102 results about "L-Ascorbate" patented technology

The invention relates to a fluorescence biosensor, and a preparation method and a purpose thereof. On the basis of a two-dimensional manganese dioxide nanometer sheet as a fluorescence sensing platform, morpholineethanesulfonic acid is used for reducing potassium permanganate for synthesizing the manganese dioxide nanometer sheets; the manganese dioxide nanometer sheets adsorb FAM-ssDNA and quench FAM fluorescence; under the effect of alkaline phosphatase, vitamin C magnesium L-ascorbate-2-phosphate is hydrolyzed to generate ascorbic acid; the two-dimensional manganese dioxide nanometer sheets are reduced into manganese ions by the ascorbic acid; FAM-ssDNA is released; the fluorescence is recovered; the fluorescence sensor using the manganese dioxide nanometer sheets and the alkaline phosphatase to regulate the fluorescence intensity is built and is used for detecting the activity of the alkaline phosphatase. Compared with the prior art, the fluorescence biosensor has the characteristics of simplicity, high speed, low background signal, high sensitivity, low detection limit and good selectivity by using the change of the two-dimensional manganese dioxide nanometer sheets on the fluorescence signal, i.e., the off-on mode for detecting the alkaline phosphatase.

The invention discloses a detection kit for determining the content of creatinine in serum by an enzymic method. The detection kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 contains 3-10g/L of buffering solution with the pH value being 7.5-8.0, 0.2-0.5g/L of EDTA, 0.5-2g/L of N-ethyl-N-(2-hydroxyl-3-sulfopropyl) m-toluidine sodium salt, 1 per mill-5 per mill of a surface active agent, 0.2-1g/L of sarcosine oxidase, 1-5KU/L of ascorbate oxidase, 2-5KU/L of creatinase and 0.5-1g/L of a stabilizing agent; the reagent R2 contains 3-10g/L of buffering solution with the pH value being 7.5-8.0, 0.1-0.5g/L of 4-aminoantipyrine, 0.1-0.4g/L of potassium ferricyanide, 2-8g/L of creatininase amidohydrolase, 1-6KU/L of peroxidase and 0.5-2g/L of a preservative. The detection kit disclosed by the invention is excellent in stability, strong in interference resistance and high in clinical application value.

A method for detecting the As in food, health-care food, or biological specimen includes dissolving the specimen in nitric acid and hydrogenperoxide mixture under action of microwave, adding the mixed solution of 150 g/L thiourea and 100g/L VC, mixing, taking 25ml solution laying aside at orinary temp for 40-50 min, and atomic spectrofluorimetry. Its advantages are high speed, correctness and sensitivity.

The invention discloses a kit for detecting 1, 5-anhydro-D-ghlcitol in human blood. R1 comprises components as follows: 20-200 mM of a buffer solution, 1-10 mM of 4-aminoantipyrine, 0.2-2 mM of ATP (adenosine triphosphate), 1-10 mM of PEP (phosphoenolpyruvate), 1-50 KU/L of ATP-HK, 1-50 KU/L of pyruvate kinase, 1-50 KU/L of ascorbic acid oxidase, 0.05-10 mM of sodium vanadate, 0.01%-5% of a surface active agent, 0.1%-10% of a stabilizer and 0.01%-1% of a preservative; and R2 comprises components as follows: 20-200 mM of a buffer solution, 1-10 mM of TOOS (N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline,sodium salt), 1-100 KU/L of POD (peroxidase), 10-200 KU/L of PROD (pyranose oxidase), 0.01%-5% of a surface active agent, 0.1%-10% of a stabilizer and 0.01%-1% of a preservative. The kit has the advantages that endogenous interference of glucose, bilirubin, ascorbic acid, blood lipid and the like is effectively prevented and the stability is good.

