52results about How to "Increase the speed of enzymatic hydrolysis" patented technology

The invention provides a preparation process of resveratrol, which relates to the field of medicament preparation and solves the problems of low preparation conversion rate, low yield and poor product quality of resveratrol in the prior art. The preparation process of resveratrol comprises the following steps: washing away dust of giant knotweed used as raw material, cutting the giant knotweed into slices or preparing the giant knotweed into coarse powder for later use; adding material to be used to a decomposition pool and adding enzymolysis liquid to carry out enzymolysis; and extracting. Through repeated scientific research improvements, compared with the traditional process, the invention shortens the enzymolysis time by half, improves the enzymolysis quantity by one time and improves the conversion rate by one time. A special enzymolysis process is designed for the preparation process, the operation convenience, the use effectiveness and the conversion reliability greatly exceed that of the traditional process, thereby the quality of finished products is ensured and stabilized more effectively, the cost is reduced by half, and the social benefit is obvious.

The invention discloses a glycated serum protein determination kit. The kit contains a reagent R1 and a reagent R2; the reagent R1 contains Tris buffer solution, trypsin, peroxidase, an anti-interference substance, a protein denaturant, an ionic liquid, CaCl2, a surfactant and a preservative; and the reagent 2 contains the Tris buffer solution, fructosaminase, 4-aminoantipyrine, sodium 3-(N-ethyl-3-methylanilino)-2-hydroxypropanesulfonate (TOOS), a surfactant, a stabilizer and a preservative. The kit disclosed by the invention is a liquid kit high in accuracy and good in stability. The invention also discloses a preparation method and application of the kit.

The invention discloses a preparation method of wheat protolysate, belonging to the technical field of food processing. The preparation method comprises the following steps: by taking wheat protein as a raw material, mixing slurry, pretreating, neutralizing, adding complex enzyme for enzymatic hydrolysis reaction and deactivating enzyme and spray-drying to obtain a product wheat protolystate. Citric acid is adopted to pretreat wheat protein to have deamidation, the solubility of the wheat protein can be increased, the subsequent protease is favorably close to an enzyme digestion site, the enzyme utilization rate, solubility and enzymolysis speed can be increased. The wheat protolysate prepared by adopting the method is good in solubility, dispensing with obvious bitter taste, the content of small peptide is high, the protein is high digestion rate and easy to absorb, the bioavailability of the wheat protein can be greatly improved; and the preparation technology is simple, the time consumption is short, enzyme investment cost is low, and the preparation method is suitable for industrial production.

The invention provides xanthoceras sorbifolia protein peptide and a preparation method thereof as well as a protein peptide health-care product. The preparation method of the xanthoceras sorbifolia protein peptide comprises the following steps: step A: carrying out enzymolysis reaction on xanthoceras sorbifolia cake meal in water; carrying out enzyme inactivation and removing dreg and collecting enzymolysis liquid, wherein enzymolysis conditions are as follows: the pH (Potential of Hydrogen) is 7 to 10 and an enzyme is one or more of papain, bromelain, flavored proteinase, alkaline proteinase, neutral proteinase and trypsase; step B: carrying out ultra-filtration on the enzymolysis liquid and collecting filtrate with the molecular weight less than or equal to 1000Da. The protein peptide health-care product is mainly prepared from the following raw materials in parts by weight: 40 to 50 parts of the xanthoceras sorbifolia protein peptide in claim 7, 8 to 16 parts of a sweetener and 3 to 5 parts of a sour agent; the protein peptide health-care product has a form of granules, tablets, capsules or an oral solution. The method provided by the invention deeply develop and use the xanthoceras sorbifolia cake meal to extract highly-purified protein peptide, turning waste into wealth; according to the method provided by the invention, the xanthoceras sorbifolia cake meal is sour, sweet and delicious, is easy to preserve and has a wide consumer group.

