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Kit for detecting 1, 5-anhydro-D-ghlcitol in human blood

A sorbitan and reagent kit technology, applied in the field of medical testing, can solve problems such as endogenous interference and poor stability, and achieve the effects of improving specificity and accuracy, high sensitivity, and simple operation

Active Publication Date: 2015-04-01
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the enzymatic kits currently on the market have poor stability and are susceptible to endogenous interference such as glucose, bilirubin, ascorbic acid, and blood lipids.

Method used

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  • Kit for detecting 1, 5-anhydro-D-ghlcitol in human blood
  • Kit for detecting 1, 5-anhydro-D-ghlcitol in human blood
  • Kit for detecting 1, 5-anhydro-D-ghlcitol in human blood

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Preparation of 1,5-sorbitol detection reagent.

[0039] 1. Prepare according to the formula in Table 1 below

[0040] Table 1 1,5-sorbitol detection reagent formula

[0041]

[0042]

[0043] 2. Preparation of 1,5-sorbitol calibrator and quality control product: Weigh the required amount of pure product (purchased by Sigma) and dissolve it in purified water, add 0.1% sodium azide as preservative, and store in a closed place for future use.

[0044] 3. Set the following parameters on the automatic biochemical analyzer: reaction 10min, reaction temperature 37°C, main wavelength 546nm, subwavelength 700nm, sample: reagent 1: reagent 2 = 6ul: 180ul: 60ul, reaction type: endpoint method.

Embodiment 2

[0046] 1,5-Sorbitol Detection Kit Performance Verification

[0047] 1. Sensitivity and precision test

[0048] Use pure 1,5AG with a concentration of 152.3uM as a standard for testing, where S1 is water, S2

[0049] The point is 1,5AG at 152.3uM, see Table 2 for details:

[0050] Table 2

[0051] Absorbance (S1) Absorbance (S2) Recipe 1 0.0122 0.0648 Recipe 2 0.0118 0.0639 Recipe 3 0.0128 0.0650

[0052] Table 3 precision test

[0053] Intra-assay precision Inter-assay precision Recipe 1 2.5％ 3.8％ Recipe 2 3.1％ 4.2％ Recipe 3 3.6％ 4.9％

[0054] 2. See Table 4 for anti-interference performance

[0055] Table 4

[0056]

[0057]

[0058] It can be seen from the above table that the following interfering substances have no obvious interference on this kit: vitamin C less than 20mg / dL, conjugated bilirubin less than 30mg / L, unconjugated bilirubin less than 20mg / L, glucose less than 30mmol / L...

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Abstract

The invention discloses a kit for detecting 1, 5-anhydro-D-ghlcitol in human blood. R1 comprises components as follows: 20-200 mM of a buffer solution, 1-10 mM of 4-aminoantipyrine, 0.2-2 mM of ATP (adenosine triphosphate), 1-10 mM of PEP (phosphoenolpyruvate), 1-50 KU / L of ATP-HK, 1-50 KU / L of pyruvate kinase, 1-50 KU / L of ascorbic acid oxidase, 0.05-10 mM of sodium vanadate, 0.01%-5% of a surface active agent, 0.1%-10% of a stabilizer and 0.01%-1% of a preservative; and R2 comprises components as follows: 20-200 mM of a buffer solution, 1-10 mM of TOOS (N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline,sodium salt), 1-100 KU / L of POD (peroxidase), 10-200 KU / L of PROD (pyranose oxidase), 0.01%-5% of a surface active agent, 0.1%-10% of a stabilizer and 0.01%-1% of a preservative. The kit has the advantages that endogenous interference of glucose, bilirubin, ascorbic acid, blood lipid and the like is effectively prevented and the stability is good.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a kit for detecting 1,5-sorbitol in human blood. Background technique [0002] Diabetes is a common clinical disease and a frequently-occurring disease. It is caused by insufficient insulin secretion. There is a significant negative correlation. According to data, none of the patients with plasma glucose (GLU)>8.3mmol / L was accompanied by a high level of plasma 1,5-sorbitol (1,5-AG). [0003] 1,5-anhydroglucitol (1,5-AG) has a significant negative correlation with existing diabetes indicators, and the degree of reduction of 1,5-anhydroglucitol in blood is significantly related to the severity of diabetes. The most significant difference between 1,5-anhydroglucitol (1,5-AG) and other indicators is that it reflects the different cycle of diabetes mellitus. The average status of blood sugar in a month, glycosylated hemoglobin (HbAlc) reflects the average blood sugar le...

Claims

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Application Information

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IPC IPC(8): G01N33/66G01N21/78
CPCG01N21/78
Inventor 邹炳德邹继华汪屹贾江花
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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