51 results about "Paralytic shellfish toxin" patented technology

The invention relates to biological technical field, and provides a high affinity adapter body capable of specifically binding with saxitoxin acetate, and the sequence of single-stranded DNA adapter body as SEQ ID NO: 2. By mutation and truncation of an in vitro screened adapter body, a high affinity single-stranded oligonucleotide adapter body capable of specifically identifying the saxitoxin acetate can be obtained. The adapter body has a broad application prospect, and can be used for separation and enrichment of trace concentrations of STX (saxitoxin) in a sample, rapid detection of saxitoxin, and preparation of a drug for neutralization of saxitoxin sodium ion channel inhibition effect, and removal of toxins in water.

The invention discloses a high performance liquid chromatography mass spectrometry detecting method of 16 fat soluble saxitoxins in shellfish meet. The detecting method comprises the following steps of: processing a sample, purifying, testing the condition, qualitatively testing and quantitatively testing. According to the detecting method, methanol is adopted for extraction and a solid phase extraction column is adopted for purification so as to carry out the synchronous detection on the 16 fat soluble saxitoxins in the shellfish meet. The method is simple and convenient to operate, good in a purification effect, high in overall recycling rate and strong in reconstruction capability, and meanwhile an organic solvent and the processing time are saved, and the pretreatment process of the sample is simplified.

The invention relates to a detection device for detecting red tide virus. An immune colloidal gold test strip for rapidly detecting paralytic shellfish poison disclosed herein comprises a sample pad, a gold jed pad, a Sartorius NC film, an absorbent pad and a PVC backing, wherein, the sample pad, the gold jed pad, the Sartorius NC film, and the absorbent pad are attached to the PVC backing orderly, the gold jed pad is coated with a colloidal gold-labeled paralytic shellfish poison monoclonal antibody, and the Sartorius NC film is respectively coated with a detection line consisting of Saxitoxin-bovine serum albumin conjugate and a quality control line consisting of goat-anti-mouse antibodies. The test strip has the advantages of strong specificity, high detection sensitivity, rapid detection, simple pre-treatment, no need of any device, convenience in carrying, low cost of the detection, simple operation, no need of professional personnel to operate, convenient storage, good stability, and high accuracy and high precision of the detection result, and the test strip can be stored for at least 6 months at room temperature.

The invention provides a set of single stranded DNA (ssDNA) aptamers DA-01 and TTX-27 which can simultaneously recognize domoic acid (DA) and tetrodotoxin (TTX), including one ssDNA aptamer DA-06 which specifically recognizes DA, one ssDNA aptamer TTX-07 which can specifically recognize TTX, and one ssDNA aptamer STX-41 which can specifically recognize saxitoxin (STX). According to the invention,magnetic reduced graphene oxide (MRGO) is adopted to assist in separated systematic evolution of ligands by exponential enrichment (SELEX), properties of MRGO of being capable of adsorbing free ssDNA,but not adsorbing ssDNA combined with target molecules are utilized, and magnetic separation enrichment characteristics of MRGO are further used to separate ssDNA with affinity and without affinity to target toxins; primary structure homology and secondary structure similarity on obtained ssDNA are analyzed after 16 repeated rounds of incubation, separation, amplification and in-vitro screening for single-strand preparation, then the affinity and the specificity are measured, and finally one set of the aptamers with high affinity and specificity is obtained. The set of the aptamers has broadapplication prospects in the aspects of analysis, detection, separation, enrichment, removal and purification for DA, TTX and STX in aquatic products.

The invention provides a removal method of paralytic shellfish poison, comprising the following steps: firstly, absorbent is distributed in embedding medium evenly; secondly, the embedding medium which contains the absorbent is solidified to form gel; thirdly, the solidified embedding medium which contains the absorbent is made into gel balls or blocks with uniform size; fourthly, the balls or the blocks are put in the solution which contains the paralytic shellfish poison, and the solution is then stirred for 5-30 minutes; and lastly, the gel is removed by filtration or centrifuge method to get the water which has removed the paralytic shellfish poison. The invention has the advantages that the absorbent which is treated by embedding can absorb the paralytic shellfish poison in the water. The absorption efficiency is higher than both pure absorbent and embedding medium. The embedded absorbent is comparatively big grain which is convenient to be separated from the system. The method can be used to remove the paralytic shellfish poison in the water, can be used for the enrichment and detection of positions, the purification of shellfish and so on, and can be used for the purification of other positions and pollutants in the environment after being improved.

