Manufacturing method and application of quantitative paralytic shellfish toxin detection test paper

A technology for detecting shellfish toxins and test strips, which can be used in biological testing, measuring devices, and material inspection products. It can solve the problems of large errors, poor reproducibility, and monitoring limitations of paralytic shellfish poisoning in biological methods, and achieve good specificity. , simple operation technology, high sensitivity effect

Active Publication Date: 2020-10-16
FISHERIES RES INST OF FUJIAN
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the commonly used shellfish poisoning monitoring methods at home and abroad are mainly the biological mouse method and high performance liquid chromatography (HPLC), but the biological method has the disadvantages of large errors, poor reproducibility, and the inability to analyze specific comp

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Manufacturing method and application of quantitative paralytic shellfish toxin detection test paper
  • Manufacturing method and application of quantitative paralytic shellfish toxin detection test paper
  • Manufacturing method and application of quantitative paralytic shellfish toxin detection test paper

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0028] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention clearer and clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0029] Such as figure 1 Shown, a kind of preparation method of quantitative paralytic shellfish toxin detection test paper is as follows:

[0030] The carrier pad 7, the nitrocellulose membrane 8, the sample combination pad 1, the adhesive tape layer 2 and the absorbent pad 6 are spliced ​​into a layered structure: first, the carrier pad 7 is placed on the bottom layer, and the nitrocellulose membrane 8 is laid on the carrier pad 7, Then the sample combination pad 1 and the water-absorbing pad 6 are respectively arranged on the two ends of the nitrocellulose membrane 8, and one end of the sample combination pad 1 and the water-absorbing pad 6 partially overlaps with the nitrocellulose membrane 8 respect...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Login to view more

Abstract

The invention discloses a manufacturing method and application of quantitative paralytic shellfish toxin detection test paper. Firstly, a carrier pad is arranged at the bottommost layer; a nitrocellulose membrane is paved on the carrier pad; a sample combination pad and a water absorption pad are respectively arranged at two ends of the nitrocellulose membrane; one end of the sample combination pad and one end of the water absorption pad are partially overlapped with the nitrocellulose membrane, an adhesive tape layer covers one end of the sample combination pad, a detection line on the nitrocellulose membrane is close to one end of the sample combination pad, and a quality control line is close to one end of the water absorption pad, wherein the detection line comprises a T1 line and a T2line which are respectively formed by coating an amino terminal with an artificial antigen and coating a guanidyl terminal with an artificial antigen, and the quality control line is formed by a goat-anti-mouse secondary antibody; the sample combination pad is sprayed with a guanidyl-end artificial antigen preparation monoclonal antibody marked with colloidal gold and an amino-end artificial antigen preparation monoclonal antibody marked with colloidal gold. The test paper has the characteristics of good specificity, high sensitivity and simple operation technology, accords with the industrialization development trend, and has the potential of wide popularization.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a production method and application of a quantitative paralytic shellfish toxin detection test paper. Background technique [0002] Paralytic shellfish poisoning (PSP) is the most widely distributed and most harmful type of marine biotoxin in the world. It is mainly produced by unicellular dinoflagellates in the ocean. It has been confirmed that a variety of Alexandrium, chain-like Gymnodinium catenatum and Pyrodinium bahamense can produce paralytic shellfish poisoning. After shellfish filter-feed the toxic algae, the algae toxins accumulate in their bodies, and then pass through the food chain to endanger human health. PSP is relatively stable under weak acid and low temperature conditions. Common cooking methods cannot change its structure and reduce its toxicity. It mainly contaminates filter-feeding shellfish. After the polluted shellfish is eaten by humans, it will produc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/58G01N33/577G01N33/558G01N33/532G01N33/531G01N33/53
CPCG01N33/587G01N33/577G01N33/558G01N33/5308G01N33/532G01N33/531Y02A50/30
Inventor 苏捷许旻乔琨刘智禹
Owner FISHERIES RES INST OF FUJIAN
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products