47 results about "Bilirubin oxidase" patented technology

A fuel cell is provided with an anode and a cathode. The anode is in electrical communication with an anode enzyme and the cathode is in electrical communication with a cathode enzyme. The anode enzyme is preferably an oxidase or a dehydrogenase. The cathode enzyme is a copper-containing enzyme, such as a laccase, an ascorbate oxidase, a ceruloplasmine, or a bilirubin oxidase. Preferably, the cathode enzyme is operable under physiological conditions. Redox polymers serve to wire the anode enzyme to the anode and the cathode enzyme to the cathode. The fuel cell can be very small in size because it does not require a membrane, seal, or case. The fuel cell can be used in connection with a biological system, such as a human, as it may operate at physiological conditions. By virtue of its size and operability at physiological conditions, the fuel cell is of particular interest for applications calling for a power source implanted in a human body, such as a variety of medical applications.

The invention relates to the technical field of serum 5'-ribotide hydrolase detection, in particular to a serum 5'-ribotide hydrolase detection reagent. A PIPES buffer solution is adopted, and various stabilizers are added to remarkably improve the stability of the reagent; beta-sodium glycerophosphate, bilirubin oxidase and ascorbic acid oxidase are added to effectively prevent interference caused by alkaline phosphatase, cholerythrin and ascorbic acid and greatly enhance the anti-interference capability of the reagent. Besides, a preferred novel amphoteric surfactant, dodecyl dimethyl betaine (BS-12), is added to prevent a reaction system from turbidity, enhance the stability of a substrate and improve the anti-interference capability of the reagent.

The invention relates to the field of serum direct bilirubin detection technologies, in particular to a serum direct bilirubin (oxidase method) detection reagent. A reagent R1 contains a buffer solution, sodium fluoride, NAC(N-acetylcysteine), sodium 4-methylbenzenesulfonate, lipase, ascorbic acid oxidase, alpha-cyclodextrin, sucrose, trehalose, PEG-6000, fatty alcohol polyoxyethylene ether (AEO-9) and a preservative; and a reagent R2 contains a buffer solution, bilirubin oxidase, sucrose, trehalose, PEG-6000, fatty alcohol polyoxyethylene ether (AEO-9) and a preservative. Through the reagent,the specificity and accuracy of direct bilirubin testing are obviously improved, turbidity of a reaction system can be prevented, a reaction is promoted, and the stability and interference resistanceof the reagent are further improved.

The present invention relates to a test paper strip for detecting the uric acid concentration in urine by using a color reaction. According to the present invention, an enzyme coloring agent, bilirubin oxidase and bamboo fibers are adhered on a test paper substrate, and the main components of the enzyme coloring agent of the test paper strip comprise uricase, horseradish peroxidase, and a coloring agent.

The invention discloses a high-efficiency enzymatic biological fuel battery cathode and a preparation method thereof. The biological fuel battery cathode comprises a substrate electrode, a carbon nano-material layer coated on the substrate electrode and an enzymatic layer coated on the carbon nano-material layer, wherein the enzymatic layer comprises the following a material a) and b): a) laccase or bilirubin oxidase, b) the laccase or the bilirubin oxidase, which is cross-linked by a cross linking agent. Compared with an ordinary enzymatic biological fuel battery cathode, the enzymatic biological fuel battery cathode of the invention has higher oxygen catalytic reduction efficiency.

The invention relates to the technical field of serum phospholipid detection and particularly relates to a serum phospholipid detecting reagent. A reagent R1 comprises a buffer solution, phospholipase D, DAOS, ascorbic acid oxidase, bilirubin oxidase, polyethylene glycol 6000, cane sugar, xanthan gum, mannitol, trehalose, BSA, glycerol propoxylate-block-ethoxylate (Pluranic L64) and an aseptic. A reagent R2 comprises the buffer solution, 4-aminoantipyrine, peroxidase, choline oxidase, the polyethylene glycol 6000, the cane sugar, the xanthan gum, the mannitol, the trehalose, the BSA, the glycerol propoxylate-block-ethoxylate (Pluranic L64) and the aseptic. The HEPES buffer solutions and a novel Trinder reaction chromogen DAOS are adopted. A plurality of stabilizers are added to obviously improve stability of the detecting reagent. The bilirubin oxidase and the ascorbic acid oxidase are added, thus effectively avoiding interference caused by bilirubin and ascorbic acid and greatly improving interference-resisting capability of the detecting reagent. In addition, addition of the glycerol propoxylate-block-ethoxylate (Pluranic L64) which is an optional nonionic surfactant prevents reaction system turbidity, enhances substrate stability and improves the interference-resisting capability of the detecting reagent.

