A compound and detection method for fluorescence detection of saxitoxin

A saxitoxin and fluorescence detection technology, which is applied in chemical instruments and methods, fluorescence/phosphorescence, chemical analysis by titration, etc., can solve the problems of expensive and difficult to obtain toxin antibodies, and achieve fast time and low cost , the effect of huge application prospects

Active Publication Date: 2021-03-30
PEKING UNIV SHENZHEN GRADUATE SCHOOL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Likewise, antibodies to toxins are expensive and difficult to obtain

Method used

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  • A compound and detection method for fluorescence detection of saxitoxin
  • A compound and detection method for fluorescence detection of saxitoxin
  • A compound and detection method for fluorescence detection of saxitoxin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1: Preparation and Identification of Compound EuL1

[0057] Compound EuL1 was prepared as follows:

[0058]

[0059] Preparation and identification data of compound EuL1:

[0060] Add 1 equivalent of compound 1, 1.5 equivalents of compound 2, 0.1 equivalent of Pd(PPh 3 ) 2 Cl 2 and 0.03 equivalent of CuI, add THF to dissolve it, and react at 45°C. After the reaction was completed, water was added to quench and extracted with ethyl acetate. The extracted organic phase was dried with anhydrous sodium sulfate, and the solvent was removed under reduced pressure. The residue was separated and purified by silica gel column chromatography to obtain compound 3. The identification results of proton nuclear magnetic resonance spectrum and carbon nuclear magnetic resonance spectrum are as follows: 1 H NMR (CDCl 3 ,500MHz):δ8.55(br,1H),7.50(d,J=8.7Hz,2H),7.44(br,1H),7.39(br,1H),6.91(d,J=8.8Hz,2H) ,4.83(br,1H),3.97(t,J=6.6Hz,2H),1.06(t,J=7.4Hz,3H); 13 C NMR (CDCl...

Embodiment 2

[0067] Fluorescent titration of probe EuL1:

[0068] Prepare the detection solution: dissolve the probe compound EuL1 in double distilled water, and the concentration of the probe EuL1 aqueous solution is 50 μM;

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Abstract

The invention discloses a compound for saxitoxin fluorescence detection. The compound is selected from at least one of compounds shown as a structural formula 1, wherein R1 is an organic chromophore with a highly conjugated structure, and R2 is a metal chelate of a transition metal or rare earth. By adopting the compound provided by the invention as a probe, fluorescence detection of saxitoxin asparalytic shellfish poisoning can be realized; the probe compound can be synthesized and prepared through a simple chemical reaction and is low in cost, high in detection sensitivity, good in specificity, strong in anti-interference ability, rapid in detection time, high in efficiency and simple to operate without help of large instrument equipment. The compound provided by the invention has a large application prospect on the aspects of ocean detection, aquaculture, life health and the like. (The structural formula 1 is described in the following description).

Description

technical field [0001] The invention relates to the detection field of paralytic shellfish toxin, in particular to a saxitoxin fluorescence detection compound and detection method. Background technique [0002] Paralytic shellfish toxins: a series of biological toxins secreted by some algae that cause red tides can block sodium ion channels in human cells, causing nervous system transmission disorders and resulting in paralytic poisoning. Because of its strong toxicity, there is currently no specific antidote. Humans have a 100% fatality rate when ingesting PSP toxins above a certain limit. Saxitoxin is one of the main toxins of shellfish nerve paralysis poisoning and one of the most toxic marine biotoxins known. The mild poisoning dose to adults is 110 μg, and the lethal dose is 540-1000 μg. [0003] At present, there are many analytical methods for paralytic shellfish toxins, which can be classified into three types: biological, physical and chemical, and immunochemical....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D519/00C09K11/06G01N21/64G01N31/16
CPCC07D519/00C09K11/06C09K2211/182G01N21/6486G01N31/16G01N2021/6432G01N2021/6443
Inventor 李志成叶伟健黄均荣梁振浩
Owner PEKING UNIV SHENZHEN GRADUATE SCHOOL
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