Creatinine assay kit and application thereof

A technology of kits and reagents, applied in biological testing, color/spectral characteristic measurement, and analysis through chemical reactions of materials, etc., can solve problems such as narrow linear range and poor stability, and achieve simple components and increased stability , the effect of high accuracy

Active Publication Date: 2019-04-12
浙江夸克生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aiming at the defects of narrow linear range and poor stability in the process of creatinine content determination in the prior art, the present invention provides a creatin

Method used

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  • Creatinine assay kit and application thereof
  • Creatinine assay kit and application thereof
  • Creatinine assay kit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] The components and concentrations of the creatinine assay kit of the present embodiment are as follows:

[0058] Reagent R1 includes: creatinase 10.0KU / L, catalase 5.0KU / L, sarcosine oxidase 50.0KU / L, 4-aminoantipyrine 1.0mmol / L, hydroxylamine 0.1g / L, pH 3-morpholinepropanesulfonic acid buffer solution with a value of 7.0 50.0mmol / L and yeast extract 0.2g / L; reagent R2 includes: creatinase 100.0KU / L, peroxidase 1.0KU / L, phenol 1.0mmol / L L, 2.0 KU / L of ascorbate oxidase, 0.1 g / L of hydroxylamine, 50.0 mmol / L of 3-morpholine propanesulfonic acid buffer solution with a pH value of 7.0 and 0.2 g / L of yeast extract.

Embodiment 2

[0060] The components and concentrations of the creatinine assay kit of the present embodiment are as follows:

[0061] Reagent R1 includes: creatinase 20.0KU / L, catalase 30.0KU / L, sarcosine oxidase 100.0KU / L, 4-aminoantipyrine 10.0mmol / L, hydroxylamine 3.0g / L, pH 3-morpholinepropanesulfonic acid buffer solution with a value of 7.0 100.0mmol / L and yeast extract 3.0g / L; reagent R2 includes: creatinase 200.0KU / L, peroxidase 10.0KU / L, phenol 10.0mmol / L L, 15.0 KU / L of ascorbate oxidase, 3.0 g / L of hydroxylamine, 100.0 mmol / L of 3-morpholine propanesulfonic acid buffer solution with a pH value of 7.0 and 3.0 g / L of yeast extract.

Embodiment 3

[0063] The components and concentrations of the creatinine assay kit of the present embodiment are as follows:

[0064] Reagent R1 includes: creatinase 12.0KU / L, catalase 8.0KU / L, sarcosine oxidase 60.0KU / L, 4-aminoantipyrine 2.0mmol / L, fluoride 0.5g / L, Borate buffer solution with a pH value of 7.0 60.0mmol / L and yeast extract 0.5g / L; Reagent R2 includes: creatinase 130.0KU / L, peroxidase 3.0KU / L, 4-chlorophenol 2.0mmol / L L, ascorbate oxidase 5.0KU / L, fluoride 0.5g / L, borate buffer solution 60.0mmol / L with a pH value of 7.0 and yeast extract 0.5g / L.

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Abstract

The invention relates to a creatinine assay kit and application thereof, and belongs to the technical field of biological detection. The creatinine assay kit is composed of a reagent R1 and a reagentR2; the reagent R1 is prepared from creatinase, catalase, sarcosine oxidase, 4-amino-antipyrine, an inhibitor and a buffer solution; and the reagent R2 is prepared from creatinase, catalase, a color developing agent, ascorbic acid oxidase, an inhibitor and a buffer solution. According to the creatinine assay kit, assaying is conducted through a sarcosine oxidase method, and the creatinine assay kit has the advantages of wide linear range, good stability and the like.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a creatinine measurement kit and an application thereof. Background technique [0002] Creatinine is the final product of creatine and phosphocreatine metabolism in muscle, excreted only by glomeruli, not reabsorbed by renal tubules, and usually not bound to plasma proteins. When the kidney function is impaired, the normal excretion of creatinine is hindered, resulting in an increase in serum creatinine content. The incidence of kidney disease ranks among the top 10 in my country and even in the world. According to survey data, patients with invisible kidney disease account for about 25% of the population in my country, so the determination of creatinine level has important clinical significance for kidney disease. [0003] As a small molecular protein metabolite, creatinine has a very low content in human serum and coexists with its parent compound cre...

Claims

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Application Information

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IPC IPC(8): G01N33/50G01N21/31G01N21/75
CPCG01N21/31G01N21/75G01N33/50
Inventor 李传清钟素萍
Owner 浙江夸克生物科技有限公司
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