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47 results about "Bilirubin oxidase" patented technology
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In enzymology, a bilirubin oxidase (EC 1.3.3.5) is an enzyme encoded by a gene in various organisms that catalyzes the chemical reaction 2 bilirubin + O₂ ⇌ 2 biliverdin + 2 H₂O Thus, the two substrates of this enzyme are bilirubin and O₂, whereas its two products are biliverdin and H₂O. This enzyme belongs to the family of oxidoreductases, to be specific those acting on the CH-CH group of donor with oxygen as acceptor.
Miniature biological fuel cell that is operational under physiological conditions, and associated devices and methods
InactiveUS7368190B2Reduce physical sizeReduced dimensionMicrobiological testing/measurementVolume/mass flow measurementOperabilityRedox polymers
A fuel cell is provided with an anode and a cathode. The anode is in electrical communication with an anode enzyme and the cathode is in electrical communication with a cathode enzyme. The anode enzyme is preferably an oxidase or a dehydrogenase. The cathode enzyme is a copper-containing enzyme, such as a laccase, an ascorbate oxidase, a ceruloplasmine, or a bilirubin oxidase. Preferably, the cathode enzyme is operable under physiological conditions. Redox polymers serve to wire the anode enzyme to the anode and the cathode enzyme to the cathode. The fuel cell can be very small in size because it does not require a membrane, seal, or case. The fuel cell can be used in connection with a biological system, such as a human, as it may operate at physiological conditions. By virtue of its size and operability at physiological conditions, the fuel cell is of particular interest for applications calling for a power source implanted in a human body, such as a variety of medical applications.
Owner:ABBOTT DIABETES CARE INC
Miniature biological fuel cell that is operational under physiological conditions, and associated devices and methods
InactiveUS20080044721A1Reduce physical sizeReduced dimensionMicrobiological testing/measurementVolume/mass flow measurementOperabilityRedox polymers
A fuel cell is provided with an anode and a cathode. The anode is in electrical communication with an anode enzyme and the cathode is in electrical communication with a cathode enzyme. The anode enzyme is preferably an oxidase or a dehydrogenase. The cathode enzyme is a copper-containing enzyme, such as a laccase, an ascorbate oxidase, a ceruloplasmine, or a bilirubin oxidase. Preferably, the cathode enzyme is operable under physiological conditions. Redox polymers serve to wire the anode enzyme to the anode and the cathode enzyme to the cathode. The fuel cell can be very small in size because it does not require a membrane, seal, or case. The fuel cell can be used in connection with a biological system, such as a human, as it may operate at physiological conditions. By virtue of its size and operability at physiological conditions, the fuel cell is of particular interest for applications calling for a power source implanted in a human body, such as a variety of medical applications.
Owner:ABBOTT DIABETES CARE INC
Device for the determination of glycated hemoglobin
InactiveUS20060205029A1Retain and improve accuracySpeed the ultimate determinationMicrobiological testing/measurementVolume/mass flow measurementTotal hemoglobinCathode reaction
A method of determining the percentage of glycated hemoglobin in a blood sample is disclosed wherein at least one of the assay steps is performed electrochemically. The method includes determining the total amount of hemoglobin in a sample by electrochemically measuring, in an oxygen electroreduction reaction at a cathode, the amount of oxygen in the sample. Because the amount of oxygen dissolved in the sample is known, the total hemoglobin is determined by subtracting the amount of free oxygen from the total oxygen measured, recognizing the fast equilibrium Hb+O2⇄HbO2. This can be followed by determining the amount of glycated hemoglobin in the sample. The cathode reaction is accomplished by contacting the sample with an enzyme, the enzyme being a copper-containing enzyme having four copper ions per active unit. The family of these enzymes includes, for example, laccases and bilirubin oxidases. A device associated with such a process or method is also provided.
