Manufacturing method and application of quantitative paralytic shellfish toxin detection test paper

Abstract

The invention discloses a manufacturing method and application of quantitative paralytic shellfish toxin detection test paper. Firstly, a carrier pad is arranged at the bottommost layer; a nitrocellulose membrane is paved on the carrier pad; a sample combination pad and a water absorption pad are respectively arranged at two ends of the nitrocellulose membrane; one end of the sample combination pad and one end of the water absorption pad are partially overlapped with the nitrocellulose membrane, an adhesive tape layer covers one end of the sample combination pad, a detection line on the nitrocellulose membrane is close to one end of the sample combination pad, and a quality control line is close to one end of the water absorption pad, wherein the detection line comprises a T1 line and a T2line which are respectively formed by coating an amino terminal with an artificial antigen and coating a guanidyl terminal with an artificial antigen, and the quality control line is formed by a goat-anti-mouse secondary antibody; the sample combination pad is sprayed with a guanidyl-end artificial antigen preparation monoclonal antibody marked with colloidal gold and an amino-end artificial antigen preparation monoclonal antibody marked with colloidal gold. The test paper has the characteristics of good specificity, high sensitivity and simple operation technology, accords with the industrialization development trend, and has the potential of wide popularization.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a production method and application of a quantitative paralytic shellfish toxin detection test paper. Background technique [0002] Paralytic shellfish poisoning (PSP) is the most widely distributed and most harmful type of marine biotoxin in the world. It is mainly produced by unicellular dinoflagellates in the ocean. It has been confirmed that a variety of Alexandrium, chain-like Gymnodinium catenatum and Pyrodinium bahamense can produce paralytic shellfish poisoning. After shellfish filter-feed the toxic algae, the algae toxins accumulate in their bodies, and then pass through the food chain to endanger human health. PSP is relatively stable under weak acid and low temperature conditions. Common cooking methods cannot change its structure and reduce its toxicity. It mainly contaminates filter-feeding shellfish. After the polluted shellfish is eaten by humans, it will produc...

AI Technical Summary

Problems solved by technology

At present, the commonly used shellfish poisoning monitoring methods at home and abroad are mainly the biological mouse method and high performance liquid chromatography (HPLC), but the biological method has the disadvantages of large errors, poor reproducibility, and the inability to analyze specific components in the sample. ; while the HPLC method can accurately determine the type and content of the toxin, but it requires cumbersome pretreatment of the sample and the purchase of expensive equipment

Therefore, the monitoring of paralytic shellfish poisoning in marine biological samples is severely limited

Images