Creatininase method detection kit

A detection kit and creatinase technology, applied in the field of medical testing, can solve problems such as environmental pollution and high toxicity, and achieve the effects of strong anti-interference ability, high accuracy and improved stability

Inactive Publication Date: 2019-03-01
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Antibacterial preservatives currently used in diagnostic kits are mainly chemical preservatives and antibiotic preservatives, but chemical preservatives have problems such as high toxicity and environmental pollution. Antibiotic preservatives are likely to cause bacterial resistance and will Lead to the production of super bacteria, which has great risks to human health

Method used

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  • Creatininase method detection kit
  • Creatininase method detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The creatinine enzymatic detection kit of this embodiment is composed of reagent R1 and reagent R2, wherein:

[0046] The composition of reagent R1 is: Tris buffer: 20mmol / L, pH7.50; creatinase: 20KU / L; sarcosine oxidase: 15.2KU / L; ascorbate oxidase: 18KU / L; bilirubin oxidase: 80KU / L; TOOS 2mmol / L; peroxidase 40KU / L; BSA 20g / L; trehalose 10g / L; Tween20 0.3g / L; TgHb 1-5 0.5g / L.

[0047] The composition of reagent R2 is: Tris buffer: 30mmol / L, pH7.80; 4-aminoantipyrine 3mmol / L; creatinase 400KU / L; BSA 5g / L; Tween80 0.3g / L; TgHb 1-5 0.4 g / L.

Embodiment 2

[0049] The creatinine enzymatic detection kit of this embodiment is composed of reagent R1 and reagent R2, wherein:

[0050] The composition of reagent R1 is: HEPES buffer: 50mmol / L, pH8.0; creatinase: 40KU / L; sarcosine oxidase: 29KU / L; ascorbate oxidase: 50KU / L; bilirubin oxidase: 40KU / L; TOOS 5mmol / L; peroxidase 60KU / L; sucrose 10g / L; TritonX-100 0.2g / L; Aurelin 0.2g / L.

[0051] The composition of reagent R2 is: PIPES buffer: 50mmol / L, pH8.2; 4-aminoantipyrine 5mmol / L; creatinase 600KU / L; fructose 15g / L; TritonX-100 0.5g / L; Aurelin 0.8g / L.

Embodiment 3

[0053] The creatinine enzymatic detection kit of this embodiment is composed of reagent R1 and reagent R2, wherein:

[0054] The composition of reagent R1 is: MOPS buffer: 30mmol / L, pH8.5; creatinase: 62KU / L; sarcosine oxidase: 35KU / L; ascorbate oxidase: 80KU / L; bilirubin oxidase: 15KU / L; TOOS 6mmol / L; peroxidase 45KU / L; glycerol 20g / L; SPAN20 0.1g / L; MCdef: 0.6g / L.

[0055] Reagent R2 consists of: MPOS buffer: 50mmol / L, pH8.4; 4-aminoantipyrine 6mmol / L; creatinase: 700KU / L; mannitol: 10g / L; glycerol: 10g / l; SPAN20 0.21 g / L; MCdef: 0.4g / L.

[0056] In the present invention, TgHb 1-5 is clam antimicrobial peptide (shellfish), prepared according to Chinese patent application CN2017104041998; Aurelin is jellyfish antimicrobial peptide, prepared according to Chinese patent application CN2017104041983; MCdef is clam antimicrobial peptide, obtained according to Chinese patent application CN2017104041983 Patent application CN2017104041964 is prepared; or in the present invention, ...

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Abstract

The invention discloses a creatininase method detection kit. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 is prepared from a buffer solution, creatinase, sarcosine oxidase,ascorbic acid oxidase, bilirubin oxidase, TOOS, peroxidase, a stabilizer, and a surfactant; the reagent R2 is prepared from a buffer solution, 4-aminoantipyrine, creatininase, a stabilizer, a surfactant, and 0.1 to 10g / L anti-bacterial preservative. The creatininase method detection kit has the advantages of lowering the toxicity effectively, lowering the environment pollution, preventing the drugtolerance of bacteria, preventing super bacteria from generating and lowering the human health risk, along with high stability, and can be applied to creatinine detection of chronic disease management.

Description

Technical field: [0001] The invention relates to the technical field of medical testing, in particular to a creatinine detection reagent for chronic disease management, more specifically to a stable creatinine enzyme detection kit. Background technique: [0002] Creatinine (CR) is a product of human muscle metabolism. It is mainly formed slowly by creatine through an irreversible non-enzymatic dehydration reaction, and then released into the blood and excreted with urine. It is basically not affected by diet and exercise, and can be filtered through the glomerulus. However, it is rarely reabsorbed in the renal tubules. Creatinine includes blood creatinine and urine creatinine. Serum creatinine is more meaningful to measure renal function. It is of great significance to detect the degree of substantial damage to the function in clinical practice to help judge kidney and other diseases. [0003] According to reports, the epidemiological survey of urban and rural residents in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/70G01N33/543G01N33/52
CPCG01N33/52G01N33/54393G01N33/70G01N2800/347
Inventor 邹炳德邹继华章玉胜贾江花汪屹武强
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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