50results about How to "Enables fluorescence detection" patented technology

The invention provides a Zn<2+> ratiometric fluorescent probe compound and a preparation method and use thereof, and belongs to the field of fluorescent probe compounds and preparation thereof. According to the preparation method, the Zn<2+> ratiometric fluorescent probe compound prepared by using 2-(4'-amino-2'-hydroxyphenyl)benzoxazole as a raw material has high water solubility. The compound can be used to test Zn<2+> concentration in a water phase. A 1*10<-6> to 1*10<-4>mol/L aqueous solution of Zn<2+> ratiometric fluorescent probe compound can test the strength of the fluorescence peaks at 370nm and 454nm in fluorescence spectrum, and accordingly the Zn<2+> concentration of the aqueous solution can be worked out. The compound provided by the invention has high selectivity in Zn<2+> test and high reproducibility, and therefore is suitable for test the Zn<2+> concentration in water phases in biochemistry and environmental chemistry.

The invention provides a capillary array electrophoresis detection method based on spatial correction and spectral correction, and the method comprises the following steps: (1) utilizing a Raman spectrum to position imaging positions of all capillaries in a capillary array on a charge coupled element; (2) performing spectrum unscrambling on multi-color fluorescent dyes in each capillary through the spatial correction; and (3) performing analysis on DNA (deoxyribonucleic acid) fragments of electrophoresis of the capillary array. In the method, the Raman spectrum of laser excitation capillary quartz or solvent water in the capillaries is adopted for realizing spectral imaging, thereby realizing the position positioning and parallelism adjustment of the capillaries on a CCD (charge coupled device); a fluorescent dye combined matrix is adopted for realizing simultaneous detection of the multi-color fluorescent dyes in the single capillary and the pixel combination way is adopted for improving the stability of a distribution matrix, reducing the amount of calculation and improving the detection stability; and a device for implementing the method is simple in structure and easy to operate.

The invention discloses a fluorescent aptamer probe based on Prussian blue nanoparticles as well as a preparation method and application of the fluorescent aptamer probe. The fluorescent aptamer probeis composed of Prussian blue nanoparticle complex modified FAM fluorophore aptamers. The preparation method comprises the following steps: incubating the Prussian blue nanoparticles and the aptamersmodified with the FAM fluorophore in an HEPEs buffer solution system in a dark place, and closing by adopting BSA, thereby obtaining the fluorescent aptamer probe. The fluorescent aptamer probe can beused for detecting various markers such as markers of tumor, breast cancer, blood glucose and Alzheimer's disease, has the advantages of strong signal, high specificity, high sensitivity, wide detection concentration range, good biological safety and the like, and is beneficial to popularization and application.

The invention belongs to the technical field of biology, and particularly relates to a molecular marker detection method, and particularly relates to a method for detecting molecular markers based ona KASP technology. The molecular markers are non-SNP and InDels. The method comprises the steps that KASP primers are designed in marker amplification target DNA sequences; for a co-dominant marker, three primers need to be designed for two target DNA sequences, and the three primers comprise two site specific primers and one common primer; for a dominant marker, two primers need to be designed for only one target DNA sequence, and the two primers comprise one site specific primer and one common primer; and the 3' end of each site specific primer is an SNP site, the Tm value of the primers is1 degree or above higher than that of a KASP joint sequence, and the size of an amplification fragment is 170 +/-130bp. According to the method, the detection range of the KASP technology is widened,and STS or SCAR is quickly detected.

The invention discloses a ratiometric fluorescent probe compound and a method used for detecting zinc ion ratio in aqueous solution, and belongs to the field of fluorescent probe. The chemical molecular formula of the ratiometric fluorescent probe compound used for detection zinc ions is C20H16N3O3Cl, and is prepared through derivatization of 2-(2'-hydroxyphenyl) benzoxazole compound which is taken as a matrix structure. The ratiometric fluorescent probe compound is high in selectivity on zinc ions, wide in detection range, high in sensitivity, excellent in anti-influence capacity on other metal ions, and is suitable for detection of Zn<2+> in aqueous solutions in the fields of biochemistry and environment chemistry.