Owner:ABBOTT DIABETES CARE INC
Construction method and application of surface protein-embossed self-energized biological fuel cell sensor
InactiveCN106525943ARealize specific identification detectionEasy to operateMaterial analysis by electric/magnetic meansExternal energyCarbon nanotube
The invention discloses a construction method and application of a surface protein-embossed self-energized biological fuel cell sensor. The method comprises the steps that the surface of a carbon electrode is coated with a molecularly-imprinted polymer, specific protein is labeled with a phenylboronic acid-bilirubin oxidase-carbon nanotube nanocomposite after being adsorbed to the surface of the molecularly-imprinted polymer, and then a surface protein-embossed biological cathode is obtained; the surface of a carbon electrode is modified with a thionine-graphene-glucose dehydrogenase compound, and then a biological anode is obtained; the surface protein-embossed biological cathode, the biological anode and parts including a PMDS electrolytic tank and external resistors are assembled, and then the biological fuel cell sensor is obtained. According to the sensor, the high selectivity and sensitivity to specific glycoprotein are achieved in a compound system where multiple proteins exist to generate interference, external energy supply is not needed during molecular recognition, and large-scale production and application requirements are met.
Owner:CENT SOUTH UNIV
Stable serum 5'-ribotide hydrolase detection reagent with strong anti-interference capability and detection method
ActiveCN105420345AImprove stabilityAvoid turbidityMicrobiological testing/measurementBetaineSodium phosphates
The invention relates to the technical field of serum 5'-ribotide hydrolase detection, in particular to a serum 5'-ribotide hydrolase detection reagent. A PIPES buffer solution is adopted, and various stabilizers are added to remarkably improve the stability of the reagent; beta-sodium glycerophosphate, bilirubin oxidase and ascorbic acid oxidase are added to effectively prevent interference caused by alkaline phosphatase, cholerythrin and ascorbic acid and greatly enhance the anti-interference capability of the reagent. Besides, a preferred novel amphoteric surfactant, dodecyl dimethyl betaine (BS-12), is added to prevent a reaction system from turbidity, enhance the stability of a substrate and improve the anti-interference capability of the reagent.
Owner:BIOBASE BIODUSTRY (SHANDONG) CO LTD
Stable uric acid reagent with high anti-interference capacity and detection method
InactiveCN106290323AImprove stabilityImprove anti-interference abilityMaterial analysis by observing effect on chemical indicatorSucroseAlkylphenol
The invention relates to the technical field of uric acid detection, in particular to a uric acid detection reagent. A reagent R1 is prepared from a buffer solution, 4-aminoantipyrine, BSA, sucrose, trehalose, peroxidase, ascorbic acid oxidase, bilirubin oxidase, alkylphenol ethoxylates (APEO) and a preservative; a reagent R2 is prepared from a buffer solution, TOOS uricase.BSA sucrose.trehalose alkylphenol ethoxylates (APEO) and a preservative. The PIPES (piperazine-1,4-bisethanesulfonic acid) solution is adopted, the stabilizer BSA, sucrose and trehalose are added, and the stability of the reagent is greatly improved; the novel surfactant alkylphenol ethoxylates (APEO) is adopted, and therefore not only is the determination property of the product significantly improved, but also the stability and anti-interference capacity of the reagent are improved.
Owner:章丘美高义医疗器械有限公司
Stable high-interference-resistance direct bilirubin (oxidase method) detection reagent and detection method
InactiveCN109991177AImprove stabilityAvoid interferenceColor/spectral properties measurementsSucroseTurbidity
The invention relates to the field of serum direct bilirubin detection technologies, in particular to a serum direct bilirubin (oxidase method) detection reagent. A reagent R1 contains a buffer solution, sodium fluoride, NAC(N-acetylcysteine), sodium 4-methylbenzenesulfonate, lipase, ascorbic acid oxidase, alpha-cyclodextrin, sucrose, trehalose, PEG-6000, fatty alcohol polyoxyethylene ether (AEO-9) and a preservative; and a reagent R2 contains a buffer solution, bilirubin oxidase, sucrose, trehalose, PEG-6000, fatty alcohol polyoxyethylene ether (AEO-9) and a preservative. Through the reagent,the specificity and accuracy of direct bilirubin testing are obviously improved, turbidity of a reaction system can be prevented, a reaction is promoted, and the stability and interference resistanceof the reagent are further improved.
