35results about How to "Shorten the experimental process" patented technology

The invention is about the PCR detecting method of a respiratory tract pathogen and the reagent box. The character is to design the vector and the probe according to the highly special base groups of the pathogen by analyzing the nucleic acid sequence of the pathogen. So next to amplify the PCR or the RT-PCR in the fluorescent PCR instrument.

The invention relates to a fluorescent PCR detecting method for a HBV type seperating and the reagent box. The characteristics of the method lies in finding out the HBV DNA complete sequence which has been seperatedd from the GenBank to make a sequence; according to the result of the sequence matching, finding out the gathering zone of the type specific base of the BHA gene type, designing a probe and a pair of primers according the gathering zone. Extract HBV DNA from the blood serum sample to make PCR amplification to realize HBV gene type seperating detection in the fluorescent PCR when the probe and primer exist. The invention provides a reagent box and the type positive standard used in it. The accuracy of type parting reaches to 98% and can reach to 100% by adjusting the reaction condition., simpler and quicker, the test procedure and the time consuming is less, the cost is lower than the complete sequence analysis and more quick and accurate than the regular relevant PCR and RFLP method, besides, the closed check adopted in the invention can avoid the contamination efficiently.

The invention discloses a specific amplification primer for detecting marssonina coronaria. A method for quickly obtaining gene segments of the marssonina coronaria by using the specific primer aims to prevent obstruction brought to actual researches by the difficult separation of pathogenic bacteria. The invention also discloses a rapid molecular detection method for the marssonina coronaria by using the specific amplification primer, which comprises the following steps of: first collecting field diseased leaves and picking and placing the acervulus of the marssonina coronaria on a white paper sheet with a sterilized insect needle; then preparing a polymerase chain reaction (PCR) system; next transferring the acervulus into a small PCR amplification tube in which the PCR system is arranged and performing centrifugation to completely immerse the acervulus in the prepared PCR system; and finally performing PCR amplification and detecting the amplification product. By changing the PCR amplification reaction system and the amplification procedure, the target segment of the pathogenic bacteria can be directly amplified. The primer and the method prevent the separation of the pathogenic bacteria and additional DNA extraction, shorten a test flow and improve the detection efficiency.

The invention discloses a component optimization design method of austenite Fe-Mn-Al-C light alloy steel, and relates to the technical field of metal materials. The component optimization design method comprises the steps that S1, typical chemical components are formulated; S2, thermodynamic and phase diagram calculation is carried out; S3, a corresponding relation graph of the precipitation temperature and calculated SFE is drawn; S4, the chemical components of the alloy are optimized; and S5, chemical component and experiment detection is carried out. The typical chemical components of the alloy steel are formulated on the basis of the chemical component range limited by the austenite Fe-Mn-Al-C light alloy steel, an Olson-Cohen thermodynamic model is selected to calculate the stacking fault energy (SFE) of the typical alloy steel, all the chemical components are optimized, a deformation mechanism of materials can be predicted through the method, the generation content and precipitation temperature of kappa-C carbide are controlled, the strengthening effect of the kappa-C carbide on the austenite light steel is achieved, and a theoretical basis is provided for alloy design and production.

The invention relates to a fruit fly culture and dosage device for a laboratory, and belongs to the technical field of fruit fly experiment devices for the laboratory, which solves the technical problems of replacing of different culture bottles and the like in the fruit fly culture and dosage process of the prior art. The fruit fly culture and dosage device for the laboratory adopts the solutionthat an upper section and a middle section, as well as the middle section and a lower section are respectively connected by a threaded connecting opening; a bottle cork is arranged at the upper end surface of the upper section, a medicine liquor adding pipe penetrates through the bottle cork and is communicated with the upper section, and the middle part of the side wall of the upper section is provided with an anesthetic air inlet I; a culture medium is arranged at the bottom part of the lower section, and the middle part of the side wall of the lower section is provided with an anesthetic air inlet II; a screen plate is arranged at the threaded connecting port I, and a blocking plate is arranged at the threaded connecting port II; a handle is used for driving the blocking plate or the screen plate to rotate from the 9 o'clock position to the 12 o'clock position along the pointer circular rotary track by a rotary shaft. The fruit fly culture and dosage device has the advantages that the problem of requiring of multiple steps and different culture bottles in the fruit fly dosage process is solved, and the experiment device integrating multiple steps is provided.