The invention discloses a detection device adopting lased-induced liquid fluorescence and relates to the technical field of fluorescence spectra. The detection device comprises an automatic injected sample cleaning system, a fluorescence excitation system and a beam splitting system, wherein the automatic injected sample cleaning system is located in the middle of the whole device, and the fluorescence excitation system and the beam splitting system are located on two sides of the automatic injected sample cleaning system respectively. A liquid-core optical fiber is filled with a to-be-measured liquid, fluorescence produced by excitation is transmitted in the liquid-core optical fiber along with incident light and accumulated and intensified continuously, a laser is all limited in the whole liquid-core optical fiber to be transmitted after coupled to enter the liquid-core optical fiber, exciting light interacts with the to-be-detected liquid continuously, the total effective exciting light path is extended, and the intensity of the fluorescence produced by excitation is accumulated continuously. The liquid-core optical fiber can be very long, so that the intensity of the fluorescence can be increased substantially in the optical fiber, and fluorescence detection of low-concentration substances can be realized.

The invention discloses an enhanced fluorescence probe for detecting carboxylesterase 1 (CES1) as well as a preparation method and application thereof, and belongs to the technical field of fluorescence probe materials. The fluorescence probe refers to 3-bromomethyl-2-oxo-2H-chromen-7-acetate, and the preparation method comprises the flowing steps of (1) dissolving 3-methyl-2-oxo-2H-chromen-7-acetate, N-bromosuccinimide and azodiisobutyronitrile in carbon tetrachloride to obtain a mixed solution; (2) heating the mixed solution for refluxing, and cooling to room temperature after the reaction, performing separation and purification on a reaction product, so as to obtain the 3-bromomethyl-2-oxo-2H-chromen-7-acetate. The fluorescence probe can be used for realizing fluorescence enhanced detection on the CES1; has the advantages of effectiveness, perceptual intuition and easiness in observation; is high in stability, and capable of avoiding interference of external conditions; improves the accuracy of the detection; and is capable of being widely applied to the qualitative and quantitative analysis on the CES1 in environment samples, chemical samples and the like.

The invention relates to an active targeting type ultrasonic/fluorescence bimodal contrast medium as well as a preparation method and application thereof. Specifically, the contrast medium comprises nano microbubbles and targeting molecules, wherein the nano microbubbles are filled with an inner water phase; and the targeting molecules are coupled with the surfaces of the nano microbubbles. The invention further discloses a preparation method and application of the contrast medium. The contrast medium is excellent in biocompatibility and relatively small in particle size, and can not only exist and be used stably, but also effectively permeate into tumor cells, and thus targeting radiography upon tumor cells is achieved. The preparation method is simple in process and economic and safe.

The invention relates to a fluorescent probe molecule for detecting diaphorase based on a rhodamine derivative and a preparation method and application thereof. The molecular structure of the fluorescent probe is shown in figure 1. The invention also discloses a preparation method of the fluorescent probe molecule for detecting the diaphorase based on the rhodamine derivative, and application of the fluorescent probe molecule in detecting the diaphorase in a water body sample or biological cells. The fluorescent probe molecule synthesis method is easy to synthesize and high in yield.

The invention discloses a triarylboron conjugated polymer porous material as well as a preparation method and application thereof. The invention provides a novel preparation method of a triaryl conjugated polymer porous material, i.e., the novel triaryl conjugated polymer porous material is prepared by using a boron-tin exchange reaction as a polymerization means. The defects of high cost, difficulty in purification and the like in a preparation method of carbon-carbon coupling reaction catalyzed by noble metal are overcome, and the method is more economical and efficient. The triaryl conjugated polymer porous material disclosed by the invention can realize fluorescence detection on organic ammonia, the fluorescence quenching ratios of pyridine to P2-Th, P2-Th2 and P2-Th3 of the triaryl conjugated polymer porous material disclosed by the invention are 83.8%, 73.5% and 41.3% respectively, and the triaryl conjugated polymer porous material disclosed by the invention has a good application prospect in the field of fluorescence detection.

