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PCR primer system, method and application for detection of hbv nucleoside analog drug-resistant mutation site

A technology of drug-resistant mutation sites and nucleoside analogs, applied in the field of PCR primer systems, can solve the problems of differences in the efficacy of antiviral therapy in patients with chronic hepatitis B, and achieve high sensitivity, low detection limit, and good reproducibility. Effect

Active Publication Date: 2019-03-01
黑龙江金域医学检验实验室有限公司
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Problems solved by technology

Different genotypes of HBV have differences in the efficacy of antiviral therapy for patients with chronic hepatitis B

Method used

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  • PCR primer system, method and application for detection of hbv nucleoside analog drug-resistant mutation site
  • PCR primer system, method and application for detection of hbv nucleoside analog drug-resistant mutation site
  • PCR primer system, method and application for detection of hbv nucleoside analog drug-resistant mutation site

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Embodiment Construction

[0025] In order to facilitate the understanding of the present invention, the present invention will be described more fully below with reference to the associated drawings. Preferred embodiments of the invention are shown in the accompanying drawings. However, the present invention can be embodied in many different forms and is not limited to the embodiments described herein. On the contrary, these embodiments are provided to make the understanding of the disclosure of the present invention more thorough and comprehensive.

[0026] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field of the invention. The terms used herein in the description of the present invention are for the purpose of describing specific embodiments only, and are not intended to limit the present invention. As used herein, the term "and / or" includes any and all combinations of one or more of ...

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Abstract

The invention discloses a PCR (polymerase chain reaction) primer system for the detection of HBV (hepatitis b virus) nucleoside analog drug-resistant mutation sites, a method and application. The PCRprimer system comprises a first amplification primer pair, wherein the sequences of an upstream primer and a downstream primer in the first amplification primer pair are respectively as shown in SEQ ID NO:1 and SEQ ID NO:2. The PCR primer system, method and related application can be used for detecting HBV genotype, particularly nucleoside analog drug-resistant mutation sites, thereby being beneficial to the judgment of HBV prognosis and treatment effect, offering help, such as auxiliary clinical medicine, to formulate a personalized antiviral treatment schedules for chronic hepatitis B patients, and ensuring that clinical medicine is more reasonable and more effective. The PCR primer system is high in amplification effectiveness which can reach greater than 97 percent, good in reproducibility, especially low in the minimum detectability, and beneficial to the detection of HBV nucleic acid concentration samples.

Description

technical field [0001] The invention relates to the field of molecular biology detection, in particular to a PCR primer system, method and application for detecting HBV nucleoside analog drug-resistant mutation sites. Background technique [0002] HBV is a blood-transmitted hepadnavirus. According to the nucleotide sequence heterology of the whole HBV gene ≥ 8% or the nucleotide sequence heterology of the S gene region ≥ 4%, different virus strains can be divided into different types. genotype. So far, HBV can be divided into 8 genotypes, namely A, B, C, D, E, F, G and H. The genotypes of HBV in my country mainly include types B and C, in addition to a small number of types A, D and C / D recombinants. [0003] HBV is an important factor leading to chronic hepatitis, liver cirrhosis and liver cancer. Antiviral therapy is the most common treatment for hepatitis B. Recognized antiviral drugs for HBV treatment mainly include interferon and nucleoside (acid) drugs. Nucleoside...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/686
CPCC12Q1/686C12Q1/706C12Q2600/118C12Q2600/156
Inventor 郭晓磊冯宇鹏曾征宇徐艳艳张鹏博柳盛伟郝必喜
Owner 黑龙江金域医学检验实验室有限公司
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