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124 results about "Fluorescence biosensor" patented technology
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Long wave fluorophore sensor compounds and other fluorescent sensor compounds in polymers
InactiveUS6766183B2Improve sensor sensitivityReduction in the tissue autofluorescence backgroundMicrobiological testing/measurementChemiluminescene/bioluminescenceConcentrations glucoseFluorophore
Owner:MEDTRONIC MIMIMED INC +1
Polymers functionalized with fluorescent boronate motifs and methods for making them
InactiveUS6927246B2Enhance swellabilityGood biocompatibilityGroup 3/13 element organic compoundsBiological testingAnalyteConcentrations glucose
Improved polymer matrices which incorporate fluorescent biosensor molecules as well as methods of making and using these polymer matrices are described. Such matrices can be used in fluorescent biosensors and biosensor systems, including those which are used in the detection of polyhydroxylated analytes such as glucose. The properties of the polymer matrices of the invention renders biosensors utilizing such matrices particularly well-suited for detecting and measuring in-vivo glucose concentrations.
Owner:MEDTRONIC MIMIMED INC
Analyte sensing via acridine-based boronate biosensors
InactiveUS7045361B2Improve sensor sensitivityIncrease overall biocompatibility and functioningMicrobiological testing/measurementChemiluminescene/bioluminescenceConcentrations glucoseFluorophore
Fluorescent biosensor molecules, fluorescent biosensors and systems, as well as methods of making and using these biosensor molecules and systems are described. These biosensor molecules address the problem of obtaining fluorescence emission at wavelengths greater than about 500 nm. Biosensor molecules generally include an (1) an acridine-based fluorophore, (2) a linker moiety and (3) a boronate substrate recognition / binding moiety, which binds polyhydroxylate analytes, such as glucose. These biosensor molecules further include a “switch” element that is drawn from the electronic interactions among these submolecular components. This fluorescent switch is generally “off” in the absence of bound polyhydroxylate analyte and is generally “on” in the presence of bound polyhydroxylate analyte. Thus, the reversible binding of a polyhydroxylate analyte essentially turns the fluorescent switch “on” and “off”. This property of the biosensor molecules, as well as their ability to emit fluorescent light at greater than about 500 nm, renders these biosensor molecules particularly well-suited for detecting and measuring in-vivo glucose concentrations.
Owner:MEDTRONIC MIMIMED INC
Long wave fluorophore sensor compounds and other fluorescent sensor compounds in polymers
InactiveUS20020193672A1Improve accuracyEliminate errorsMicrobiological testing/measurementChemiluminescene/bioluminescenceConcentrations glucoseFluorophore
Fluorescent biosensor molecules, fluorescent biosensors and systems, as well as methods of making and using these biosensor molecules and systems are described. Embodiments of these biosensor molecules exhibit fluorescence emission at wavelengths greater than about 650 nm. Typical biosensor molecules include a fluorophore that includes an iminium ion, a linker moiety that includes a group that is an anilinic type of relationship to the fluorophore and a boronate substrate recognition / binding moiety, which binds glucose. The fluorescence molecules modulated by the presence or absence of polyhydroxylated analytes such as glucose. This property of these molecules of the invention, as well as their ability to emit fluorescent light at greater than about 650 nm, renders these biosensor molecules particularly well-suited for detecting and measuring in-vivo glucose concentrations.
Owner:MEDTRONIC MIMIMED INC +1
Optical fiber biological sensor
InactiveCN101082584AHigh sensitivityGood choiceBiological testingFluorescence/phosphorescenceFiberData acquisition
The invention discloses a fibre-optical fluorescent biological sensor, which comprises the following parts: optical exciting path, optical detecting path and fluorescent receiving optical path, wherein three optical paths are connected on three branches of Y-shaped optical fiber; the optical exciting source utilizes the coupling light to couple on one branch of Y-shaped optical fiber on the optical exciting path; another branch of Y-shaped optical fiber is used to fix biological identifier, which is connected with the carrier or sample pond; the third branch of Y-shaped optical fiber is connected with the data gathering system, which is connected with signal disposing and display system further. The invention possesses rapid response, high sensitivity, good selectivity, light weight, small bulk and good friend biological property, which is fit for all fluorescent markers in the biological molecular identifying, medical clinical diagnosis, high-flux drug sieving, environment monitoring, and biochemical agent detecting domain.
