4 results about "Fluorescence biosensor" patented technology

This invention discloses a method for detecting organophosphorus pesticides using a self-cascaded Pt@Fe-CDs nanozyme, belonging to the field of chemical analysis and detection technology. This method synthesizes Pt@Fe-CDs nanozymes using iron-doped carbon dots (Fe-CDs) as a reducing agent and template. Pt@Fe-CDs simultaneously possess ascorbic acid oxidase-like and peroxidase-like activities. Under the catalysis of alkaline phosphatase, they hydrolyze L-ascorbic acid-2-phosphate trisodium salt to AA. Based on the fact that organophosphorus pesticides can inhibit ALP activity and hinder AA2P dephosphorylation, leading to a reduction in AA and thus causing changes in fluorescence and colorimetric signals, a simple and sensitive dual-signal fluorescence / colorimetric sensing platform for organophosphorus pesticides is constructed, with a detection limit of 0.28-0.53 ng / mL. The colorimetric and fluorescence biosensors established in this invention exhibit excellent performance in terms of sensitivity, specificity, and accuracy in actual samples.

This invention proposes a capsaicin nucleic acid aptamer and its ratiometric fluorescent biosensor application. A capsaicin-specific nucleic acid aptamer with nanomolar affinity for capsaicin was obtained using the RNase-SELEX screening strategy. Furthermore, a ratiometric fluorescent biosensor was constructed using the pruned aptamer. A dual-signal response is achieved through competitive binding / conformational regulation of the aptamer by capsaicin and the nucleic acid dye SYBR Green II. Quantitative detection of capsaicin is achieved through a linear relationship between the fluorescence ratio and capsaicin concentration, exhibiting advantages such as high sensitivity and strong anti-interference capability.

Disclosed are leave-one-out GFP (LOO_GFP) biosensors immobilized in Ubx (Drosphila protein Ultrabithorax) that form immobilized and stabilized fluorescent protein biosensor systems (LOO_GFP-Ubx systems). These LOO_GFP-Ubx systems have an enhanced fluorescent signaling to noise ratio. Also disclosed are methods of making LOO_GFP-Ubx systems and methods of using such systems to detect analyte. In preferred embodiments, the LOO_GFP-Ubx system has the eighth β strand or eleventh f strand of OPT GFP left out.