Owner:济南宇鑫生物科技有限公司
Test paper for detecting uric acid content range in urine
InactiveCN106404758ALow costEasy to carryMaterial analysis by observing effect on chemical indicatorFiberColor reaction
The present invention relates to a test paper strip for detecting the uric acid concentration in urine by using a color reaction. According to the present invention, an enzyme coloring agent, bilirubin oxidase and bamboo fibers are adhered on a test paper substrate, and the main components of the enzyme coloring agent of the test paper strip comprise uricase, horseradish peroxidase, and a coloring agent.
Owner:ZHUHAI HEFAN MEDICINE
High-efficiency enzymatic biological fuel battery cathode and preparation method thereof
ActiveCN101931083AEnables direct electrochemicalGood catalyticCell electrodesBiochemical fuel cellsCross-linkFuel cells
The invention discloses a high-efficiency enzymatic biological fuel battery cathode and a preparation method thereof. The biological fuel battery cathode comprises a substrate electrode, a carbon nano-material layer coated on the substrate electrode and an enzymatic layer coated on the carbon nano-material layer, wherein the enzymatic layer comprises the following a material a) and b): a) laccase or bilirubin oxidase, b) the laccase or the bilirubin oxidase, which is cross-linked by a cross linking agent. Compared with an ordinary enzymatic biological fuel battery cathode, the enzymatic biological fuel battery cathode of the invention has higher oxygen catalytic reduction efficiency.
Owner:INST OF CHEM CHINESE ACAD OF SCI
A stable serum phospholipid detecting reagent high in interference-resisting capability and a detecting method
ActiveCN105543336AAvoid interferenceImprove anti-interference abilityMicrobiological testing/measurementGlycerolTurbidity
The invention relates to the technical field of serum phospholipid detection and particularly relates to a serum phospholipid detecting reagent. A reagent R1 comprises a buffer solution, phospholipase D, DAOS, ascorbic acid oxidase, bilirubin oxidase, polyethylene glycol 6000, cane sugar, xanthan gum, mannitol, trehalose, BSA, glycerol propoxylate-block-ethoxylate (Pluranic L64) and an aseptic. A reagent R2 comprises the buffer solution, 4-aminoantipyrine, peroxidase, choline oxidase, the polyethylene glycol 6000, the cane sugar, the xanthan gum, the mannitol, the trehalose, the BSA, the glycerol propoxylate-block-ethoxylate (Pluranic L64) and the aseptic. The HEPES buffer solutions and a novel Trinder reaction chromogen DAOS are adopted. A plurality of stabilizers are added to obviously improve stability of the detecting reagent. The bilirubin oxidase and the ascorbic acid oxidase are added, thus effectively avoiding interference caused by bilirubin and ascorbic acid and greatly improving interference-resisting capability of the detecting reagent. In addition, addition of the glycerol propoxylate-block-ethoxylate (Pluranic L64) which is an optional nonionic surfactant prevents reaction system turbidity, enhances substrate stability and improves the interference-resisting capability of the detecting reagent.
Owner:BIOBASE BIODUSTRY (SHANDONG) CO LTD
Total cholesterol detection reagent with high accuracy and disturbance resisting capacity
ActiveCN105385751AWill not negatively affectGuaranteed cushioning effectMicrobiological testing/measurementCholesterolActive agent
The invention provides a total cholesterol detection reagent with high accuracy and disturbance resisting capacity and relates to the technical field of total cholesterol detection. A biological buffer CAPS (3-(hexanaphthene amino)-1-propanesulfonic acid) is used in the reagent R, and therefore the buffering ability is ensured; a surfactant octadecy trimethyl ammonium bromide and a surfactant EMULGEN-707 are added into the reagent R, the emulsification effect of the reagent is improved well, and the accuracy and specificity of the reagent are improved; stabilizers such as trehalose and mannitol are added into the reagent R, and the stability of the reagent can be improved effectively; hydroxy ethidene diphosphonic acid is added into the reagent R, heavy metal ions can be effectively chelated, and the accuracy of the reagent can be improved; chemical-resistant succinoxidase and bilrubin oxidase are added into the reagent, disturbance of ascorbic acid and bilirubin can be effectively removed, and the reagent is simple in preparation, low in price and very suitable for large-area clinical popularization.
