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Fluorescent biosensor, preparation method and application of fluorescent biosensor to detect organic phosphorus pesticide

The invention provides a fluorescent biosensor, a preparation method and an application of the fluorescent biosensor to detect an organic phosphorus pesticide. The luminous effect of copper nanoparticles which take polythymine-carrying single-chain DNA as a template is used, tyrosinase is used to quench the copper nanoparticles, and the organic phosphorus pesticide is added to inhibit the activityof tyrosinase, thereby preparing the fluorescent biosensor which is constructed based on copper nanoparticles which take polythymine-carrying DNA as a template, tyrosinase and the organic phosphoruspesticide. The fluorescent biosensor can detect the organic phosphorus pesticide with sensitivity and specificity. Compared with a fluorescent biosensor in the prior art, the fluorescent biosensor adopts unmarked DNA, is simple to operate and low in cost, and is free of any chemical mark and modification. Through enzymatic activity inhibition, a sensor capable of detecting an organic phosphorus pesticide can be prepared. The result shows that the sensor can detect the organic phosphorus pesticide with the sensitivity being 0.1 ng / L to 1000 ng / L. The fluorescent biosensor is simple to operate,high in sensitivity and low in detection limit.
Owner:ANHUI NORMAL UNIV
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