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78 results about "Sarcosine oxidase" patented technology
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Sarcosine oxidase is an enzyme (EC 1.5.3.1) that catalyzes the oxidative demethylation of sarcosine to yield glycine, H₂O₂, 5,10-CH₂-tetrahydrofolate in a reaction requiring H₄-tetrahydrofolate and oxygen. Corynebacterial sarcosine oxidase is a heterotetramer and is produced as an inducible enzyme when Corynebacterium sp.is grown with sarcosine as source of carbon and energy.
Stable three enzyme creatinine biosensor
InactiveUS20090045056A1Controlled diffusionAvoid contactImmobilised enzymesBioreactor/fermenter combinationsCreatinine risePolyurethane membrane
The invention provides methods for preparing a stable, multiple-use three enzyme biosensor for the amperometric determination of creatinine in biological liquids that has a useful lifetime that extends significantly beyond that of presently available amperometric biosensors. The biosensor prepared by the methods of the invention encompasses a plurality of immobilized enzymes that are applied to the biosensor as an enzyme-polymer composition. The enzymes, which can include creatinine amidohydrolase, creatine amidinohydrolase and sarcosine oxidase, are immobilized into the enzyme-polymer composition simultaneously as well as applied to the biosensor simultaneously. Prior to being immobilized, the enzymes can be chemically modified by attaching one or more polyethylene glycol (PEG) chains per enzyme monomer. The polymer component can be provided by a polyurethane membrane. The invention also provides a method of preparing a biosensor that limits the diffusion of silver ions emanating the reference electrode, thereby preventing, contact between the silver ions and the enzymes. Related methods of preparing an enzyme-polymer composition for incorporation into a multiple use three enzyme biosensor for the amperometric determination of creatinine in biological liquids also are provided. The invention also provides multiple-use biosensors and enzyme-polymer compositions prepared by the methods disclosed.
Owner:BERBERICH JASON +3
Enzyme combining stabilizer
ActiveCN1986785APlay a role in quality stabilityPeptide/protein ingredientsEnzyme stabilisationLactate dehydrogenaseCreatinine rise
The present invention provides a kind of efficient enzyme combining stabilizer with high stabilizing effect on enzymes for clinical diagnosis reagent. The enzyme combining stabilizer consists of bovine serum albumin, EGTA, 1, 2-dithio threitol, potassium gluconate, sodium chloride, proclin300, magnesium acetate, etc. It has powerful stabilizing effect on lactate dehydrogenase, sarcosie oxidase, urease, creatinine enzyme, creatine hydrolase, etc.
Owner:SHANGHAI FOSUN LONG MARCH MEDICAL SCI CO LTD +1
Kit and method for assaying creatinine
ActiveCN106198509AMaterial analysis by observing effect on chemical indicatorMicrobiological testing/measurementSarcosine oxidaseCreatininase
The invention relates to a kit and a method for assaying creatinine. The kit includes a first reagent group and a second reagent group. The first reagent group includes creatinase, sarcosine oxidase, chromogen and catalase; the second reagent group includes creatininase, 4-amino antipyrine and peroxidase. The chromogen is selected from N-(2-hydroxyl-3-sulfopropyl)-3,5-dimethoxyaniline or a sodium salt thereof, and N-ethyl-N-(2-hydroxyl-3-sulfopropyl)-3,5-dimethoxyaniline. The kit and the method can reduce interference during the detection of the creatinine due to the calcium dobesilate and / or etamsylate that exist in a sample.
Owner:PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI +1
Creatinine detection reagent
ActiveCN103278468AHigh sensitivityImprove accuracyColor/spectral properties measurementsMethylanilineCreatininase
The invention discloses a creatinine detection reagent which is characterized by comprising a diluent and a reaction reagent, wherein the diluent is composed of buffer, surfactant, preservative, anti-bilirubin interference agent and vitamin C oxidase; and the reaction reagent is composed of buffer, creatinase, sarcosine oxidase, peroxidase, N-ethyl-N-(3-propylsulfo)-3-methylaniline, creatininase, 4-aminoantipyrine, preservative and freeze-drying protective agent. The detection reagent disclosed by the invention has favorable sensitivity, accuracy, precision and linearity, and can completely satisfy the clinical examination requirements.
