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An anti-interference and stable serum total bilirubin (enzymatic method) assay kit and its preparation method and application

A technology of total bilirubin and bilirubin oxidase, which is applied in the field of biochemical reagent determination, can solve the problems of reagents that cannot be stabilized for a long time, are easily affected by blood lipids, and have poor anti-interference ability, and achieve enhanced anti-interference ability and excellent performance indicators , excellent effect of stability

Active Publication Date: 2021-11-23
中拓生物有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The diazonium salt method is widely used in clinical laboratories to detect bilirubin, but the method is susceptible to interference from many factors, and the reagents cannot be stable for a long time, which is affected to a certain extent in disease diagnosis
The vanadic acid oxidation method is easy to contaminate the instrument and affect other tests
The oxidase method has high specificity and high accuracy, but existing kits have shortcomings such as poor anti-interference ability, poor stability, and being easily affected by blood lipids. The present invention improves the shortcomings of the existing total bilirubin kit to meet Requirements for clinical testing and chemical analysis

Method used

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  • An anti-interference and stable serum total bilirubin (enzymatic method) assay kit and its preparation method and application
  • An anti-interference and stable serum total bilirubin (enzymatic method) assay kit and its preparation method and application
  • An anti-interference and stable serum total bilirubin (enzymatic method) assay kit and its preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0050] A conventional total bilirubin assay kit includes reagent R1 and reagent R2.

[0051] Reagent R1 contains the following components:

[0052] Piperazine-1,4-diethanesulfonic acid (PIPES) buffer solution 50mmol / L;

[0053] NaCl 2g / L;

[0054] SDS 1g / L;

[0055] Sodium cholate 1g / L;

[0056] Citric acid 0.1g / L;

[0057] Sorbitan Laurate 1%;

[0058] Polyoxypropylene Mannitol Dioleate 1%;

[0059] proclin300 1 ml / L.

[0060] Reagent R1 has a pH value of 8.2.

[0061] Reagent R2 contains the following ingredients:

[0062] Piperazine-1,4-diethanesulfonic acid (PIPES) buffer solution 50mmol / L;

[0063] Bovine serum albumin (BSA) 5g / L;

[0064] Mannitol 10g / L;

[0065] Triton 100 1%;

[0066] Bilirubin oxidase 1KU / L;

[0067] proclin300 1ml / L.

[0068] Reagent R2 has a pH of 9.0.

[0069] Wherein said percentage is volume ratio.

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Abstract

The invention provides a total bilirubin (TB) assay kit, the kit contains reagent R1 and reagent R2; reagent R1 contains the following components: tris-hydrochloric acid buffer, NaCl, sodium cholate, lemon Acids, surfactants, preservatives. Reagent R2: tris-hydrochloric acid buffer, bilirubin oxidase, stabilizer, preservative. The invention also provides the preparation method and application of the kit. The kit uses different types of stabilizers and surfactants, which can effectively avoid the interference of blood lipids. It has strong anti-interference ability, high sensitivity and good stability. Low cost liquid kit.

Description

technical field [0001] The invention relates to the technical field of biochemical reagent determination, in particular to a total bilirubin determination kit, and also relates to a preparation method and application of the total bilirubin determination kit. Background technique [0002] Bilirubin is a type of bile pigment, the main pigment in human bile. Bilirubin is the main metabolite of iron porphyrin compounds in the body. It is toxic and can cause irreversible damage to the brain and nervous system, but it also has an antioxidant function and can inhibit the oxidation of linoleic acid and phospholipids. Bilirubin is an important basis for judging jaundice clinically, and it is also an important indicator of liver function. [0003] Total bilirubin: High indirect bilirubin and high direct bilirubin indicate hepatocellular jaundice, damage to liver cells, decreased liver function, and the liver cannot completely convert indirect bilirubin into direct bilirubin. The com...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/72C12Q1/26G01N33/52
CPCC12Q1/26G01N33/52G01N33/728
Inventor 刘安娜
Owner 中拓生物有限公司
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