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Accurate and efficient single agent low density lipoprotein cholesterol detecting reagent

A low-density lipoprotein and detection reagent technology, applied in the field of low-density lipoprotein cholesterol detection reagents, can solve the problems of occupying space and reducing convenience, and achieve the effect of removing interference and ensuring specificity

Inactive Publication Date: 2018-12-28
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The homogeneous method for detecting low-density lipoprotein cholesterol in blood has the advantages of simplicity, rapidity, good sensitivity, strong anti-interference ability, no need for sample pretreatment, and automatic analysis. However, the low-density lipoprotein cholesterol currently used in clinical applications Cholesterol detection reagents are composed of double reagents or multiple reagents, occupying more space in the equipment and reducing the convenience of use. In order to improve the convenience and practicability of the product, a single reagent low-density lipoprotein cholesterol detection reagent was invented. The advantages of convenience, speed, accuracy and efficiency

Method used

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  • Accurate and efficient single agent low density lipoprotein cholesterol detecting reagent
  • Accurate and efficient single agent low density lipoprotein cholesterol detecting reagent
  • Accurate and efficient single agent low density lipoprotein cholesterol detecting reagent

Examples

Experimental program
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Effect test

Embodiment 1

[0042] An accurate and efficient single reagent low-density lipoprotein cholesterol detection reagent, the composition of the reagent R is as follows:

[0043] MES ····················································· 50mmol / L,

[0044] magnesium chloride ·················································· 2.5g / L,

[0045] Dextran sulfate 50,000 (Shanghai Xibao Biological) ····························· 3g / L,

[0046] β-cyclodextrin ················································ 1g / L,

[0047] HDAOS (N-(2-Hydroxy-3-sulfopropyl)-3,5-dimethyloxyaniline sodium salt) ······· 0.1g / L,

[0048] AMPHITOL 20AB ··········································· 0.1ml / L,

[0049] Nonylphenol polyoxyethylene lauryl ether NP-07 ······························ 0.1g / L,

[0050] Polyoxyethylene polypropylene ether block copolymer (plUronic F-68) ··············· 5g / L,

[0051] ascorbate oxidase·········································· 1KU / L,

[0052] Bilirubin oxidase ·················...

Embodiment 2

[0059] 1) What this embodiment describes is an accurate and efficient single reagent low-density lipoprotein cholesterol detection reagent with increased content of important components. The composition of the reagent R is as follows:

[0060] MES ··························································· 100mmol / L,

[0061] magnesium chloride ························································ 5g / L,

[0062] Dextran sulfate 50,000 (Shanghai Xibao Biological) ··································· 5g / L,

[0063] β-cyclodextrin ······················································ 5g / L

[0064] HDAOS (N-(2-Hydroxy-3-sulfopropyl)-3,5-dimethyloxyaniline sodium salt) ············· 1g / L,

[0065] AMPHITOL 20AB ················································· 1g / L,

[0066] Nonylphenol polyoxyethylene lauryl ether NP-07 ···································· 1g / L

[0067] Polyoxyethylene polypropylene ether block copolymer (plUronic F-68) ····················· 10g / L ...

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Abstract

The invention relates to the technical field of low density lipoprotein cholesterol detection, in particular to an accurate and efficient single agent low density lipoprotein cholesterol detecting reagent, wherein a reagent R comprises 50-100 mmol / L of buffer solution, 5g / L of magnesium chloride polyanion, 1-5 g / L of saccharide ring, 0.1-1 g / L of HDAOS (N-(2-hydroxy-3-sulfopropyl)-3,5-dimethyloxyaniline sodium salt), 0.1-1 g / L of surfactant 1, 0.1-1 g / L of surfactant 2, 5-10 g / L of surfactant 3, 1-5 KU / L of ascorbate oxidase, 1-3 KU / L of bilirubin oxidase, 1-3 KU / L of peroxidase, 0.1-0.3 g / L of 4-AA and 1-3 mL / L of preservative. The accurate and efficient single agent low density lipoprotein cholesterol detecting reagent has the advantages of being convenient, simple, accurate and efficient.

Description

technical field [0001] The invention relates to the technical field of low-density lipoprotein cholesterol detection, in particular to an accurate and efficient single-reagent low-density lipoprotein cholesterol detection reagent. Background technique [0002] Cholesterol exists in the blood as lipoproteins. Low-density lipoprotein (LDL) in plasma is the main carrier for transporting endogenous cholesterol, and LDL is degraded and transformed by binding to the low-density lipoprotein receptor (LDL-R) on its cell membrane. [0003] The concentration of low-density lipoprotein cholesterol (LDL-C) has a significant positive correlation with the incidence of coronary heart disease, and LDL-C has become an important indicator for evaluating the risk factors of individual coronary heart disease. The occurrence of sclerosis and coronary heart disease was positively correlated. The National Cholesterol Education Program (NCEP) of the United States regards LDL-C as the primary solu...

Claims

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Application Information

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IPC IPC(8): C12Q1/60C12Q1/26C12Q1/28C12Q1/44
CPCC12Q1/26C12Q1/28C12Q1/44C12Q1/60
Inventor 罗维晓胡晓飞包兴艳王美丽
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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