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Creatinine kit for eliminating calcium dobesilate and etamsylate and preparation method thereof

A technology of calcium dobesilate and ethylamine sulfonate, which is applied in the field of medical testing, can solve the problems of inaccurate creatinine measurement and misjudgment, achieve precise medication and treatment, simple and rapid operation, and avoid misjudgment effects

Active Publication Date: 2020-10-02
ZHONGSHAN BGH BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The invention discloses a creatinine kit which can eliminate the negative deviation interference of creatinine measurement caused by calcium dobesilate and phensulfonate drugs in serum, and overcomes the problem of serum samples containing dobesilate when creatinine is measured in clinical tests in various hospitals. The negative deviation caused by calcium and phenetamine makes the measurement of creatinine inaccurate, which leads to the defect of misjudgment during the treatment of doctors. The kit has good stability, simple and fast operation, and accurate measurement. It is suitable for automatic biochemical analyzers. Serum creatinine detection

Method used

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  • Creatinine kit for eliminating calcium dobesilate and etamsylate and preparation method thereof
  • Creatinine kit for eliminating calcium dobesilate and etamsylate and preparation method thereof
  • Creatinine kit for eliminating calcium dobesilate and etamsylate and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Reagent R1 components:

[0063] TRIS buffer 50mM PH8.0,

[0064] Creatinase 25ku / L,

[0065] Sarcosine oxidase 15 ku / L,

[0066] Peroxidase 10 ku / L,

[0067] Ascorbate Oxidase 10 ku / L,

[0068] Laccase 1 ku / L,

[0069] 4-AAP 3mM,

[0070] Bovine Serum Albumin 0.1%,

[0071] TRITON X-100 0.2%,

[0072] PARABEN preservative 0.1%;

[0073] Reagent R2 components:

[0074] TRIS buffer 50mM PH8.0,

[0075] Creatinase 300 ku / L,

[0076] Bovine Serum Albumin 0.1%,

[0077] TOOS 10mM,

[0078] Sodium Azide 0.1%,

[0079] TRITON X-100 0.1%;

Embodiment 2

[0081] Reagent R1 components:

[0082] EPPS buffer 50mM PH8.0,

[0083] Creatinase 25 ku / L,

[0084] Sarcosine oxidase 15 ku / L,

[0085] Peroxidase 5.5ku / L,

[0086] Ascorbate oxidase 6.4ku / L,

[0087] Vanadium Pentoxide 1 mM,

[0088] 4-AAP 3 mM,

[0089] Bovine Serum Albumin 0.1%,

[0090] TRITON X-100 0.2%,

[0091] Chloramphenicol 0.1%;

[0092] Reagent R2 components:

[0093] EPPS buffer 50 mM pH8.0,

[0094] Creatinase 500 ku / L,

[0095] Bovine Serum Albumin 0.1%,

[0096] TOOS 10 mM,

[0097] Sodium Azide / Chloramphenicol 0.1%,

[0098] TRITON X-100 0.1%.

Embodiment 3

[0100] Reagent R1 components:

[0101] TRIS buffer 50mM PH8.0,

[0102] Creatinase 25 ku / L,

[0103] Sarcosine oxidase 15 ku / L,

[0104] Catalase 100ku / L,

[0105] Ascorbate oxidase 7.5ku / L,

[0106] Sodium ferrate 2 mM,

[0107] 4-AAP 3 mM,

[0108] Bovine Serum Albumin 0.1%,

[0109] TRITON X-100 0.2%,

[0110] Sodium azide 0.1%;

[0111] Reagent R2 components:

[0112] TRIS buffer 50 mM pH8.0,

[0113] Creatinase 500 ku / L,

[0114] Bovine Serum Albumin 0.1%,

[0115] TOOS 10 mM,

[0116] Chloramphenicol 0.1%,

[0117] TRITON X-100 0.1%.

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Abstract

The invention relates to a creatinine kit capable of eliminating negative deviation interference in a creatinine determination caused by calcium dobesilate and etamsylate drugs in serum and a preparation method thereof. Key points of a technical scheme of the invention are that the creatinine kit includes a reagent R1 and a reagent R2, wherein the reagent R1 includes buffer, creatinase, sarcosineoxidase, ascorbic acid oxidase, peroxidase or catalase, serum albumin, nonionic surfactant, preservative, Trinder's reagent A, laccase or metalic acid salt; and the reagent R2 includes buffer, serum albumin, creatinine enzyme, preservative and Trinder's reagent B. The invention overcomes a defect of inaccurate creatinine determination due to the presence of the negative deviation caused by calciumdobesilate and etamsylat in serum samples when the clinical tests of creatinine determination are being carried out in various hospitals, and the invention is suitable for the detection of serum creatinine by an automatic biochemical analyzer.

Description

technical field [0001] The invention belongs to the technical field of medical testing, and relates to a creatinine kit capable of eliminating calcium dobesilate and phenetamine and a preparation method thereof. Background technique [0002] Creatinine is the main index and detection item of clinical examination of upper renal function. There are two main methods for measuring serum creatinine: one is alkaline picric acid rate method (Jaffe method), the other is sarcosine oxidase method; Jaffe method Due to the narrow linear range and poor specificity, it is easily interfered by other substances in the sample (such as ketone bodies, ascorbic acid, bilirubin, cephalosporin, dopamine, etc.), and detection deviations have occurred, so it has gradually withdrawn from the market. At present, the sarcosine oxidase method is widely used in the market. This method has been gradually eliminated by various hospitals and replaced by the sarcosine oxidase method. The detection principle...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/26C12Q1/34C12Q1/28C12Q1/30
CPCC12Q1/26C12Q1/34C12Q1/28G01N2333/90683G01N2333/90235G01N2333/90232G01N2333/986C12Q2326/96G01N2333/902
Inventor 俞永标
Owner ZHONGSHAN BGH BIOTECH CO LTD
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