Preparation method of cell wax block as well as product and application of cell wax block

A technology of cell wax blocks and exfoliated cells, which is applied in the field of biomedicine, can solve the problems of poor cell clustering effect, cumbersome preparation steps, and low efficiency, and achieve the effect of intact cell shape, clear observation background, and high aggregation

Pending Publication Date: 2022-04-08
GUANGZHOU LBP MEDICINE SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this method contains more reagents, the preparation steps are cumbersome, and the efficiency is low
[0008] Based on the above research, it can be seen that the preparation method of the traditional cell wax block has the problems of cumbersome reagent preparation, complicated operation, low efficiency and poor cell agglomeration effect.

Method used

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  • Preparation method of cell wax block as well as product and application of cell wax block
  • Preparation method of cell wax block as well as product and application of cell wax block
  • Preparation method of cell wax block as well as product and application of cell wax block

Examples

Experimental program
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Effect test

Embodiment 1

[0077] This embodiment provides a method for preparing a cell wax block, comprising the following steps:

[0078] (1) Take 12 mL of cervical cell sample in a centrifuge tube, centrifuge at 800 g for 10 min, discard the supernatant, and obtain the first precipitate;

[0079] (2) Mix egg white powder with 0.9wt% Nacl solution at a ratio of 1:16g / mL, shake for 30s to obtain Nacl egg white solution;

[0080] (3) Add the Nacl egg white solution obtained in step (2) to the first precipitate obtained in step (1) in a ratio of 1:1.5g / mL, and mix well to obtain a suspension;

[0081] (4) Add 80wt% ethanol solution in the ratio of 1:1g / mL to the suspension obtained in step (3), let it stand for 5min, centrifuge at 600g for 10min, pour off the supernatant, and obtain the second precipitate;

[0082] (5) Add 1 mL of formalin to the second precipitate obtained in step (4), let it stand for 15 minutes, take out the second precipitate, put it in a dehydrator for dehydration, take it out aft...

Embodiment 2

[0084] This embodiment provides a method for preparing a cell wax block, comprising the following steps:

[0085] (1) Take 12 mL of thyroid puncture cell sample in a centrifuge tube, centrifuge at 600 g for 8 min, discard the supernatant, and obtain the first precipitate;

[0086] (2) Mix egg white powder with 0.9wt% Nacl solution at a ratio of 1:24g / mL, shake for 25s to obtain Nacl egg white solution;

[0087] (3) Add the Nacl egg white solution obtained in step (2) to the first precipitate obtained in step (1) in a ratio of 1:1.5g / mL, and mix well to obtain a suspension;

[0088] (4) Add 80wt% ethanol solution in the ratio of 1:1g / mL to the suspension obtained in step (3), let it stand for 4min, centrifuge at 700g for 10min, pour off the supernatant, and obtain the second precipitate;

[0089] (5) Add 0.8mL formalin to the second precipitate obtained in step (4), let it stand for 12 minutes, take out the second precipitate and put it in a dehydrator for dehydration. After t...

Embodiment 3

[0091] This embodiment provides a method for preparing a cell wax block, comprising the following steps:

[0092] (1) Take 50mL serosal cavity cell sample in a centrifuge tube, centrifuge at 550g for 15min, discard the supernatant, and obtain the first precipitate;

[0093] (2) Mix egg white powder with 0.9wt% Nacl solution at a ratio of 1:16g / mL, shake for 40s to obtain Nacl egg white solution;

[0094] (3) Add the Nacl egg white solution obtained in step (2) to the first precipitate obtained in step (1) in a ratio of 1:1.5g / mL, and mix well to obtain a suspension;

[0095] (4) Add 80wt% ethanol solution in the ratio of 1:1g / mL to the suspension obtained in step (3), let it stand for 8min, centrifuge at 550g for 12min, pour off the supernatant, and obtain the second precipitate;

[0096] (5) Add 1.2mL formalin to the second precipitate obtained in step (4), let it stand for 8 minutes, take out the second precipitate, put it into a dehydrator for dehydration, take it out afte...

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Abstract

The invention provides a preparation method of a cell wax block as well as a product and application of the cell wax block. The preparation method comprises the following steps: (1) centrifuging an exfoliated cell sample to obtain a first precipitate; (2) uniformly mixing the first precipitate obtained in the step (1) with a Nacl egg white solution to obtain a suspension, then mixing an ethanol solution with the suspension, standing and centrifuging to obtain a second precipitate; and (3) treating the second precipitate obtained in the step (2) with formalin, standing, then taking out the second precipitate, dehydrating and embedding to obtain the cell wax block. The cell wax block obtained by the preparation method has enough cells, and the cells are intact in morphology, high in aggregation, good in clustering property, capable of being continuously sliced, clear in film reading background and capable of meeting the requirements of subsequent detection. In addition, the cell wax block can be used for preparing a disease diagnosis device.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a preparation method of a cell wax block and its product and application. Background technique [0002] Exfoliative cytology is a clinical laboratory discipline that collects exfoliated cells from various parts of the human body, especially the surface of luminal organs, observes the morphology of these cells with a microscope after staining, and makes a diagnosis, also known as diagnostic cytology or clinical cytology. With the advantages of fast, accurate, convenient and less pain for patients, it has become one of the important means of clinical diagnosis. [0003] At present, liquid-based cytology technology, a technology that preserves exfoliated cells in liquid and evenly disperses and attaches the cells on glass slides to make smears through special equipment, is widely used in gynecological cervical cytology examination technology. Later, the technology was also ap...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
Inventor 王丹宇宁贤霜
Owner GUANGZHOU LBP MEDICINE SCI & TECH
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