The invention belongs to the technical field of gene engineering, and particularly relates to a TCR primer group for specifically recognizing an EBV peptide fragment with immune typing of HLAA02 and application of the TCR primer group. The primer group provided by the invention is designed according to HLAA02 typing and TCR of an EBV virus antigen peptide fragment FLYALALLL, and comprises one or more of TCR primer pairs with the loci of L2-1, L2-2, L2-3, L2-5, L2-6, L2-9, L2-10, L2-11, L2-12, L2-13, L2-16, L2-19, L2-23, L2-24 and L2-25. The sequences of the primers disclosed by the invention are as shown in SEQ ID NO. 1-30. The primer group disclosed by the invention can be used for specifically recognizing the TCR of the EBV antigen peptide fragment FLYALALL in the body of an HLAA02 typing patient.