The present invention provides compound feed for the polyculture of Litopenaeus vannamei and Trachinotus ovatus and a preparation method thereof. The compound feed is characterized by comprising the following ingredients in parts by weight: 15-25 parts of fish meal, 5-10 parts of meat and bone meal, 1-5 parts of maggot powder, 15-25 parts of soybean meal, 5-10 parts of peanut bran, 1-5 parts of soybean protein concentrate, 1-5 parts of cottonseed meal, 1-5 parts of corn gluten meal, 1-3 parts of molasses yeast, 1-3 parts of corn DDGS, 1-3 parts of squid paste, 1-3 parts of shrimp shell powder, 20-25 parts of high-gluten flour, 1-2 parts of sea fish oil, 1-2 parts of soybean oil, 1-2 parts of soy lecithin, 0.5-1 parts of calcium dihydrogen phosphate, 0.1-0.3 parts of choline chloride, 0.1-0.3 parts of cholesterol, 0.05-0.1 parts of L-ascorbate-2-monophosphate, 0.1-0.3 parts of coated lysine, 0.1-0.3 parts of coated methionine, 0.1-0.3 parts of Bacillus subtilis, 0.1-0.3 parts of mannan, 0.1-0.5 parts of complex vitamins, 0.1-0.5 parts of complex mineral salts. Experiments show that the compound feed for the polyculture of Litopenaeus vannamei and Trachinotus ovatus of the present invention can effectively meet the comprehensive nutrient needs of both Litopenaeus vannamei and Trachinotus ovatus, significantly improves the effective utilization of the feed, and has a relatively low food coefficient.

The invention belongs to the technical field of graphene oxide, particularly relates to a method for preparing small graphene from aphanitic graphite, and mainly aims to solve the problems of low purity, high toxicity, complex process and high cost of an existing graphene oxide preparation method. The preparation method for the small graphene comprises the following steps of uniformly mixing purified aphanitic graphite, concentrated acid and a strong oxidizer, performing reaction in an airtight environment of 80 to 120 DEG C, slowly pouring a reaction mixture into excessive de-ionized water, performing uniform stirring, adding an aqueous solution of hydrogen peroxide until suspension is brown yellow or luminous yellow, performing acid washing and water washing until the pH value is 6 to 7, performing centrifugal separation to obtain small graphene oxide, extracting an aqueous solution of L-ascorbate according to the mass ratio of L-ascorbate to the small graphene oxide being 1:(0.1-100), and performing reaction for 1min to 5h at the temperature of 40 to 100 DEG C to obtain the graphene.

The invention discloses a novel high-calcium nutrient protein powder and a preparation method thereof. The novel high-calcium nutrient protein powder is prepared from the following raw materials in percentage by weight: 20-40% of whole milk powder, 5-20% of maltodextrin, 1-10% of xylitol, 0.2-5.0% of isomaltooligosaccharide powder, 0.2-2.0% of calcium carbonate, 0-1.0% of silicon dioxide, 0.05-0.50% of sodium L-ascorbate, 0-0.02% of Bifidobacterium lactis Bi-07, 0-0.02% of Bifidobacterium animalis Bb-12, 35-55% of compound protein composition and 0.01-0.10% of complex vitamin premix. The novel high-calcium nutrient protein powder has the advantages of high calcium content and comprehensive and reasonable amino acid content. The added probiotics effectively promote the proliferation of beneficial bacteria in the intestinal tract of the human body and improve the intestinal tract problem. The added sodium L-ascorbate has the functions of resisting oxidation, postponing aging and enhancing the immunity.

The present invention discloses a feeder-layer-free human pluripotency stem cell culture medium, which contains L-ascorbate-2 magnesium phosphate, sodium selenite, recombinant human insulin, human apotransferrin, basic fibroblast growth factor, transforming growth factor beta 1, heparin lithium and/or heparin sodium, a DMEM/F-12 base culture medium and an osmotic pressure regulator. According to the present invention, with the culture medium, when the hiPSC culture is performed in low density and normal density, the proliferation rate is high, and the cell morphology and the pluripotency are well maintained; the use of the expensive heparitin sulfate is not required so as to substantially reduce the cost; and almost all of the hiPSCs maintaining cultures at the current stage can be met, and various small molecule compounds are added to perform induction reprogramming culture (removal of TGFB1 and DM), such the culture medium is suitable for extensive researches of various basic scientific researches and clinical scientific research experiments.