Owner:NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
Detection kit for determining content of creatinine in serum by enzymic method
InactiveCN104198408AImprove stabilityEliminate distractionsMaterial analysis by observing effect on chemical indicatorColor/spectral properties measurementsCreatinine riseCreatininase
The invention discloses a detection kit for determining the content of creatinine in serum by an enzymic method. The detection kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 contains 3-10g / L of buffering solution with the pH value being 7.5-8.0, 0.2-0.5g / L of EDTA, 0.5-2g / L of N-ethyl-N-(2-hydroxyl-3-sulfopropyl) m-toluidine sodium salt, 1 per mill-5 per mill of a surface active agent, 0.2-1g / L of sarcosine oxidase, 1-5KU / L of ascorbate oxidase, 2-5KU / L of creatinase and 0.5-1g / L of a stabilizing agent; the reagent R2 contains 3-10g / L of buffering solution with the pH value being 7.5-8.0, 0.1-0.5g / L of 4-aminoantipyrine, 0.1-0.4g / L of potassium ferricyanide, 2-8g / L of creatininase amidohydrolase, 1-6KU / L of peroxidase and 0.5-2g / L of a preservative. The detection kit disclosed by the invention is excellent in stability, strong in interference resistance and high in clinical application value.
Owner:上海睿康生物科技有限公司
Dry test strip and method for measuring creatinine
ActiveCN102757893ABioreactor/fermenter combinationsBiological substance pretreatmentsSarcosine oxidaseCreatinine hydrolase
A dry test strip for measuring creatinine (1) comprises: a support (2),a reagent layer (4) that is disposed on the support (2),a reagent holding layer (5) that is disposed on the reagent layer (4),and a connection layer (6) that is composed of an adhesive which adhesively bonds the reagent layer (4) to the reagent holding layer (5) and is formed in spot form, wherein the reagent layer (4) contains creatininase and 4-aminoantipyrine,the reagent holding layer (5) contains creatinase, sarcosine oxidase, peroxidase, and N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline,and the connection layer (6) delays arrival of a liquid sample spot-deposited on the reagent holding layer (5) at the reagent layer (4).
Owner:ARKRAY INC
Sarcosine detection device as well as preparation method and application thereof
ActiveCN106770588ASimple structureReduce manufacturing costMaterial analysis by electric/magnetic meansSarcosine oxidasePotential change
The invention discloses a sarcosine detection device as well as a preparation method and application thereof. The sarcosine detection device comprises an organic electrochemical transistor and a gate electrode with sarcosine oxidase immobilized. When the sarcosine to be detected has chemical reaction with the sarcosine oxidase on the gate electrode, the interfacial potential change of the gate electrode is caused, and finally, the detection of the sarcosine concentration is achieved by measuring the change of the channel current of the organic electrochemical transistor. The organic electrochemical transistor adopted by the invention has the functions of sensing and signal amplification, and has very high sensitivity and very low detection limit in sarcosine detection; and the sarcosine detection device provided by the invention has the advantages of simple structure, low production cost and low working voltage, and can achieve portable detection.
Owner:SHENZHEN UNIV
Composition for eliminating interference of calcium dobesilate medicine on creatininase-method detection
ActiveCN108627654ASolve the problem of serious negative interferenceDisease diagnosisBiological testingHigh concentrationCreatininase
The invention relates to a composition for eliminating the interference of a calcium dobesilate medicine on creatininase-method detection. The composition comprises an R1 reagent, an R2 reagent and ahigh molecular oxidant, wherein the high molecular oxidant is one or more of high molecular peroxy acid, high molecular selenium oxide, high molecular sulfur chloride ether, N-substituted imide, water-soluble tetrazole and a Dess-Martin oxidant. The high molecular oxidant in the composition disclosed by the invention can react with the calcium dobesilate in a sample to be detected, the calcium dobesilate loses the effect of interfering enzyme-method creatinine detection and the accuracy of sarcosine-oxidase-method detection is improved, so that the problem of serious negative interference, caused by high-concentration calcium dobesilate in blood of a patient, on an enzyme-method creatinine project detection result when the patient orally takes the calcium dobesilate medicine is solved.
