The invention discloses a kit for detecting bovine circovirus, which comprises a primer for detecting bovine circovirus, an RNA extracting reagent, a reverse transcription reagent, a positive controland a negative control, wherein the nucleotide sequence of the primer is an upstream primer P1: 5 '-GGTGCCGTTGTTGTGTCA-3 ', downstream prim P2: 5'-CAGCCCTGAGTTGCCTTATC-3'; Positive control is BCoV, BNoV, BAstV, BRV, BVDV and BKV, negative control is sterile double distilled water. The invention also discloses an RT-A PCR method comprises the following steps: (1) synthesizing the primers for detecting bovine circovirus; (2) extracting RNA from the sample to be tested, and reverse transcribing the extracted RNA as a template to obtain cDNA; (3) PCR amplification reaction is carried out by usingthe cDNA prepared in the step (2) as a template and the primer set synthesized in the step (1); (4) PCR amplification products are analyzed. Simple, practical, economical and efficient, it can greatlyimprove the detection efficiency and save the detection time.