Owner:BIOBASE BIODUSTRY (SHANDONG) CO LTD
Method for assaying bilirubin and assay instrument used in bilirubin assay
ActiveUS20100323382A1Efficient responseShorten the timeBioreactor/fermenter combinationsBiological substance pretreatmentsAssaySURFACTANT BLEND
A method for assaying bilirubin in which a dry reagent is used, which is capable of accelerating the reaction of bilirubin oxidase, and an assay instrument to be used in the method for assaying bilirubin, are provided. The method for assaying bilirubin is characterized in that a biological sample and a bilirubin oxidase-containing dry reagent are mixed first, and then the mixture obtained and a surfactant-containing dry reagent are mixed. An assay instrument (1) to be used in bilirubin assay is characterized in that a bilirubin oxidase and a surfactant are arranged in any of a sample supply part (11), passages (12, 13, 14), and a detection part (15) in a manner such that the bilirubin oxidase is positioned closer to the sample supply part (11) than the surfactant is.
Owner:ARKRAY INC
Stable and high-interference resistance serum adenosine deaminase detection reagent and detection method
InactiveCN106119339AAvoid interferenceImprove anti-interference abilityMicrobiological testing/measurementPolyethylene glycolAscorbate Oxidase
The invention relates to the technical field of detection of serum adenosine deaminase and in particular relates to a serum adenosine deaminase detection reagent. R1 comprises buffer solution, 4-aminoantipyrine, PNP, XOD, POD, bilirubin oxidase, ascorbic acid oxidase, glycerin, polyethylene glycol 6000, ethylene glycol, mannitol, mycose, BSA, alkyl glycoside and preservatives; R2 comprises buffer solution, adenosine, 3-hydroxy-2,4,6-triiodobenzoic acid, glycerin, polyethylene glycol 6000, ethylene glycol, mannitol, mycose, BSA, alkyl glycoside and preservatives. With the adoption of the novel Trinder reaction chromogen substance 3-hydroxy-2,4,6-triiodobenzoic acid, multiple stabilizers are added, and the stability of the reagent is obviously improved; and due to the added bilirubin oxidase, ascorbic acid oxidase and novel nonionic surfactant alkyl glycoside (APG), interference of bilirubin and ascorbic acid is avoided, a turbid reaction system is prevented, and the substrate stability and the interference resistance of the reagent are enhanced.
Owner:BIOBASE BIODUSTRY (SHANDONG) CO LTD
Stable total bilirubin (oxidase method) detection reagent with strong anti-interference ability and detection method
InactiveCN110274881AAvoid interferenceImprove anti-interference abilityColor/spectral properties measurementsBiological testingLipid formationSucrose
The invention relates to the technical field of total bilirubin detection, especially to a serum total bilirubin (oxidase method) detection reagent. A reagent R1 contains a buffer solution, lipase, ascorbic acid oxidase, alpha-cyclodextrin, sucrose, trehalose, PEG-6000, castor oil polyoxyethylene ether (EL-20) and a preservative. A reagent R2 contains a buffer solution, bilirubin oxidase, sucrose, trehalose, PEG-6000, castor oil polyoxyethylene ether (EL-20) and a preservative. The interference of lipid turbidity and ascorbic acid oxidase can be effectively avoided and the anti-interference ability of the reagent can be greatly enhanced by optimizing a reaction system and adding the lipase, the alpha-cyclodextrin and the ascorbic acid oxidase. The adoption of the novel PIPES(piperazine-1,4-diethylsulfonic acid) buffer solution and the addition of stabilizers such as the sucrose, the trehalose, the polyethylene glycol 6000 can effectively improve the stability of the reagent. Moreover, the addition of the optimal novel non-ionic surfactant castor oil polyoxyethylene ether (EL-20) can prevent the reaction system from turbidity and further improve the stability and the anti-interference ability of the reagent.
