Fluorescent reagent for trace serum albumin, and preparation and application of fluorescent reagent
The technology of serum albumin and fluorescent reagent is applied in the field of fluorescent biosensors, which can solve the problem of low selectivity of serum albumin detection, and achieve the effects of fast detection, simple preparation method and convenient operation.
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Embodiment 1
[0050] (1) Preparation of DP-TPP
[0051] Dissolve 100 mg of 3,4-dibromo-1,2,5-tris(methoxycarbonylphenyl)pyrrole in 10 mL of ethylene glycol dimethyl ether, add phenylboronic acid (192 mg), tetrakis(triphenylphosphine) Palladium (18mg) and cesium carbonate (225mg) were reacted at 70° C. for 18 hours to obtain a white solid powder; the proton nuclear magnetic spectrum and mass spectrometry data of the white solid powder obtained by nuclear magnetic resonance spectrometer and mass spectrometer were as follows:
[0052] 1 H-NMR (400MHz, CDCl 3 )δ(ppm):7.91-7.71(d,6H),7.22-7.04(d,6H),6.99-6.84(d,10H),3.94-3.84(s,9H).MS(MALDI-TOF):calcd .for C 40 h 31 NO 6 ,621.2;found,621.3;
[0053] It shows that the white solid powder is DP-TPP according to the present invention.
[0054] (2) Preparation of DP-TPPNa
[0055] Dissolve 100 mg of DP-TPP in a mixed solvent of water (5 mL) and tetrahydrofuran (20 mL), add sodium hydroxide (58 mg), heat up to 70 ° C for 18 hours, freeze and v...
Embodiment 2
[0069] (1) Preparation of DP-TPP
[0070] Dissolve 100 mg of 3,4-dibromo-1,2,5-tris(methoxycarbonylphenyl)pyrrole in 30 mL of ethylene glycol dimethyl ether, add phenylboronic acid (222 mg), tetrakis(triphenylphosphine) Palladium (55mg) and cesium carbonate (364mg) were reacted at 70° C. for 30 hours to obtain a white solid powder; the NMR and mass spectrometry data of the white solid powder obtained by NMR spectrometer and mass spectrometer were as follows:
[0071] 1 H-NMR (400MHz, CDCl 3 )δ(ppm):7.91-7.71(d,6H),7.22-7.04(d,6H),6.99-6.84(d,10H),3.94-3.84(s,9H).MS(MALDI-TOF):calcd .for C 40 h 31 NO 6 ,621.2;found,621.3;
[0072] It shows that the white solid powder is DP-TPP according to the present invention.
[0073] (2) Preparation of DP-TPPNa
[0074]Dissolve 100mg of DP-TPP in a mixed solvent of water (40mL) and tetrahydrofuran (20mL), add sodium hydroxide (96mg), heat up to 70°C for 30 hours, freeze and vacuum dry after dialysis to obtain a white solid powder; ...
Embodiment 3
[0088] (1) Preparation of DP-TPP
[0089] Dissolve 100 mg of 3,4-dibromo-1,2,5-tris(methoxycarbonylphenyl)pyrrole in 20 mL of ethylene glycol dimethyl ether, add phenylboronic acid (200 mg), tetrakis(triphenylphosphine) Palladium (40mg) and cesium carbonate (280mg) were reacted at 70° C. for 24 hours to obtain a white solid powder; the NMR and mass spectrometry data of the white solid powder obtained by NMR spectrometer and mass spectrometer were as follows:
[0090] 1 H-NMR (400MHz, CDCl 3 )δ(ppm):7.91-7.71(d,6H),7.22-7.04(d,6H),6.99-6.84(d,10H),3.94-3.84(s,9H).MS(MALDI-TOF):calcd .for C 40 h 31 NO 6 ,621.2;found,621.3;
[0091] It shows that the white solid powder is DP-TPP according to the present invention.
[0092] (2) Preparation of DP-TPPNa
[0093] Dissolve 100mg of DP-TPP in a mixed solvent of water (15mL) and tetrahydrofuran (15mL), add sodium hydroxide (70mg), heat up to 70°C for 24 hours, freeze and vacuum dry after dialysis to obtain a white solid powder; ...
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