An oxygen absorber is provided which is obtainable by mixing 10 to 60 parts by weight of a particulate thermoplastic resin having a softening point of 90 to 125 DEG C. and a particle diameter of 1 to 500 mu m with 100 parts by weight of an oxygen absorbing composition containing an ascorbic acid compound, water and an activated carbon. The oxygen absorber can inhibit overheat to prevent spontaneous ignition. Further, the activated carbon has a particle diameter of 0.1 to 2 mm and the ascorbic acid compound includes L-ascorbic acid, sodium L-ascorbate, calcium L-ascorbate and sodium D-iso-ascorbate.

The invention discloses a freshwater fish feed. The freshwater fish feed is prepared from the following raw materials in parts by weight: 10-20 parts of bean pulp, 20-30 parts of corns, 20-30 parts of broken rice, 10-20 parts of rice bran, 5-8 parts of soybean oil, 0.5-1 part of compound amino acid, 1-3 parts of complex vitamin, 1-3 parts of mineral substances, 1-3 parts of calcium L-ascorbate and 3-5 parts of Chinese herb extract. The freshwater fish feed is capable of effectively solving the problem that a compound feed is low in protein utilization rate and pollutes the environment in the freshwater fish farming process, and therefore, the industrial profit is increased.

The invention provides a method for detecting allergens in cosmetics. The method comprises the steps of preparing a mixed standard solution of 56 allergen substances by a mixed solution of methanol and a 2g/L ascorbic acid aqueous solution, wherein single standard solutions are independently prepared for seven groups of isomers such as eugenol, isoeugenol and the like; carrying out mass spectrometry analysis in modes of positive-negative ion primary mass spectrometry full scanning and data-dependent secondary mass spectrometry scanning of a high-resolution mass spectrum of an electrostatic field orbitrap; collecting an accurate mass number of primary parent ions, retention time and secondary fragment ion information to establish a high-resolution mass spectrum database; carrying out mass spectrometry analysis on a to-be-detected sample by adopting extraction of the mixed solution of the methanol and the 2g 2g/L ascorbic acid aqueous solution; by utilizing the database, establishing screening and quantitative analysis methods by using Tracerfinder software; and detecting the allergen substances in the sample. The method is rapid, high in sensitivity and high in specificity.

The invention provides a feed capable of alleviating the heat stress of beef cattle. The feed is composed of the following raw materials in percentage by weight: 30-40% of corn, 30-40% of wheat bran, 16% of bean pulp, 5% of callicarpa nudiflora, 2-5% of mung beans, 2-4% of chinese mesona herb, 0.5% of potassium chloride, 0.5% of table salt, 1% of sodium bicarbonate, 0.5% of magnesium oxide and 1.5% of sodium L-ascorbate. The feed provided by the invention is capable of improving the heat stress of beef cattle in summer, solving the production problem of beef cattle in summer, and reducing the breeding risk.

The invention relates to a quality-improving and harm-reducing modifier for tobacco modulation and a use method thereof. The modifier contains salicylic acid with the concentration of 0.5-2 g/L, potassium citrate with the concentration of 3-6 g/L, chitosan with the concentration of 5-15 g/L and calcium ascorbate with the concentration of 0.1-0.5 g/L according to the mass concentration. By means ofthe modifier, the adversity oxidative stress in the modulation process can be relieved, nitrate is restrained from being converted into nitrite, the content of TSNAs in cured tobacco leaves is reduced, the enzymatic browning reaction intensity is restrained, meanwhile potassium ions in the tobacco leaves are compensated for, and the sucking quality and safety of the cured tobacco leaves are improved.