Owner:DALIAN MARITIME UNIVERSITY
Fluorescence biosensor, and preparation method and purpose thereof
ActiveCN106483110AEasy to synthesizeReduce energy consumptionFluorescence/phosphorescenceVitamin CPhosphate
The invention relates to a fluorescence biosensor, and a preparation method and a purpose thereof. On the basis of a two-dimensional manganese dioxide nanometer sheet as a fluorescence sensing platform, morpholineethanesulfonic acid is used for reducing potassium permanganate for synthesizing the manganese dioxide nanometer sheets; the manganese dioxide nanometer sheets adsorb FAM-ssDNA and quench FAM fluorescence; under the effect of alkaline phosphatase, vitamin C magnesium L-ascorbate-2-phosphate is hydrolyzed to generate ascorbic acid; the two-dimensional manganese dioxide nanometer sheets are reduced into manganese ions by the ascorbic acid; FAM-ssDNA is released; the fluorescence is recovered; the fluorescence sensor using the manganese dioxide nanometer sheets and the alkaline phosphatase to regulate the fluorescence intensity is built and is used for detecting the activity of the alkaline phosphatase. Compared with the prior art, the fluorescence biosensor has the characteristics of simplicity, high speed, low background signal, high sensitivity, low detection limit and good selectivity by using the change of the two-dimensional manganese dioxide nanometer sheets on the fluorescence signal, i.e., the off-on mode for detecting the alkaline phosphatase.
Owner:ANHUI NORMAL UNIV
Analyte sensing via acridine-based boronate biosensors
InactiveUS20050191761A1Good biocompatibilityFunction increaseMicrobiological testing/measurementChemiluminescene/bioluminescenceConcentrations glucoseFluorophore
Fluorescent biosensor molecules, fluorescent biosensors and systems, as well as methods of making and using these biosensor molecules and systems are described. These biosensor molecules address the problem of obtaining fluorescence emission at wavelengths greater than about 500 nm. Biosensor molecules generally include an (1) an acridine-based fluorophore, (2) a linker moiety and (3) a boronate substrate recognition / binding moiety, which binds polyhydroxylate analytes, such as glucose. These biosensor molecules further include a “switch” element that is drawn from the electronic interactions among these submolecular components. This fluorescent switch is generally “off” in the absence of bound polyhydroxylate analyte and is generally “on” in the presence of bound polyhydroxylate analyte. Thus, the reversible binding of a polyhydroxylate analyte essentially turns the fluorescent switch “on” and “off”. This property of the biosensor molecules, as well as their ability to emit fluorescent light at greater than about 500 nm, renders these biosensor molecules particularly well-suited for detecting and measuring in-vivo glucose concentrations.
Owner:MEDTRONIC MIMIMED INC
Fluorescence biosensor for detecting UDG (Uracil-DNA Glycosylase) and preparation method thereof
ActiveCN109444105ARealize highly sensitive detectionLow detection limitFluorescence/phosphorescenceEnergy transferResonance
The invention relates to the technical field of a biosensor, and particularly relates to a fluorescence biosensor for detecting UDG (Uracil-DNA Glycosylase) on the basis of a polymerase-assisted feedback rolling circle amplification and endonuclease amplification fluorescence method. The invention aims to solve problems of both low specificity and low sensitivity of a method for detecting UDG in the prior art. The biosensor for detecting the UDG on the basis of a feedback rolling circle amplification technology achieves a rolling circle amplification effect on matching of phi29 polymerase andendonuclease IV, implements fluorescence resonance energy transfer of a fluorophore and a Quenching group and performs a homogeneous reaction on mixed liquid. A preparation method comprises: constructing a circular template and a composite probe; feeding back a rolling circle amplification signal and carrying out fluorescence detection. Specific hydrolysis of the UDG on a basic group U is utilized, and by utilizing such specific reaction, the UDG can be accurately measured and meanwhile, interference can also be avoided; by utilizing endonuclease IV circle amplification, a signal amplificationeffect is achieved.
