Detection kit for determining content of creatinine in serum by enzymic method

A technology for detecting kits and reagents, which is applied in the measurement of color/spectral characteristics, material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions, etc., which can solve the problems of affecting the measurement results, etc. Achieve the effects of easy promotion and use, good stability and long storage time

Inactive Publication Date: 2014-12-10
上海睿康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method can complete the entire reaction in only two steps, the endogenous ammonia in the sample becomes the main interference factor and affects the measurement results.

Method used

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  • Detection kit for determining content of creatinine in serum by enzymic method
  • Detection kit for determining content of creatinine in serum by enzymic method
  • Detection kit for determining content of creatinine in serum by enzymic method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The composition of the assay kit is as follows:

[0034] 1. Reagent R1 is:

[0035]

[0036] 2. Reagent R2 is:

[0037]

[0038] Components can be added sequentially at room temperature, added simultaneously, or individually packaged and reconstituted immediately prior to testing.

[0039] 3. The reagent detection ratio is 3:1

[0040] The CRE detection kit described in this example is applicable to various types of automatic biochemical analyzers, taking Hitachi 7170 automatic biochemical analyzer as an example, its operation is shown in Table 1. Analysis method: Two-point endpoint method, that is, the dosage of reagents R1 and R2 are 240ul and 80ul respectively, and the sample volume is 5ul; add 5ul sample to 240ul reagent R1 and incubate at 37°C for 5min, and read the absorbance A 0 , add 80ul R2, incubate at 37°C for 5min, read the absorbance A 1 , calculate ΔA=A 1 -A 0 ; The main detection wavelength is 546nm, and the secondary wavelength is 700nm.

[...

Embodiment 2

[0046] The composition of the assay kit is as follows:

[0047] 1. Reagent R1 is:

[0048]

[0049] 2. Reagent R2 is:

[0050]

[0051] Components can be added sequentially at room temperature, added simultaneously, or individually packaged and reconstituted immediately prior to testing.

[0052] 3. The reagent detection ratio is 3:1

[0053] The CRE detection kit described in this example is applicable to various types of automatic biochemical analyzers, taking Hitachi 7170 automatic biochemical analyzer as an example, its operation is shown in Table 1. Analysis method: Two-point endpoint method, that is, the dosage of reagents R1 and R2 are 240ul and 80ul respectively, and the sample volume is 5ul; add 5ul sample to 240ul reagent R1 and incubate at 37°C for 5min, and read the absorbance A 0 , add 80ul R2, incubate at 37°C for 5min, read the absorbance A 1 , calculate ΔA=A 1 -A 0 ; The main detection wavelength is 546nm, and the secondary wavelength is 700nm.

Embodiment 3

[0055] The composition of the assay kit is as follows:

[0056] 1. Reagent R1 is:

[0057]

[0058] 2. Reagent R2 is:

[0059]

[0060] Components can be added sequentially at room temperature, added simultaneously, or individually packaged and reconstituted immediately prior to testing.

[0061] 3. The reagent detection ratio is 3:1

[0062] The CRE detection kit described in this example is applicable to various types of automatic biochemical analyzers, taking Hitachi 7170 automatic biochemical analyzer as an example, its operation is shown in Table 1. Analysis method: Two-point endpoint method, that is, the dosage of reagents R1 and R2 are 240ul and 80ul respectively, and the sample volume is 5ul; add 5ul sample to 240ul reagent R1 and incubate at 37°C for 5min, and read the absorbance A 0 , add 80ul R2, incubate at 37°C for 5min, read the absorbance A 1 , calculate ΔA=A 1 -A 0 ; The main detection wavelength is 546nm, and the secondary wavelength is 700nm.

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Abstract

The invention discloses a detection kit for determining the content of creatinine in serum by an enzymic method. The detection kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 contains 3-10g / L of buffering solution with the pH value being 7.5-8.0, 0.2-0.5g / L of EDTA, 0.5-2g / L of N-ethyl-N-(2-hydroxyl-3-sulfopropyl) m-toluidine sodium salt, 1 per mill-5 per mill of a surface active agent, 0.2-1g / L of sarcosine oxidase, 1-5KU / L of ascorbate oxidase, 2-5KU / L of creatinase and 0.5-1g / L of a stabilizing agent; the reagent R2 contains 3-10g / L of buffering solution with the pH value being 7.5-8.0, 0.1-0.5g / L of 4-aminoantipyrine, 0.1-0.4g / L of potassium ferricyanide, 2-8g / L of creatininase amidohydrolase, 1-6KU / L of peroxidase and 0.5-2g / L of a preservative. The detection kit disclosed by the invention is excellent in stability, strong in interference resistance and high in clinical application value.

Description

technical field [0001] The invention relates to a detection kit for measuring creatinine (CRE) content in serum by enzymatic method. Background technique [0002] Creatinine (creatinine, Cre) is the product of muscle metabolism in the human body, and every 20g of muscle metabolism can produce 1mg of creatinine. Blood creatinine comes from both exogenous and endogenous sources. Exogenous creatinine is the product of meat food metabolism in the body; endogenous creatinine is the product of muscle tissue metabolism in the body. When the intake of meat food is stable and the body's muscle metabolism does not change significantly, the production of creatinine will be relatively constant. [0003] The determination of serum creatinine concentration is an effective indicator for evaluating glomerular filtration rate (GFR). In acute renal failure and chronic progressive renal failure, the determination of serum creatinine is helpful for clinical judgment of the disease, especially ...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/78
Inventor 李伟奇陈瑛房君江张秀文林清玉
Owner 上海睿康生物科技有限公司
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