The invention relates to a method for quickly measuring the trace antimony in steel, using the hydride generator-plasma atomic emission spectrometer and solution containing NaBH 4 as reducer, the mixed liquor containing thiourea, ascorbic acid and potassium iodide as masking inhibitor and alkali or alkali salt in the reducer as NaBH4 restraint decomposer. Preferably the masking inhibitor is the mixture of 8.0-13.0g/L NaBH 4, 4.0-8.0g/L NaOH, 4.0-8.0g/Lthiourea, 4.0-8.0g/Lascorbic acid and 2.0-5.5g/L KI and the analytical line of the antimony is 217.581(154)nm. The invention reduces the testing time and increases the measurement sensitivity and reduces the analysis detection limit.

The invention relates to a method for increasing attaching amount and conductivity of graphene on a cotton fabric, and belongs to the technical field of textile printing and dyeing. The method adoptsthe technology with the following steps of (1) adding a certain amount of cotton fabric anionic modifier into water, adjusting the pH (potential of hydrogen) value to 12.5 by sodium hydroxide, addinginto the to-be-treated cotton fabric, controlling the bath ratio to 1:20, and the mass ratio of cotton fabric to anionic modifier to 10:1, treating for 15min at the temperature of 90 DEG C, repeatedlywashing by water until the property of the washing solution is neutral, and drying the fabric; (2) after drying, treating the cotton fabric by a sodium periodate solution with concentration of 4 to 8g/L for 6h at the temperature of 25 DEG C under the light shading condition, controlling the bath ratio to 1:30, fully washing by water to remove the residual matter, and drying the cotton fabric; (3)impregnating the cotton fabric into a graphene oxide solution with concentration of 0.5 to 2mg/ml for 2h, controlling the bath ratio to 1:20, impregnating the cotton fabric into a water solution containing 10 to 15g/L ascorbic acid and 1ml/L ammonia water, controlling the bath ratio to 1:20, treating for 30min at the temperature of 95 DEG C, fetching out, washing by water, and drying. The methodhas the advantages that the cotton fabric is modified by anions, and properly oxidized, so that the attaching amount of graphene on the cotton fabric is increased; the conductive property is further improved.

The invention relates to a preparation method for L-ascorbic acid-2-monophosphate ester magnesium. The method which adopts no organic solvent during the preparation process does not cause environmental pollution with simple after treatment and high purity of the prepared L-ascorbic acid-2-monophosphate ester magnesium. The method is characterized in that: water, L-ascorbic acid and calcium chloride are added into a reaction kettle with a temperature between -5 DEG C and 10 DEG C for agitation, then 20-60 percent of sodium hydroxide or calcium hydroxide aqueous solution is added slowly into the reaction kettle, a PH value is regulated to 6-9.5, and sodium trimetaphosphate with a dosage of 0.8-1.5 folds of vc is added into the reaction kettle with the temperature being kept between 15 DEG C and 35 DEG C; the reaction products are washed by water, the PH value is regulated to 0.5-3.0 through hydrochloric acid, sulphuric acid or oxalic acid, then filtration is carried out, and filtrate liquor passes through weak anion resin and is eluted in turn by 0.02m hydrochloric acid and 0.2-0.8m hydrochloric acid; magnesia is used to regulate the PH value of an eluted part which is filtrated to remove deposit, filtrate liquor is decompressed and condensed to 1/5 of the original volume at a temperature of 40 DEG C, ethanol with a volume being 2-4 folds of that of the condensed filtrate liquor and a content of 95 percent is added, and then crushing and drying is carried out after crystallization, centrifugal collection and washing.