The invention provides a device and a method for carrying out acidolysis by adopting an I-type glass tube. The device comprises the I-type glass tube for providing an acidolysis reaction space, a magnet, a magnetic stirrer, a sample boat for storing samples, a connecting joint arranged at a top tube opening of the I-type glass tube and a magnetic stirrer, wherein an acidolysis reaction solution iscontained in the I-shaped glass tube, the magnet is located on one side of the outer wall of the I-shaped glass tube, the magnetic stirrer is located on one side of the inner wall of the I-shaped glass tube, and the magnet and the magnetic stirrer are attracted and fixedly arranged on the I-shaped glass tube. The sample boat is placed in the I-shaped glass tube in a manner that an opening is upward, and the sample boat is supported on the magnetic stirrer; by adopting a symmetrical structure, on the basis of ensuring the quality of experimental data, the operation is simpler and more convenient, the experimental process is greatly simplified, and the risk of experimental failure is reduced.

The invention provides a vacuum filter, which is characterized in that a mass of filtering of fluid solution such as a tissue culture solution can be completed by an operator without a clean room, cleanliness degree is ensured, the experiment flow is shortened, and the work efficiency of the operator can be increased. The vacuum filter comprises an upper cup and a lower reception bottle, the upper part of the upper cup is provided with a lid, the vacuum filter is characterized in that a filtering apparatus is arranged between an lower outlet of the upper cup and an upper inlet of the lower reception bottle, the inlet of the filtering apparatus is connected to the lower outlet of the upper cup, the outlet of the filtering apparatus is communicated with the upper inlet of the lower reception bottle, a filter membrane is arranged in the filtering apparatus, a pipe joint is arranged at the side part of the filtering apparatus, one end of the pipe joint is communicated with an internal cavity of the filtering apparatus, and the other end of the pipe joint is externally connected to a vacuum pump.

The invention discloses rubber containing copper tailings as well as a preparation method and application of the rubber, and relates to the field of rubber materials. The rubber is prepared through the following steps that natural rubber, zinc oxide, an anti-aging agent and stearic acid are mixed, then copper tailings and Si69 are added in two times for mixing, and then rubber discharging is conducted; mixing a product obtained after internal mixing with an accelerant and sulfur, carrying out open milling process treatment, standing, and then carrying out a vulcanization process, so as to obtain the rubber. Interface bonding is generated between the copper tailings and the rubber through Si69, the bridge connection effect is achieved, the compatibility of the copper tailings and the rubber is improved, agglomeration is reduced, on the premise that the filling amount of the copper tailings is increased, all properties of the rubber are improved, the production cost of the raw materials is reduced, and the mechanical strength and wear resistance of the rubber are improved; and the temperature rise value of compression heat generation is relatively low, so that the performance requirements when the material is applied to specific products can be met.

The invention provides a urine exosome RNA extraction and library construction method for an NGS platform, and belongs to the technical field of medicine and biology. Effective sedimentation of exosome is realized by adding a sedimentation aid reagent into urine, the problem of limitation of RNA materials is solved, meanwhile, a Trizol reagent is adopted to directly perform RNA extraction on the exosome and remove redissolution and purification steps, loss and degradation of RNA in extraction are reduced, and qualified raw materials are provided for subsequent library construction. By repairing cDNA damage, optimizing terminal repairing and A adding, purifying steps and the number of library amplification reaction cycles, PCR amplification is performed by using a high-fidelity enzyme premixed solution to establish a library, the library preserves exosome molecular diversity, and a reliable sample is provided for application of an NGS technology in liquid biopsy and diagnosis. The method effectively solves the problems that the exosome content of a urine sample is low, the total amount of extracted RNA is low, a library cannot be effectively built, and sequencing is conducted through an NGS platform.

The invention relates to a high-throughput library construction kit for detecting thalassemia gene mutation. The kit comprises multiple pairs of primer probe sets corresponding to nucleotide sequencesin a sample to be detected, exonuclease, nucleic acid ligase, nucleic acid polymerase and other library building reagents. The invention also relates to a library construction method for detecting thalassemia gene mutation. The method comprises the following steps of: 1, providing a reaction system; step 2, performing hybridization; 3, conducting enzyme digestion and purification; 4, extending, connecting and purifying; and 5, enriching. The kit and the library construction method provided by the invention have the advantages of whole genome library construction, simplicity in operation, short experimental steps, low cost, high accuracy, enrichment efficiency up to 80% or above and the like.