Owner:UNIV OF JINAN
Fluorescent biological sensor for detecting uracil-DNA glycosylase (UDG)
InactiveCN108088826AStrong specificityHigh sensitivityFluorescence/phosphorescenceSilver clusterUracil-DNA glycosylase
The invention provides a fluorescent biological sensor for detecting uracil-DNA glycosylase (UDG). The fluorescent biological sensor comprises an annular template, a hairpin probe, a fluorescent probe, dNTP, phi 29 DNA polymerase and endonuclease IV. The biological sensor disclosed by the invention has the advantages of good specificity, high flexibility, moderate reaction condition and quick reaction speed; by means of silver cluster fluorescent detection, the biological sensor has the advantages of convenience in operation, short detection period, easiness in carrying, low technological cost, suitability for an industrial inexpensive requirement; a preparation method has the advantages of simpleness, stable performance and good repeatability.
Owner:UNIV OF JINAN
Fluorescence biosensor
ActiveUS7541176B2Improve fluorescence sensitivityHigh sensitivityBioreactor/fermenter combinationsRaman/scattering spectroscopyPhotovoltaic detectorsIntegrator
Owner:STMICROELECTRONICS (RES & DEV) LTD
Fluorescence biosensor for detecting high-sensitivity copper ionss and detection method thereof
InactiveCN102608092AHigh sensitivityLow detection limitFluorescence/phosphorescenceQuantum dotCopper
The invention relates to a fluorescence biosensor for detecting high-sensitivity copper ions and a detection method thereof, which relate to the biosensor and a method for detecting the copper ions in water by utilizing the biosensor. In order to solve the problems of low sensitivity, poor selectivity and the like in existing copper detection, the fluorescence biosensor for detecting the copper ions is made of a quantum dot, enzyme and an enzyme substrate. The detection method comprises the following steps of: adding the enzyme into quantum dot solution, adding the enzyme substrate, and measuring the change of fluorescence intensity of the quantum dot; and mixing the enzyme and the copper ions with different concentration, adding the mixture into the quantum dot solution, adding the enzyme substrate, measuring the change of fluorescence intensity of the quantum dot, drawing according to relative fluorescence intensity-copper ions, and calculating a detection limit of the copper ions. The process is simple, the price is low, the reaction condition is moderate, the operation is easy, and the reproducibility is good. The method is a very promising detection technology, and is suitable for trace detection of the copper ions in environment and even bio-system.
Owner:HARBIN INST OF TECH
Method for preparing acetylcholinesterase fluorescence biosensor
InactiveCN101581670AGood storage stabilityLow costMaterial analysis by electric/magnetic meansBiological testingOrganismOrganophosphorus pesticides
The invention relates to a method for preparing an acetylcholinesterase fluorescence biosensor. The invention solves the following problems: the existing enzyme biosensor is prone to being affected by environmental influence so that the enzyme losses activity thus resulting false- positive; the existing biosensor is not allowed for repeated use due to irreversible reactions, in which enzymes are expensive, so that the cost of the equipment is excessively high; the selectivity is low, etc. The method comprises the following steps: firstly, carrying out the extraction separation and purification on acetylcholinesterase in crucian carp brain; then, carrying out the immobilization on the acetylcholinesterase in the crucian carp brain by chitosan; preparing immobilized acetylcholinesterase membranes, namely, obtaining the acetylcholinesterase fluorescence biosensor. The acetylcholinesterase fluorescence biosensor prepared by the invention dispenses with neither external power connection nor reference electrodes, and has the advantages of small size, light weight, high response speed, electromagnetic interference resistance, corrosion resistance, high sensitivity, wide measurement bandwidth, convenient operation, low cost and the like, therefore, the invention is applicable to the rapid detection of organophosphorus pesticide in food.
