The invention provides a liquid chromatography-tandem mass spectrometry detection method for paralytic shellfish toxins in shellfish. The method comprises the following steps: 1.1, performing sample extraction: adding a formic acid solution into a shellfish sample, carrying out ultrasonic extraction and centrifuging, taking the supernatant, and fixing the volume to obtain a sample extracting solution; 1.2, performing sample purification: adding dichloromethane into the sample extracting solution, uniformly performing mixing, performing centrifuging, taking supernatant, adding the supernatant into an activated C18 solid-phase extraction column, performing eluting, collecting effluent and eluent, performing combining, fixing the volume, performing standing, performing centrifuging, and taking a supernatant filter membrane to obtain a sample detection solution; and 1.3, performing sample detection: taking the sample detection solution, carrying out liquid chromatography-tandem mass spectrometry detection, and performing quantifying by an external standard method. The method belongs to the technical field of food safety detection, simplifies the operation, reduces the consumption of anorganic solvent, has a good sample purification effect, does not need an ultrafiltration step, realizes effective separation and detection of partial structural analogues in PSTs, and widens the detection variety of the PSTs.