of effectively inhibiting germination of fusarium graminearum spores and growth of hypha, inhibiting generation of fusarium toxin, and degrading generated zearalanel toxin; no toxic by-product is generated in the process of solving toxin pollution; secondary pollution does not exist; and the paenibacillus polymyxa can be used for preventing wheat scab, reducing the content of wheat DON toxin and zearalenone, and degrading the zearalenone in grains or feeds.

The invention provides actinomycetes which is streptomyces samsunensis XJC-2-6, and the preservation number is CCTCC NO:M 2017620. The streptomyces samsunensis XJC-2-6 provided by the invention has anantagonism on fusarium oxysporum f.sp cubense No. 4, curvularia fallax, curvularia lunata, btoryosphaeria dothidea, colletotrichum acutatum, colletotrichum gloeosporioides, colletotrichum fragariae,botrytis cinerea, fusarium oxysporum (schl.) f.sp cucumerinum owen, colletotrichum acutatum, pyricularia oryae cav, fusahum graminearum sehw, botryosphaeria dothidea and the like, has wide developmentspace on prevention and treatment of Panama disease of banana, microscler streak disease of banana, large grey speck disease of banana, tree canker of banana, anthracnose of pepper, anthracnose caused by colletotrichum gloeosporioides, anthracnose of strawberry, disease caused by botrytis cinerea, fusarium wilt of cucumber, anthracnose of mango, rice blast, wheat scab, ring rot of apple and the like, and has very good development and application prospects.

The invention discloses 2-sulfydryl-5-substituent group-1,3,4-thiadiazole (oxadiazole) derivatives with the function of preventing and controlling bacterial and fungal diseases of crops. Due to excellent physiological activity, the derivatives get more attention in the medical field, but the agricultural activity of the derivatives is still not reported at present. The research shows that the derivatives can be used for effectively inhibiting pathogens of such bacterial diseases as rice bacterial blight, rice bacterial leaf streak, tobacco bacterial wilt and tomato bacterial wilt and pathogens of such fungal diseases as wheat scab, potato late blight, apple canker, capsicum wilt, cucumber gay mold and sclerotia sclerotium. Meanwhile, when applied to prevention and control of the bacterial and fungal diseases of crops, the 2-sulfydryl-5-substituent group-1, 3, 4-thiadiazole (oxadiazole) derivatives has the advantages of simple structure, simple the preparation process, low the production cost and wide application prospect. The 2-sulfydryl-5-substituent group-1, 3, 4-thiadiazole (oxadiazole) derivatives has a general formula shown in the specification.

The present invention provides a complex pesticide of osthole and agricultural antibiotic. The effective components include osthole and agricultural antibiotic with the weight proportion of 1 to 6:1 to 8. The agricultural antibiotic is validamycin, kasugamycin or agricultural streptomycin. The complex pesticide of osthole and agricultural antibiotic provided by the present invention has the advantages of obvious additive effect, strong antibacterial activity and relatively small dosage and has obvious effect on prevention and treatment of rice sheath blight, wheat scab or rice blast.

The invention discloses an S-(5-substituted-1, 3, 4-thiadiazole)-(5-substituted phenyl)-2-furancarbothioic acid ester compound, a preparation method and application thereof. The compound has a structural general formula shown as formula I. The S-(5-substituted-1, 3, 4-thiadiazole)-(5-substituted phenyl)-2-furancarbothioic acid ester compound has an obvious control effect on potato late blight, cucumber fusarium wilt, Botrytis cinerea Pers, Pepper Phytophthora blight, rice sheath blight, wheat scab, Colletotrichum musae, asparagus stem blight and the like, and can be used as an agricultural fungicide in control of plant diseases. At the same time, the compound has an inhibitory effect on HL-60 human leukemia cells, MCF-7 human breast cancer cells, BGC-823 human gastric cancer cells, Bel-7402 human hepatocellular carcinoma cells, and KB human nasopharyngeal carcinoma cells, etc., and has application prospects in the aspect of anti-tumor activity. (formula I).