Owner:WUHAN HANHAI NEW ENZYMES BIOLOGICAL TECH CO LTD
A dry-sheet type serum creatinine detection reagent strip and a preparing method thereof
A dry-sheet type serum creatinine detection reagent strip and a preparing method thereof are disclosed and belong to the field of in-vitro diagnosis. A creatinine reacting membrane, a filtering membrane and a diffusion layer in the reagent strip are fixed to a plastic plate in an appropriately overlapping manner. The reagent strip is characterized in that the reacting membrane includes a diluting liquid and a reacting reagent, the diluting liquid includes a buffer liquid, a surfactant, a stabilizer, an adhesive, a coenzyme factor and an aseptic, and the reacting reagent includes creatinine amidohydrolase, creatinase, sarcosine oxidase, peroxidase, 4-aminoantipyrine and N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethylaniline. The Trinder reagent is utilized as an indicator probe, an analyte finally generates a colored quinone compound through various enzyme functions, and the creatinine content of a sample is indirectly detected through the coloration degree of the quinone compound. According to the reagent strip and the method, a liquid phase reaction is transferred to a solid carrier, objectives of simple operation and rapid and convenient detection of the creatinine content of the sample are achieved, and the reagent strip and the method are high in sensitivity, good in accuracy and convenient in popularization and application.
Owner:GETEIN BIOTECH
Modified sarcosine oxidases, genes and recombinant DNAs thereof, and methods for preparing the same
ActiveUS20050026265A1High activityImprove stabilityFungiSugar derivativesSarcosine oxidaseProtein composition
(1) A modified sarcosine oxidase with improved stability in the acidic range compared to a wild-type sarcosine oxidase. (2) A sarcosine oxidase gene encoding a modified sarcosine oxidase of the following (a), (b), or (c): (a) protein composed of the amino acid sequence represented by SEQ ID NO: 1 (b) protein composed of an amino acid sequence wherein one or some amino acid(s) are deleted, substituted, or added from the amino acid sequence represented by SEQ ID NO: 1, and which has sarcosine oxidase activity (c) protein composed of an amino acid sequence which shows 80% or more homology to the amino acid sequence represented by SEQ ID NO: 1, and which has sarcosine oxidase activity According to the present invention, sarcosine oxidases, in particular sarcosine oxidases which show optimal pH and high activity in the slightly acidic range and have improved stability can be prepared efficiently, thus making the invention industrially useful.
Owner:KIKKOMAN CORP
Determination of creatinine content and creatinine diagnostic reagent kit
InactiveCN1778944AFree from pollutionThe test result is accurateMicrobiological testing/measurementCreatinine riseAbsorbance
The invention is about a method of measuring the content of creatinine, and it also concerns the reagent box of creatinine diagnosis. This invention belongs to the field of medical testing and measuring technology. The reagent box is consisted of buffer solution, creatinine enzyme, creatine enzyme, sarcosine onidase, anaerooxidase and stabilizer. Firstly, we cause an enzyme-coupled reaction through mixing the sample and the reagent according to a certain proportion of volume; secondly, put the final reactant under the biochemical analyzer and test the absorbance variational situation (speed) of dominant wavelength; then we can get the content of creatinine. By using this invention, we can get the necessary measuring result with high sensitiveness and fine precision through biochemical analyzer, and the result would not be contaminated by material of internal and exogenous sources. Thus, this method can be conveniently promoted and applied.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Creatinine measurement reagent
InactiveCN103773833AAvoid interferenceImprove stabilityMicrobiological testing/measurementSarcosine oxidaseCreatinine Measurement
The invention discloses a creatinine measurement reagent which comprises a reagent I and a reagent II, wherein the reagent I comprises 10-200mmol / L Good's buffering solution with the PH of 7.0-9.0, 5-120KU / L creatine amidine hydrolase, 2-50KU / L sarcosine oxidase, 1-10ml / L surfactant, 1-100mmol / L stabilizing agent and 0.1-50ml / l preservative; the reagent II comprises 10-200mmol / L Good's buffering solution with the PH of 7.0-9.0, 0.1-5mml / L NAD<+>, 3-200KU / L creatinine amidohydrolase, 1-50KU / L formaldehyde dehydrogenase, 1-10ml / L surfactant, 1-100mmol / L stabilizing agent and 0.1-50ml / l preservative. According to the creatinine measurement reagent disclosed by the invention, the problem of interference is solved; and the reagent components are stable.
