Creatinine detection kit and use method thereof

Abstract

The invention provides a creatinine detection kit and a use method thereof. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises creatinase, sarcosine oxidase, peroxidase,ascorbic acid oxidase, 2, 4, 6-tribromo-3-hydroxybenzoic acid and 3, 5-dichloro-2-hydroxybenzenesulfonic acid; and the reagent R2 comprises creatinase, peroxidase, potassium ferrocyanide and 4-aminoantipyrine. The creatinine detection kit provided by the invention can resist interference of etamsylate with the concentration of 300mg/L (the highest blood concentration) or below in a to-be-detectedsample, has a wider linear range (0-6000 millimoles/L), is applicable to detection of samples with high creatinine content and can correctly evaluate renal functions of surgical patients.

Description

technical field [0001] The invention relates to the technical field of creatinine detection, in particular to a creatinine detection kit and a use method thereof. Background technique [0002] Creatinine (creatinine, Cre) is a low molecular weight nitrogenous compound, which is a metabolite of muscle in the human body. Serum creatinine is commonly used in the clinical detection of advanced renal disease. The methods for measuring creatinine mainly include chemical method and enzymatic method. The chemical method is mainly picric acid method, which has poor specificity; there are three main enzymatic methods: creatinine aminohydrolase method, sarcosine oxidase method, and creatinine iminohydrolase method. . The enzymatic method has better specificity and can eliminate the interference of endogenous creatinine. [0003] At present, the creatinine detection kit widely used in clinic generally adopts the enzyme coupling method, based on the Trinder reaction, the principle is ...

AI Technical Summary

Problems solved by technology

[0005] Therefore, the technical problem to be solved in the present invention is to overcome the small linear detection range of the creatinine detection kit of the resistance to the interference of ethanamine in the prior art, and the defect that samples with high creatinine content cannot be accurately measured, thereby providing a kind of Creatinine detection kit with a wide linear range and anti-interference with phenetamine

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