The invention relates to and provides a compound feed for juvenile mandarin fishes. The feed comprises the following components in parts by weight: 58-63 parts of super steam fish meal, 13-22 parts ofhigh-gluten flour, 3-10 parts of fermented soybean meal, 3.2-7.6 parts of hermetia illucens, 1-3 parts of blend oil consisting of frozen fish oil and corn oil, 1-3 parts of spirulina, 1-2 parts of minerals, 0.5-1.5 parts of calcium dihydrogen phosphate, 0.1-0.5 part of choline chloride, 0.13-0.35 part of taurine, 0.2-0.5 part of multi-vitamin and 0.1-0.3 part of L-ascorbate-2-phosphate, wherein the mass ratio of the frozen fish oil to the corn oil in the bend oil is 1:1. The compound feed for juvenile mandarin fishes of the invention has rich nutrients and high feed conversion rate, is easy to be digested and absorbed by fishes, and effectively promotes the healthy and fast growth of the juvenile mandarin fishes.

The invention relates to feed and a preparation method thereof, in particular to a puffing feed for preventing whitening of black carp body color and a preparation method of the feed. The puffing feed comprises the following components by weight: 7-15% of fish meal, 15-30% of peeled bean pulp, 10-20% of rapeseed dreg, 1-5% of squid paste, 6-12% of meat and bone meal, 3-6% of beer yeast, 2-5% of pine needle meal, 3-7% of robinia pseudoacacia leave powder, 2-6% of seaweed meal, 0.1-0.2% of L-ascorbate-2-phosphate, 0.15-0.65% of choline chloride, 3-7% of corn protein powder, 15-25% of flour, 1-2% of soya-bean oil, 1-2% of monocalcium phosphate and 1-2% of fish pre-mix compound. The preparation method of the feed comprises the following steps: 1) weighting the components; 2) mixing uniformly and crushing; 3) introducing steam into a conditioner for conditioning, and carrying out pelletizing in an extrusion puffing pelletizer; and 4) drying. The feed has the advantages of being complete innutrition, and capable of being digested and absorbed easily, promoting deposition of fish organism pigments, and preventing body color change; and the puffing feed is good in stability in water, andhigh in digesting efficiency, and reduces the feed waste.

Provided is a patch containing fentanyl or a derivative thereof which causes no lowering in the stickiness to the skin, has a high drug-stability, can inhibit the time-dependent deposition of fentanyl crystals, yet little irritates the skin and shows an extremely high skin permeability of fentanyl. A patch containing fentanyl or a derivative thereof consisting of a support, a pressure-sensitive adhesive layer containing fentanyl or a derivative thereof and a release liner, wherein the pressure-sensitive adhesive layer containing fentanyl or a derivative thereof contains 0.01 to 0.5% by mass of an ascorbate such as an L-ascorbate palmitate. A patch containing fentanyl or a derivative thereof further contains a hindered phenol-type antioxidant in an amount sufficient for the inhibition of the deposition of fentanyl or a salt of the same.

The invention discloses original orange-flavor buccal tablets prepared from sodium L-ascorbate, and a preparation method of the buccal tablets. The buccal tablets are prepared from the following raw materials in parts by weight: 500-1500 parts of fresh oranges, 100-300 parts of sodium L-ascorbate, 150-350 parts of isomaltulose, 100-250 parts of Arabic gum, 40-150 parts of honey and 0.5-2 parts of magnesium stearate. The buccal tablets maintain the original taste and flavor of the oranges and enhance the function of the sodium L-ascorbate, thus being a good vitamin supplement and a good health product; the buccal tablets are unique in production technology, good in product molding, simple in technology and easy to operate, and are suitable for industrial production.