Owner:深圳市雷诺华科技实业有限公司
Detection reagent and test strip for creatinine
The invention discloses a detection reagent and a test strip for creatinine. Each liter of the detection reagent contains 12-18 KU of creatininase, 12-20 KU of creatinase, 9-12 KU of sarcosine oxidase, 15-20 KU of horseradish peroxidase, 5-8 KU of ascorbic acid oxidase, 0.20-0.35 g of 4-aminoantipyrine and 0.20-0.30 g of color developing matter. The detection reagent contains various specific enzymes for specific proportioning, and can achieve the purposes of rapid detection and more accurate detection. The test strip comprises a reaction base layer, a reaction layer, a blood filter layer and a hydrophilic layer which are stacked to form a siphon system, thereby further guaranteeing rapid detection and more accurate detection.
Owner:复星诊断科技(长沙)有限公司
Biosensor structures for improved point of care testing and methods of manufacture thereof
ActiveUS9487811B2Microbiological testing/measurementVacuum evaporation coatingPoint of careSarcosine oxidase
Owner:ABBOTT POINT CARE
Test strip for detecting creatinine by electrochemical method and preparation method thereof
PendingCN111189899ARapid quantitationQuantitatively accurateMaterial analysis by electric/magnetic meansDisease diagnosisCreatininasePeroxidase
The invention discloses a test strip for detecting creatinine by an electrochemical method. The test strip comprises: a substrate, a working electrode, a reference electrode, a counter electrode, a startup electrode, an insulating layer, a first enzyme carrier layer, a second enzyme carrier layer, a blood filtering membrane, a diffusion layer, a double faced adhesive tape layer, siphon holes and ahydrophilic layer. A formula of a first reaction solution on the first enzyme carrier layer comprises: creatininase, sarcosine oxidase, creatine hydrolase, ascorbic acid oxidase, peroxidase, a dispersing agent, a surfactant and a buffer solution; a formula of a second reaction solution on the second enzyme carrier layer comprises: sarcosine oxidase, creatine hydrolase, ascorbic acid oxidase, peroxidase, a stabilizer, a surfactant and a buffer solution; the detection result of the invention is well matched with a hospital inspection result, and the use is simple.
Owner:杭州联晟生物科技有限公司
Modified sarcosine oxidase, process for producing the same and reagent composition using the same
The present invention discloses a protein, which still has sarcosine oxidase activity after being modified by adding, deleting, inserting or replacing at least one amino acid in the amino acid sequence constituting the protein having sarcosine ammoniaase activity, characterized by Improved stability in liquid compared to unmodified protein and / or reduced effect on L-proline compared to unmodified protein. Sarcosine oxidase has at least one of the following characteristics, namely, based on the effect of creatine on L-proline, measured at 37°C and pH 8.0, is 0.7% or less and the Km of L-proline The value is 150 mM or more; the method for producing sarcosine oxidase having excellent substrate specificity comprises culturing a microorganism capable of producing sarcosine oxidase and collecting sarcosine oxidase from the culture medium; and containing sarcosine A reagent for the detection of creatinine for amino acid oxidase.
