36 results about "Hbv genotype" patented technology

The invention provides a method and kit for detecting genotype of hepatitis B virus, the method includes following steps: 1, extracting DNA genome of HBV from blood serum or blood plasma; 2, designing typing premer and probe according to conserved sequence of HBV genome; 3, using micromolecule marked oligonucleotide primer to proceed PCR reaction to amplify target DNA sequence; 4, oligonucleotide probe is added with a tail chain poly dC, then UV cross-linking on substrate and baking at a temperature of 120 DEG for fixing; 5, hybridizing amplication product of the marked target DNA with specific oligonucleotide probe on substrate; 6, detecting bybridization conjugates to judge different genotypes of HBV. The invention designs specific probe with high conservative and PCR amplication primer in different base position, and improves detection sensitivity, such that HBV genotypes can be detected quickly, conveniently and accurately.

The invention relates to the field of biotechnology. At present, determined genotypes of hepatitis B virus (HBV) comprise eight types A, B, C, D, E, F, G and H, and the pathogenicity, the medicine resistance and the treatment effects of antiviral medicaments vary with the genotypes. In order to identify the HBV genotypes of clinical patients and patients to be tested, the invention provides the gene-sequencing-method-based hepatitis B virus genotyping kit, and a method for identifying the genotypes comprises the following steps of: (1) extracting an HBV deoxyribonucleic acid (DNA) genome fromserum or blood plasma; (2) designing genotyping primers according an HBV S area; (3) performing amplification on a target DNA fragment by a polymerase chain reaction (PCR); (4) detecting a gene sequence of the target DNA by a gene sequencing instrument; and (5) judging the genotype of HBV according to the gene sequence of the target DNA of the HBV. The kit is accurate in result and high in sensitivity, can be operated with high flux, is convenient to popularize and use, and provides a basis for the clinical treatment and rational administration of medicaments for HBV patients.

The present invention provides methods, kits, and oligonucleotides for detecting and analyzing the nucleotide sequence of a reverse transcriptase (RT) region of the polymerase (Pol) gene of Hepatitis B Virus (HBV). In certain embodiments, a target RT region is amplified and subjected to DNA sequencing. The sequence obtained is compared to one or more DNA sequences characteristic of an HBV genotype or serotype, and / or one or more DNA sequences characteristic of an HBV mutation that confers resistance to a drug or vaccine, to determine the HBV genotype or serotype of the amplified product and / or the presence or absence of one or more DNA sequences characteristic of an HBV mutation that confers resistance to a drug or vaccine.

The invention relates to an intelligent equipment based HBV genotyping lateral flow multiplex reagent detection device. The device includes intelligent equipment, a communication unit, a core control unit, a current regulation unit, an excitation light source and a high pass optical filter. The intelligent equipment communicates with the core control unit through a bluetooth communication way; the excitation light source is used for exciting the fluorescent substance on a lateral flow multiplex detection reagent, and the exciting light intensity is realized by setting the current regulation unit by the core control unit; and the intelligent equipment shoots the fluorescence image of the lateral flow multiplex detection reagent after passing through the high pass optical filter, image processing is carried out according to a built-in algorithm, the genotype of a to-be-detected sample on the lateral flow multiplex detection reagent is judged, and finally the detection result is displayed. The intelligent equipment is adopted as the system software and image analysis carrier, the structure of the HBV genotyping device is simplified, the detection device has the advantages of small volume, quick detection, simple operation, low cost, and easy promotion, and the detection result can be uploaded to various medical institutions through a wireless network of the intelligent equipment.

The invention discloses a PCR (polymerase chain reaction) primer system for the detection of HBV (hepatitis b virus) nucleoside analog drug-resistant mutation sites, a method and application. The PCRprimer system comprises a first amplification primer pair, wherein the sequences of an upstream primer and a downstream primer in the first amplification primer pair are respectively as shown in SEQ ID NO:1 and SEQ ID NO:2. The PCR primer system, method and related application can be used for detecting HBV genotype, particularly nucleoside analog drug-resistant mutation sites, thereby being beneficial to the judgment of HBV prognosis and treatment effect, offering help, such as auxiliary clinical medicine, to formulate a personalized antiviral treatment schedules for chronic hepatitis B patients, and ensuring that clinical medicine is more reasonable and more effective. The PCR primer system is high in amplification effectiveness which can reach greater than 97 percent, good in reproducibility, especially low in the minimum detectability, and beneficial to the detection of HBV nucleic acid concentration samples.

A method for predicting prognosis (clinical outcome) of hepatocellular carcinoma patients based on the detection of a Hepatitis B virus, determination of the HBV genotype, identification of the strain of the HBV genotype and its integration into the genome, in biological samples of such patients is provided.