The invention discloses a cysteine detection reagent and a preparation method thereof. The cysteine detection reagent has the general structure as shown in the formula (please see the formula in the description), wherein R1 is H, Cl or OCH3, R2 is H, Cl or OCH3. The detection reagent does not have biological toxicity and is high in sensitivity to cysteine, cysteine can be selectively detected, fluorescent detection can be performed on cysteine in cells, and the cysteine detection reagent meets the commercialized application requirement.

The invention provides a multichannel microfluidic optical detection system in the technical field of fluorescence detection. The multichannel microfluidic optical detection system comprises a light source, a multichannel microfluidic module, a light source coupling control module, a liquid path control module, a light-electricity conversion module and a detection analysis module, wherein the light source is used for outputting optical signals, and one light source is available; the multichannel microfluidic module is provided with at least two microchannels; the light source coupling controlmodule is used for inputting the optical signals to each microchannel in a distribution manner; the liquid path control module is used for controlling the generation frequency and flow velocity of microdrops in each microchannel; the light-electricity conversion module is used for receiving the optical signals passing through the microchannels, generating electric signals, and amplifying the electric signals; and the detection analysis module is electrically connected with the light-electricity conversion module for analyzing the amplified electric signals, and thus a detection result is obtained. For the multichannel microfluidic optical detection system provided by the invention, the single light source is adopted for realizing the detection for multiple channels, and meanwhile, the increase of power of the light source due to the increase of the number of the channels is also avoided.

The invention discloses a compound for saxitoxin fluorescence detection. The compound is selected from at least one of compounds shown as a structural formula 1, wherein R1 is an organic chromophore with a highly conjugated structure, and R2 is a metal chelate of a transition metal or rare earth. By adopting the compound provided by the invention as a probe, fluorescence detection of saxitoxin asparalytic shellfish poisoning can be realized; the probe compound can be synthesized and prepared through a simple chemical reaction and is low in cost, high in detection sensitivity, good in specificity, strong in anti-interference ability, rapid in detection time, high in efficiency and simple to operate without help of large instrument equipment. The compound provided by the invention has a large application prospect on the aspects of ocean detection, aquaculture, life health and the like. (The structural formula 1 is described in the following description).

The invention discloses a preparation method of high-stability perovskite quantum dots, which comprises the following step: preparing a perovskite quantum dot fluorescence sensor by taking lead bromide and cesium bromide as precursors and taking 3-aminopropyltriethoxysilane and oleic acid as ligands at room temperature. The invention also discloses an application of the perovskite quantum dot fluorescence sensor in alcohol-phase tetracycline detection. According to the invention, a supersaturated recrystallization method at room temperature is adopted, the perovskite quantum dot fluorescence sensor is obtained by coating the surface of perovskite quantum dots with a silicon layer with 3-aminopropyltriethoxysilane for modification, oxidation inactivation of the perovskite quantum dots is avoided, and the stability of the perovskite quantum dots is improved, so that the perovskite quantum dot fluorescence sensor is stably dispersed in a high-polarity solvent. Amino groups on the surfaceof the perovskite quantum dot fluorescence sensor and tetracycline molecules are subjected to specific recognition and combination, fluorescence detection of tetracycline in an alcohol phase is achieved, and the perovskite quantum dot fluorescence sensor has the advantages of being high in selectivity and sensitivity.

