4 results about "Shellfish toxins" patented technology

The invention provides a PP2A / PDA-ZnO / GCE electrochemical biosensor as well as a preparation method and application of the PP2A / PDA-ZnO / GCE electrochemical biosensor. The sensor comprises a glassy carbon electrode, an immobilized enzyme layer is arranged on the surface of the glassy carbon electrode, and the immobilized enzyme layer comprises protein phosphatase 2A and nano-zinc oxide coated with polydopamine; the mass ratio of the protein phosphatase 2A in the immobilized enzyme layer to the polydopamine-coated nano-zinc oxide is 5: 8-5: 2, and the molar ratio of dopamine hydrochloride used as raw materials of the polydopamine-coated nano-zinc oxide to the nano-zinc oxide is 1: 3-1: 2. The invention also provides a preparation method of the electrochemical biosensor. The electrochemical biosensor can be used for detecting diarrhetic shellfish poison in seawater and marine organisms, especially okadaic acid and dinosporin; the polydopamine-coated nano zinc oxide and the protein phosphatase 2A have a synergistic effect, so that the binding force is strong, the enzyme activity is good, the performance is stable, the reutilization is realized, and the cost is low.

This invention discloses a method and apparatus for mineralizing paralyzing shellfish poisoning by hydroxyl radicals, relating to the field of marine ecological disaster prevention and control. The method involves: oxygen entering an oxygen plasma generator, being ionized and dissociated to generate oxygen-active particle gas, which is injected into a gas-liquid mixer to efficiently generate a •OH solution, and then injected into a liquid-liquid mixer where it is broken down into a large number of tiny droplets; eight sampling ports are evenly spaced after the liquid-liquid mixer to measure the TRO gradient after mixing and dilution; the large number of •OH droplets rapidly mineralize the paralyzing shellfish poisoning while simultaneously killing the toxin-producing dinoflagellates; detection is performed at the eight sampling ports to establish efficacy-time models for •OH mineralization of shellfish poisoning and •OH mineralization of algae, and to determine the threshold values; the acute and chronic toxicity of the shellfish poisoning control group C2 and the treatment group C3 are evaluated, as are the acute and chronic toxicity of the dinoflagellate control group D2 and the treatment group D3; this invention provides a complete solution from source control to end-of-life assessment.

A method for analyzing lipophilic shellfish toxins based on solid-phase extraction using polystyrene nanofibers is disclosed. This method first extracts lipophilic shellfish toxins from the sample using methanol, then uses polystyrene nanofibers as a solid-phase extraction adsorbent to purify the matrix and enrich the lipophilic shellfish toxins in aquatic products. Finally, high-performance liquid chromatography-triple quadrupole tandem mass spectrometry (HPLC-MS / MS) is employed for detection, enabling the detection of 12 lipophilic shellfish toxins in aquatic products. This invention is the first to introduce nanofiber solid-phase extraction into the detection of lipophilic shellfish toxins. Through hydrophobic interactions and π-π interactions between benzene-ring-containing, hydrophobic polystyrene nanofibers and target analytes containing polyethers or macrolides, efficient enrichment and purification are achieved, removing matrix interference and concentrating the target analytes. The method's sensitivity is nearly 100 times higher than that of the national standard method. Furthermore, this method uses less organic solvent, effectively saving sample pretreatment time and improving detection efficiency.

The present application relates to a kind of anti-saxitoxin recombinant rabbit monoclonal antibody and its application, the amino acid sequence of the anti-saxitoxin recombinant rabbit monoclonal antibody includes heavy chain variable region and light chain variable region, respectively as shown in SEQ ID NO:1 And 10 shown in formula.The high sensitivity of it, the lowest saxitoxin can reach 7.3 pg mL ‑1 IC 50 , linear range is 0.0019 ng L ‑1 0.0282 ng L ‑1 ;Meanwhile, it also has high specificity, and other paralytic shellfish toxins and pufferfish toxin are not cross-reacted.The anti-saxitoxin recombinant rabbit monoclonal antibody prepared in the present application, compared with the anti-saxitoxin mouse monoclonal antibody on sale, the antibody provided in the present application is high in detection sensitivity, high in specificity, good in affinity, high in accuracy;It is suitable for immunological analysis kit, immunological test strip, immunoblotting and other clinical and scientific research applications.