Owner:济南煊赫生物科技有限公司
Stable adenosine deaminase reagent high in anti-interference capability and detection method
InactiveCN105238847AImprove stabilitySuitable for automatic analysisMicrobiological testing/measurementSucrosePeroxidase
The invention relates to the technical field of adenosine deaminase detection, in particular to an adenosine deaminase detection reagent. A reagent R1 comprises a buffer solution, 4-aminoantipyrine, BSA, cane sugar, trehalose, peroxidase, ascorbic acid oxidase, bilirubin oxidase and preservatives; a reagent R2 comprises a buffer solution, adenosine, EHSPT, BSA, cane sugar and preservatives. The adenosine deaminase detection reagent has the advantages that the phosphate buffer solution is adopted, the stabilizer BSA, the cane sugar and the trehalose are added, and accordingly, stability of the reagent is improved greatly; determination performance is improved remarkably, and stability and anti-interference capability of the reagent are enhanced.
Owner:BIOBASE BIODUSTRY (SHANDONG) CO LTD
Creatininase method detection kit
InactiveCN109406798AHigh sensitivityImprove accuracyDisease diagnosisBiological testingBiotechnologyCreatininase
The invention discloses a creatininase method detection kit. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 is prepared from a buffer solution, creatinase, sarcosine oxidase,ascorbic acid oxidase, bilirubin oxidase, TOOS, peroxidase, a stabilizer, and a surfactant; the reagent R2 is prepared from a buffer solution, 4-aminoantipyrine, creatininase, a stabilizer, a surfactant, and 0.1 to 10g / L anti-bacterial preservative. The creatininase method detection kit has the advantages of lowering the toxicity effectively, lowering the environment pollution, preventing the drugtolerance of bacteria, preventing super bacteria from generating and lowering the human health risk, along with high stability, and can be applied to creatinine detection of chronic disease management.
Owner:NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
Biological photovoltaic cell and preparation method thereof
ActiveCN111081476AIncrease energy densityImprove photoelectric response performanceLight-sensitive devicesBiochemical fuel cellsStructural unitBattery cell
The invention relates to the field of biological photovoltaic cells, and discloses a biological photovoltaic cell and a preparation method thereof. The biological photovoltaic cell provided by the invention contains a polymer formed by a structural unit as shown in a formula (1) and a capsule-like body; the number-average molecular weight of the polymer is 8 *10 < 3 >-2*10 < 4 >; the distributionindex of the polymer ranges from 1 to 2; and the cathode of the biological photovoltaic cell contains bilirubin oxidase. According to the biological photovoltaic cell, the anode containing the polymerand the capsule-like body and the cathode containing the bilirubin oxidase are matched with each other so as to be used, so that the energy density and photoelectric response performance of the biological photovoltaic cell can be greatly improved, and photoelectric conversion efficiency is improved.
Owner:INST OF CHEM CHINESE ACAD OF SCI
Bilirubin electrochemical transducer and preparation method thereof
InactiveCN104792832AImprove accuracyImprove anti-interference abilityMaterial electrochemical variablesOxidation stateTransducer
The invention relates to the technical field of electrochemical detection, in particular to a bilirubin electrochemical transducer and a preparation method thereof. The bilirubin electrochemical transducer comprises an electrode, a bilirubin oxidase layer and an anti-interference enzyme layer, wherein the bilirubin oxidase layer is arranged between the electrode and the anti-interference enzyme layer, and comprises bilirubin oxidase and an oxidation-state electronic mediator; the anti-interference enzyme layer comprises ascorbic acid oxidase. The bilirubin electrochemical transducer has the advantages of being high in sensitivity, wide in linearity range, low in sample demand, high in anti-interference capacity, and short in detection time.