The invention provides a total cholesterol detection reagent with high accuracy and disturbance resisting capacity and relates to the technical field of total cholesterol detection. A biological buffer CAPS (3-(hexanaphthene amino)-1-propanesulfonic acid) is used in the reagent R, and therefore the buffering ability is ensured; a surfactant octadecy trimethyl ammonium bromide and a surfactant EMULGEN-707 are added into the reagent R, the emulsification effect of the reagent is improved well, and the accuracy and specificity of the reagent are improved; stabilizers such as trehalose and mannitol are added into the reagent R, and the stability of the reagent can be improved effectively; hydroxy ethidene diphosphonic acid is added into the reagent R, heavy metal ions can be effectively chelated, and the accuracy of the reagent can be improved; chemical-resistant succinoxidase and bilrubin oxidase are added into the reagent, disturbance of ascorbic acid and bilirubin can be effectively removed, and the reagent is simple in preparation, low in price and very suitable for large-area clinical popularization.

The invention relates to the technical field of detection of serum adenosine deaminase and in particular relates to a serum adenosine deaminase detection reagent. R1 comprises buffer solution, 4-aminoantipyrine, PNP, XOD, POD, bilirubin oxidase, ascorbic acid oxidase, glycerin, polyethylene glycol 6000, ethylene glycol, mannitol, mycose, BSA, alkyl glycoside and preservatives; R2 comprises buffer solution, adenosine, 3-hydroxy-2,4,6-triiodobenzoic acid, glycerin, polyethylene glycol 6000, ethylene glycol, mannitol, mycose, BSA, alkyl glycoside and preservatives. With the adoption of the novel Trinder reaction chromogen substance 3-hydroxy-2,4,6-triiodobenzoic acid, multiple stabilizers are added, and the stability of the reagent is obviously improved; and due to the added bilirubin oxidase, ascorbic acid oxidase and novel nonionic surfactant alkyl glycoside (APG), interference of bilirubin and ascorbic acid is avoided, a turbid reaction system is prevented, and the substrate stability and the interference resistance of the reagent are enhanced.

The invention relates to the technical field of total bilirubin detection, especially to a serum total bilirubin (oxidase method) detection reagent. A reagent R1 contains a buffer solution, lipase, ascorbic acid oxidase, alpha-cyclodextrin, sucrose, trehalose, PEG-6000, castor oil polyoxyethylene ether (EL-20) and a preservative. A reagent R2 contains a buffer solution, bilirubin oxidase, sucrose, trehalose, PEG-6000, castor oil polyoxyethylene ether (EL-20) and a preservative. The interference of lipid turbidity and ascorbic acid oxidase can be effectively avoided and the anti-interference ability of the reagent can be greatly enhanced by optimizing a reaction system and adding the lipase, the alpha-cyclodextrin and the ascorbic acid oxidase. The adoption of the novel PIPES(piperazine-1,4-diethylsulfonic acid) buffer solution and the addition of stabilizers such as the sucrose, the trehalose, the polyethylene glycol 6000 can effectively improve the stability of the reagent. Moreover, the addition of the optimal novel non-ionic surfactant castor oil polyoxyethylene ether (EL-20) can prevent the reaction system from turbidity and further improve the stability and the anti-interference ability of the reagent.

The invention relates to the technical field of adenosine deaminase detection, in particular to an adenosine deaminase detection reagent. A reagent R1 comprises a buffer solution, 4-aminoantipyrine, BSA, cane sugar, trehalose, peroxidase, ascorbic acid oxidase, bilirubin oxidase and preservatives; a reagent R2 comprises a buffer solution, adenosine, EHSPT, BSA, cane sugar and preservatives. The adenosine deaminase detection reagent has the advantages that the phosphate buffer solution is adopted, the stabilizer BSA, the cane sugar and the trehalose are added, and accordingly, stability of the reagent is improved greatly; determination performance is improved remarkably, and stability and anti-interference capability of the reagent are enhanced.