The invention discloses a water pollution source analysis method, device and equipment based on spectroelectrochemistry, and belongs to the technical field of water treatment.The method comprises the steps that a water sample is collected; acquiring background data of the water sample in a conventional state, wherein the background data comprises first three-dimensional fluorescence spectrum data and first ultraviolet absorption spectrum information; the water sample is subjected to electrochemical disturbance, disturbance data of the water sample in the disturbance state are obtained, and the disturbance data comprise second three-dimensional fluorescence spectrum data and second ultraviolet absorption spectrum data; and analyzing the background data and the disturbance data, and determining a pollution source. According to the method, the active components in the water quality are enhanced by utilizing electrochemical disturbance, so that the excitation spectrum characteristic peak and the intensity of the excitation spectrum characteristic peak shown by the water sample are enhanced, more information is provided for water pollution source analysis based on spectrum electrochemistry to excavate hidden water quality spectrum information, and the identification degree of water quality detection traceability is increased.

The embodiment of the invention discloses a fluorescence detection device, which comprises a light source module, a reaction module and a detection module, wherein the light source module comprises a plurality of monochromatic source components, a plurality of monochromatic source moving tracks, a first driving component and a first control component; the first control component is used for determining a target monochromatic source component from the monochromatic source components based on a selection instruction so as to output a moving instruction to the first driving component, and the first driving component responds to the moving instruction to drive the target monochromatic source component to move to the final position in the corresponding monochromatic source moving track; and the first control component controls the target monochromatic source component to start after the target monochromatic source component moves to the final position so as to output the target exciting light. According to the embodiment of the invention, the plurality of monochromatic light source components are connected with the plurality of sliding rails, the first driving component and the first control component, so that different monochromatic light sources can be selected to provide exciting light with different wavelengths, and fluorescence detection is carried out.

The invention discloses a method for the fluorescence detection of Alzheimer's disease markers based on a silver nano-cluster probe containing repeated AGGGTT sequences. The method comprises the following steps: synthesizing a silver nanocluster by using DNA1 as a template, specifically binding the DNA1 with DNA2, binding the bound DNA1 and DNA2 with copper ions to obtain a DNA1/DNA2-AgNCs-Cu<2+> probe, and using the DNA1/DNA2-AgNCs-<2+> fluorescence probe as a probe for detecting the Alzheimer's disease markers to detect the content of the Alzheimer's disease markers Abeta 1-40 monomers and Abeta1-40 oligomers through the change of fluorescence signals. The method realizes the fluorescence detection of the Alzheimer's disease markers, and has the advantages of stable signal, strong resistance to environmental impact, high sensitivity, low detection limit, good linear relationship, and easiness in operation.

The invention discloses a fluorescent sensor for detecting the gelation degree of Pickering emulsion as well as preparation and application of the fluorescent sensor. The fluorescent sensor has the following structure. The fluorescence sensor prepared by the invention contains an aromatic ring structure capable of freely rotating, can judge the viscosity of the Pickering emulsion according to the intensity of fluorescence signal release, has aggregation-induced emission (AIE) characteristic, can release a strong fluorescence signal in an aggregation state, is suitable for indirect judgment of the gelatinization degree of the Pickering emulsion, can provide an effective reference for the control of the gelation degree of the Pickering emulsion dressing, and has a certain guiding significance for the preparation process of the Pickering emulsion dressing.

The invention discloses a pollution source analysis method and device and a storage medium, and belongs to the technical field of water environment protection and monitoring. The method comprises the following steps: detecting a water sample by adopting a three-dimensional fluorescence spectrum detection method and an electrochemical spectrum detection method to obtain multi-dimensional spectrum data; carrying out dimension reduction processing on the multidimensional spectral data by utilizing an entropy weight method, and respectively calculating distribution weights of atlas matrixes; distributing weights based on the atlas matrix, and constructing a two-dimensional weight matrix; and calculating the similarity between the two-dimensional weight matrix and a weight matrix in a pre-constructed database by adopting an Euclidean distance judgment analysis method, and determining the pollution source. According to the method, the active components in the water quality are enhanced by electrochemical disturbance, more information is provided for water pollution source analysis to excavate hidden water quality spectral information, and the identification degree of water quality detection traceability is increased; and meanwhile, pollution source tracing analysis is performed by using an entropy weight method-Euclidean distance, so that the accuracy of pollution source identification and analysis is improved.