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A method for rapid screening of tea tree resources containing egcg3"me

A tea tree resource and rapid technology, which can be used in measurement devices, instruments, scientific instruments, etc., can solve the problems of scarcity of tea tree resources and few research reports on methylated EGCG, and achieve the effects of long analysis time and simple pretreatment.

Active Publication Date: 2016-07-06
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the scarcity of tea tree resources rich in methylated EGCG, there are few research reports on methylated EGCG in China

Method used

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  • A method for rapid screening of tea tree resources containing egcg3"me

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Experimental program
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Effect test

Embodiment 1

[0029] Taking the tea tree variety resources of the Gaoqiao Tea Experimental Tea Field of the Hunan Tea Research Institute as the investigation object, sampling was taken on March 30, 2014, and a total of 20 samples were collected. The fresh leaves, one bud, two and three leaves of the tea tree were collected, and the tea samples were freeze-dried for 18-24 hours.

[0030] The method for fast screening rich in EGCG3 "Me tea tree resource, specifically comprises the following steps:

[0031] (1) Accurately weigh 0.20g tea sample, grind it into powder, then add 50ml of methanol, heat and reflux for extraction for 10min, filter to obtain the sample solution;

[0032] (2) Accurately weigh 0.5 mg of EGCG, add 1 ml of methanol to dissolve, obtain the EGCG reference substance solution, the concentration is 0.5 mg / ml, as a qualitative and semi-quantitative standard;

[0033] Accurately weigh 0.5 mg of ECG, add 1 ml of methanol to dissolve, and obtain an ECG reference solution with a c...

Embodiment 2

[0041] Taking the tea tree variety resources in Chang'an Tea Base of Hunan Agricultural University as the investigation object, sampling was taken on April 10, 2014, and a total of 15 samples were collected. Fresh leaves, one bud, two and three leaves were collected, and the tea leaves were freeze-dried for 18-24 hours to obtain tea samples.

[0042] The method for fast screening rich in EGCG3 "Me tea tree resources, specifically comprises the following steps:

[0043](1) Accurately weigh 0.20g tea sample, grind it into powder, then add 50ml of methanol, heat and reflux for extraction for 10min, filter to obtain the sample solution;

[0044] (2) Accurately weigh 0.5 mg of EGCG3 "Me, add 1 ml of methanol to dissolve to obtain EGCG3 "Me reference substance solution, concentration 0.5 mg / ml, as qualitative and semi-quantitative standards;

[0045] (3) Take 6 μl each of the reference solution and the sample solution, and place them on the same polyamide thin-layer plate, on the sa...

Embodiment 3

[0048] Taking the tea tree variety resources of the Gaoqiao Tea Experimental Tea Field of the Hunan Tea Research Institute as the investigation object, sampling was taken on March 25, 2014, and a total of 20 samples were collected. The fresh leaves, one bud, two and three leaves of the tea tree were collected, and the tea leaves were freeze-dried for 18-24 hours to obtain tea leaves sample.

[0049] The method for fast screening rich in EGCG3 "Me tea tree resources, specifically comprises the following steps:

[0050] (1) Accurately weigh 0.20g tea sample, grind it into powder, then add 25ml of methanol, heat and reflux for extraction for 20min, filter to obtain the sample solution;

[0051] (2) Accurately weigh 0.5 mg of EGCG3 "Me, add 1 ml of methanol to dissolve to obtain EGCG3 "Me reference substance solution, concentration 0.5 mg / ml, as qualitative and semi-quantitative standards;

[0052] (3) Take 6 μl and 3 μl of the reference substance solution and the sample solution...

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Abstract

The invention relates to the technical field of economic crop germplasm innovation, in particular to a method for rapidly screening a tea tree resource containing EGCG3''Me (Epigallocatechin Gallate). The method comprises the following steps: (1) preparing sample solution; (2) preparing reference substance solution; (3) dotting the sample solution and the reference substance solution on a same polyamide thin layer board, developing with a developing agent, wherein the volume ratio of methyl alcohol, acetic acid and acetone in the developing agent is (8-10):(4-6):3, taking out, drying, scanning, developing, comparing the sample with the reference substance, considering that the sample contains the EGCG3''Me if the sample has the same Rf as the reference substance, and considering that the sample does not contain the EGCG3''Me if the sample does not have the same Rf as the reference substance. The method is simple and rapid in pretreatment, more than ten tea samples can be simultaneously measured each time, and the method is suitable for analysis and measurement of a large number of samples.

Description

technical field [0001] The invention relates to the technical field of economic crop germplasm innovation, in particular to a method for rapidly screening tea tree resources containing EGCG3"Me. Background technique [0002] (-)-Epigallocatechin 3-O(3-O-methyl)gallate (EGCG3″Me) and (-)-Epigallocatechin 3-O(4-O-methyl)gallate Ester (EGCG4″Me) are two important catechin derivatives in tea tree. Studies have found that EGCG3″Me and EGCG4″Me have extremely significant effects on the treatment of allergic reactions such as pollen allergy, and their biological functions have been confirmed in clinical experiments in vitro and in vivo. EGCG3″Me and EGCG3″Me are twice as effective as EGCG in inhibiting the passive skin allergic response in mice. Methylated EGCG can not only inhibit the release of histamine to inhibit the activity of mast cells, but also inhibit the PTK activity of mast cells, inhibit the nerve stimulation induced by FCεRI and directly inhibit the PS / TK activity. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/90
Inventor 王坤波刘仲华黄建安李勤李银花
Owner HUNAN AGRICULTURAL UNIV
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