A compound and detection method for fluorescence detection of saxitoxin
A saxitoxin and fluorescence detection technology, which is applied in chemical instruments and methods, fluorescence/phosphorescence, chemical analysis by titration, etc., can solve the problems of expensive and difficult to obtain toxin antibodies, and achieve fast time and low cost , the effect of huge application prospects
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Embodiment 1
[0056] Example 1: Preparation and Identification of Compound EuL1
[0057] Compound EuL1 was prepared as follows:
[0058]
[0059] Preparation and identification data of compound EuL1:
[0060] Add 1 equivalent of compound 1, 1.5 equivalents of compound 2, 0.1 equivalent of Pd(PPh 3 ) 2 Cl 2 and 0.03 equivalent of CuI, add THF to dissolve it, and react at 45°C. After the reaction was completed, water was added to quench and extracted with ethyl acetate. The extracted organic phase was dried with anhydrous sodium sulfate, and the solvent was removed under reduced pressure. The residue was separated and purified by silica gel column chromatography to obtain compound 3. The identification results of proton nuclear magnetic resonance spectrum and carbon nuclear magnetic resonance spectrum are as follows: 1 H NMR (CDCl 3 ,500MHz):δ8.55(br,1H),7.50(d,J=8.7Hz,2H),7.44(br,1H),7.39(br,1H),6.91(d,J=8.8Hz,2H) ,4.83(br,1H),3.97(t,J=6.6Hz,2H),1.06(t,J=7.4Hz,3H); 13 C NMR (CDCl...
Embodiment 2
[0067] Fluorescent titration of probe EuL1:
[0068] Prepare the detection solution: dissolve the probe compound EuL1 in double distilled water, and the concentration of the probe EuL1 aqueous solution is 50 μM;
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