The invention discloses a coconut oil extraction method, which comprises the following steps of grinding coconut flesh into pulp; performing microwave ultrasonic processing on the coconut pulp; performing enzymolysis and centrifugation; taking upper layer clean oil to obtain coconut oil. When the coconut oil extraction method provided by the invention is used, under the conditions of not using organic solvents or not adopting high-temperature processing, the damage to nutrition substances is reduced; the method is simple and safe; the oil yield is high; the cost is low; the energy consumption is low; residue liquid after the oil and grease extraction contains rich protein and polypeptide and can be recovered and reused; the residue solid contains rich dietary fiber and polysaccharide, and can be used as animal feed or can provide carbon sources for microorganism fermentation; waste water can be easily processed; substances harmful to environment cannot be generated; the prepared coconut oil is clear and transparent, has intense coconut fragrance and has good application prospects.

The invention discloses a method for extracting longan pulp polysaccharide. The method comprises the following steps of (1) slurrying and slurry treatment: mixing fresh longan pulp and purified water, pouring a mixture into a homogenizer for slurrying, adding alkali liquor and activating at -4 to 10 DEG C for 10-12 hours to obtain an activated liquid; (2) enzymolysis and concentrating treatment: adjusting the pH value of filtrate, adding a copper oxide-zirconium oxide catalyst and ternary complex enzyme to filtrate, carrying out enzymolysis under the condition of 200-500W of microwave irradiation and then carrying out ultra-filtration concentration on enzymatic hydrolysate obtained through enzymolysis; (3) centrifugal treatment: mixing a concentrated liquid and a mixed alcohol solution, and collecting an educt by adopting a centrifugal machine; and (4) spray drying treatment. An alkali liquor low-temperature activating enzyme association technology is introduced into an extraction preparation process of the longan polysaccharide; the yield of a target product is obviously improved in comparison with a conventional technology; and meanwhile, the method has the characteristics of being efficient, fast, low in cost and suitable for massive industrial production.

The invention discloses amino acid-enriched bovine bone powder and a preparation method thereof. The invention relates to the technical field of bovine-resource product deep-processing, and especially discloses amino acid-enriched bovine bone powder which is prepared by deep-processing the bovine bones and a preparation method thereof. A purpose of the amino acid-enriched bovine bone powder is to solve the problems of insufficient developments and utilizations of the fresh bovine bone resources; and the fresh bovine bone resources are deeply developed by modern science and technology, so that the bovine bone can be better absorbed and utilized by the human body. The amino acid-enriched bovine bone powder is successfully prepared from the fresh bovine bones by a normal-temperature high-pressure steam-cooking method and a double-enzyme fractional degradation method. The prepared amino acid-enriched bovine bone powder is high in protein content and strong in antioxidant activity; and the amino acid amount is no lower than 27.54%, which is 34.33-40.34% higher than the amino acid amounts of unprocessed bovine bones, so that the amino acid-enriched bovine bone powder is high in biological values. Thus, the amino acid-enriched bovine bone powder can be taken as a good source of high-quality human proteins.

The invention relates to a preparing method of honey medicinal liquor and a preparing method of honey health-caring medicinal liquor, and belongs to the field of liquor making. According to the honey medicinal liquor, honey serves as a raw material, low-content honey fermented liquor is obtained after dilution and fermentation, and liquid-state distilling is carried out on the honey fermented liquor to obtain medium-high-content honey distilled liquor; namely, base liquor is obtained. Three kinds of traditional Chinese medicinal materials including schisandra chinensis, boschniakia rossica and acanthopanax senticosus are added into the base liquor, and effective ingredients in the medicinal materials are extracted to the maximum degree with an ultrasonic compound-enzyme-assisting extracting method. According to the honey liquor, parts of nutritional ingredients and the health caring function of the natural honey are reserved, and physiological activators are increased; the honey liquor serves as the base liquor of the honey medicinal liquor, various traditional Chinese medicinal materials are matched, and the obtained honey medicinal liquor is high in ingredient nutritive value and honey infiltration degree; then the prepared low-content health-caring medicinal liquor both has the flavor of the honey liquor and strong traditional Chinese medicine smells, and has the effects of improving sleeping and resisting fatigue and oxidization.