The invention discloses a PCR (polymerase chain reaction) primer system for the detection of HBV (hepatitis b virus) nucleoside analog drug-resistant mutation sites, a method and application. The PCRprimer system comprises a first amplification primer pair, wherein the sequences of an upstream primer and a downstream primer in the first amplification primer pair are respectively as shown in SEQ ID NO:1 and SEQ ID NO:2. The PCR primer system, method and related application can be used for detecting HBV genotype, particularly nucleoside analog drug-resistant mutation sites, thereby being beneficial to the judgment of HBV prognosis and treatment effect, offering help, such as auxiliary clinical medicine, to formulate a personalized antiviral treatment schedules for chronic hepatitis B patients, and ensuring that clinical medicine is more reasonable and more effective. The PCR primer system is high in amplification effectiveness which can reach greater than 97 percent, good in reproducibility, especially low in the minimum detectability, and beneficial to the detection of HBV nucleic acid concentration samples.

The present invention is a diagnostic kit and materials for: 1) the prediction of the long-term response of a chronic hepatitis B virus (HBV) carrier to treatment with nucleoside / nucleotide analog, or their combination; 2) the detection of HBV variants that exhibit reduced reactivity to antibody detection; 3) the detection of HBV variants in the precore / core region that negatively affect the course of liver disease; 4) the identification of the HBV genotype.

The invention provides a method and a kit for detecting the genotype of hepatitis B virus, the method comprising the following steps: (1) extracting the HBV DNA genome from serum or plasma; (2) designing according to the conserved region of the hepatitis B virus genome Typing primers and probes; (3) Use small molecule labeled oligonucleotide primers to perform PCR reaction to amplify target DNA fragments; (4) Add poly dC tails to oligonucleotide probes, and spot them on the substrate for ultraviolet radiation After linking, bake and fix at 120°C; (5) Use the labeled target DNA amplification product to hybridize with the specific oligonucleotide probe on the substrate; (6) Detect the hybridized conjugates to determine the different genotypes of HBV. The invention designs highly conserved type-specific probes and PCR amplification primers at different base positions, improves detection sensitivity, and can perform HBV genotyping quickly, conveniently and accurately. The invention also provides a test kit for HBV genotyping, which is clinically used for detecting the genotype of hepatitis B virus, can provide reliable basis for clinical treatment and rational drug use, and is worthy of popularization and use.

A probe combination for detecting cancer includes one or more sets of partial hepatitis B virus (HBV) targeting probes. When sequences of each of the sets of partial HBV targeting probes are aligned, an overall sequence of the aligned set of probes matches a reference sequence of a genome of a HBV genotype or a direct repeat (DR) region on the genome. In the aligned set of probes, each of the probes overlap with one or two adjacent probes by a portion of a length of the probe. The probe combination may further includes one or more sets of hotspot gene targeting probes targeting cancer hotspot genes such as CTNNB1, TERT, and TP53 genes, one or more sets of exogenous gene targeting probes targeting portions of a lambda phage genome, and endogenous gene targeting probes targeting endogenous genes such as GAPDH and GdX genes.

The invention provides a polymerase chain reaction (PCR) detection method of hepatitis B virus (HBV) genotyping. The method comprises the following steps: a primer is designed according to the entire genome sequence of the HBV genotype; in the existence of the primer, DNAs are extracted from a serum sample to perform PCR amplification in a fluorescence PCR meter, the PCR product is analyzed according to the melting curve, the genotype is judged according to the Tm value of the PCR product and the HBV genotyping can be realized. The PCR melting curve method used in the invention is convenient to operate, the detection result of the method has higher coincidence rate with the detection result obtained by adopting a commercial genotyping kit; and the method can be used in the clinical detection of the HBV genotype. On the basis of the PCR technology, the PCR melting curve method which is to judge the HBV genotype according to the Tm value, is provided in the PCR detection method, thus the HBV genotype can be identified conveniently, rapidly and accurately.

The invention relates to the field of biotechnology. At present, determined genotypes of hepatitis B virus (HBV) comprise eight types A, B, C, D, E, F, G and H, and the pathogenicity, the medicine resistance and the treatment effects of antiviral medicaments vary with the genotypes. In order to identify the HBV genotypes of clinical patients and patients to be tested, the invention provides the gene-sequencing-method-based hepatitis B virus genotyping kit, and a method for identifying the genotypes comprises the following steps of: (1) extracting an HBV deoxyribonucleic acid (DNA) genome fromserum or blood plasma; (2) designing genotyping primers according an HBV S area; (3) performing amplification on a target DNA fragment by a polymerase chain reaction (PCR); (4) detecting a gene sequence of the target DNA by a gene sequencing instrument; and (5) judging the genotype of HBV according to the gene sequence of the target DNA of the HBV. The kit is accurate in result and high in sensitivity, can be operated with high flux, is convenient to popularize and use, and provides a basis for the clinical treatment and rational administration of medicaments for HBV patients.