The invention relates to the field of biological photovoltaic cells, and discloses a biological photovoltaic cell and a preparation method thereof. The biological photovoltaic cell provided by the invention contains a polymer formed by a structural unit as shown in a formula (1) and a capsule-like body; the number-average molecular weight of the polymer is 8 *10 < 3 >-2*10 < 4 >; the distributionindex of the polymer ranges from 1 to 2; and the cathode of the biological photovoltaic cell contains bilirubin oxidase. According to the biological photovoltaic cell, the anode containing the polymerand the capsule-like body and the cathode containing the bilirubin oxidase are matched with each other so as to be used, so that the energy density and photoelectric response performance of the biological photovoltaic cell can be greatly improved, and photoelectric conversion efficiency is improved.

The invention discloses a method for detecting glucose by using a paper-based electrochemical/colorimetric dual-mode sensor. A hydrophobic region and a hydrophilic region are prepared on paper by utilizing wax printing and laser cutting technologies, and a carbon electrode is printed by virtue of a silk-screen printing technology. Different areas of the paper chip are functionalized through different methods, visual pre-judgment of glucose can be achieved through the color development effect of 3, 3 ', 5, 5'-tetramethyl benzidine, and a simple, convenient and rapid method is provided. A biofuel cell is constructed by utilizing the specific catalysis of glucose oxidase to glucose and the reduction effect of bilirubin oxidase to oxygen, and the open-circuit voltage is recorded by virtue of an electrochemical workstation, so that the ultra-sensitive detection of glucose is realized.

The invention relates to the technical field of low density lipoprotein cholesterol detection, in particular to an accurate and efficient single agent low density lipoprotein cholesterol detecting reagent, wherein a reagent R comprises 50-100 mmol/L of buffer solution, 5g/L of magnesium chloride polyanion, 1-5 g/L of saccharide ring, 0.1-1 g/L of HDAOS (N-(2-hydroxy-3-sulfopropyl)-3,5-dimethyloxyaniline sodium salt), 0.1-1 g/L of surfactant 1, 0.1-1 g/L of surfactant 2, 5-10 g/L of surfactant 3, 1-5 KU/L of ascorbate oxidase, 1-3 KU/L of bilirubin oxidase, 1-3 KU/L of peroxidase, 0.1-0.3 g/L of 4-AA and 1-3 mL/L of preservative. The accurate and efficient single agent low density lipoprotein cholesterol detecting reagent has the advantages of being convenient, simple, accurate and efficient.

The invention discloses a solid fermentation method of bilirubin oxidase, which has the purposes of low cost, simple production process and convenient enzyme extraction. The solid fermentation method comprises the following steps of: (1) separating and screening myrothecium roridum from soil, and preparing a myrothecium roridum spore suspension; and (2) weighing 3-7 parts by weight of bran, placing the bran in a triangular flask, adding 13-17 parts by weight of distilled water, uniformly agitating the bran and the distilled water by using a glass tick, sterilizing under high pressure at 121-130 DEG C for 20-30 min, cooling, inoculating with 1-5 parts by weight of spore suspension prepared in the step (1), cultivating at 22-31 DEG C for 7-11 days, taking out the triangular flask, adding 50-70 parts by weight of distilled water into the triangular flask, leaching for 2-4 h through table concentrator oscillation, and filtering by using qualitative filter paper to obtain the bilirubin oxidase crude liquid. The invention has the advantages of abundant resources and easy acquisition of the raw materials, low production cost, simple, convenient and feasible enzyme extraction process and energy-saving and environmental-friendly production process.

