Glycated serum protein determination kit as well as preparation method and application thereof

Abstract

The invention discloses a glycated serum protein determination kit. The kit contains a reagent R1 and a reagent R2; the reagent R1 contains Tris buffer solution, trypsin, peroxidase, an anti-interference substance, a protein denaturant, an ionic liquid, CaCl2, a surfactant and a preservative; and the reagent 2 contains the Tris buffer solution, fructosaminase, 4-aminoantipyrine, sodium 3-(N-ethyl-3-methylanilino)-2-hydroxypropanesulfonate (TOOS), a surfactant, a stabilizer and a preservative. The kit disclosed by the invention is a liquid kit high in accuracy and good in stability. The invention also discloses a preparation method and application of the kit.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a test kit for glycosylated serum protein, and also relates to a preparation method and application of the test kit for glycosylated serum protein. Background technique [0002] Glycated serum protein is a non-enzymatic glycation reaction between serum glucose and the N-terminal amino group of albumin and other serum protein molecules to form a polymer ketamine structure. The binding process of various serum proteins and sugar is basically the same. The non-ionic ε or α-amino group on the protein molecule and the carboxyl group on the aldose form an unstable compound, namely Schiff's base, which can be dissociated It is a protein and aldose, and can be rearranged through Amadori transposition to generate a relatively stable amino-1-deoxy-2-ketose compound, called ketosamine (Ketosamine), because its structure is similar to fructosamine (Fructosamine, FMN) Therefore, the ...

AI Technical Summary

Problems solved by technology

Affinity chromatography is time consuming and difficult to automate

The NBT method is a commonly used quantitative method for glycated serum protein in China. Nitrotetrazolium blue is selected as the chromogen in the NBT method, and the colorimetric method is used for the determination of glycated serum protein. This method is fast and sensitive, but the stability of the kit is poor, and NBT is water-soluble Not good, it is very easy to adsorb on the cuvette and pipeline of the biochemical analyzer, causing pollution to the analyzer

The protease method has the advantages of good specificity and no pollution to biochemical analyzers, but the reagents rely on imports, and the reagents are generally freeze-dried and expensive

If a conventional protease system is used for the enzymatic hydrolysis reaction of glycated serum protein, it will take tens of minutes or even several hours to complete the enzymatic hydrolysis, and the enzymatic hydrolysis method simply increasing the concentration of protease greatly increases the reaction cost

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