Owner:TOYO TOYOBO CO LTD
Preparation method and application of sarcosine oxidase
InactiveCN103013941AEasy to operateReduce manufacturing costMicrobiological testing/measurementEnzymesSarcosine oxidaseThiogalactosides
The invention discloses a preparation method of sarcosine oxidase. The method comprises the following steps: transforming a whole-gene synthesis humanization sarcosine oxidase coding deoxyribonucleic acid sequence, and removing escherichia coli rare codon; inserting the transformed sarcosine oxidase coding deoxyribonucleic acid sequence into an expression carrier, and carrying out transformation and expression by using an escherichia coli strain; inoculating the strain subjected to transformation and expression into a luria-bertani (LB) culture medium, and carrying out isopropyl-beta-d-thiogalactoside (IPTG) induction; and breaking the obtained thallus, centrifugally separating and purifying through an Ni-nitrilotriacetic acid (NTA) column. The method is simple in sarcosine oxidase preparation operation, low in preparation cost, high in finished product yield, high in purity and high in biological activity, and can be used as a diagnostic reagent for sarcosine assay kit research.
Owner:BEIJING HOSPITAL
Biosensor structures for improved point of care testing and methods of manufacture thereof
ActiveUS20140262777A1Immobilised enzymesBioreactor/fermenter combinationsPoint of careSarcosine oxidase
The present invention relates to analytical testing devices and methods for fabricating electrochemical creatinine biosensors, and in particular using point of care electrochemical biosensors for testing for creatinine in samples. For example, the present invention may be directed to a biosensor having an electrode, a first printed layer formed on the electrode and having a first matrix that includes creatinine amidohydrolase (CNH), creatine amidinohydrolase (CRH), and sarcosine oxidase (SOX), and second printed layer formed over the first printed layer and having a second matrix that includes CRH, SOX, and catalase.
Owner:ABBOTT POINT CARE
Sarcosine oxidase affinity medium and method for synthesizing and purifying sarcosine oxidase
ActiveCN103055825AEfficient purificationImprove purification efficiencyOther chemical processesSolid sorbent liquid separationSarcosine oxidaseSubstrate analog
The invention discloses a sarcosine oxidase affinity medium and a method for synthesizing and purifying sarcosine oxidase, which belong to the technical field of biologic engineering. The sarcosine oxidase affinity medium disclosed by the invention is prepared by connecting a sarcosine oxidase affinity ligand with a chromatography medium; preferably, the sarcosine oxidase affinity ligand is a substrate analog 4-amino pyrrole-2-carboxylic acid, and the chromatography medium is Sepharose or poly-chitosan; the invention further provides a synthesis method of the sarcosine oxidase affinity medium, and a method for purifying the sarcosine oxidase in a large scale by using the sarcosine oxidase affinity medium. By utilizing the sarcosine oxidase affinity medium disclosed by the invention, the sarcosine oxidase can be rapidly separated and purified in a large scale, and a sarcosine oxidase pure product can be obtained under the condition of one step affinity chromatography; and the whole purification step is short in time, the protein and activity recovering rate is high, and thus being applicable to large-scale popularization and application.
Owner:JIANGNAN UNIV
Dry chemical reagent tablet for quantitatively measuring concentration of creatinine and preparation method of dry chemical reagent tablet
InactiveCN109916895ANo preparation requiredEasy to operateMaterial analysis by observing effect on chemical indicatorColor/spectral properties measurementsHemolysisCreatinine rise
Owner:吉林省汇酉生物技术股份有限公司
Stable creatinine detection kit and use method thereof
The invention discloses a stable creatinine detection kit and a use method thereof, relates to the field of biochemical detection and aims to provide a creatinine detection kit which is low in toxicity, high in stability and sensitive and accurate in detection and a use method of the creatinine detection kit. The creatinine detection kit comprises a reagent 1 and a reagent 2, wherein the reagent 1 is prepared from an HEPES buffer, EDTA (ethylenediaminetetraacetic acid), creatinase, sarcosine oxidase, erythromycin, PEG8000, tween 20, TOOS, a compound stabilizer and a proclin preservative; the reagent 2 is prepared from the HEPES buffer, catalase, creatininase, 4-aminoantipyrine, NPEO, potassium ferricyanide, sodium chloride, ethylene glycol and other substances. The kit has good compatibility, better stability and low toxicity and can resist interference of bilirubin and metal ions.