The present invention relates to compound feed for adult fish of carassius auratus, belonging to the technical field of aquaculture. The compound feed comprises the following ingredients by weight: 7-14% of domestic fish meal, 6-9% of corn gluten meal, 22-28% of soybean meal, 12-16% of cottonseed meal, 10-13% of rapeseed meal, 7-9% of rice bran, 5.83-9.63% of wheat middling, 4-6% of Saccharomyces cerevisiae, 4.5-5.3% of soybean oil, 0.5-1.0% of soy lecithin, 0.7-1.5% of calcium dihydrogen phosphate, 0.05-0.1% of L-ascorbate-2-monophosphate, 3-4% of zeolite powder, 0.2-0.3% of choline chloride, 0.2-0.4% of salt, 0.01-0.03% of ethoxyquin, 0.5-1.0% of mineral premix, and 0.3-0.8% of vitamin premix. According to the present invention, habits, nutritional needs and digestion characteristics of carassius auratus are fully taken into account, and the compound feed is reasonable in combination by selecting raw materials easily digested and absorbed by carassius auratus, and has good palatability, a high feed conversion ratio, little pollution to water bodies, stable sources of raw materials, and low cost, suitable for large-scale adult fish farming of carassius auratus.

The invention discloses a pharmaceutical composition containing sodium vitamin C and a preparation method thereof. The pharmaceutical composition contains the following components in parts by weight: 75-95 parts of sodium vitamin C, 5-10 parts of potassium chloride, pharmaceutically acceptable 0‑10 parts of accessories. The present invention overcomes the disadvantages of existing vitamin C such as high acidity and easy oxidation, and solves the clinical problem that potassium chloride produced by the combination of vitamin C and potassium chloride accelerates the oxidation of vitamin C, etc., so that the product has better stability and more definite curative effect It is suitable for patients who lack vitamin C and potassium in clinic. It can be used to treat scurvy, infectious diseases, purpura and other diseases with muscle weakness, paralysis, arrhythmia or renal dysfunction. It can also be used for malignant tumors, heart and brain. Prevention and adjuvant treatment of vascular diseases, infectious diseases, autoimmune diseases and other major difficult diseases.

The invention discloses a controlled atmosphere combined preservation method capable of controlling condensate water on surfaces of fruit vegetables and belongs to the technical field of preservation of fruits and vegetables. The water evaporation function and the breathing function of the fruit vegetables are inhibited with a coating-combined staged controlled atmosphere preservation technology. A coating substance composition substrate takes a composite coating agent prepared from chitosan, gelatin and glyceryl monostearate in the ratio being 1:1:1 as a coating material and has the antibacterial activity and the oxidation resistance enhanced by adding 2.5 g/L sorbic acid and 0.25 g/L ascorbic acid. By means of a designed temperature gradient controlled atmosphere storage, the condition of non-uniform temperature in the storage can be relieved, and temperature difference between the storage temperature and the temperature of materials is reduced. By means of the staged controlled atmosphere preservation technology, the controlled atmosphere conditions are changed according to different breathing degrees at the earlier and later stages, so that the vegetable breathing function is better inhibited, and production of the condensate water is reduced. By means of the combined preservation technology, production of 90% of surface condensate water is reduced, and the storage period of the fruit vegetables is prolonged to 40 days or longer.

The invention relates to the technical field of aquaculture, in particular to a general type shell solidifying additive for shrimps and crabs, and a preparation method of the general type shell solidifying additive. The general type shell solidifying additive for shrimps and crabs provided by the invention is prepared from the following components in percentage by mass of 25%-30% of shrimp shell powder, 15%-20% of cuttlefish visceral meal, 25%-30% of calcium dihydrogen phosphate, 0.5%-1% of ferrous sulfate, 1%-2% of cupric glycinate, 2%-3% of zinc methionine, 1%-2% of manganese glycine, 1%-2%of astaxanthin, 3%-5% of cholesterol, 4%-6% of chitosan, 5%-8% of L-ascorbate-2-monophosphate having content being 35%, 0.5%-1% of vitamin D3 and 1%-2% of vitamin E. Through the adoption of the general type shell solidifying additive for shrimps and crabs disclosed by the invention, the problems of soft shells, failing of exuviation, shell solidifying and the like in the breeding course of the shrimps and the crabs can be solved, the survival rate of the shrimps and the crabs can be effectively increased, the immunity and the resistance of the shrimps and the crabs can be improved, and growthcan be promoted.