The invention discloses a near-infrared spectrum technology based method for rapidly identifying wheat grains with FHB (Fusarium Head Blight). The method is characterized by comprising the following steps of: collecting wheat samples with the FHB and acquiring multi-grain and single-grain spectral data of the wheat samples by using a near-infrared spectrometer; carrying out clustering analysis after spectral data preprocessing, and preliminarily observing the classification situation of the wheat samples; and confirming the grains with the FHB and identifying the sensitive waveband on the basis of the spectral data preprocessing, building identifying models of the multi-grain and single-grain wheat grains with the FHB through taking the sensitive waveband as variable input and finally realizing the rapid identification of the grains with the FHB. According to the invention, with a view to the fundamental research and discussion of the technology of separating the wheat grains with the FHB in China, a near-infrared spectrum technology is firstly used in the separation of the wheat grains with the FHB and a method for separating the wheat grains with the FHB, which is simple, convenient, easy and high in separation rate, is found; and the method disclosed by invention is capable of reducing the wheat grain impurity content, improving the flour quality and guaranteeing the human and animal safety and is a wheat separating and identifying technology which has board application prospect.

The invention discloses a method for detecting wheat scab based on deep mixing. The method comprises the following steps: S1, collecting the hyperspectral image pixel of wheat and wheat ear, and undersampling the hyperspectral image pixel of wheat and wheat ear to solve the problem of unbalanced data set, so as to obtain target data; S2, remodeling the two-dimensional image of the target data to obtain a gray-scale image, and preprocessing the gray-scale image based on the gray-scale image; S3, training the data by using a deep convolutional loop hybrid neural network based on the preprocesseddata; S4, analyzing the classification effect of the deep convolutional loop hybrid neural network model based on the training results. The invention utilizes the hyperspectral imaging technology tocarry out early rapid and non-destructive detection of wheat scab, and improves the accuracy of the regional classification result of the tested wheat.

The invention relates to a method for conducting high-throughput-testing on wheat gibberellic-disease-resisting genetic types and a kit thereof, in particular to SNP molecular markers used for conducting high-throughput-testing on wheat gibberellic-disease-resisting genes, an identifying method thereof, a method used for detecting the SNP molecular markers, a primer set and the kit.

The invention discloses a KASP primer group related to wheat scab resistance and application of the KASP primer group, and belongs to the field of crop breeding. The primer group comprises specific primers of which the nucleotide sequences are shown as SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3 in sequence; the KASP primer group can perform genotype identification on a molecular marker Qfhb.3AL17928, and experiments show that the wheat scab resistance of a wheat sample with the SNP locus genotype g is statistically superior to that of a wheat sample with the genotype a; the KSAP primer group canbe used for molecular marker-assisted selective breeding, and the gibberellic disease resistance breeding efficiency is improved.

The invention discloses a co-culture method of trichoderma atroviride and bacillus subtilis and an application of a co-metabolite obtained by the method. According to the method, the trichoderma atroviride SG3403 and bacillus subtilis 22 strains are used as co-culture objects, the inhibition rate of a co-culture fermentation liquid against fusarium graminearum is used as a response value, and thefermentation conditions such as culture temperature, a rotation speed, a pH, a corn flour content, a molasses content, a yeast powder content and a KCl content are optimized by a response surface method to obtain the following best fermentation conditions: the culture temperature is 28 DEG C, the rotation speed is 214 rpm, the pH value is 6.8, the yeast powder content is 10 g / L, and the molasses content is 27.6 g / L. The co-culture fermentation liquid cultivated by the formula provided by the invention improves the resistance to the fusarium graminearum, a substance not found in a single culture is found in the co-culture fermentation liquid, a seed coating agent prepared from the substance promotes the growth of wheat, and a novel option is provided for the development of biological pesticides and biological seed coating agents.

The invention discloses a pantoea agglomerans strain and application thereof. The pantoea agglomerans strain is named as Pantoea agglomerans ZJU23, with a preservation number of CGMCC No.16174 and a preservation date of July 30, 2018. According to the pantoea agglomerans strain, the control effect on wheat scab is up to 50-70%, vomitoxin is reduced by 50-80%, diseases caused by plant pathogenic fungi such as gibberellic disease, gray mold, rice blast, colletotrichum destructivum and rice sheath blight disease can also be simultaneously controlled, and the range of controlling is wide.