Owner:SINOCARE
Glutathione reductase detection kit as well as preparation method and application thereof
PendingCN113528609AReduce descent speedStable pH environmentMicrobiological testing/measurementActive agentPotassium ferricyanide
The invention provides a glutathione reductase detection kit and a preparation method and application thereof. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises a potassium phosphate buffer solution, EDTA, GSSG, ascorbic acid oxidase, bilirubin oxidase, potassium ferricyanide, a surfactant, a first bacteriostatic agent and a second bacteriostatic agent; and the reagent R2 comprises a sodium bicarbonate buffer solution, NADPH, the first bacteriostatic agent, a surfactant and a stabilizer. Compared with the prior art, the glutathione reductase detection kit provided by the invention has the advantages of good airborne stability, high sensitivity and strong antibacterial ability, and can meet requirements of clinical application of glutathione reductase detection.
Owner:NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
High-efficiency enzymatic biological fuel battery cathode and preparation method thereof
ActiveCN101931083BCatalytic reductionThe result is obviousCell electrodesBiochemical fuel cellsCross-linkFuel cells
Owner:INST OF CHEM CHINESE ACAD OF SCI
Method for detecting glucose by electrochemical/colorimetric dual-mode sensor
InactiveCN111796016AReduce experiment costQuick checkMaterial analysis by observing effect on chemical indicatorMaterial electrochemical variablesLaser cuttingElectrochemistry
The invention discloses a method for detecting glucose by using a paper-based electrochemical / colorimetric dual-mode sensor. A hydrophobic region and a hydrophilic region are prepared on paper by utilizing wax printing and laser cutting technologies, and a carbon electrode is printed by virtue of a silk-screen printing technology. Different areas of the paper chip are functionalized through different methods, visual pre-judgment of glucose can be achieved through the color development effect of 3, 3 ', 5, 5'-tetramethyl benzidine, and a simple, convenient and rapid method is provided. A biofuel cell is constructed by utilizing the specific catalysis of glucose oxidase to glucose and the reduction effect of bilirubin oxidase to oxygen, and the open-circuit voltage is recorded by virtue of an electrochemical workstation, so that the ultra-sensitive detection of glucose is realized.
Owner:UNIV OF JINAN
Accurate and efficient single agent low density lipoprotein cholesterol detecting reagent
InactiveCN109097436AMake sure to participate in the responseEnhanced inhibitory effectMicrobiological testing/measurementLow density lipoprotein cholesterolPeroxidase
The invention relates to the technical field of low density lipoprotein cholesterol detection, in particular to an accurate and efficient single agent low density lipoprotein cholesterol detecting reagent, wherein a reagent R comprises 50-100 mmol / L of buffer solution, 5g / L of magnesium chloride polyanion, 1-5 g / L of saccharide ring, 0.1-1 g / L of HDAOS (N-(2-hydroxy-3-sulfopropyl)-3,5-dimethyloxyaniline sodium salt), 0.1-1 g / L of surfactant 1, 0.1-1 g / L of surfactant 2, 5-10 g / L of surfactant 3, 1-5 KU / L of ascorbate oxidase, 1-3 KU / L of bilirubin oxidase, 1-3 KU / L of peroxidase, 0.1-0.3 g / L of 4-AA and 1-3 mL / L of preservative. The accurate and efficient single agent low density lipoprotein cholesterol detecting reagent has the advantages of being convenient, simple, accurate and efficient.