The invention discloses a sialic acid electrochemical test paper as well as a preparation method and a detection method thereof. The sialic acid electrochemical test paper comprises an insulating substrate, a conductive electrode, an insulated isolation layer, a reaction layer, a pretreatment layer, a siphon sample guide groove and an adhesion spacer; the reaction layer is an enzyme reaction membrane; the enzyme reaction membrane covers the surfaces of a reference electrode and a working electrode of a reaction region and is arranged at the reaction region end of the insulated isolation layer;the pretreatment layer is arranged at the front end of the reaction layer; the reaction layer and the pretreatment layer are arranged in the siphon sample guide groove; the enzyme reaction membrane comprises an enzyme which is reacted with sialic acid and an electron transfer mediator; and slurry of the pretreatment layer contains a sialic acid hydrolytic agent. For the sialic acid electrochemical test paper provided by the invention, by using a method of detecting the sialic acid with a direct electrochemical biosensor principle, by arranging a sample pretreatment structure and combining enzyme in an enzyme layer, the sialic acid in blood is rapidly and thoroughly decomposed into free sialic acid, so that the test sensitivity of the sialic acid is improved, and a method of accurately, rapidly and simply detecting the sialic acid with low cost is realized.

The invention discloses a method for preparing sesame oil by enzymolysis and supercritical fluid extraction. The method comprises the following steps: (1) preparing sesame pulp, namely smashing sesame into fine powder of 100-300 meshes, adding water in 5-7 times by weight of the sesame, uniformly stirring, heating to 40-50 DEG C, and performing heat preservation for 15-20 minutes, so that the sesame pulp is obtained; (2) carrying out enzymolysis, namely regulating pH of the obtained sesame pulp to 4.0-5.0, adding compound enzyme accounting for 0.2-0.3% of the weight of the sesame pulp and carrying out enzymolysis; and (3) carrying out supercritical fluid extraction, namely placing the sesame pulp subjected to enzymolysis in step (2) into an extraction kettle, and carrying out supercritical extraction by taking supercritical CO2 fluid as an extraction solvent and an ethanol solution with volume concentration of 60-70% as an entrainer, so that the sesame oil is obtained. Compared with the prior art, the method for preparing the sesame oil by enzymolysis and supercritical fluid extraction has the advantages that oil yield is high, technology is simple, production efficiency is high and safety and reliability are realized.

The present invention is health food containing protein material and its derivative, and is health food compounded with oyster and scallop enzymolizing liquid and soybean lecithin. The health food contains oligopeptide, amino acid, polysaccharide, taurine, lecithin, vitamins and minerals. The key points of the present invention includes superfine treatment of oyster and scallop into 10 microns below slurry before enzymolysis, the addition of soybean lecithin and the microcapsulizing treatment in high speed shearing and mixing emulsifying machine.

The invention discloses a compound ethanol enzyme with acid proteases and a method for preparing the compound ethanol enzyme. The compound ethanol enzyme and the method have the advantages that the high-activity acid proteases and plant extract with abundant plant-based enzymes and nutrient substances are particularly compounded on the basis of scientific compound food-grade enzyme preparations, so that the compound ethanol enzyme is provided with complete enzyme systems; activators, protective agents and antioxidants are further added into the high-activity acid proteases and the plant extract, the enzyme activity can be completely released by the aid of the activators and can be stabilized by the aid of the protective agents and the antioxidants, and hops extract and novasil which are used for inhibiting bacteria contamination and promoting saccharification of leaven and quick proliferation and fermentation of saccharomyces cerevisiae are further scientifically compounded; starch, proteins, fat, cellulose, hemi-cellulose, pectin and the like in unprocessed grain can be sufficiently and effectively hydrolyzed, accordingly, the raw material utilization rate and the liquor yields can be effectively increased, and the alcohol contents can be increased by 13.63%, 36.25% and 17.44% respectively as compared with commercially available special compound ethanol enzymes; the liquor yields of raw materials can be respectively increased by 17.41%, 38.81% and 20.04%, and the ethanol production cost can be obviously reduced.