Owner:WHITMAN BIOTECH NANJING
Creatinine assay kit and application thereof
ActiveCN109613226ASimple componentsImprove stabilityAnalysis by subjecting material to chemical reactionColor/spectral properties measurementsSarcosine oxidaseCreatinine rise
The invention relates to a creatinine assay kit and application thereof, and belongs to the technical field of biological detection. The creatinine assay kit is composed of a reagent R1 and a reagentR2; the reagent R1 is prepared from creatinase, catalase, sarcosine oxidase, 4-amino-antipyrine, an inhibitor and a buffer solution; and the reagent R2 is prepared from creatinase, catalase, a color developing agent, ascorbic acid oxidase, an inhibitor and a buffer solution. According to the creatinine assay kit, assaying is conducted through a sarcosine oxidase method, and the creatinine assay kit has the advantages of wide linear range, good stability and the like.
Owner:浙江夸克生物科技有限公司
Creatininase method detection kit
InactiveCN109406798AHigh sensitivityImprove accuracyDisease diagnosisBiological testingBiotechnologyCreatininase
The invention discloses a creatininase method detection kit. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 is prepared from a buffer solution, creatinase, sarcosine oxidase,ascorbic acid oxidase, bilirubin oxidase, TOOS, peroxidase, a stabilizer, and a surfactant; the reagent R2 is prepared from a buffer solution, 4-aminoantipyrine, creatininase, a stabilizer, a surfactant, and 0.1 to 10g / L anti-bacterial preservative. The creatininase method detection kit has the advantages of lowering the toxicity effectively, lowering the environment pollution, preventing the drugtolerance of bacteria, preventing super bacteria from generating and lowering the human health risk, along with high stability, and can be applied to creatinine detection of chronic disease management.
Owner:NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
Sarcosine detection method based on metal organic framework material
ActiveCN110702670AIncrease the areaLarge porous poresMaterial analysis by observing effect on chemical indicatorChemiluminescene/bioluminescenceSarcosine oxidaseMetal-organic framework
The invention relates to a sarcosine detection method based on a metal organic framework material. The sarcosine detection method is characterized in that sarcosine oxidase and catalase are wrapped bymeans of the metal organic framework material, so that the pair of cascade enzymes is limited in a smaller space, and the content of sarcosine is determined by adopting a chemiluminescence method after a cascade reaction. The sarcosine detection method adopts ZIF-8 to coat SOX and HRP; ZIF-8 has a large specific surface area and porous channels, so that a reaction substrate in a solution can penetrate through the channels to react with SOX and HRP, enrichment of the substrate around the enzymes is facilitated, and the local substrate concentration is increased; meanwhile, due to wrapping of ZIF-8, SOX and HRP are limited within the range of hundreds of nanometers, the distance between SOX and HRP is regulated and controlled, hydrogen peroxide generated by catalyzing sarcosine by means ofSOX can be effectively transmitted to HRP for a chromogenic reaction, and the reaction rate of the reaction cascade enzymes is increased.
Owner:SHANGHAI NAT ENG RES CENT FORNANOTECH
Creatinine content detecting reagent and kit, and manufacturing and using methods of kit
ActiveCN102375066AHigh sensitivityImprove anti-interference abilityBiological testingFluorescence/phosphorescenceCreatinine riseCreatininase
The invention relates to a creatinine content detecting reagent and kit and manufacturing and using methods of the kit. The creatinine content detecting reagent comprises creatininase, creatinase, creatine oxidase and horseradish peroxidase; and a fluorescent dye, i.e., 5(6)-carboxyl-2,7-dichlordihydro fluorescein ester dipivalate comprises a dye bottom layer, a protease fixing layer and a top layer protecting layer. The using method has high selectivity; a protease has high specificity to reactants; the creatininase can be only used for catalyzing the hydrolysis reaction of creatinine, and does not have catalysis activity on other reaction substrates; three enzymes with high specificity are related to the detection process, so that the selectivity of the method is increased exponentially; the response fed by the kit is fluorescent response; and compared with certain methods for detecting color variations, the invention has the advantages of high sensitivity and interference resistance under the action of the inherent characteristics of fluorescent response.
