The invention discloses a fluorescent
reagent for detecting trace gamma-
globulin, as well as a preparation method and application thereof, and belongs to the field of fluorescent bio-sensors. The fluorescent
reagent is prepared from TABD-Py-PF6 and a good
solvent. The preparation method comprises the following steps: dissolving (1Z,3Z)-1,4-di(4-methoxycarbonyl) phenyl-1,4-dibromo-1,3-butadiene in the good
solvent, adding 4-
pyridine phenylboronic acid, a catalyst and a basic salt, and reacting to obtain TABD-Py; dissolving the TABD-Py in a
solvent, adding iodomethane to synthesize
iodate, adding
potassium hexafluorophosphate, and reacting to obtain TABD-Py-PF6; dissolving the TABD-Py-PF6 in the good solvent to obtain the fluorescent
reagent. The fluorescent reagent does not need participation of
metal ions, has a specific lighting-type fluorescent response on gamma-
globulin, has high and quick sensitivity on gamma-
globulin detection, and has
high selectivity. The fluorescent reagent has an excellent visible detection
signal, can be completely separated from high-precision instruments according to actual requirement, and can meet current requirement for detecting the gamma-globulin in serum to a great extent.