The invention provides a preparation method of chicken soup cream, the chicken soup cream, application and a use method. The chicken soup cream is prepared by the following steps of boiling whole Sanhuang chicken, crushing into a chicken pulp, and adding multiple types of enzyme to perform ultrasonic enzyme hydrolysis reaction; adding L-cysteine hydrochloride, glucose, yeast extract and hydrolyzedvegetable protein liquid to perform maillard reaction; finally, adding chicken oil, monosodium glutamate and edible salt to flavor, and packaging, so as to obtain the chicken group cream. The chickengroup cream can be prepared into chicken soup, hotpot base soup, rice noodles, pasta soup base, stuffing and the like; the chicken soup cream and water can be mixed according to a ratio of 1:20 to 1:40, so as to obtain the soup material; the chicken soup cream and the foods can be mixed according to a ratio of 1:100 to 3:100, so as to obtain the stuffing. The chicken soup cream has the advantagesthat the nutrients are rich, the taste is delicious, the preparation time is short, the energy consumption is low, and the enzyme hydrolysis rate is high; the convenience in use is realized, and theapplication range is broad.

The invention belongs to the technical field of glucan, and particularly relates to a green preparation method of yeast glucan. The green preparation method includes the steps: 1 adding common yeast cell walls into distilled water, uniformly stirring the common yeast cell walls and the distilled water to form suspension liquid, heating the suspension liquid at constant temperature for 2-4 hours, adding tourmaline and hydrogen peroxide, performing sealed cooking for 2-4 hours, keeping the temperature for 2-6 hours, taking out the tourmaline and performing centrifugation to obtain precipitates;2 adding the precipitates into the distilled water, uniformly stirring the precipitates and the distilled water, adding dispersing agents, heating and stirring the dispersing agents and the distilledwater to form turbid liquid, adding alkaline liquid, and adjusting pH (potential of hydrogen) to be 9-11 to obtain alkaline turbid liquid; 3 adding protease and the tourmaline into the alkaline turbidliquid, performing constant-temperature enzymolysis for 4-6 hours, taking out the tourmaline and then performing centrifugation and washing to obtain insoluble substances; 4 adding the insoluble substances into acetone, performing microwave reaction for 2-5 hours, and filtering and freeze-drying reactants to obtain yeast glucan powder. The yeast cell walls serve as production raw materials, the tourmaline serves as an auxiliary catalyst and an auxiliary oxidizer, and the yield of the yeast glucan is greatly improved.

The invention discloses an extraction and preparation method of small molecular fish skin collagen peptide. The extraction and preparation method comprises the following steps: preparing raw materials, carrying out enzymolysis pre-treatment, carrying out enzymolysis treatment and the like. According to the extraction and preparation method of the small molecular fish skin collagen peptide, provided by the invention, fish skin is firstly subjected to degreasing treatment and then is subjected to the enzymolysis treatment; and in an enzymolysis treatment process, various types of different proteinases are used for carrying out enzymolysis on the fish skin, so that the enzymolysis speed can be improved, the dosage of the proteinase also can be reduced, and the yield of the small molecular fish skin collagen peptide can be effectively improved. According to the extraction method provided by the invention, modified activated carbon is used for de-coloring, the adsorption performance is excellent and enzymolysis liquid can be uniformly distributed; good de-coloring and deodorization effects can be realized under the conditions of small dosage and short time and effective components are not lost; and the extraction method has the advantages of low investment, simplicity in operation, good protein degradation effect and high yield of the fish skin collagen peptide, the industrial large-scale production is easy to realize and the extraction method has good application value and market potential.

The invention provides instant berry powder and a making method thereof and belongs to the field of food processing. The instant berry powder is prepared from the following raw materials in parts by weight: 35-40 parts of blueberry, 25-30 parts of mulberry, 10-15 parts of raspberry, 10-12 parts of strawberry, 6-10 parts of gooseberry, 3-5 parts of ficus carica, 8-12 parts of grape, 3-5 parts of kiwi fruit, 4-6 parts of carambola, 5-8 parts of papaya, 3-5 parts of psidium guajave and 4-8 parts of passiflora edulis. The making method comprises the following steps: freezing the raw materials, pressing, then adding an active substance for enzymatic hydrolysis, filtering an enzymatic hydrolysate and mixing with an auxiliary material and maltodextrin, wherein the auxiliary material is prepared by radix puerariae, poria cocos, radix angelicae, semen nelumbinis and peppermint leaves; drying to obtain the instant berry powder. The instant berry powder provided by the invention is powdery and granular, convenient to eat, better in dissolubility and mouth feeling, specific in berry aroma and sweetness, rich in nutrition and difficult for moisture absorption in air and has the healthcare effects of resisting aging, reducing blood pressure and the like.

