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Method for measuring diarrhetic shellfish poisons in shellfishes by use of immunoaffinity purification-liquid chromatography-tandem mass spectrometry

A liquid chromatography and shellfish toxin technology, which is applied in the field of immunoaffinity purification-liquid chromatography tandem mass spectrometry for the determination of diarrheal shellfish toxins in shellfish, and achieves the effects of less matrix interference, high sensitivity and strong purification effect.

Active Publication Date: 2017-05-24
MARINE FISHERIES RES INST OF ZHEJIANG
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Problems solved by technology

As a new and efficient pre-treatment purification technology, immunoaffinity purification technology is being continuously improved and developed, but there is no relevant report on the application of immunoaffinity-LC-MS detection technology to the simultaneous detection of diarrheal shellfish toxins in shellfish

Method used

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  • Method for measuring diarrhetic shellfish poisons in shellfishes by use of immunoaffinity purification-liquid chromatography-tandem mass spectrometry
  • Method for measuring diarrhetic shellfish poisons in shellfishes by use of immunoaffinity purification-liquid chromatography-tandem mass spectrometry
  • Method for measuring diarrhetic shellfish poisons in shellfishes by use of immunoaffinity purification-liquid chromatography-tandem mass spectrometry

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[0025] 1 Materials and methods

[0026] 1.1 Materials and reagents

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Abstract

The invention discloses a method for measuring diarrhetic shellfish poisons in shellfishes by use of immunoaffinity purification-liquid chromatography-tandem mass spectrometry. The method comprises the following steps: extracting a sample by use of an 80% methanol aqueous solution, mixing and diluting with a phosphate buffer solution, and performing immunoaffinity selective special purification, and performing liquid chromatography-tandem mass spectrometry. According to the use characteristics of a diarrhetic immunoaffinity column, parameters of sample liquid, eluate, eluent and the like are optimized; electrospray anions are ionized, a multi-reaction monitoring mode is adopted, and quantification based on an external standard method is achieved. The method is less in matrix interference, strong in purification effect, high in sensitivity and applicable to analysis measurement on diarrhetic shellfish poisons in shellfishes.

Description

technical field [0001] The invention relates to a method for detecting diarrheal shellfish toxin in shellfish, in particular to an immunoaffinity purification-liquid chromatography tandem mass spectrometry method for detecting diarrheal shellfish toxin in shellfish. Background technique [0002] Okadaic Acid (OA) and its derivatives Dinophysistoxins (DTXs) ​​are a class of lipophilic polyether compounds ( figure 1 ), mainly produced by some algae of the toxic red tide algae Pinophylla and Prorocentrum. When toxic microalgae are filtered by bivalve molluscs, OA and DTXs toxins will accumulate in the shellfish, causing shellfish eaters to be poisoned, with symptoms such as diarrhea, nausea, vomiting, and lower abdominal pain. Diarrhetic shellfishpoisons , DSP) is the most widely distributed and most harmful class of components. In addition to causing diarrhea, OA and DTXs toxins can also damage the intestinal mucosa, are tumor promoting factors, and have genotoxicity. The E...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/08
CPCG01N30/08G01N30/88G01N2030/8813
Inventor 严忠雍陈思张小军李佩佩方益
Owner MARINE FISHERIES RES INST OF ZHEJIANG
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