A kind of lipase detection kit and production process

A technology for detection kits and production processes, applied in measurement devices, analysis by chemical reaction of materials, and material analysis by observing the impact on chemical indicators, which can solve the problems of poor stability and storage time affected by environmental conditions. , Serum LPS activity is complex and other issues, to achieve good stability

Active Publication Date: 2021-07-20
NANJING AUBRIME ABM BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In several existing technologies, the determination of serum LPS activity requires a relatively complicated liquid preparation process, and the storage time is greatly affected by environmental conditions, and the stability of long-term storage is poor.

Method used

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  • A kind of lipase detection kit and production process
  • A kind of lipase detection kit and production process
  • A kind of lipase detection kit and production process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1: the production of lipase detection kit comprises the following steps:

[0043] S1: Weigh each component of reagent R1, add it into the solvent and stir, stir evenly, let it stand still, filter and remove impurities, and the content of each component is as follows:

[0044]

[0045] S2: Weigh each component of reagent R2, add it into the solvent and stir, stir evenly, let it stand still, filter and remove impurities, and the content of each component is as follows:

[0046]

[0047]

[0048] S3: inspection of semi-finished products;

[0049] S4: Clean the packaging utensils, dry them, and fill the reagents R1 and R2 respectively;

[0050] S5: Packaging, finished product inspection, storage in warehouse.

[0051] Wherein step S1, step S2 and step S4 are all carried out in a 100,000-class purification workshop.

Embodiment 2

[0052] Embodiment 2: the production of lipase detection kit comprises the following steps:

[0053] S1: Weigh each component of reagent R1, add it into the solvent and stir, stir evenly, let it stand still, filter and remove impurities, and the content of each component is as follows:

[0054]

[0055] S2: Weigh each component of reagent R2, add it into the solvent and stir, stir evenly, let it stand still, filter and remove impurities, and the content of each component is as follows:

[0056]

[0057] S3: inspection of semi-finished products;

[0058] S4: Clean the packaging utensils, dry them, and fill the reagents R1 and R2 respectively;

[0059] S5: Packaging, finished product inspection, storage in warehouse.

[0060] Wherein step S1, step S2 and step S4 are all carried out in a 100,000-class purification workshop.

Embodiment 3

[0061] Embodiment 3: the production of lipase detection kit comprises the following steps:

[0062] S1: Weigh each component of reagent R1, add it into the solvent and stir, stir evenly, let it stand still, filter and remove impurities, and the content of each component is as follows:

[0063]

[0064] S2: Weigh each component of reagent R2, add it into the solvent and stir, stir evenly, let it stand still, filter and remove impurities, and the content of each component is as follows:

[0065]

[0066] S3: inspection of semi-finished products;

[0067] S4: Clean the packaging utensils, dry them, and fill the reagents R1 and R2 respectively;

[0068] S5: Packaging, finished product inspection, storage in warehouse.

[0069] Wherein step S1, step S2 and step S4 are all carried out in a 100,000-class purification workshop.

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Abstract

A lipase detection kit of the present invention includes reagent R1 and reagent R2 which are independent of each other. Reagent R1 includes the following components: Reagent R2 includes the following components: the lipase detection kit is ready-to-use, does not need to be prepared, and is stored for a long time Good stability. The invention also proposes a production process of the lipase detection kit.

Description

technical field [0001] The invention relates to a lipase detection kit, which belongs to the technical field of diagnostic kits. Background technique [0002] Lipase (Lipase, LPS) is a group of lipolytic enzymes with low specificity, mainly derived from the pancreas, followed by the stomach and small intestine, and can hydrolyze a variety of glycerides containing long-chain fatty acids. Usually the pancreas secretes lipase and co-lipase into the circulation in equal amounts, but co-lipase can be filtered out from the glomerulus because of its relatively small molecular weight. In acute pancreatitis, the co-lipase / lipase ratio decreases. [0003] The determination of serum LPS activity is an important indicator for the diagnosis of acute pancreatitis. In acute pancreatitis, serum amylase increases for a short period of time, while serum LPS activity increases for 10-15 days. In addition, chronic pancreatitis, obstruction of the pancreatic duct by stones, liver disease, surg...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N21/01
CPCG01N21/01G01N21/78
Inventor 曾宪亮路爱成宣善浪
Owner NANJING AUBRIME ABM BIOTECH
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