The invention discloses a fiber raw material pretreatment device which comprises a device body and a cam starting cavity arranged in the device body. A power driving device is arranged in the cam starting cavity and comprises a multifunctional motor fixedly arranged in the right-side inner wall of the cam starting cavity, and a cam cavity is arranged in the left-side inner wall of the cam startingcavity in a communicated manner. By the fiber raw material pretreatment device, pretreatment of fiber raw material wheat straw can be realized, crystallinity of cellulose can be lowered through a physical method of chopping and grinding, automatic enzymolysis can be performed after grinding, and tiny fiber raw material particles obtained by grinding improve digestion rate of fiber and accelerateenzymolysis speed; few power sources are adopted to complete multiple process steps, so that energy consumption is lowered, and resources are saved.

The invention relates to a production system of oil cake functional polypeptide. The production system comprises a pulverizer, a dissolving bottle, an extracting tank, a collecting bottle, ultrasound-assisted enzymatic preparation devices, a segregating unit, and an ultrafilter. The pulverizer is connected with the dissolving bottle. The extracting tank is respectively connected with the dissolving bottle and the collecting bottle through pipelines. A pump is arranged on the pipeline between the extracting tank and the dissolving bottle, and a suppressor is arranged on the pipeline between the extracting tank and the collecting bottle. The extracting tank is placed in a water tank, and an ultrasonic oscillator is disposed in the extracting tank. The collecting bottle is connected with the ultrasound-assisted enzymatic preparation devices which are sequentially connected with the segregating unit which is connected with the ultrafilter. According to the production system, oil cakes are further smashed by means of the ultrasonic oscillator, and the production efficiency of the functional polypeptide is improved.

The present invention discloses a preparation method of saikosaponin. Raw materials comprise 10 g of rhizome bupleuri, 120 g of 90% of ethanol, compound biological enzymes, low-carbon alcohol and macroporous adsorption resin. The preparation method is meticulous in steps, firstly, the rhizome bupleuri is crushed, a plant fiber structure is destroyed, during enzymatic hydrolysis, contact areas withan enzymatic hydrolysate can also be increased, an enzymatic hydrolysis speed of the plant fibers in the rhizome is accelerated, the biological enzymes comprise cellulase, protease and pectinase, canconduct the enzymatic hydrolysis of cell walls of the rhizome bupleuri and other biological substances, and fully destroy the original biological structure, so that saponin can be released, then multiple ethanol extractions can extract the saponin from the rhizome bupleuri, then extraction with ethanol is used, an obtained primary material is subjected to twice extraction, and impurities thereinare further separated. The preparation method can obtain the high-purity saponin and content of the fine impurities in the obtained saponin is less than that of the existing saponin.

The invention discloses a high-efficiency crude heparin sodium production technology, and relates to the technical field of medicine preparation. The technology comprises the following steps: raw material processing, ultrasonic enzymatic hydrolysis extraction, ion exchange adsorption, heparin elution and ethanol precipitation. The technology has the advantages of shortening of the production time of crude heparin sodium, increase of the production efficiency, increase of the yield of the crude heparin sodium, and suitableness for industrial promotion and production.

The invention discloses a bacteriostatic chitin enzymolysis method which comprises the following steps: 1, preparation of chitinase through microbial fermentation: by taking a chitinase preparation microorganism as a test bacterium, fermenting to prepare chitinase, obtaining chitinase crude enzyme after the fermentation is finished, and purifying, dialyzing and concentrating to obtain chitin degrading enzyme; 2, enzymolysis of chitin: putting the chitin degrading enzyme in a reaction kettle, adding chitin powder, a metal ion activating agent, an auxiliary enzyme preparation and an organic reagent, and heating at constant temperature and stirring to obtain enzymatic hydrolysate when the chitin powder is completely degraded; and 3, recovery of product: centrifuging the enzymatic hydrolysate to obtain a biphasic system, wherein the upper system is an organic system and the lower system is a water system, adsorbing by adsorption resins, eluting, collecting the eluent and treating the eluent to obtain the product. According to the bacteriostatic chitin enzymolysis method, the raw materials can be repeatedly utilized and the conversion process is sterile so that high yield can be obtained when the energy consumption is reduced; and the degraded product is favorable in biological activity so that remarkable economic benefit is provided.