The invention discloses a lithium-sulfur battery positive electrode material and a preparation method thereof. Specifically, the lithium-sulfur battery positive electrode material is a sulfur-carbon composite material containing porous-structured carbon with in-situ doped nitrogen and sulfur elements. The preparation method of the lithium-sulfur battery positive electrode material comprises (1) ina high-temperature inert gas atmosphere, carbonizing silkworm excrement to obtain a carbon material with in-situ doped nitrogen and sulfur elements; (2) activating the carbon material through potassium hydroxide to obtain porous carbon; (3) mixing the porous carbon with sulfur at high temperature to obtain the sulfur-carbon composite positive electrode material. According to the sulfur-carbon composite positive electrode material, the carbon and the active sulfur achieve relatively strong physical and chemical effects to effectively inhibit shuttle effects and meanwhile can improve the utilization rate and the structural stability of sulfur. The prepared lithium-sulfur battery positive electrode material is excellent in electrochemical performance and low in cost and has a broad application prospect.

The invention provides a construction method of a CRX (Clustered Regularly X) gene deletion mutation cat mutant, which comprises the step of knocking out partial sequences of one or more exon sequences of a wild CRX gene of a cat to ensure that the CRX gene cannot be normally expressed. According to the obtained CRX gene knockout cat, a disease animal model with huge application value is provided for medical research, and a foundation is laid for promoting research of hereditary eye diseases and screening of eye disease drugs.

The invention relates to a preparation method of high-purity chlorogenic acid. The method comprises the following steps: S1, taking a honeysuckle medicinal material, crushing, adding into 15-25 timesby weight of pure water, and soaking for 2-4 hours; S2, adding hydrochloric acid to adjust the pH value to 3-4, heating to 45-60 DEG C, extracting twice, and filtering to obtain a first-stage extracting solution; S3, directly loading the first-stage extracting solution obtained in the step S2 on a polyamide column, performing gradient elution by adopting an eluent containing 10-40 vol% of an extracting agent, and collecting the fractions to obtain a second-stage extracting solution; S4, concentrating the second-stage extracting solution obtained in the step S3 to 8-20% of the original volume of the first-stage extracting solution so as to obtain a third-stage extracting solution, adding ethyl acetate according to a volume ratio of the third-stage extracting solution to the ethyl acetate of1:(3.5-4.5), extracting twice, and combining filtrates to obtain a fourth-stage extracting solution; and S5, concentrating the fourth-stage extracting solution obtained in the step S4 under reduced pressure until the volume of the fourth-stage extracting solution is 75-88% of the original volume of the fourth-stage extracting solution, and refrigerating and crystallizing to obtain the chlorogenicacid. The method has the advantages that impurities are removed to a large extent, and the purity of chlorogenic acid is effectively improved.

The invention relates to the field of cell separation and purification, in particular to a method for separating inner ear FZD10 positive glial cells. According to the method for separating the inner ear FZD10 positive glial cells, the inner ear FZD10 positive glial cells are subjected to fluorescence labeling by virtue of transgenic mouse hybridization, so that purified inner ear glial cells can be conveniently obtained subsequently by virtue of flow sorting. According to a separation and purification technology adopted by the method, the glial cells can be obtained to the maximum extent, the activity of the cells can be kept, the purity of the separated FZD10 positive glial cells reaches 98% or above, the requirements of follow-up experiments can be met, analysis and purification process is simple and rapid, and the method is very suitable for follow-up experiment research.

The invention relates to the field of cultural relic detection, and discloses a method for preparing jute cellulose enzymatic hydrolysis product conjugation proteins for detecting cultural relics. The method includes degreasing jute celluloses by the aid of benzene and ethyl alcohol; removing lignin by the aid of an acetone-formic acid-water mixed system; removing hemicelluloses by the aid of alkali liquor and then carrying out enzymatic hydrolysis on obtained celluloses by the aid of cellulase and beta-glucosidase; recycling enzymes by the aid of ultra-filtration technologies after enzymatic hydrolysis is carried out; carrying out freeze drying on filtrate to obtain glucose powder; dissolving the glucose powder and egg white proteins in deionized water and potassium bromide solution to obtain solution; placing the solution in a constant-temperature and constant-humidity tank; carrying out freeze drying to obtain glucose-egg white protein conjugates. The method has the advantages that conjugation macromolecules obtained by the aid of the method are stable and can be used as complete antigens, immune injection can be carried out on animals to obtain corresponding specific antibodies, and accordingly jute fabrics in the cultural relics which are ancient textiles can be feasibly authenticated.

