116results about How to "Highly selective" patented technology

The present invention is a process for separating a target protein (such as a recombinant protein produced in a cell culture) from a mixture containing the target protein and contaminants (such as cell culture contaminants), by contacting the mixture with a hydrophobic adsorbent comprising branched hydrocarbon functional groups in an aqueous salt solution and collecting the unbound flow-through fraction containing the target protein. In one embodiment, the hydrophobic adsorbent may be a branched alkyl functional group. In another embodiment, the branched alkyl functional group has from 3 to 8 carbon atoms. In another embodiment, the branched alkyl functional group is a tertiary carbon atom, such as tert-butyl.

Strategies, systems, methods, reagents, and kits for phage-assisted continuous evolution are provided herein. These include strategies, systems, methods, reagents, and kits allowing for stringency modulation to evolve weakly active or inactive biomolecule variants, negative selection of undesired properties, and / or positive selection of desired properties.

Methods for cell lysis and purification of biological materials, involving subjecting a sample to high pressure. Also featured is an apparatus for practicing the methods.

The invention discloses a polypeptide with improved alkaline stability, which polypeptide comprises a mutant of a B or C domain of Staphylococcus Protein A (SpA), as specified by SEQ ID NO 1 or SEQ ID NO 2, or of Protein Z, as specified by SEQ ID NO 3, comprising at least the mutation wherein the glutamine residue at position 9 has been mutated to a tryptophan, leucine, glutamic acid, valine or lysine. The invention also discloses multimers of the polypeptide, as well as separation matrices comprising the multimers or polypeptides.

An Fc-binding polypeptide of improved alkali stability, comprising a mutant of an Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO:3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:22, SEQ ID NO 51 or SEQ ID NO 52 wherein at least the asparagine or serine residue at the position corresponding to position 11 in SEQ ID NO:4-7 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucine, isoleucine, tryptophan, methionine, valine, alanine, histidine and arginine.

The invention discloses a method of utilizing replacement control and herbicide application to control mikania micrantha, and belongs to the technical field of comprehensive prevention and control to the exotic invasion pest. The method comprises the following steps: when mikania micrantha is emerged and grows for 30 days, planting and sticking simple-link sweet potato seedlings with length of 5-7 cm for 10 days between the mikania micrantha seedlings, and at the same time, spraying bentazone at the dosage of 48-72 g / mu. Compared with the single application of bentazone, 60-90 days after administration, the control effect is improved by 2.52-20.44%; compared with the single sweet potato replacement control, 60-90 days after administration, the control effect is improved by 14.12-38.39%; compared with the single application of bentazone at the dosage of 72 g / mu, the dosage is reduced by 33% when the planting density ratio of the sweet potato to mikania micrantha is 2.6:1, and the control effect is improved by 0.2-1.51%, 60-90 days after administration. The method has the characteristics of high efficiency, safety and long-lasting control.

The present invention is a process for separating a target protein (such as a recombinant protein produced in a cell culture) from a mixture containing the target protein and contaminants (such as cell culture contaminants), by contacting the mixture with a hydrophobic adsorbent comprising branched hydrocarbon functional groups in an aqueous salt solution and collecting the unbound flow-through fraction containing the target protein. In one embodiment, the hydrophobic adsorbent may be a branched alkyl functional group. In another embodiment, the branched alkyl functional group has from 3 to 8 carbon atoms. In another embodiment, the branched alkyl functional group is a tertiary carbon atom, such as tert-butyl.

A polypeptide with improved alkaline stability, which polypeptide comprises a mutant of a B or C domain of Staphylococcus Protein A, as specified by SEQ ID NO 1 or SEQ ID NO 2, or of Protein Z, as specified by SEQ ID NO 3, wherein at least the glutamine residue at position 9 has been mutated to an amino acid other than asparagine. The invention also discloses multimers of said polypeptide, as well as separation matrices comprising the multimers or polypeptides.

A separation membrane comprising a polyether polymer having a weight-average molecular weight of 104 to 107 and obtained by polymerizing an oxirane compound of the formula: wherein R is a hydrogen atom; an alkyl group, an alkyl group having an halogen atom, or -CH2O(CH2CH2O)k-R' (R' is a group selected from an alkyl group, an alkenyl group, a cycloalkyl group, an aryl group and an aralkyl group; k is from 0 to 12.); or an ethylenically unsaturated group, a reactive silicon-containing group or a methylepoxy-containing group, is excellent in mechanical strength and flexibility.

The invention relates to an ultraviolet detector, in particular to an ultraviolet detector with high spectral selectivity and high sensitivity and a preparation method of the ultraviolet detector. A beta-Ga<2>O<3> film is deposited on an n-type 4H-SiC substrate through a laser molecular beam epitaxy technique; and a Ti / Au film is deposited on the n-type 4H-SiC substrate and the beta-Ga<2>O<3> film for use as an electrode by a mask through a radio-frequency magnetron sputtering technique. The ultraviolet detector has the advantages that the prepared ultraviolet detector has stable performance, has high selectivity and high sensitivity on an ultraviolet spectrum with a specific wavelength, is low in dark current, and can be applied to fire alarm, high-voltage line corona and detection of the spectrum with the specific wavelength; furthermore, the preparation method has the characteristics of being high in process controllability, simple in operation, good in universality and the like and has a great application prospect; and a repeated test has recoverability.

