Anti-interference and stable serum total bilirubin (enzymatic method) determination kit as well as preparation method and application thereof

A technology for total bilirubin and bilirubin oxidase, which is applied in the field of biochemical reagent determination, can solve the problems of long-term stability of reagents, easy to be affected by blood lipids, poor anti-interference ability, etc., and achieves enhanced anti-interference ability and excellent performance indicators. , the effect of excellent stability

Active Publication Date: 2021-04-27
中拓生物有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The diazonium salt method is widely used in clinical laboratories to detect bilirubin, but the method is susceptible to interference from many factors, and the reagents cannot be stable for a long time, which is affected to a certain extent in disease diagnosis
The vanadic acid oxidation method is easy to contaminate the instrument and affect o...

Method used

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  • Anti-interference and stable serum total bilirubin (enzymatic method) determination kit as well as preparation method and application thereof
  • Anti-interference and stable serum total bilirubin (enzymatic method) determination kit as well as preparation method and application thereof
  • Anti-interference and stable serum total bilirubin (enzymatic method) determination kit as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] A conventional total bilirubin assay kit includes reagent R1 and reagent R2.

[0051] Reagent R1 contains the following components:

[0052] Piperazine-1,4-diethanesulfonic acid (PIPES) buffer solution 50mmol / L;

[0053] NaCl 2g / L;

[0054] SDS 1g / L;

[0055] Sodium cholate 1g / L;

[0056] Citric acid 0.1g / L;

[0057] Sorbitan Laurate 1%;

[0058] Polyoxypropylene Mannitol Dioleate 1%;

[0059] proclin300 1 ml / L.

[0060] Reagent R1 has a pH value of 8.2.

[0061] Reagent R2 contains the following ingredients:

[0062] Piperazine-1,4-diethanesulfonic acid (PIPES) buffer solution 50mmol / L;

[0063] Bovine serum albumin (BSA) 5g / L;

[0064] Mannitol 10g / L;

[0065] Triton 100 1%;

[0066] Bilirubin oxidase 1KU / L;

[0067] proclin300 1ml / L.

[0068] Reagent R2 has a pH of 9.0.

[0069] Wherein said percentage is volume ratio.

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Abstract

The invention provides a total bilirubin (TB) determination kit. The kit contains a reagent R1 and a reagent R2, wherein the reagent R1 contains the following components: a trihydroxymethyl aminomethane hydrochloric acid buffer solution, NaCl, sodium cholate, citric acid, a surfactant and a preservative; and the reagent R2 comprises a trihydroxymethyl aminomethane hydrochloric acid buffer solution, bilirubin oxidase, a stabilizer and a preservative. The invention further provides a preparation method and application of the kit, the kit adopts different types of stabilizers and surfactants, interference of blood fat can be effectively avoided, and the kit is a liquid kit which is high in interference resistance, high in sensitivity, good in stability and low in cost.

Description

technical field [0001] The invention relates to the technical field of biochemical reagent determination, in particular to a total bilirubin determination kit, and also relates to a preparation method and application of the total bilirubin determination kit. Background technique [0002] Bilirubin is a type of bile pigment, the main pigment in human bile. Bilirubin is the main metabolite of iron porphyrin compounds in the body. It is toxic and can cause irreversible damage to the brain and nervous system, but it also has an antioxidant function and can inhibit the oxidation of linoleic acid and phospholipids. Bilirubin is an important basis for judging jaundice clinically, and it is also an important indicator of liver function. [0003] Total bilirubin: High indirect bilirubin and high direct bilirubin indicate hepatocellular jaundice, damage to liver cells, decreased liver function, and the liver cannot completely convert indirect bilirubin into direct bilirubin. The com...

Claims

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Application Information

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IPC IPC(8): G01N33/72C12Q1/26G01N33/52
CPCC12Q1/26G01N33/52G01N33/728
Inventor 刘安娜
Owner 中拓生物有限公司
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