The invention discloses a low-initial-quantity DNA library construction method. The method comprises the following steps: taking genome DNA, adding a random primer marked with biotin, and carrying outannealing and an amplification reaction; separating and purifying a whole genome amplification product; carrying out tail end supplement on the separated and purified product to obtain linear blunt end DNA; adding ligase into the filled linear blunt end DNA to carry out connection reaction; carrying out purifying to obtain double-stranded annular DNA, and then breaking the double-stranded annularDNA into linear DNA fragments; capturing a linear DNA fragment labeled with biotin by using streptomycin magnetic beads; carrying out tail end repair on the captured linear DNA fragment and adding anA tail base for reaction; connecting joints to two ends of the linear DNA fragment subjected to the reaction by adding the A tail base; and carrying out PCR amplification on the product of the linkerto obtain a DNA library. According to the method, a small amount of genomic DNA is randomly amplified by using biotin-labeled random primers, and amplification, truncation and biotin labeling of thegenomic DNA are realized at the same time, so that construction of a large-fragment DNA library with low initial input is realized.

The invention relates to the technical field of biological medicines, and particularly discloses a novel T cell immune modulator bioactivity detection method. Eukaryotic expression plasmids and co-expression resistance genes of sleeping beauty transposase and reporter genes driven by different response elements are expressed, eukaryotic expression plasmids of transposon inverted repetitive sequences are inserted into two ends of the reporter genes and the co-expression resistance genes and jointly transferred into host Jurkat T cells, pressurized screening and monoclonal separation are performed to obtain stable monoclonal effect cells, target cells and T cell activating modulators are added, and bioactivity of T cell immune modulator is measured by measuring activity of the reporter genes. The method can be applied to a bioactivity reporter gene detection system for cell immune modulators such as PD-1 / PD-L1 monoclonal antibodies and CTAL4-Fc fusion proteins. Any human blood-derived cell components or other components are omitted, testing results are stable, operation is simple and convenient, testing time is short, and the method is quite suitable for later drug quality control and batch release detection and can be widely applied.

The invention relates to a durable flame-retardant low-smoke polyester fabric and a preparation method thereof, belonging to the technical field of textile finishing. The present invention adopts post-finishing method to process and arrange the surface of polyester fabric, and by means of high-temperature and high-pressure impregnation method, the polyester fiber is expanded, the surface pores are increased, the specific surface area is increased, the molecular movement is intensified, and the cyclic phosphate ester and inorganic nano-particle flame retardant At the same time, it enters the interior of the polyester fabric, and after returning to normal temperature, the flame retardant can be firmly wrapped inside the polyester fabric, making the flame-retardant polyester durable and flame-retardant and smoke-suppressive. In the preparation method of the invention, the flame retardant and smoke suppression can be completed in one step, the production steps are reduced, the flame retardant and smoke suppression effects are good, and at the same time, it has durability and is convenient for industrialized production.

The invention provides a method for preparing nitrogen-doped graphitized carbon. Metal ions are introduced into a nitrogen-doped carbon material body, after a mixture is subjected to high-temperaturethermal treatment, then the metal ions are removed, and the nitrogen-doped graphitized carbon material is obtained. The method for preparing nitrogen-doped graphitized carbon is simple, the particle size is small, graphitized carbon prepared by the method is used as a lithium ion battery cathode material, the specific capacity of 354.6 mAh / g can be achieved, the circulation performance is excellent, and after 100-time circulation, the capacity retention ratio is stabilized at 99%.

The invention provides a method for rapidly purifying and isolating anti-actin F(ab')2 and Fc fragments, which comprises a proteolysis step and a protein A magnetic bead purification step. The method provided by the invention is simple and convenient to operate, high in purification efficiency, accurate and reliable.