Owner:BIOBASE BIODUSTRY (SHANDONG) CO LTD
Solid fermentation method of bilirubin oxidase
InactiveCN101974496ARich sourcesReduce manufacturing costMicroorganism based processesOxidoreductasesSporeMyrothecium roridum
The invention discloses a solid fermentation method of bilirubin oxidase, which has the purposes of low cost, simple production process and convenient enzyme extraction. The solid fermentation method comprises the following steps of: (1) separating and screening myrothecium roridum from soil, and preparing a myrothecium roridum spore suspension; and (2) weighing 3-7 parts by weight of bran, placing the bran in a triangular flask, adding 13-17 parts by weight of distilled water, uniformly agitating the bran and the distilled water by using a glass tick, sterilizing under high pressure at 121-130 DEG C for 20-30 min, cooling, inoculating with 1-5 parts by weight of spore suspension prepared in the step (1), cultivating at 22-31 DEG C for 7-11 days, taking out the triangular flask, adding 50-70 parts by weight of distilled water into the triangular flask, leaching for 2-4 h through table concentrator oscillation, and filtering by using qualitative filter paper to obtain the bilirubin oxidase crude liquid. The invention has the advantages of abundant resources and easy acquisition of the raw materials, low production cost, simple, convenient and feasible enzyme extraction process and energy-saving and environmental-friendly production process.
Owner:XINXIANG MEDICAL UNIV
Alpha-hydroxybutyrate dehydrogenase detection reagent with good stability and high accuracy
InactiveCN105651763AImprove stabilityImprove anti-interference abilityMaterial analysis by observing effect on chemical indicatorSucroseEtidronic acid
Owner:BIOBASE BIODUSTRY (SHANDONG) CO LTD
Direct bilirubin assay method and kit
InactiveCN109541238AImprove the safety of useReduce pollutionColor/spectral properties measurementsBiological testingPhysical chemistryOxidative enzyme
The invention discloses a direct bilirubin assay method and a kit. The direct bilirubin assay method utilizes an oxidase and a substrate thereof to react to form hydrogen peroxide, and then hydrogen peroxide and peroxidase react to release oxygen. Direct bilirubin in a solution is oxidized to biliverdin, and the absorption peak is decreased at 450 nm. The degree of decrease is recorded to calculate the direct bilirubin content. According to the invention, another oxidase which is cheap, easy to obtain and stable and can generate hydrogen peroxide and the substrate thereof are used to replace direct bilirubin oxidase; the cost is greatly reduced compared with the cost of an original direct bilirubin oxidase assay method; the experimental data acquired by the detection method proves that thesensitivity, accuracy and repeatability of the analysis are excellent; and the detection reagent has the advantages of high use safety, low environmental pollution and good practicability.
Owner:武汉中太生物技术有限公司
Total bilirubin detection kit containing bacillus subtilis laccase
ActiveCN111424070AShort fermentation cycleReduce manufacturing costBacteriaMicrobiological testing/measurementBiotechnologySodium acetate
The invention discloses a total bilirubin detection kit containing bacillus subtilis laccase. The kit is composed of a reagent R1, a reagent R2, a reagent R3 and a reagent R4; the reagent R1 includesa buffer solution, a surfactant, mannitol, sodium nitrite, sodium azide, sodium ethylene diamine tetracetate, and the balance of water; the reagent R2 includes a buffer solution, KCl, polyethylene glycol-600, sodium cholate, and the balance of water; the reagent R3 is bacillus subtilis laccase; and the reagent R4 is a total bilirubin standard product. The bacillus subtilis laccase has a short fermentation cycle and low production costs, and can realize rapid mass production; the total bilirubin detection kit prepared by using the bacillus subtilis laccase can overcome the problems of poor stability and high costs of original bilirubin oxidase; and the kit provided by the invention has the advantages of good stability and low costs.