Owner:王振宇
Bovine serum albumin-stabilized copper nano-cluster fluorescence biosensor and preparation method and application thereof
InactiveCN105699349AEasy to prepareMild reaction conditionsFluorescence/phosphorescenceQuantum yieldBiocompatibility Testing
The invention discloses a bovine serum albumin-stabilized copper nano-cluster fluorescence biosensor and a preparation method and application thereof. The preparation method mainly comprises the step of preparation of a bovine serum albumin-stabilized copper nano-cluster; the fluorescence biosensor is used for sensitivity detection on hydrogen peroxide. According to a preparation method of the bovine serum albumin-stabilized copper nano-cluster, the copper nano-cluster with the fluorescence property is prepared by taking bovine serum albumin as a stabilizing agent and taking a copper sulphate solution as a copper source. The preparation method of the fluorescence copper nano-cluster is simple and low in cost and does not need any reducing agents, the prepared fluorescence copper nano-cluster has the advantages of being low in toxicity, good in light stability, high in quantum yield, good in biocompatibility and the like, and high sensitivity determination on hydrogen peroxide can be achieved through the copper nano-cluster. The copper nano-cluster has the wide application prospect in the fields of food safety, biochemical analysis, cell imaging, clinical diagnosis and the like by serving as a fluorescence probe.
Owner:XINYANG NORMAL UNIVERSITY
Method for preparing fluorescence biosensor and application of fluorescence biosensor
ActiveCN105203515ADetection sensitivityDetection specificityFluorescence/phosphorescenceTrue positive rateThymine
The invention discloses a method for preparing a fluorescence biosensor and an application of the fluorescence biosensor. Under the light-emitting effect of copper nano particles with polythymine-carried single-stranded DNAs used as the template, the phenomenon that light induction electron transfer is generated between G-four-linked bodies and the copper nano particles, and thus emitted light of the copper nano particles is quenched is utilized, so that the copper nano particles based on the thymine-carried single-stranded DNAs used as the template and a light induction electron transfer system are prepared for establishing the fluorescence sensor. The principle that targets (HBV genes) and probes are combined to form the system of a compact structure, so that the distance between the G-four-linked bodies and the copper nano particles is zoomed out is adopted, and the sensitivity and specificity of the HBV genes are detected.
Owner:ANHUI NORMAL UNIV
miRNA detection fluorescent biological probe and detection method and application thereof
ActiveCN109913546ASimple stepsAvoid thermal cyclingMicrobiological testing/measurementDNA/RNA fragmentationBiological targetFluorescence biosensor
Owner:JIANGSU INST OF NUCLEAR MEDICINE
Preparation method of fluorescent biosensor of double-cycle and double quenching system based on functionalized graphene quantum dots
InactiveCN109593831AGood water solubilityEnhanced fluorescence emission intensityMicrobiological testing/measurementDissolutionQuenching
The invention relates to a preparation method of a fluorescent biosensor of a double-cycle and double quenching system based on functionalized graphene quantum dots. The method comprises the followingsteps: weighing raw materials including citric acid, histidine and pentaethylene hexamine at the molar ratio of 0.01:0.1:1 to 1:1:1, uniformly mixing, adding deionized water, then performing ultrasonic dissolution to obtain a reaction solution, placing the reaction solution in an oven, reacting for 0.5-10h at an oven temperature of 150-250 DEG C to directly obtain a crude product of pentaethylenehexamine-histidine graphene quantum dot PEHA-GQD-His after reaction, then obtaining an LDNA-capped pentaethylene hexamine-histidine graphene quantum dot PEHA-GQD-His solution, finally forming a G-quadruplex / DNase, and finally adding 0.5-100[mu]l of H2O2 with the molar concentration of 0.1-10[mu]M to obtain the fluorescent biosensor of the double-cycle and double quenching system.
