32 results about "Analysis pcr" patented technology

The invention discloses a method for identifying the varieties of low-phenol cotton. The genome DNA of each tested material is extracted; three SSR (simple sequence repeats) primers are respectively selected on 26 linkage groups of the cotton, so that the genome DNA of each tested material is used as a template for PCR (polymerase chain reaction) amplification; the PCR amplification result is analyzed; a fingerprint atlas of each variety of the low-phenol cotton is built; different varieties of feature primers are screened out; 53 pairs of primers express stable polymorphism among different tested materials; at least two pairs of feature primers are used and combined, and the varieties of the low-phenol cotton can be distinguished; the powerful technical support is provided for the identification, the development and the protection of the low-phenol cotton in future.

The present invention discloses a primer set for detecting bovine nornovirus and bovine coronavirus, the primer set comprises a primer for detecting the bovine nornovirus and a primer for detecting the bovine coronavirus, and the nucleotide sequences of the primer set are as shown in SEQ ID NO. 1-SEQ ID NO. 4. The invention also discloses a double RT-PCR method for detecting the bovine norovirus and the bovine coronavirus, and the steps are as follows: (1) synthesizing the primer set for detecting the bovine norovirus and the bovine coronavirus; (2) extracting RNA in a to-be-tested sample, andperforming inverse transcription on the extracted RNA as a template to obtain cDNA; (3) performing PCR amplification reaction on the cDNA obtained in the step (2) as a template by use of the primer set synthesized in the step (1); and (4) analyzing a PCR amplification product. The double RT-PCR method is simple, rapid, economical and efficient, and can greatly save detection time and improve detection efficiency.

The invention relates to a genetic constitution detecting method for a cloned specific T lymphocyte TCR BV CDR3 gene, which comprises the following steps: a single nucleus cell of peripheral blood or a general RNA in tissue sample is extracted and reverse-transcribed to cDNA. The upper reach primer of the TCR BV twenty four families and a common lower reach primer BC are designed according to TCRBV gene; the cDNA is expanded using real-time fluorescent quantification PCR with dye method. The PCR product is analyzed with the melting curve; the negative primary derivative (-dF/dT) of fluorescence intensity change of the PCR product is graphed vs. the temperature (Tm); the peak graph of BV each family is obtained; the BV family PCR product showing single peak is purified and processed with serial analysis; the sequencing results are compared with the TCR Beta chain standard gene sequence. The distribution of the cloned specific T lymphocyte in tissue or peripheral blood is judged according to the appearing situation of single peak in BV each family peak graph; the genetic constitution of the cloned specific TCR BV CDR3 is determined combining the sequencing results. The genetic constitution detecting method for the cloned specific T lymphocyte TCR BV CDR3 gene has important significance in evaluating diseases related to specific cloned T cell.