The invention relates to a biocontrol pseudomonas sp. and an application thereof, specifically to a biocontrol strain GZ9 with a preservation number of CCTCC M 20191037. The strain has a significant inhibition effect on botrytis cinerea, and shows a good inhibition effect on phytophthora capsici, magnaporthe grisea and gibberella zeae. The fermentation liquor of the strain has a good control effect on tomato gray mold, and can reach a control effect of 78.66%. Thus, the strain has good biocontrol application prospect.

The invention discloses a Paenibacillus polymyxa GY40 bacterial strain. The preservation number is CGMCC NO.14481. The invention also discloses a spore production fermentation culture method of the Paenibacillus polymyxa GY40 bacterial strain, and a method used for preparing bacterial powder and a wettable powder from the Paenibacillus polymyxa GY40 bacterial strain. Compared with terrestrial microorganisms, the Paenibacillus polymyxa GY40 bacterial strain possesses following advantages: salt resistance is excellent, growth temperature is low, and antibacterial effect is excellent. The spore production fermentation culture method is simple in formula; the main raw materials are agricultural product leftovers; the cost is low; the raw materials are widely available; bacterial strain growthspeed is high; fermentation temperature is low; fermentation time is short; fermentation conditions are convenient to control; fermentation spore yield is high; raw material cost is reduced; fermentation energy consumption is reduced; fermentation production efficiency is increased; the wettable powder possesses excellent preventing and treating effect on wheat scab and fusarium wilt of cucumber,and can be used in plantation of wheat and cucumber.

The invention relates to a bacteriocidal and insecticidal composite, and in particularly relates to a bacteriocidal and insecticidal composite which is capable of effectively preventing and controlling insect pests and diseases simultaneously. The bacteriocidal and insecticidal composite provided by the invention comprises a first active constituent and a second active constituent, wherein the first active constituent is 3-(2-chlorine-1,3-thiazole-5-methyl)-5-methyl-1,3,5-dioxazine-4-fork (nitryl) amine; the second active constituent is 2-cyano-3-amino-3-amino ethyl acrylate; and the weight percentage ratio of the first active constituent to the second active constituent is (1:100)-(100:1). The composite disclosed by the invention simultaneously has the effects of insect prevention and disease prevention, is improved in the aspect of medicine effect compared with the two chemicals used alone and very good in the effect of preventing and controlling the rice bakanae disease, thrip, wheat scab and aphid particularly.

The invention discloses a Talaromyces purpureogenus strain, a biocontrol agent and application of the Talaromyces purpureogenus strain. The classification name of the Talaromyces purpureogenus strain is Talaromyces purpureogenus TP18, and the accession number of the Talaromyces purpureogenus strain is CGMCC No. 22403. The strain has a good antagonistic effect on fusarium graminearum, and can be applied to prevention and treatment of wheat scab; and the biocontrol agent prepared from the fermentation broth of the strain has a strong inhibition effect on spore germination, hypha growth and vomitoxin yield of Fusarium graminearum, and has good application prospects.

The invention discloses bacillus velezensis and application thereof in degrading deoxynivalenol (DON). The bacillus velezensis has a collection number of CGMCC NO:18935 and is collected in CGMCC; andthe Latin name of the strain is bacillus velezensis, and a reference biomaterial (strain) is TP. A biopesticide preparation prepared by fermentation with the bacillus velezensis is used for controlling corn ear rot and wheat scab caused by fusarium verticillioides infection. The bacillus velezensis can be sprayed on corn ears at a corn flare stage, a spinning stage and a milk ripe stage or sprayedon wheat ears at a wheat flowering stage, and has high operability and good properties and stability.