Owner:JIANGNAN UNIV
Fluorescent biosensor based on high-gradient magnetic separation and quantum point as well as application thereof
ActiveCN108287236AAchieve specific separationRealize quantitative detectionFluorescence/phosphorescenceMagnetic beadSoft magnet
The invention provides a fluorescent biosensor based on high-gradient magnetic separation and a quantum point as well as application thereof. The fluorescent biosensor comprises a high-gradient magnetic separation device and a fluorescence detection system; the high-gradient magnetic separation device comprises an annular magnet array, a magnet bracket, a ring channel and an iron bead chain; the annular magnet array and the magnet bracket form an annular magnetic field generator; every two adjacent magnets are mutually repulsed and assembled; two capillary tubes are coaxially fixed and then are nested in the concentric circle of the annular magnet array; the inner capillary tube is filled with the iron bead chain made of a soft magnet material; the iron bead chain can be magnetized by thering magnetic field, so that nanometer magnetic beads are distributed in the ring channel uniformly and are used for capturing a target object. The fluorescent biosensor provided by the invention canbe used for quantitatively detecting microorganisms, is simple to operate, high in separation efficiency and high in sensitivity, and can realize quantitative detection on a small amount of target bacteria in food samples within 2 hours.
Owner:CHINA AGRI UNIV
Fluorescent material capable of visual detection of ammonia gas as well as preparation method and application of fluorescent material
ActiveCN104178133AQuick checkThe detection effect is immediateOrganic chemistryMaterial analysis by observing effect on chemical indicatorSolventFluorescence biosensor
The invention discloses a fluorescent material capable of visual detection of ammonia gas as well as a preparation method and an application of the fluorescent material, and belongs to the field of fluorescent biosensors. The fluorescent material is of a 1-(4'-carboxylic biphenyl)-2,5-diphenyl pyrrole (abbreviated as CP-TPP) film material, and is formed after the natural volatilization of a CP-TPP solution taken as a solvent which is dropwise smeared on a quartz plate. The fluorescent material which belongs to a solid film material is convenient to carry, has a specific response to the ammonia gas so as to rapidly detect the ammonia gas, and is high in sensitivity and selectivity. The preparation method is simple, convenient to operate and low in cost. The fluorescent material is simple to operate and obvious in effect during the detection of the ammonia gas, can be recycled and can be completely independent of a high precision instrument according to actual needs, thereby meeting the requirement of modern people on ammonia gas detection to a large extent.
Owner:BEIJING INSTITUTE OF TECHNOLOGYGY
Preparation method of chitosan 6-OH immobilized cyclodextrin derivative and application thereof in H2O2 detection fluorescent biosensor
InactiveCN103087328AImprove solubilityImprove responsivenessFluorescence/phosphorescenceSolubilityChemical reaction
The invention relates to a preparation method of a chitosan 6-OH immobilized cyclodextrin derivative, and an application thereof in a H2O2 detection fluorescent biosensor. The invention belongs to the field of high-molecular material preparation and modification. According to the invention, chitosan 2-NH2 is protected by using phthalic anhydride; through a click chemical reaction, cyclodextrin is immobilized at a 6-OH position; and the protection of chitosan 2-NH2 is removed by using hydrazine hydrate, such that the chitosan 6-OH immobilized cyclodextrin derivative with good solubility in water and part of organic solvents and with high immobilization rate is prepared. Fluorescent probe is immobilized in cyclodextrin hydrophobic hollow cavities of the chitosan 6-OH immobilized cyclodextrin derivative provided by the invention, 2-NH2 of chitosan pyranose ring is used for immobilizing catalase, and the material is used in a fluorescent biosensor used for detecting H2O2 content. The invention assists in realizing the application of chitosan 6-OH immobilized cyclodextrin derivative in the fluorescent biosensor in which enzyme catalysis is combined with fluorescence detection.
