Polymerase chain reaction detection method of hepatitis B virus genotyping

A genotyping and genotyping technology, applied in the direction of microbial determination/inspection, biochemical equipment and methods, material stimulation analysis, etc., can solve problems such as product contamination, non-typing, high experimental conditions and requirements, and achieve high compliance The effect of high efficiency and easy operation
CN102140537BActive Publication Date: 2013-04-03THE FIRST AFFILIATED HOSPITAL OF FUJIAN MEDICAL UNIV
0 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
THE FIRST AFFILIATED HOSPITAL OF FUJIAN MEDICAL UNIV
Publication Date
2013-04-03

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention provides a polymerase chain reaction (PCR) detection method of hepatitis B virus (HBV) genotyping. The method comprises the following steps: a primer is designed according to the entire genome sequence of the HBV genotype; in the existence of the primer, DNAs are extracted from a serum sample to perform PCR amplification in a fluorescence PCR meter, the PCR product is analyzed according to the melting curve, the genotype is judged according to the Tm value of the PCR product and the HBV genotyping can be realized. The PCR melting curve method used in the invention is convenient to operate, the detection result of the method has higher coincidence rate with the detection result obtained by adopting a commercial genotyping kit; and the method can be used in the clinical detection of the HBV genotype. On the basis of the PCR technology, the PCR melting curve method which is to judge the HBV genotype according to the Tm value, is provided in the PCR detection method, thus the HBV genotype can be identified conveniently, rapidly and accurately.
Need to check novelty before this filing date? Find Prior Art

Description

Technical field

[0001] The invention relates to a PCR melting curve detection method for HBV genotyping. Background technique

[0002] Hepatitis B virus (HBV) mainly causes liver damage through the host immune mechanism. The body's immune system recognizes viral antigens and attacks the infected liver cells to cause inflammation. This process is affected by multiple factors including the host and the virus. Viral gene heterogeneity affects the expression of antigens and also plays an important role in this process. Different strains have different frequencies of certain mutations, different strains' resistance to immune clearance, and other factors may cause different genotypes to have different post-infection disease spectrum.

[0003] HBV is usually divided into eight genotypes from A to H, based on the heterogeneity of the whole gene sequence ≥ 8%, or the heterogeneity of the S gene sequence ≥ 4%. HBV genotypes are distributed in a certain geographical area. Type C is predomi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More