Creatinine kit for eliminating calcium dobesilate and etamsylate and preparation method thereof
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of calcium dobesilate and ethylamine sulfonate, which is applied in the field of medical testing, can solve the problems of inaccurate creatinine measurement and misjudgment, achieve precise medication and treatment, simple and rapid operation, and avoid misjudgment effects
Active Publication Date: 2020-10-02
ZHONGSHAN BGH BIOTECH CO LTD
View PDF6 Cites 4 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0008] The invention discloses a creatinine kit which can eliminate the negative deviation interference of creatinine measurement caused by calcium dobesilate and phensulfonate drugs in serum, and overcomes the problem of serum samples containing dobesilate when creatinine is measured in clinical tests in various hospitals. The negative deviation caused by calcium and phenetamine makes the measurement of creatinine inaccurate, which leads to the defect of misjudgment during the treatment of doctors. The kit has good stability, simple and fast operation, and accurate measurement. It is suitable for automatic biochemical analyzers. Serum creatinine detection
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0062] Reagent R1 components:
[0063] TRIS buffer 50mM PH8.0,
[0064] Creatinase 25ku / L,
[0065] Sarcosine oxidase 15 ku / L,
[0066] Peroxidase 10 ku / L,
[0067] Ascorbate Oxidase 10 ku / L,
[0068] Laccase 1 ku / L,
[0069] 4-AAP 3mM,
[0070] Bovine Serum Albumin 0.1%,
[0071] TRITON X-100 0.2%,
[0072] PARABEN preservative 0.1%;
[0073] Reagent R2 components:
[0074] TRIS buffer 50mM PH8.0,
[0075] Creatinase 300 ku / L,
[0076] Bovine Serum Albumin 0.1%,
[0077] TOOS 10mM,
[0078] Sodium Azide 0.1%,
[0079] TRITON X-100 0.1%;
Embodiment 2
[0081] Reagent R1 components:
[0082] EPPS buffer 50mM PH8.0,
[0083] Creatinase 25 ku / L,
[0084] Sarcosine oxidase 15 ku / L,
[0085] Peroxidase 5.5ku / L,
[0086] Ascorbate oxidase 6.4ku / L,
[0087] Vanadium Pentoxide 1 mM,
[0088] 4-AAP 3 mM,
[0089] Bovine Serum Albumin 0.1%,
[0090] TRITON X-100 0.2%,
[0091] Chloramphenicol 0.1%;
[0092] Reagent R2 components:
[0093] EPPS buffer 50 mM pH8.0,
[0094] Creatinase 500 ku / L,
[0095] Bovine Serum Albumin 0.1%,
[0096] TOOS 10 mM,
[0097] Sodium Azide / Chloramphenicol 0.1%,
[0098] TRITON X-100 0.1%.
Embodiment 3
[0100] Reagent R1 components:
[0101] TRIS buffer 50mM PH8.0,
[0102] Creatinase 25 ku / L,
[0103] Sarcosine oxidase 15 ku / L,
[0104] Catalase 100ku / L,
[0105] Ascorbate oxidase 7.5ku / L,
[0106] Sodium ferrate 2 mM,
[0107] 4-AAP 3 mM,
[0108] Bovine Serum Albumin 0.1%,
[0109] TRITON X-100 0.2%,
[0110] Sodium azide 0.1%;
[0111] Reagent R2 components:
[0112] TRIS buffer 50 mM pH8.0,
[0113] Creatinase 500 ku / L,
[0114] Bovine Serum Albumin 0.1%,
[0115] TOOS 10 mM,
[0116] Chloramphenicol 0.1%,
[0117] TRITON X-100 0.1%.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention relates to a creatinine kit capable of eliminating negative deviation interference in a creatinine determination caused by calcium dobesilate and etamsylate drugs in serum and a preparation method thereof. Key points of a technical scheme of the invention are that the creatinine kit includes a reagent R1 and a reagent R2, wherein the reagent R1 includes buffer, creatinase, sarcosineoxidase, ascorbic acid oxidase, peroxidase or catalase, serum albumin, nonionic surfactant, preservative, Trinder's reagent A, laccase or metalic acid salt; and the reagent R2 includes buffer, serum albumin, creatinine enzyme, preservative and Trinder's reagent B. The invention overcomes a defect of inaccurate creatinine determination due to the presence of the negative deviation caused by calciumdobesilate and etamsylat in serum samples when the clinical tests of creatinine determination are being carried out in various hospitals, and the invention is suitable for the detection of serum creatinine by an automatic biochemical analyzer.
Description
technical field [0001] The invention belongs to the technical field of medical testing, and relates to a creatinine kit capable of eliminating calcium dobesilate and phenetamine and a preparation method thereof. Background technique [0002] Creatinine is the main index and detection item of clinical examination of upper renal function. There are two main methods for measuring serum creatinine: one is alkaline picric acid rate method (Jaffe method), the other is sarcosine oxidase method; Jaffe method Due to the narrow linear range and poor specificity, it is easily interfered by other substances in the sample (such as ketone bodies, ascorbic acid, bilirubin, cephalosporin, dopamine, etc.), and detection deviations have occurred, so it has gradually withdrawn from the market. At present, the sarcosine oxidase method is widely used in the market. This method has been gradually eliminated by various hospitals and replaced by the sarcosine oxidase method. The detection principle...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more