Dry chemical reagent tablet for quantitatively measuring concentration of creatinine and preparation method of dry chemical reagent tablet

Abstract

The invention belongs to the technical field of clinical in vitro diagnostic reagents and relates to a dry chemical reagent tablet for quantitatively measuring the concentration of creatinine and a preparation method of the dry chemical reagent tablet. The dry chemical reagent tablet comprises four laminations which are respectively a support layer, a color developing layer, a reagent layer and adiffusion layer distributed sequentially; and reagents in the reagent layer include a buffer system, a hydrophilic colloid, a creatine amidinohydrolase, sarcosine oxidase, peroxidase, a protective agent, a chelating agent, an activator and a surfactant; and reagents in the color developing layer include a chromogen, a creatine amidohydrolase, a hydrophilic colloid, and a surfactant. The dry chemical reagent tablet and the preparation method thereof of the invention have the advantages of simple operation process, high stability, high good repeatability and high accuracy of detection results, quantitative detection, wide linear range, simple and convenient preparation process, easiness in operation, low risk and low pollution. According to the dry chemical reagent tablet and the preparationmethod of the dry chemical reagent tablet of the invention, the reagents do not need to be prepared, and samples can be directly added dropwise; and the reagent tablet is dry and is free of moisture;the diffusion layer has a filtering function, so that hemolysis, bilirubin and ascorbic acid samples exert no significant interference on measurement results.

Description

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AI Technical Summary

Benefits of technology

This patented technology offers an improved way to measure concentrations of substances like creatins or cytokines by measuring their effect on certain enzymes found within them. It simplifies operations while maintaining accurate measurements over time without causing damage from environmental factors such as humid air or water. Additionally, it allows for precise control during manufacturment processes, ensures consistency between different samples, improves reproducibility, reliability, durability, ease of use, and reduces risk of contamination. Overall, this new technique makes researchers more efficient ways to study biological systems faster than current methods.

Problems solved by technology

Technological Problem: Current Method For Detecting Cysteaminaemia Using Chemilumin® Assay involves collecting samples containing cytosmium protein called troponin I during exercise training, followed by performing various assays like immunochemistry, histopathology, and necrosis techniques. However these existing diagnoses rely heavily on laborious procedures involving specialized devices and may result in biomarker fluctuates due to changes caused by different conditions including physical activity levels and nutritional status. Additionally, there exist non-standard ways to determine how much calcium per kilogram unit (CMP) should be removed without causing damage.

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