Bacillus velezensis and application thereof in degrading deoxynivalenol (DON)

A technology of bacillus and microbial strains, applied in the direction of application, bacteria, fungicides, etc., can solve the problems of wheat ear grain shrinkage, carcinogenesis, and quality decline, and achieve good properties and stability, high control effect, and operability strong effect

Inactive Publication Date: 2020-07-10
ECOLOGY INST SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Infected ears of wheat not only shrunk their grains and decreased their quality, but also produced a variety of trichothecenes including deoxynivalenol, which can damage the immune system of humans and animals, and cause cancer. , teratogenic effect, a serious threat to the health and safety of humans and animals
However, there is no related research that Bacillus Velez can control corn ear rot and wheat head blight caused by Fusarium graminearum and can efficiently degrade deoxynivalenol toxin produced by Fusarium graminearum to report

Method used

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  • Bacillus velezensis and application thereof in degrading deoxynivalenol (DON)
  • Bacillus velezensis and application thereof in degrading deoxynivalenol (DON)
  • Bacillus velezensis and application thereof in degrading deoxynivalenol (DON)

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: Isolation and identification of bacteria

[0025] 1.1 Isolation of bacteria

[0026] (1) In the ultra-clean workbench, place the newly collected diseased ears of wheat and corn in 100 mL of sterile water, shake and rinse for 30 minutes, and suspend them sufficiently to make a bacterial suspension.

[0027] (2) After gradiently diluting the bacterial suspension with sterile water, take 100uL from each concentration of bacterial suspension dilution and spread it on the LB medium plate, place it at 30°C for 48 hours, and then pick it out on the plate Streak and purify single colonies with different shapes, sizes, and colors on LB plates, and number them.

[0028] (3) Take the purified strains of different numbers and culture them in LB liquid medium at 30°C and 180rpm for 24 hours, then take 2mL of fermentation liquid and mix them into 15mL of PDA medium, pour them on the plate, and inoculate cereals in the center of the plate after solidification Fusarium ...

Embodiment 2

[0036] Embodiment 2: the antagonism (co-cultivation method) of Velez bacillus TP to Fusarium graminearum

[0037] (1) After the purified TP strain was cultured in LB liquid medium at 30°C and 180rpm for 48 hours, 1mL of fermentation broth was evenly mixed into 15mL of PDA medium, poured onto a plate, solidified, and set aside.

[0038] (2) Use a hole puncher with a diameter of 0.5 cm to punch holes at the edge of the cultured pathogenic fungus Fusarium graminearum F18 colony to obtain bacterial flakes, and set aside.

[0039] (3) Use a sterile pick to pick the above-mentioned F18 bacterial slices into the center of the PDA plate in (1), and set the PDA plate inoculated with Fusarium graminearum F18 bacterial slices as the control group at the same time, and cultivate them at 30°C for 4-6 Day, observe the growth colony speed and colony radius of F18. Three replicates were set up for each, and the results are shown in Table 1.

[0040] Table 1 The inhibitory effect of TP on Fu...

Embodiment 3

[0044] Embodiment 3: TP fermented liquid detects the prevention and cure effect of potted corn ear rot

[0045] (1) Inoculate TP into LB culture medium, cultivate at 30°C and 180rpm for 72h, and obtain fermentation broth, with a biomass of 1.20×10 10 cfu / mL; adjust the bacterial concentration to 10 with tap water 9 CFU / mL, spare;

[0046] (2) The corn pot experiment was completed in the East Campus of Shandong Academy of Sciences from summer to autumn of 2018. The experiment used plastic flowerpots with a diameter of 33cm and a depth of 28cm. The soil was collected from the field cultivation layer, sieved and mixed with an appropriate amount of organic fertilizer. Each pot was filled with 14kg of soil. After watering, the Zhengdan 958 corn seeds were manually sown on June 20 , covered with 1.5cm thick fine soil. 21 days after emergence, 2 seedlings were set in each pot, watered quantitatively during the whole growth period, and managed normally. Set 4 treatments: CK contro...

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Abstract

The invention discloses bacillus velezensis and application thereof in degrading deoxynivalenol (DON). The bacillus velezensis has a collection number of CGMCC NO:18935 and is collected in CGMCC; andthe Latin name of the strain is bacillus velezensis, and a reference biomaterial (strain) is TP. A biopesticide preparation prepared by fermentation with the bacillus velezensis is used for controlling corn ear rot and wheat scab caused by fusarium verticillioides infection. The bacillus velezensis can be sprayed on corn ears at a corn flare stage, a spinning stage and a milk ripe stage or sprayedon wheat ears at a wheat flowering stage, and has high operability and good properties and stability.

Description

technical field [0001] The invention relates to the technical field of biopesticides, in particular to a bacillus velesii and its application in degrading deoxynivalenol. Background technique [0002] Bacillus velezensis is a Gram-positive bacterium that exists in various ecological environments such as plant rhizosphere, soil, plant interior, and rivers. This bacterium can promote plant growth, inhibit pathogenic bacteria, and improve its disease resistance It plays an important role in insect pests and in inducing plant disease resistance. It can inhibit a variety of plant pathogenic bacteria, prevent and control a variety of diseases, such as cotton verticillium wilt, cotton wilt, tomato gray spot, tobacco black shank, rice sheath blight, etc., and produce antibacterial proteins, lipopeptides and other antibiotics To resist pathogenic bacteria, secrete IAA and other substances to promote the development of plant root system and the intake of nutrients, promote plant grow...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L5/20A01N63/22A01P3/00C12R1/07
CPCA23L5/28A01N63/00C12R2001/07C12N1/205
Inventor 周红姿张新建周方园赵晓燕谢雪迎吴晓青张广志范素素王加宁
Owner ECOLOGY INST SHANDONG ACAD OF SCI
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