Owner:BEIJING INSTITUTE OF TECHNOLOGYGY
Fluorescence biosensor for vascular endothelial growth factor detection in breast cancer
InactiveCN104531880AImprove stabilityGood reproducibilityMicrobiological testing/measurementFluorescence/phosphorescenceA-DNAMolecular binding
The invention belongs to the field of sensors, and particularly relates to a fluorescence biosensor for vascular endothelial growth factor detection in breast cancer. The fluorescence biosensor comprises nitrogen carbide nanometer materials, silver ions and a DNA probe. The gene of the DNA probe is 5'-CCCCCCTGTGGGGGTGGACGGGCCGGGTAGACCCCCC-3'. The silver ions and the DNA probe form a hairpin structure. Under the induction of a target molecule vascular endothelial growth factor, the NDA probe and target molecules are combined to form G-quadruplex structure, the silver ions are released, and fluorescence signals of the nitrogen carbide nanometer materials are quenched. When the fluorescence biosensor is used for early diagnosis of breast cancer, the detection limit of the fluorescence biosensor to the vascular endothelial growth factor is 3.5 pmol / L. When the fluorescence biosensor is used for clinical application, detection is more is more accurate, more sensitive, more economical, easier, more convenient and noninvasive, and the fluorescence biosensor is of great significance.
Owner:FUJIAN MEDICAL UNIV
Fluorescent reagent for trace serum albumin, and preparation and application of fluorescent reagent
InactiveCN104194771AEnhanced signalHigh sensitivityOrganic chemistryFluorescence/phosphorescencePhenylboronic acidBromine
The invention discloses a fluorescent reagent for detecting trace serum albumin, and a preparation and application of the fluorescent reagent, and belongs to the field of fluorescent bio-sensors. The fluorescent reagent is prepared from DP-TPPNa and a good solvent. The preparation method comprises the following steps: dissolving 3,4-dibromo-1,2,5-tri((methoxycarbonyl)phenyl) pyrrole in the good solvent, adding phenylboronic acid, a catalyst and basic salt, and reacting to obtain DP-TPP; dissolving the DP-TPP in a solvent, adding alkali, and reacting to obtain DP-TPPNa; and dissolving the DP-TPPNa in the good solvent to obtain the fluorescent reagent. The fluorescent reagent is capable of quantitatively detecting the serum albumin in serum, is simple in preparation method, convenient to operate and quick in response, has high sensitivity and high selectivity, is capable of producing an excellent visible detection signal, can completely get rid of the dependence on high-precision instruments according to actual requirements, and can meet the requirement of people on detecting the serum albumin in serum today.
Owner:BEIJING INSTITUTE OF TECHNOLOGYGY
Fluorescent reagent for detecting trace gamma-globulin, as well as preparation method and application thereof
InactiveCN105778894AFluorescent signal enhancementHigh sensitivityOrganic chemistryFluorescence/phosphorescencePotassium hexafluorophosphateSolvent
The invention discloses a fluorescent reagent for detecting trace gamma-globulin, as well as a preparation method and application thereof, and belongs to the field of fluorescent bio-sensors. The fluorescent reagent is prepared from TABD-Py-PF6 and a good solvent. The preparation method comprises the following steps: dissolving (1Z,3Z)-1,4-di(4-methoxycarbonyl) phenyl-1,4-dibromo-1,3-butadiene in the good solvent, adding 4-pyridine phenylboronic acid, a catalyst and a basic salt, and reacting to obtain TABD-Py; dissolving the TABD-Py in a solvent, adding iodomethane to synthesize iodate, adding potassium hexafluorophosphate, and reacting to obtain TABD-Py-PF6; dissolving the TABD-Py-PF6 in the good solvent to obtain the fluorescent reagent. The fluorescent reagent does not need participation of metal ions, has a specific lighting-type fluorescent response on gamma-globulin, has high and quick sensitivity on gamma-globulin detection, and has high selectivity. The fluorescent reagent has an excellent visible detection signal, can be completely separated from high-precision instruments according to actual requirement, and can meet current requirement for detecting the gamma-globulin in serum to a great extent.
