Multi-channel microfluidic optical detection system

Abstract

A multi-channel microfluidic optical detection system in the field of fluorescence detection technology, comprising: a light source for outputting optical signals; the number is one; a multi-channel microfluidic module with no less than two microchannels; light source coupling control The module is used to distribute the input optical signal to each microchannel; the liquid circuit control module is used to control the generation frequency and flow rate of droplets in each microchannel; the photoelectric conversion module is used to receive the optical signal through the microchannel and regenerate an electrical signal and amplify; the detection and analysis module is electrically connected with the photoelectric conversion module, and analyzes the amplified electrical signal to obtain a detection result. The invention adopts a single light source to realize multi-channel detection, and simultaneously avoids increasing the power of the light source due to the increase in the number of channels.

Description

technical field [0001] The invention relates to a technology in the field of fluorescence detection, in particular to a multi-channel microfluidic optical detection system. Background technique [0002] Optical fluorescence detection technology is the most commonly used detection method in DNA detection. Fluorescence detection uses a laser light source. After the photoelectric conversion module receives and detects the change of the optical signal of the micro-droplet after being irradiated by fluorescence, the optical signal is converted into an electrical signal. The detection result is obtained through data collection and algorithm analysis of the electrical signal. [0003] In the prior art, fluorescence detection requires an excitation light source to emit a certain power of irradiation light. After the microdroplets added with fluorescent dyes are irradiated, the DNA in the microdroplets absorbs enough energy to emit detectable fluorescence. However, the change of lig...

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Problems solved by technology

However, the change of light intensity is easily affected by the environment. Once there is interference in the optical path, it will seriously affect the accuracy of the detection results.

In addition, fluorescence detection mostly directly irradiates the laser on the micro-droplets, and the laser light source is slightly shifted due to the influence of the environment, and the detection cannot be completed smoothly, so the requirements for the structural accuracy of the system are very high.

In addition, the light source power required for fluorescence detection is relatively high, so the detection cost is high

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