The invention discloses an alpha-hydroxybutyrate dehydrogenase detection reagent with good stability and high accuracy, and relates to the field of alpha-hydroxybutyrate dehydrogenase detection technologies. Components of a reagent R1 of the alpha-hydroxybutyrate dehydrogenase detection reagent comprise buffer solution, BSA (bovine serum albumin), 2-ketobutyric acid, EMULGEN-707, etidronic acid, octadecy trimethyl ammonium bromide, alkylphenol ethoxylates (APEO), ascorbic acid oxidase, bilirubin oxidase, sucrose, trehalose, xanthan gum and preservatives; components of a reagent R2 of the alpha-hydroxybutyrate dehydrogenase detection reagent comprise buffer solution, NADH xanthan gum and preservatives. The alpha-hydroxybutyrate dehydrogenase detection reagent has the advantages that the etidronic acid is added into the reagent R1, so that heavy metal ions can be effectively chelated, and the accuracy of the alpha-hydroxybutyrate dehydrogenase detection reagent can be effectively improved; chemical acid resistant oxidase and the bilirubin oxidase are added into the alpha-hydroxybutyrate dehydrogenase detection reagent, so that interference of ascorbic acid and bilirubin can be removed; the stabilizer BSA, the sucrose, the trehalose and the xanthan gum are added into the alpha-hydroxybutyrate dehydrogenase detection reagent, so that the stability of the alpha-hydroxybutyrate dehydrogenase detection reagent can be improved; the measurement performance of the alpha-hydroxybutyrate dehydrogenase detection reagent can be obviously improved by the aid of the alkylphenol ethoxylates (APEO) which is a novel surfactant, and the stability of the alpha-hydroxybutyrate dehydrogenase detection reagent can be enhanced.

The invention discloses a direct bilirubin assay method and a kit. The direct bilirubin assay method utilizes an oxidase and a substrate thereof to react to form hydrogen peroxide, and then hydrogen peroxide and peroxidase react to release oxygen. Direct bilirubin in a solution is oxidized to biliverdin, and the absorption peak is decreased at 450 nm. The degree of decrease is recorded to calculate the direct bilirubin content. According to the invention, another oxidase which is cheap, easy to obtain and stable and can generate hydrogen peroxide and the substrate thereof are used to replace direct bilirubin oxidase; the cost is greatly reduced compared with the cost of an original direct bilirubin oxidase assay method; the experimental data acquired by the detection method proves that thesensitivity, accuracy and repeatability of the analysis are excellent; and the detection reagent has the advantages of high use safety, low environmental pollution and good practicability.

The invention provides a total bilirubin (TB) determination kit. The kit contains a reagent R1 and a reagent R2, wherein the reagent R1 contains the following components: a trihydroxymethyl aminomethane hydrochloric acid buffer solution, NaCl, sodium cholate, citric acid, a surfactant and a preservative; and the reagent R2 comprises a trihydroxymethyl aminomethane hydrochloric acid buffer solution, bilirubin oxidase, a stabilizer and a preservative. The invention further provides a preparation method and application of the kit, the kit adopts different types of stabilizers and surfactants, interference of blood fat can be effectively avoided, and the kit is a liquid kit which is high in interference resistance, high in sensitivity, good in stability and low in cost.

The invention discloses a conjugated bilirubin determination kit. The conjugated bilirubin determination kit comprises a reagent 1 and a reagent 2 which are independently packaged, wherein the reagent 1 is composed of a reagent 1a and a reagent 1b which are independently packaged; the reagent 1a is prepared from potassium hydrogen phthalate, sodium fluoride, disodium ethylene diamine tetraacetate, p-toluene sulfonic acid, N-acetylcysteine, laminarin, raffinose and a surfactant; the reagent 1b comprises potassium hydrogen phthalate, ascorbic acid oxidase, laminarin, raffinose, a surfactant and a liquid biological preservative; and the reagent 2 comprises potassium hydrogen phthalate, bilirubin oxidase, laminarin, raffinose, a surfactant and a liquid biological preservative. Compared with the prior art, the conjugated bilirubin determination kit provided by the invention has the advantages of high detection result accuracy, high precision, good stability, strong anti-interference capability and good linear relationship, and meets clinical requirements.

The present invention relates to a method for oxidation of iodide which comprises contacting, in an aqueous solution, a copper-containing oxidaze enzyme and a source of ionic iodide(I-), for a time and under conditions sufficient to permit the conversion of ionic iodide to iodine by the enzyme. The copper-containing enzymes may be, for example, a laccase or a bilirubin oxidaze.