Owner:BEIJING INSTITUTE OF TECHNOLOGYGY
Fluorescent biosensor for detecting kanamycin and preparation method and application thereof
ActiveCN109540860ARealize highly sensitive detectionAchieve signal amplificationFluorescence/phosphorescenceKanamycinFluorophore
Owner:UNIV OF JINAN
Time resolution fluorescent biosensor based on phosphorescent light emitting technology and application thereof
ActiveCN102866135ARealize quantitative detectionQuick QualificationFluorescence/phosphorescenceGreen-lightOpto electronic
The invention provides a time resolution fluorescent biosensor based on a phosphorescent light emitting technology and an application thereof. The time resolution fluorescent biosensor based on the phosphorescent light emitting technology comprises an exciting light path module, a photoelectric signal receiving and converting module, a control system module, an amplifying and shaping circuit, a coding interface circuit and a data cache module. The exciting light path module comprises a main detection exciting light module and an auxiliary optical scanning module. The photoelectric signal receiving and converting module comprises a main detection photoelectric conversion module and an auxiliary scanning photoelectric conversion module. According to the invention, a phosphorescent light emitting material (platinum / palladium porphyrin) is used as a biological marker, and the result is represented in form of an infrared optical signal under green light irradiation and can be interpreted by an instrument so as to quantitively detect a target object to be detected. According to the invention, a chromatographic test strip is divided into a sandwich method mode, a competition law mode, an indirect method mode and a capturing method mode to qualitatively and quantitively detect and analyze different objects to be detected in a sample quickly and sensitively according to different immunoreactions of the objects to be detected.
Owner:武汉睿奇生物工程有限公司
Fluorescent biosensor for detecting triphosadenine and preparation method thereof
ActiveCN109470673ARealize highly sensitive detectionLow detection limitFluorescence/phosphorescenceExonuclease IExonuclease III
The invention relates to the technical field of biosensors, in particular to a fluorescent biosensor for detecting triphosadenine based on rolling circle amplification and incision enzyme feedback amplification. The biosensor comprises two aptamer DNA sequences, a linear padlock probe, a connecting probe, an AP probe, a T4 DNA ligase buffer solution, exonuclease I, exonuclease III, a PBS buffer solution, dNTP, phi29 DNA polymerase and endonuclease IV. A preparation method of the fluorescent biosensor comprises the following steps: (1) constructing an annular template to prepare a compound probe; and (2) combining a compound probe with an incision enzyme and a target object to achieve signal amplification. The probe can be used for achieving detection of high specificity and ultra-sensitivity. The fluorescent biosensor is mild in reaction, fast to detect and good in repeatability.
Owner:UNIV OF JINAN
Nano fluorescence biosensor as well as preparation method and application thereof
The invention provides a new nano fluorescence biosensor with a biological release control function as well as a preparation method and application thereof, wherein gold nano particles are used as an orifice cap of a gold nano cage. The sensor has the following characteristics: a DNA hybridization technology is adopted, and the gold nano particles are assembled on the surface of the gold nano cage to form an orifice cap switch to realize blocking of matters in the gold nano cage. The nano biosensor provided by the invention has the characteristics of simple structure, sensitive signal, strong controllability and the like. By adopting the nano fluorescence biosensor provided by the invention, high-sensitivity detection of ATP can be realized in a range of 1.0*10<-9>-1.0*10<-7>M; and the nano fluorescence biosensor has the advantages of sensitivity, economy, easiness in operation and the like, gains relatively great application potential and broad application prospects in the fields such as biomedicine and life science, and provides a new way and method for early diagnosis and treatment of serious diseases and particularly for the study and application of tumor-targeted drugs and photo-thermal therapy based on the nano carrier release control technology.
Owner:QINGDAO UNIV OF SCI & TECH
Biosensor for detecting ampicillin, and preparation method and application thereof
ActiveCN110441277ARealize highly sensitive detectionAchieve signal amplificationFluorescence/phosphorescenceAmpicillinNanoparticle
The invention relates to the technical field of biosensors, especially relates to a fluorescent biosensor based on hybrid chain reaction amplification, and solves the problems in the prior art that the method for detecting ampicillin is low in specificity and sensitivity, and the cost is high. According to a biosensor for detecting the ampicillin based on an aptamer, a cyclic amplification effectis realized by the cooperation of Nb.BbcCI and a chain hybrid chain reaction, and Thioflavine-T and G-quadruplex are combined to generate fluorescence and a homogeneous reaction mixed solution. The preparation method comprises the following steps of preparing gold nanoparticles; modifying Walker and Track to the surfaces of the gold nanoparticles; mixing a labeled nanogold solution with the homogeneous reaction solution; performing hyperbranched hybrid chain reaction and fluorescent detection; carrying out high specificity detection on the target ampicillin by using the aptamer by utilizing the specific recognition of the aptamer; and using hyperbranched hybrid chain reaction amplification to achieve signal amplification.
