A serum sialic acid assay kit with stability and strong anti-interference ability and its preparation method and application

Abstract

The invention provides a sialic acid detection kit. The kit contains a reagent R1 and a reagent R2; the reagent R1 contains the following components: a tris(hydroxymethyl)methyl aminomethane-hydrochloric acid (Tris-HCL) buffer solution, lactic dehydrogenase (LDH), N-acetylneuraminic acid aldolase, a reduced coenzyme I (NADH), a surfactant, a stabilizer, EDTA-Na2 and a preservative; and the reagentR2 contains the following components: a tris(hydroxymethyl)methyl aminomethane-hydrochloric acid (Tris-HCL) buffer solution, neuraminidase, a surfactant, a stabilizer and a preservative. A productionmethod and application of the kit are provided at the same time. The kit is a liquid kit with high stability, high anti-interference capability, high accuracy and good repeatability.

Description

technical field [0001] The invention relates to the technical field of biochemical reagent determination, in particular to a sialic acid determination kit, and also relates to a preparation method and application of the sialic acid determination kit. Background technique [0002] Sialic acid (Sialic acid SA), also known as N-acetylneuraminic acid, is a general term for a family of compounds containing 9-carbon sugar neuraminic acid, and its main derivative in human tissues is N-acetylneuraminic acid. It is located at the end of glycoprotein and glycolipid structure, and glycoprotein and glycolipid are important components of cell membrane, so sialic acid plays a decisive role in maintaining the function of cell surface. Such as participating in the recognition of the reaction between the host and the disease body, protecting the membrane from proteolysis, and contributing to the activation of hormone receptors, etc. In recent years, many scholars have quantitatively measure...

AI Technical Summary

Problems solved by technology

Thiobarbituric acid chromogenic method and periodic acid-resorcinol method are the original chemical detection methods, but the two methods have poor anti-interference ability and cumbersome operation

Enzyme-linked immunoassay has high sensitivity and specificity, but there are many interference factors, poor repeatability, and high price; fluorescence analysis and high-performance liquid chromatography have high accuracy and sensitivity, but the equipment is expensive and the reagents are inconvenient to store. And it is manual operation, which takes a long time and is expensive, so it cannot be applied in clinical practice; the neuraminidase method has the advantages of high specificity, simple and quick operation, accurate and safe, automatic analysis, and low cost. There are shortcomings such as poor stability and repeatability of the domestic kits in the art, and the present invention improves the shortcomings of the existing sialic acid kits to meet the requirements of clinical testing and chemical analysis

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