11 results about "Paralytic shellfish toxin" patented technology

The present application belongs to the technical field of toxin detection, and particularly relates to a method for detecting shellfish toxin based on transition metal oxide / gold particle composite SERS substrate; paralytic shellfish poison, i.e. saxitoxin, in the shellfish toxin is selected as a detection object; the specific steps are as follows: firstly, a zinc oxide-silver composite nanoparticle SERS substrate is prepared to enhance the Raman signal of saxitoxin; then, SERS spectra of saxitoxin with different concentrations are collected; the collected spectra are introduced into matlab to establish a prediction model by using a PLS algorithm; an actual sample is treated according to a national standard method to obtain a treatment liquid; then, the SERS spectrum of the treatment liquid is collected and introduced into the prediction model to obtain a prediction result; the present application has the advantages of simple synthesis steps, good SERS enhancement effect, fast detection speed, high stability, and good application prospect in the technical field of harmful factor detection in food and the like.

The present invention discloses a preparation method and application of 13 kinds of paralytic shellfish toxin matrix standard material raw materials, including the following steps: (1) a method for expanding the cultivation of four paralytic shellfish toxin-producing algae, and (2) a step of exposing the four paralytic shellfish toxin-producing algae to mussels. The present invention adopts a method of exposing mussels to a mixture of multiple toxin-producing algae in different proportions to obtain raw materials containing 13 kinds of paralytic shellfish toxin matrix standard materials. By comparing the field exposure method and the indoor combined exposure mussel method of the present invention, the present invention can obtain stable, reliable and repeatable raw materials of a large variety of paralytic shellfish toxin matrix standard materials, which can simultaneously meet the quality control and method verification needs of shellfish paralytic shellfish toxin detection and analysis in different sea areas in China, and promote the effectiveness of monitoring and risk assessment of paralytic shellfish toxins in my country.

The present disclosure is directed, in part, to saxiphilin proteins, nucleic acids encoding the same, compositions comprising the same, kits comprising the same, and methods of detecting toxin in samples. In some embodiments, the toxin is saxitoxin or derivatives of the same.

This invention discloses a method and apparatus for mineralizing paralyzing shellfish poisoning by hydroxyl radicals, relating to the field of marine ecological disaster prevention and control. The method involves: oxygen entering an oxygen plasma generator, being ionized and dissociated to generate oxygen-active particle gas, which is injected into a gas-liquid mixer to efficiently generate a •OH solution, and then injected into a liquid-liquid mixer where it is broken down into a large number of tiny droplets; eight sampling ports are evenly spaced after the liquid-liquid mixer to measure the TRO gradient after mixing and dilution; the large number of •OH droplets rapidly mineralize the paralyzing shellfish poisoning while simultaneously killing the toxin-producing dinoflagellates; detection is performed at the eight sampling ports to establish efficacy-time models for •OH mineralization of shellfish poisoning and •OH mineralization of algae, and to determine the threshold values; the acute and chronic toxicity of the shellfish poisoning control group C2 and the treatment group C3 are evaluated, as are the acute and chronic toxicity of the dinoflagellate control group D2 and the treatment group D3; this invention provides a complete solution from source control to end-of-life assessment.

This invention relates to the field of salicornin detection technology, and particularly to a dual-color visualization analysis and detection method for salicornin. The method includes: taking a shellfish sample to be tested, adding hydrochloric acid solution, boiling and stirring, and centrifuging at low temperature; collecting the supernatant and adjusting the pH of the supernatant to <4 with hydrochloric acid solution; adding PBS solution and mixing well; adding the test solution to a suspension of MNP@Ag-Apt-cDNA-Au / Pt signal probe and incubating at room temperature; magnetic separation, taking the supernatant and adding it to 4-7 times its volume of dual-color development solution, and incubating for 10-20 min; the dual-color development solution includes 1 mM rhodamine 6G, 0.6 mM TMB, and 1 mM hydrogen peroxide in a volume ratio of 0.3-1:10:10; taking images with a camera, extracting the RGB histogram data, analyzing the data, and determining the concentration of salicornin. The advantages are low detection limit and high sensitivity.

The present invention discloses a method and application for detecting saxitoxin, and relates to the field of compound detection technology. The method comprises detecting a sample using ultra-high performance liquid chromatography-tandem mass spectrometry, treating the sample using proton-assisted ionization technology after liquid chromatography detection, and then performing mass spectrometry detection. By using ultra-high performance liquid chromatography-tandem mass spectrometry detection in combination with proton-assisted ionization technology, the detection sensitivity of saxitoxin is improved, and even if the concentration level of saxitoxin in the sample is extremely low, it can be detected, thereby ensuring the safety of aquatic products.

The present application relates to a kind of anti-saxitoxin recombinant rabbit monoclonal antibody and its application, the amino acid sequence of the anti-saxitoxin recombinant rabbit monoclonal antibody includes heavy chain variable region and light chain variable region, respectively as shown in SEQ ID NO:1 And 10 shown in formula.The high sensitivity of it, the lowest saxitoxin can reach 7.3 pg mL ‑1 IC 50 , linear range is 0.0019 ng L ‑1 0.0282 ng L ‑1 ;Meanwhile, it also has high specificity, and other paralytic shellfish toxins and pufferfish toxin are not cross-reacted.The anti-saxitoxin recombinant rabbit monoclonal antibody prepared in the present application, compared with the anti-saxitoxin mouse monoclonal antibody on sale, the antibody provided in the present application is high in detection sensitivity, high in specificity, good in affinity, high in accuracy;It is suitable for immunological analysis kit, immunological test strip, immunoblotting and other clinical and scientific research applications.

The invention discloses application of an aptamer electrochemical sensing electrode based on an MXene-PPy composite conducting layer to saxitoxin detection, the electrochemical aptamer sensing electrode MCH / T5 / M-30f / AuNPs / MXene-PPy / SPCE is researched and developed, the MXene-PPy composite conducting layer solves the problems of self-stacking and oxidative inactivation of MXene, and the conductivity, electrochemical activity and long-term stability of the electrode are improved; the SPCE substrate is adopted, so that the cost is low, and the portability is high; by combining aptamer specific recognition and composite conductive layer signal amplification characteristics, trace detection of STX is realized, the detection limit is 0.063 nmol / L, the quantitation limit is 0.1 nmol / L, and the detection limit is smaller than the limit requirement of STX in shellfish, namely 80mu g / 100g (0.2673 nmol / L).

The application belongs to the field of analysis and detection, and particularly relates to preparation of a bifunctional imprinting fluorescent probe and sensitive detection of marine biological toxins and antiviral drugs. The application adopts valganciclovir hydrochloride (VAL) similar in structure to saxitoxin (STX) as a pseudo-template, avoids the high toxicity of saxitoxin (STX) and the risk of template leakage, adopts a primary-secondary functional monomer synergistic strategy to construct a high-specificity recognition site, combines a fluorescent dye occupation-competition release mechanism to realize fluorescence signal transduction, and prepares an imprinting polymer fluorescent probe which has high selectivity, high sensitivity and fast response speed for STX and VAL, and can simultaneously adapt to the detection requirements of marine environmental samples and clinical biological samples, and still maintains excellent recovery rate and stability in a complex matrix.