Disclosed are A3 adenosine receptor antagonists and / or partial agonists of formula (I): wherein R1 to R5 are as described herein, as well as pharmaceutical compositions thereof and methods of use thereof. The antagonists or partial agonists find use in treating a number of diseases including cancer, glaucoma, inflammatory diseases, asthma, stroke, myocardial infarction, allergic reactions, rhinitis, poison ivy induced responses, urticaria, scleroderma, arthritis, brain arteriole diameter constriction, bronchoconstriction, and myocardial ischemia, as well as in preventing cardiac ischemia. Also disclosed are radiolabeled compounds of formula (I) and the use thereof in diagnostic imaging of tissues and organs.

The present invention relates to a catalyst for reducing mercury, which comprises a reagent comprising any of the sulfites of potassium, sodium, calcium and magnesium, or any of the phosphates thereof, or a combination of them, as a main reagent of a catalyst component. And the present invention relates to the catalyst for reducing mercury, wherein the catalyst component is mixed with a different salt as an agent for inhibiting crystallization of the catalyst component.

A method of isolating semiconductor devices by wet etching of a semiconductor laminate structure formed on a substrate includes providing an etching stop layer having at least two layers between the substrate and the semiconductor laminate structure. The semiconductor laminate structure is etched to isolate the semiconductor devices, the substrate is then etched away, followed by sequentially etching away of the etching stop layer.

The present invention provides a class of 2,4-substituted phenylene-1,5-diamine derivatives, having an inhibiting effect on EGFR tyrosine kinases, and pharmaceutically acceptable salt, stereoisomer, solvate or prodrug of said derivatives. See the description for the definition of each group in the formula. In addition, the present invention also discloses pharmaceutical compositions, pharmaceutically acceptable compositions and applications thereof.

The present invention is directed to methods for preparing hydrous iron oxide spherules, hydrous iron oxide gels such as gel slabs, films, capillary and electrophoresis gels, iron monohydrogen phosphate spherules, hydrous iron oxide spherules having suspendable particles homogeneously embedded within to form composite sorbents and catalysts, iron monohydrogen phosphate spherules having suspendable particles of at least one different sorbent homogeneously embedded within to form a composite sorbent, iron oxide spherules having suspendable particles homogeneously embedded within to form a composite of hydrous iron oxide fiber materials, iron oxide fiber materials, hydrous iron oxide fiber materials having suspendable particles homogeneously embedded within to form a composite, iron oxide fiber materials having suspendable particles homogeneously embedded within to form a composite, dielectric spherules of barium, strontium, and lead ferrites and mixtures thereof, and composite catalytic spherules of barium or strontium ferrite embedded with oxides of Mg, Zn, Pb, Ce and mixtures thereof. These variations of hydrous iron oxide spherules and gel forms prepared by the gel-sphere, internal gelation process offer more useful forms of inorganic ion exchangers, catalysts, getters, dielectrics, and ceramics.

The invention relates to a separation matrix comprising at least 11 mg / ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg / ml.

The invention discloses kappa light chain-binding polypeptide comprising a mutated binding domain of Peptostreptococcus protein L, wherein at least one asparagine residue of a parental domain defined by, or having at least 95% or 98% sequence homology with, SEQ ID NO: 2-6 or 2 has been mutated to another amino acid residue which is not asparagine, proline or cysteine.

The invention relates to a method of isolating an immunoglobulin, comprising the steps of:a) providing a separation matrix comprising at least 15 mg / ml multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, wherein the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg / ml;b) contacting a liquid sample comprising an immunoglobulin with the separation matrix;c) washing the separation matrix with a washing liquid;d) eluting the immunoglobulin from the separation matrix with an elution liquid; ande) cleaning the separation matrix with a cleaning liquid comprising at least 0.5 M NaOH.

Provided is a method for producing a rice F1 seed including crossing a rice male sterile line of Koshihikari containing one or more genes selected from the group consisting of the Pb1 gene derived from a rice (Oryza sativa L) cultivar Modan and the Cr1 gene derived from Oryza nivara, as a seed parent, with a rice fertility restorer line as a pollen parent, and collecting the first filial generation seed (F1 seed) from the post-crossing seed parent; a rice F1 seed which is obtained by the above-described method for producing a rice F1 seed; and a rice cytoplasmic male sterile line including one or more genes selected from the group consisting of the Pb1 gene derived from rice (Oryza sativa L) cultibar Modan, and the Cr1 gene derived from Oryza nivara.

The present invention relates to the calibration of devices using a secondary binding agent or reference material. In one embodiment, the present invention provides a method of calibrating a nanosensor by providing a nanosensor comprising an analyte binder attached to a reference binder, extracting a calibration curve from binding a reference material to the reference binder, and calibrating the nanosensor by using the calibration curve to correct for device variation.

