Reagent combination, reagent or kit for measuring creatinine content

Abstract

The invention relates to the technical field of biology, and particularly relates to a reagent combination, a reagent or a kit for measuring creatinine content. The reagent combination comprises a reagent group 1 and a reagent group 2; the reagent group 1 comprises creatinase, sarcosine oxidase, ascorbic acid oxidase, chromogen and catalase; the reagent group 2 comprises creatinase, 4-aminoantipyrine and peroxidase; and the chromogen is selected from N-(2-hydroxy-3-sulfopropyl)-3, 5-dimethoxyaniline or sodium salt thereof and N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-5-dimethoxyaniline. An inhibitor is added into the measured reagent, so that generation of neocreatine and creatinine in the process of measuring is blocked, and interference existing in a sample in creatinine detection is lowered.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a reagent combination, reagent or kit for measuring creatinine content. Background technique [0002] Creatinine is a low-molecular nitrogen compound with a molecular weight of 116,000, which is mainly produced by muscle metabolism. In muscle, creatine is mainly slowly formed into creatinine through irreversible non-enzymatic dehydration reaction, and then released into the blood and excreted with urine. Under normal circumstances, the creatinine content of the human body remains basically stable. The normal upper limit of plasma creatinine is around 100 micromol / L. Creatinine is a small molecular substance that can be filtered by the glomerulus, so the concentration change of serum creatinine is mainly determined by the filtration capacity of the glomerulus. As filtration capacity decreases, creatinine concentration increases. Serum creatinine values ​​that are higher than the n...

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In such a small state, the amount of newly produced creat

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