The invention belongs to a molecule detection method of Fusarium graminearum to medium resistance level bacterial strain of carbendazim. The method can be used for monitoring drug resistance of Fusarium graminearum to carbendazim and prevalence warning, wherein Fusarium graminearum can cause wheat scab. The detection method includes the following three main steps: (1) nuclear genome DNA of bacterial strain to be detected is respectively extracted: (2) internal and external primer pairs are utilized to carry out nested PCR, thus obtaining a target segment; (3) the target segment is respectively restricted by restriction enzymes HindIII and TaaI (Tsp4CI), and genotype of medium resistance level bacterial strain can be accurately identified by virtue of a restriction product electrophoresis spectrum. By adopting PIRA-PCR (primer-introduced restriction analysis PCR) technology, quantity of Fusarium graminearum with medium drug resistance in field and ratio thereof in group can be rapidly and accurately detected. Detection accuracy reaches more than 95%.

The invention discloses a wheat scab monitoring method based on satellite remote sensing, and belongs to the technical field of remote sensing monitoring, and the method comprises the steps: firstly obtaining the remote sensing data and meteorological rainfall data of a wheat planting region in a predicted year and N years before the predicted year, wherein the remote sensing data comprise multispectral data and thermal infrared remote sensing data; calculating a relative growth vigor index by utilizing the multispectral data; obtaining a normalized surface temperature index by using the multispectral data and the thermal infrared remote sensing data; obtaining a normalized precipitation index by using the meteorological precipitation data; and finally, calculating the wheat scab incidenceindex of the predicted year by utilizing the relative growth vigor index, the normalized earth surface temperature index and the normalized rainfall index. According to the method, the interference of the difference of wheat growth vigor in different areas in large-scale monitoring on the gibberellic disease monitoring model can be well reduced, and the objectivity and practicability of gibberellic disease remote sensing monitoring are improved.

The invention provides a KASP primer group for detecting gibberellic disease resistance of Yangmai series wheat varieties (lines) and an application of the KASP primer group. The KASP primer group comprises a primer 1 with a nucleotide sequence shown as SEQIDNO.1, a primer 2 with a nucleotide sequence shown as SEQIDNO.2 and a primer 3 with a nucleotide sequence shown as SEQIDNO.3. The PCR amplification is carried out by taking the KASP primer group as a primer and taking a wheat genome to be detected as a template, then a PCR amplification product is detected by utilizing a multifunctional microplate reader and genotyping is carried out, and if a genotyping result shows that the genotyping of the wheat to be detected is the same as that of Yangmai No.4, the wheat to be detected contains an allele G; otherwise, the wheat to be detected contains the allele A; the gibberellic disease resistance of the wheat containing the allele G is higher than that of the wheat containing the allele A; the detection method can rapidly and visually obtain the result of the wheat variety gibberellic disease resistance genotype, and is suitable for large-scale breeding screening.

The invention discloses application of any compound of A-ring modified cryptolepine derivatives L-1 to L-10 in prevention and treatment of agricultural plant diseases. Activity test results show thatthe compound has potential inhibitory activity on four plant diseases such as sclerotinia rot of colza, rhizoctonia solani, botrytis cinerea and wheat scab, especially has an excellent antibacterial effect on botrytis cinerea, and is expected to be developed as a novel specific bactericide.

The invention particularly relates to a diagnosis method of the illness state severity of wheat scab in a field environment. The method comprises the following steps that (A) 45-degree-angle images ofwheat ears infected with the wheat scab in a wheat field are collected; (B) a field wheat ear dividing model is used for dividing the disease images to obtain wheat ear areas; a wheat scab dividing model is used for dividing the disease images to obtain disease scab areas; (C) a concave point matching method is used for dividing adhesion areas of the wheat ears and disease scabs to obtain the area of each wheat ear and the disease scab area of the corresponding wheat ear in the wheat field; (D) the ratio R of the disease scab area of each wheat ear to the area of the corresponding wheat ear is calculated, it is judged whether or not R is greater than a set threshold, and if yes, it is determined that the wheat ear is a disease ear; (E) the ratio X of the quantity of the disease ears to the total quantity of the ears in the wheat field is calculated, and the disease states are graded according to the ratio X. The invention also discloses a corresponding detection device. The method hasthe advantages that the diagnosis of the disease state of the wheat scab in the field environment can be achieved, the diagnosis speed is quick, and the accuracy is high.