The invention discloses a preparation method of R-3-aminobutyric acid. The method comprises the following steps: taking crotonic acid and ammonium salt as substrates; adding salt containing magnesiumions and regulating the pH (Potential of Hydrogen) by utilizing ammonia water; then adding recombinant aspartase as a biological enzyme catalyst; reacting at proper temperature and under an alkaline condition; after reacting, separating, purifying and crystallizing to obtain the R-3-aminobutyric acid. According to the method disclosed by the invention, ammonium ions of a reaction system are controlled, and reverse reaction is controlled and generation of byproducts is reduced; impurities including bacterial sludge, zymoprotein, sulfate ions, pigments and the like are intercepted by utilizing microfiltration and nanofiltration; high-quality products with high chiral purity and high impurity purity are obtained through continuous concentration and crystallization.

Provided is a facilitated CO2 transport membrane having an improved CO2 permeance and an improved CO2 / H2 selectivity. The facilitated CO2 transport membrane includes a separation-functional membrane that includes a hydrophilic polymer gel membrane containing a CO2 carrier and a CO2 hydration catalyst. Further preferably, the CO2 hydration catalyst at least has catalytic activity at a temperature of 100° C. or higher, has a melting point of 200° C. or higher, or is soluble in water.

The present invention is a method and apparatus for treating a substrate using an impingement cryogenic or steam spray jet whereas a substrate is first encased in a prophylactic device whereupon the substrate is continuously bathed in a counterflowing stream of ULPA-filtered, inert, dry, heated and ionized gas which bathes both the posterior and anterior regions of the critical substrate surface to be treated-precluding the intrusion of contaminating atmospheres onto critical surfaces. This creates an in-situ microenvironment within which the substrate surface is treated with said impinging treatment spray. The treated surface is thus isolated and protected from the ambient atmosphere and contaminants contained therein prior to, during and following application of a treatment spray without causing a direct impingement of a protective atmosphere upon a critical surface. The critical substrate surfaces being treated are continuously supplied heat, ions and a diluting atmosphere through molecular diffusion phenomenon rather than through direct impingement purging, drying or ion bombardment, thereby minimizing or eliminating the creation or introduction of contaminants into or within the cleaning zone. The present invention allows for the simultaneous application of a variety of conventional surface treatment agents such as dry steam or snow to a critical surface while controlling and maintaining the quality of the environment immediately within the vicinity of the critical surface being treated.

The invention discloses a preparation method of an absorbent for effectively reducing phenol content in cigarette smoke. The preparation method comprises the following steps: preparing a phenol imprinting polymer by molecular imprinting technology and a bulk polymerization method, and obtaining the absorbent of which surface is porous and contains a plurality of holes matched with phenol molecules in structure and shape; and the absorbent has high selectivity to phenol. The absorbent is added into a ternary composite rod, the addition amount can be regulated as required, when the addition amount is 10 mg / cigarette, compared with a comparing sample, the reduction rate of phenol in cigarette smoke is 17.8%, and the relative reduction rate relative to tar is 11.8%; and when the addition amount is 20 mg / cigarette, the reduction rate of phenol in cigarette smoke is 28.7%, and the relative reduction rate relative to tar is 12.3%. The method is simple to operate, low in cost and convenient for a large-scale industrial production.

The invention relates to a separation matrix comprising at least 11 mg / ml Fc-binding ligands covalently coupled to a porous support, wherein:a) the ligands comprise multimers of alkali-stabilized Protein A domains, andb) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg / ml.

A crystalline titanosilicate catalyst which is usable as a catalyst in the oxidation reaction of a compound having a carbon-carbon double bond and at least one other functional group, a process for producing the catalyst, and a process for producing an oxidized compound by an oxidation reaction using the catalyst. It has been found that a crystalline titanosilicate having a structural code of MWW effectively functions as a catalyst in an oxidation reaction of a compound having a carbon-carbon double bond and at least one other functional group, or a compound having a carbon-carbon double bond a functional group and having a total carbon number of not smaller than 2 and not larger than 5, wherein the carbon-carbon double bond of the compound is oxidized by using a peroxide as an oxidizing agent, thereby to highly selectively provide an intended oxidized compound.

The invention discloses a preparation method of an adsorbent capable of reducing nicotine content in cigarette smoke. The preparation method comprises the following steps: performing molecular imprinting by a sacrifice silica gel skeleton method, after imprinting, removing silica gel by using hydrofluoric acid to solute, and obtaining microspheres with a molecular recognition function. The microspheres basically keep the shapes of primary silica gel microspheres, and have greater hole volumes and specific surface areas, a plurality of reaction sites and higher selectivity and adsorption capacity. The absorbent is applied to a composite rod, the addition amount can be regulated as required, when the addition amount is 10 mg / cigarette, compared with a comparing sample, the reduction rate of nicotine in cigarette smoke is 23.8%, and the relative reduction rate relative to tar is 10.4%; and when the addition amount is 20 mg / cigarette, compared with a comparing sample, the reduction rate of nicotine in cigarette smoke is 43.7%, and the relative reduction rate relative to tar is 23.4%; thus the effect of selectively reducing nicotine is very obvious.