Owner:TIANJIN HEOWNS BIOCHEM TECH
Creatinine detection kit and use method thereof
PendingCN112029817AHigh accuracy of test resultsImprove accuracyMicrobiological testing/measurementBiological material analysisHigh creatinineCreatininase
The invention provides a creatinine detection kit and a use method thereof. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises creatinase, sarcosine oxidase, peroxidase,ascorbic acid oxidase, 2, 4, 6-tribromo-3-hydroxybenzoic acid and 3, 5-dichloro-2-hydroxybenzenesulfonic acid; and the reagent R2 comprises creatinase, peroxidase, potassium ferrocyanide and 4-aminoantipyrine. The creatinine detection kit provided by the invention can resist interference of etamsylate with the concentration of 300mg / L (the highest blood concentration) or below in a to-be-detectedsample, has a wider linear range (0-6000 millimoles / L), is applicable to detection of samples with high creatinine content and can correctly evaluate renal functions of surgical patients.
Owner:LANZHOU BAIYUAN GENE TECH
Method for quantitative determination content of sarcosine and reaction kits
ActiveCN101587076AQuick checkEasy to detectFluorescence/phosphorescenceSarcosine oxidaseFluorescence spectra
The invention provides a method for quantitative determination content of sarcosine and reaction kits, the method includes steps: S1, preparing sarcosine solution of different concentration, adding reaction agent to the solution to react sarcosine with peroxide completely; S2, detecting fluorescence spectra of products fluorescent dimer in S1 respectively, drawing relationship curve of sarcosine content-flurescence; S3, using the method of S1 to make sarcosine samples react as described in S1, measuring fluorescence emission spectra of reaction product to obtain fluorescence data, then comparing with the relationship curve of sarcosine content-flurescence to obtain content of unknown sarcosine samples. Components of the reaction kits according to the invention includes methyl phenol or P-hydroxy acid solution, sarcosine oxidase, peroxydase and alkaline buffer. Method according to the invention is very suitable for large-scale analytica test of minim sarcosine in urine sample or blood sample.
Owner:泰安泰笙医药科技有限公司
Creatinine kit for eliminating calcium dobesilate and etamsylate and preparation method thereof
ActiveCN111733208AImprove stabilityEasy to operateMicrobiological testing/measurementBiological material analysisActive agentPeroxidase
The invention relates to a creatinine kit capable of eliminating negative deviation interference in a creatinine determination caused by calcium dobesilate and etamsylate drugs in serum and a preparation method thereof. Key points of a technical scheme of the invention are that the creatinine kit includes a reagent R1 and a reagent R2, wherein the reagent R1 includes buffer, creatinase, sarcosineoxidase, ascorbic acid oxidase, peroxidase or catalase, serum albumin, nonionic surfactant, preservative, Trinder's reagent A, laccase or metalic acid salt; and the reagent R2 includes buffer, serum albumin, creatinine enzyme, preservative and Trinder's reagent B. The invention overcomes a defect of inaccurate creatinine determination due to the presence of the negative deviation caused by calciumdobesilate and etamsylat in serum samples when the clinical tests of creatinine determination are being carried out in various hospitals, and the invention is suitable for the detection of serum creatinine by an automatic biochemical analyzer.
Owner:ZHONGSHAN BGH BIOTECH CO LTD
Creatine amidinohydrolase, production thereof and use thereof
Owner:TOYOBO CO LTD
Reagent combination, reagent or kit for measuring creatinine content
InactiveCN111944872AReduce distractionsMicrobiological testing/measurementBiological material analysisCreatininasePeroxidase
The invention relates to the technical field of biology, and particularly relates to a reagent combination, a reagent or a kit for measuring creatinine content. The reagent combination comprises a reagent group 1 and a reagent group 2; the reagent group 1 comprises creatinase, sarcosine oxidase, ascorbic acid oxidase, chromogen and catalase; the reagent group 2 comprises creatinase, 4-aminoantipyrine and peroxidase; and the chromogen is selected from N-(2-hydroxy-3-sulfopropyl)-3, 5-dimethoxyaniline or sodium salt thereof and N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-5-dimethoxyaniline. An inhibitor is added into the measured reagent, so that generation of neocreatine and creatinine in the process of measuring is blocked, and interference existing in a sample in creatinine detection is lowered.
Owner:柏定生物工程(北京)有限公司
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