Owner:UNIV OF JINAN
Fluorescent probe for targeting detection and cancer cell inhibition and its preparation method and use
InactiveCN104262373AEasy to prepareEasy to operateGroup 3/13 element organic compoundsFluorescence/phosphorescenceCancer cellSuzuki reaction
The invention discloses a fluorescent probe for targeting detection and cancer cell inhibition and its preparation method and use, and belongs to the field of fluorescent biosensors. The fluorescent probe is a 1,4-tetraarylbutadiene derivative containing 4, 4', 4''-di-4-difluoro-4-borata-3a-azonia-4a-aza-s-indacene (BODIPY). The preparation method comprises that 4,4'-((1E, 3E)-1,4-dibromo-1,3-butadienyl-1,4-dily)dibenzaldehyde (or methyl 4,4'-((1E, 3E)-1,4-dibromo-1,3-butadienyl-1,4-diyl)dibenzoate) and BODIPY pinacol ester undergo a Suzuki reaction in the presence of a tetrakis(triphenylphosphine)palladium as a catalyst to produce the fluorescent probe or BODIPY-DICHO and a biomolecule undergo a one-step Schiff base reaction to produce the fluorescent probe. The preparation method has simple processes and is convenient for operation. The fluorescent probe has functions of targeting marking and cancer cell inhibition and can be used as a latent anticancer drug.
Owner:BEIJING INSTITUTE OF TECHNOLOGYGY
Fluorescent biosensor for detecting ochratoxin A as well as preparation method and application thereof
ActiveCN109444102AStrong specificityThe detection process is fastFluorescence/phosphorescenceOchratoxin AFluorescence biosensor
The invention relates to the technical field of biosensors, and particularly relates to a fluorescent biosensor for detecting ochratoxin A based on a rolling ring amplification mediated catalytic hairpin self-assembly and incision enzyme feedback amplification method. According to the fluorescent biosensor, as for a detection mode, a fluorescence method is adopted for detection, and a luminoscopeis utilized; before detection, a padlock probe and a connecting probe form an annular template probe; then a target object is added to a homogeneous solution of a complex probe I, a complex probe II,HP 2 and HP 3, incubating is carried out for 120 minutes at 37 DEG C, and the target object and an aptamer sequence are bound; the multiple feedback amplification process is completed under the actionof a phi29 DNA polymerase and an endonuclease IV, so that signal amplification is realized; and then, the luminoscope is used for setting the excitation wavelength to be 399 nm, the fluorescence intensity at 610 nm is detected, and the detection range is 560-640 nm. Meanwhile, the invention further provides a preparation method for the biosensor. The preparation method has the advantages of beingmild in reaction condition and easy to operate.
Owner:UNIV OF JINAN
Composite fluorescent biosensor, and preparation method and application thereof
ActiveCN107632002AEnables fluorescence detectionAccurate detectionFluorescence/phosphorescenceCelluloseMicrosphere
The invention relates to a composite fluorescent biosensor, and a preparation method and an application thereof. The composite fluorescent biosensor comprises a graphene quantum dot and a carboxymethyl cellulose-chitosan polyelectrolyte microsphere; and the carboxymethyl cellulose-chitosan polyelectrolyte microsphere is a microsphere having a core-shell structure, the core is made of melamine andformaldehyde, and the shell is made of chitosan and carboxymethyl cellulose. The preparation method of the organic-inorganic composite fluorescent biosensor and a fluorescence detection method of target biological polysaccharides are helpful for improving other biological sensors. The composite fluorescent biosensor formed by cellulose ether-based polyelectrolyte microsphere induced graphene quantum dot aggregation has a very good market prospect and very good industrial production values.
Owner:BEIJING INSTITUTE OF TECHNOLOGYGY +2
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