Dry test strip and method for measuring creatinine

A technology of creatinine and test piece, which is applied in the field of dry test piece for creatinine measurement and creatinine measurement method, and can solve the problems of low yield, large error in creatinine measurement, and inability to completely eliminate the influence

Active Publication Date: 2012-10-31
ARKRAY INC
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] However, the existing dry-type test piece for measuring creatinine using the Jiffy reaction shown in Japanese Patent Application Laid-Open No. 09-061430 has the disadvantage of large errors when measuring creatinine in a low concentration range.
[0009] The dry analysis element disclosed in Japanese Patent No. 4243255 has a smaller error when measuring creatine in a low concentration range than when using the Jiffy reaction, but has the following disadvantages: the influence of endogenous ammonia in the sample cannot be completely eliminated, In addition, complex layer structures are required to control ammonia gas as a reaction product, resulting in low yields for fabrication
[0010] In addition

Method used

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  • Dry test strip and method for measuring creatinine
  • Dry test strip and method for measuring creatinine
  • Dry test strip and method for measuring creatinine

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Embodiment approach

[0041] Such as Figure 1A and Figure 2A As shown, the dry test piece 1 for creatinine measurement according to the embodiment of the present invention is composed of a support 2 and a reagent part 3 supported on the support 2 . The reagent part 3 is composed of a reagent layer 4 formed on the support 2, a reagent holding layer 5 formed to cover the reagent layer 4, and a reagent layer formed between the reagent layer 4 and the reagent holding layer 5 for connecting the reagent layer. 4 and the connection layer 6 of the reagent holding layer 5 constitute.

[0042] The support 2 is preferably not liquid-permeable. Thus, when creatinine is measured using the dry test piece 1 for measuring creatinine according to this embodiment, the liquid sample dropped onto the reagent layer 4 and the reagent holding layer 5 stays in the reagent layer 4 and the reagent holding layer 5. , and therefore, can be measured with high precision. As the support 2, a paper sheet, a plastic (syntheti...

Embodiment 1

[0086] Hereinafter, examples are shown and the present invention will be described in more detail, but the present invention is not limited to the scope of the following examples. It should be noted that deionized water was used as pure water unless otherwise specified.

[0087] First, an elongated piece having a size of 70 mm in length x 5 mm in width was cut out from a white polyethylene terephthalate (PET) sheet having a thickness of 0.2 mm to prepare a support.

[0088] Next, 300 U of creatininase (manufactured by Kitsukoman Co., Ltd.), 81 mg of 4-aminoantipyrine (manufactured by Kishida Chemical Co., Ltd.), 435 mg of sodium alginate (manufactured by Nakalai Tesque) were dissolved in 22 ml of phosphate buffer (pH 7.5). Co., Ltd.). After stirring the aqueous solution well, it was degassed. The entire solution was applied to one end of the support in such a manner that it had a size of 7 mm in length×5 mm in width×0.1 mm in thickness. Then, it was dried at 40° C. for 20 m...

Embodiment 2

[0092] A support, a reagent layer, and a reagent holding layer were produced in the same manner as in Example 1. However, the reagent holding layer is not bonded to the reagent layer using an adhesive. Instead, the bottom surface of the reagent holding layer is wetted with pure water, laminated on the reagent layer, and dried to perform adhesion.

[0093] (reaction time course of creatine and creatinine)

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Abstract

A dry test strip for measuring creatinine (1) comprises: a support (2),a reagent layer (4) that is disposed on the support (2),a reagent holding layer (5) that is disposed on the reagent layer (4),and a connection layer (6) that is composed of an adhesive which adhesively bonds the reagent layer (4) to the reagent holding layer (5) and is formed in spot form, wherein the reagent layer (4) contains creatininase and 4-aminoantipyrine,the reagent holding layer (5) contains creatinase, sarcosine oxidase, peroxidase, and N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline,and the connection layer (6) delays arrival of a liquid sample spot-deposited on the reagent holding layer (5) at the reagent layer (4).

Description

technical field [0001] The present invention relates to a dry test piece for measuring creatinine and a method for measuring creatinine. Background technique [0002] Creatinine is the final metabolite of the creatine pathway, and creatine has a dehydrated ring structure. In the body, most is found in the muscles as creatine or phosphocreatine. Creatine receives high-energy phosphate from ATP to form phosphocreatine, which functions as an energy storage substance. Specifically, phosphocreatine transfers high-energy phosphoric acid to ADP during energy consumption such as muscle contraction to return to creatine, or forms creatinine through a non-enzymatic reaction. The creatinine produced is then excreted into urine through the kidneys. [0003] Therefore, the urinary excretion amount of creatinine, that is, the urinary concentration is used as an index of muscle diseases and renal dysfunction. In addition, in the case of renal dysfunction or the like, the creatinine con...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12Q1/34C12Q1/28C12Q1/26
CPCG01N33/70G01N33/526
Inventor 中村勤
Owner ARKRAY INC
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