The invention discloses a method for preparing feed nucleotide by waste beer yeast. The method includes preparing the waste beer yeast into 5'-nucleotide by wall-breaking, extracting, enzymolysis and the like. The content of RNA (ribonucleic acid) in extract is measured by ultraviolet spectroscopy. According to the extracted RNA content, the amount of 5'-phosphodiesterase to add is determined by calculating 2500-5000 active units of 5'-phosphodiesterase required per g of RNA substrate, and the RNA is enzymatically hydrolyzed into 5'-nucleotide. The method has the advantages that production cycle is short, investment is low, cost is low, product yield is high, the quality is stable, waste is turned into wealth, no pollutant is discharged and the like, and high economic and social values are achieved.

The invention discloses a process for extracting picroside from rhizoma picrorhizae through fermentation. The process comprises the following steps: dispersing cellulase, hemicellulase, protease, alpha-amylase and pectinase in water to obtain an enzyme preparation; adding sucrose ester and ammonium oxalate into the enzyme preparation, uniformly mixing, adding crushed rhizoma picrorhizae, stirringat the temperature of 25-35 DEG C for 4-10 hours at a stirring speed of 60-80 r / min, and inactivating at a high temperature to obtain a fermentation product; feeding the fermentation product into an ethanol solution, standing at normal temperature for 20-30 hours, carrying out ultrasonic treatment for 20-30 minutes at the ultrasonic frequency of 30-40 kHz, heating, refluxing and extracting for 4-6hours, filtering, decompressing filtrate, recovering a solvent, concentrating, loading onto a polyamide chromatographic column, washing the polyamide column with water until the polyamide column is colorless, and eluting with a mixed solvent of which the volume is 3 times that of the column bed; and collecting the eluate, concentrating and drying to obtain the picroside.

The invention relates to a herbicide containing peach peel fermented vinegar, and belongs to the technical field of pesticide. The herbicide is prepared via following steps: yellow peach peel is subjected to first fermentation and second fermentation so as to obtain yellow peach peel fermented vinegar; the yellow peach peel fermented vinegar is mixed with saponin gum, 3 to 5 parts of Karaya gum, and 2 to 4 parts of seconary alkane sulphonate sodium; and an obtained mixture is stirred to be uniform. According to the invention, yellow peach peel obtained in juice production or can production process is taken as a raw material, fermentation is adopted, and the herbicide is prepared via combination with other raw materials; the herbicide is friendly to the environment, is safe to crops, and possesses excellent prevention effects on weed; and in addition, recycling of yellow peach peel is realized, and utilization value of yellow peach peel is increased.

The invention relates to the field of historical relic detection, and discloses a preparation method of a gossypin enzymatic hydrolysis product coupled protein used for historical relic detection. The method comprises the following steps: degreasing gossypin, processing the degreased gossypin with acetic acid and sodium hydroxide to remove lignin and hemicellulose, carrying out enzymatic hydrolysis on the obtained cellulose by using cellulase and beta-glucosidase, preparing a deionized water-ammonium sulfate-polyethylene glycol-400 extract liquid, respectively freeze-drying an upper layer liquid and a lower layer liquid which are obtained after extraction to respectively obtain a recovered enzyme and glucose powder, dissolving the glucose powder and bovine serum albumin in deionized water and a potassium bromide solution, standing the obtained solution in a constant temperature and constant humidity box, and freeze-drying the solution to obtain a glucose-bovine serum albumin conjugate. Coupled macromolecules obtained through using the method are stable, and can be used to inoculate animals as a complete antigen to obtain a corresponding specific antibody, so feasibility is provided for the identification of cotton fabrics in ancient textile historical relics.

