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Creatinine measurement reagent

A technology for reagents and creatinine, applied in the field of medical clinical testing, can solve the problems of insufficient stability of reagents and high price of creatinine imine hydrolase, and achieve the effects of eliminating instability of reagent components and stable and accurate test results.

Inactive Publication Date: 2014-05-07
深圳市雷诺华科技实业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the reagent is not stable enough, and the creatinine imine hydrolase used is expensive

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Reagent 1 used in this embodiment 1 includes:

[0055] N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid buffer (PH7.4) 25mmol / L

[0056]

[0057] Reagent 2 includes:

[0058] N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid buffer (PH7.6) 100mmol / L

[0059]

[0060] The test steps are as follows: Mix 6 ul of the sample (the calibrator is used as the sample during calibration) and 200 ul of the reagent; add 60 ul of reagent 2 after 5 minutes, and mix well. Read the absorbance at 340nm every 10-30 seconds, obtain the data of 10-20 points, and calculate △A 340 / min. Test the calibrator under the same conditions, and record the data to calculate the creatinine content of the tested sample.

Embodiment 2

[0062] Reagent 1 used in this embodiment 2 includes:

[0063] N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid buffer (PH7.4) 25mmol / L

[0064]

[0065] Reagent 2 includes:

[0066] N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid buffer (PH7.6) 100mmol / L

[0067]

[0068] The test steps are as follows: add 10ul of sample to 400ul of reagent 1, react at 37°C for 5 minutes; add 2100ul of reagent and mix well, read the absorbance A after 1 minute 1 ;Interval 2 minutes, read the absorbance A 2 . Use the calibrator instead of the sample for the same determination, and record the data to calculate the creatinine content of the tested sample.

Embodiment 3

[0070] Each composition and concentration range of reagent 1 are:

[0071] N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid buffer (PH8.0) 10mmol / L;

[0072]

[0073] Each composition and concentration range of reagent 2 are:

[0074] N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid buffer (PH8.5) 10mmol / L;

[0075]

[0076]

[0077] Creatinine measurement was performed following the same procedure as in Example 1 above.

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Abstract

The invention discloses a creatinine measurement reagent which comprises a reagent I and a reagent II, wherein the reagent I comprises 10-200mmol / L Good's buffering solution with the PH of 7.0-9.0, 5-120KU / L creatine amidine hydrolase, 2-50KU / L sarcosine oxidase, 1-10ml / L surfactant, 1-100mmol / L stabilizing agent and 0.1-50ml / l preservative; the reagent II comprises 10-200mmol / L Good's buffering solution with the PH of 7.0-9.0, 0.1-5mml / L NAD<+>, 3-200KU / L creatinine amidohydrolase, 1-50KU / L formaldehyde dehydrogenase, 1-10ml / L surfactant, 1-100mmol / L stabilizing agent and 0.1-50ml / l preservative. According to the creatinine measurement reagent disclosed by the invention, the problem of interference is solved; and the reagent components are stable.

Description

technical field [0001] The invention relates to the technical field of medical clinical testing, in particular to a method for measuring creatinine and a reagent for measuring creatinine. Background technique [0002] Creatinine is a low-molecular nitrogen compound with a molecular weight of 116,000, which is mainly produced by muscle metabolism. In muscle, creatine is mainly slowly formed into creatinine through irreversible non-enzymatic dehydration reaction, and then released into the blood and excreted with urine. Under normal circumstances, the creatinine content of the human body remains basically stable. The normal upper limit of plasma creatinine is around 100 micromol / L. Creatinine is a small molecular substance that can be filtered by the glomerulus, so the concentration change of serum creatinine is mainly determined by the filtration capacity of the glomerulus. As filtration capacity decreases, creatinine concentration increases. Serum creatinine values ​​tha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/34C12Q1/32C12Q1/26
Inventor 王荣光吕斌斌张继明
Owner 深圳市雷诺华科技实业有限公司
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