The invention belongs to the technical field of
glucan, and particularly relates to a green preparation method of
yeast glucan. The green preparation method includes the steps: 1 adding common
yeast cell walls into
distilled water, uniformly stirring the common
yeast cell walls and the
distilled water to form suspension liquid, heating the suspension liquid at constant temperature for 2-4 hours, adding
tourmaline and
hydrogen peroxide, performing sealed cooking for 2-4 hours, keeping the temperature for 2-6 hours, taking out the
tourmaline and performing
centrifugation to obtain precipitates;2 adding the precipitates into the
distilled water, uniformly stirring the precipitates and the distilled water, adding dispersing agents, heating and stirring the dispersing agents and the distilledwater to form turbid liquid, adding alkaline liquid, and adjusting pH (potential of
hydrogen) to be 9-11 to obtain alkaline turbid liquid; 3 adding
protease and the
tourmaline into the alkaline turbidliquid, performing constant-temperature enzymolysis for 4-6 hours, taking out the tourmaline and then performing
centrifugation and washing to obtain insoluble substances; 4 adding the insoluble substances into
acetone, performing
microwave reaction for 2-5 hours, and filtering and freeze-
drying reactants to obtain yeast
glucan powder. The yeast
cell walls serve as production raw materials, the tourmaline serves as an auxiliary catalyst and an auxiliary oxidizer, and the yield of the yeast glucan is greatly improved.