The invention belongs to the technical field of sewage processing, and more specifically relates to a method used for livestock and poultry sewage processing. The method comprises following steps: precipitating agent precipitation treatment, composite enzyme enzymatic hydrolysis, microbiological culture media microbes are increased and microbiological degradation is carried out, and refreshing agent deodorizing sterilization processing. The method is capable of reducing pollutants such as ammonia nitrogen and phosphor in sewage, reaching obvious sterilizing, deodorizing, nitrogen removing, phosphate removing, and decolouring effects, is excellent in sewage processing effect, high in removing rate, is simple in operation, is safe, and is friendly to the environment.

The invention discloses a glycated serum protein determination kit. The kit contains a reagent R1 and a reagent R2; the reagent R1 contains Tris buffer solution, trypsin, peroxidase, an anti-interference substance, a protein denaturant, an ionic liquid, CaCl2, a surfactant and a preservative; and the reagent 2 contains the Tris buffer solution, fructosaminase, 4-aminoantipyrine, sodium 3-(N-ethyl-3-methylanilino)-2-hydroxypropanesulfonate (TOOS), a surfactant, a stabilizer and a preservative. The kit disclosed by the invention is a liquid kit high in accuracy and good in stability. The invention also discloses a preparation method and application of the kit.

The invention relates to the field of historical relic detection, and discloses a preparation method of a gossypin enzymatic hydrolysis product coupled protein used for historical relic detection. The method comprises the following steps: degreasing gossypin, processing the degreased gossypin with acetic acid and sodium hydroxide to remove lignin and hemicellulose, carrying out enzymatic hydrolysis on the obtained cellulose by using cellulase and beta-glucosidase, preparing a deionized water-ammonium sulfate-polyethylene glycol-400 extract liquid, respectively freeze-drying an upper layer liquid and a lower layer liquid which are obtained after extraction to respectively obtain a recovered enzyme and glucose powder, dissolving the glucose powder and bovine serum albumin in deionized water and a potassium bromide solution, standing the obtained solution in a constant temperature and constant humidity box, and freeze-drying the solution to obtain a glucose-bovine serum albumin conjugate. Coupled macromolecules obtained through using the method are stable, and can be used to inoculate animals as a complete antigen to obtain a corresponding specific antibody, so feasibility is provided for the identification of cotton fabrics in ancient textile historical relics.

The invention discloses a method using Spanish mackerel to cooperatively produce cartilage polysaccharide and bone collagen active peptide. The method includes the following steps: 1) drying and crushing Spanish mackerel bones after cleaning; 2) placing bone powder under far-infrared rays to perform irradiation; 3) performing hot press extraction after mixing the bone powder and water; 4) using compound proteinase to perform enzymolysis; and 5) performing ultrafiltration and separation, and respectively obtaining cartilage polysaccharide and bone collagen active peptide. The method uses the far-infrared rays to make organism cells being at the highest vibration energy level, thus, biomacromolecule in the bone powder can be extracted and decomposed more easily during the follow-up hot pressextraction and enzymolysis, so that the cartilage polysaccharide and bone collagen active peptide with higher additional values can be decomposed and generated while the speed of enzymolysis processes are greatly accelerated.

The present invention discloses a compound based on active polypeptides of sea bass fins. The compound comprises the following ingredients in parts by weight: 3-6 parts of active polypeptides of sea bass fins, 18-24 parts of collagen, 5-11 parts of polysaccharides of cistanche deserticola, 1-4 parts of lycium barbarum polysaccharides, 5-11 parts of hydroxymethyl cellulose, 0.1-0.3 part of trace element, 0.8-1.4 parts of vitamin E, 4-8 parts of fruit powder and 38-45 parts of water. Beneficial effects are as follows: the compound is rich and comprehensive in nutrition, easy for absorption, free of smelly and bitter tastes, refreshing and smooth in mouthfeel, and free of chemical preservatives and toxic and side effects, can be drunk by any consumers, can remove free radicals in body, reduces attacks of the free radicals on the body, can activate activities of superoxide dismutase, strengthens too much decomposition of peroxide, and thus plays functions of protecting and preventing aging of the body.