Owner:TIANJIN UNIV
Anti-interference and stable serum total bilirubin (enzymatic method) determination kit as well as preparation method and application thereof
ActiveCN112710854ANo reconstitution requiredImprove stabilityMicrobiological testing/measurementBiological testingActive agentSurface-active agents
The invention provides a total bilirubin (TB) determination kit. The kit contains a reagent R1 and a reagent R2, wherein the reagent R1 contains the following components: a trihydroxymethyl aminomethane hydrochloric acid buffer solution, NaCl, sodium cholate, citric acid, a surfactant and a preservative; and the reagent R2 comprises a trihydroxymethyl aminomethane hydrochloric acid buffer solution, bilirubin oxidase, a stabilizer and a preservative. The invention further provides a preparation method and application of the kit, the kit adopts different types of stabilizers and surfactants, interference of blood fat can be effectively avoided, and the kit is a liquid kit which is high in interference resistance, high in sensitivity, good in stability and low in cost.
Owner:中拓生物有限公司 +1
Conjugated bilirubin determination kit
ActiveCN113624701AInhibition of catalytic activityEliminate distractionsColor/spectral properties measurementsConjugated bilirubinActive agent
The invention discloses a conjugated bilirubin determination kit. The conjugated bilirubin determination kit comprises a reagent 1 and a reagent 2 which are independently packaged, wherein the reagent 1 is composed of a reagent 1a and a reagent 1b which are independently packaged; the reagent 1a is prepared from potassium hydrogen phthalate, sodium fluoride, disodium ethylene diamine tetraacetate, p-toluene sulfonic acid, N-acetylcysteine, laminarin, raffinose and a surfactant; the reagent 1b comprises potassium hydrogen phthalate, ascorbic acid oxidase, laminarin, raffinose, a surfactant and a liquid biological preservative; and the reagent 2 comprises potassium hydrogen phthalate, bilirubin oxidase, laminarin, raffinose, a surfactant and a liquid biological preservative. Compared with the prior art, the conjugated bilirubin determination kit provided by the invention has the advantages of high detection result accuracy, high precision, good stability, strong anti-interference capability and good linear relationship, and meets clinical requirements.
Owner:浙江夸克生物科技有限公司
Process for producing iodine from copper containing oxidases useful as iodine oxidases
The present invention relates to a method for oxidation of iodide which comprises contacting, in an aqueous solution, a copper-containing oxidaze enzyme and a source of ionic iodide(I-), for a time and under conditions sufficient to permit the conversion of ionic iodide to iodine by the enzyme. The copper-containing enzymes may be, for example, a laccase or a bilirubin oxidaze.
Owner:NOVO NORDISKBIOTECH INC
A stable and strong anti-interference ability serum phospholipid detection reagent and detection method
ActiveCN105543336BAvoid interferenceImprove anti-interference abilityMicrobiological testing/measurementGlycerolPolyethylene glycol
The invention relates to the technical field of serum phospholipid detection and particularly relates to a serum phospholipid detecting reagent. A reagent R1 comprises a buffer solution, phospholipase D, DAOS, ascorbic acid oxidase, bilirubin oxidase, polyethylene glycol 6000, cane sugar, xanthan gum, mannitol, trehalose, BSA, glycerol propoxylate-block-ethoxylate (Pluranic L64) and an aseptic. A reagent R2 comprises the buffer solution, 4-aminoantipyrine, peroxidase, choline oxidase, the polyethylene glycol 6000, the cane sugar, the xanthan gum, the mannitol, the trehalose, the BSA, the glycerol propoxylate-block-ethoxylate (Pluranic L64) and the aseptic. The HEPES buffer solutions and a novel Trinder reaction chromogen DAOS are adopted. A plurality of stabilizers are added to obviously improve stability of the detecting reagent. The bilirubin oxidase and the ascorbic acid oxidase are added, thus effectively avoiding interference caused by bilirubin and ascorbic acid and greatly improving interference-resisting capability of the detecting reagent. In addition, addition of the glycerol propoxylate-block-ethoxylate (Pluranic L64) which is an optional nonionic surfactant prevents reaction system turbidity, enhances substrate stability and improves the interference-resisting capability of the detecting reagent.
Owner:BIOBASE BIODUSTRY (SHANDONG) CO LTD
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