The invention relates to a saxitoxin artificial antigen, an anti-saxitoxin antibody prepared by the saxitoxin artificial antigen, and their preparation methods and application. Through carbodiimide method, amino groups in saxitoxin (STX) are connected with carboxyl groups in ovalbumin (OVA) as a carrier protein. A high purity saxitoxin immunization antigen STX-OVA is obtained through dialysis utilizing a gradient method. The prepared high purity saxitoxin artificial immunization antigen STX-OVA is utilized for preparation of a polyclonal antibody and the polyclonal antibody is utilized for preparation of an enzyme-linked immunosorbent assay kit utilized for detecting saxitoxin. In the invention, saxitoxin artificial antigens of STX-OVA and STX-BSA are synthesized successfully to immunize BALB/c mice and then a polyclonal antibody with high titer and strong singularity is obtained. An enzyme-linked immunosorbent assay kit prepared by an established enzyme-linked immunoassay can be utilized for the rapid detection of saxitoxin.

The invention discloses a preparation method of a saxitoxin molecularly-imprinted nano fluorescent material and an application. The preparation method comprises the steps of taking saxitoxin as a template molecule and adding a quantum dot fluorescent nanomaterial; and initializing polymerization in the presence of a cross-linking agent tetraethylortho silicate and a functional monomer 3-aminopropyltriethoxysilane or 3-(tri(trimethsilyl)silyl)propyl methacrylate, and then removing the template molecule in the obtained polymer by adopting an ultrasonic-assisted extraction method to obtain the saxitoxin molecularly-imprinted nano fluorescent material capable of specifically identifying the saxitoxin. The saxitoxin molecularly-imprinted nano fluorescent material can be used for detecting the content of the saxitoxin in a shell sample, and has the advantages of uniform particle sizes, good selectivity and excellent fluorescent stability; and fast and high-sensitivity detection of the saxitoxin in the shell sample can be achieved.

The invention discloses an automatic pretreatment device for processing paralytic shellfish toxic samples. The device comprises a controller, a motor with a large torque force, a cutter head, a grinding cavity, a first suction connecting pipe, a filter upper cover, a filter paper bag, a filter paper sheet, a filter lower cover, a second suction connecting pipe, a suction filter, and a collecting bottle. The cutter is arranged on the large torque force motor and forms an enclosed space with the grinding cavity. The grinding cavity is connected to the filter upper cover through the first suction connecting pipe. The filter upper cover and the filer lower cover are connected to form an enclosed filter. In the filter, the filter paper bag and the filter paper sheet are arranged in sequence from top to bottom, the filter lower cover is connected to the top end of the second suction connecting pipe in the filter, and the controller is individually connected to the grinding cavity, the filter, and the suction filter though air pipes or liquid pipes. The provided device can automatically process shellfish meat samples before experiments for detecting the paralytic shellfish toxin, the pretreatment operation is simplified, and the pretreatment efficiency is improved.

The invention discloses a method for detecting paralytic shellfish poisoning. The method is implemented on a mouse neuroma cell impedance sensor. The method comprises the following steps: inoculating neuro2a cells to sensor chip cell culture holes for culturing; respectively adding a to-be-detected solution into cell culture holes a solution containing ouabain and veratridine; and detecting a CI value of each cell culture hole after the to-be-detected solution is added, and judging the content of the paralytic shellfish poisoning in the to-be-detected solution according to change of the CI value. According to the method, cells are directly taken as detection carriers, the operation is simple, and the cost is low. According to the method, the detection limit of saxitoxin is low to 0.9ng/ml and has obvious progress compared with the detection limit (160ng/ml) of mouse bioassay in a current national standard detection method. In addition, the method is slightly influenced by interference effect of shellfish meat matrixes and reliable in data.

A simple solid phase extraction (SPE) method for continuous sequestering and concentration of waterborne cues from sea water conditioned with aquatic source organisms that induce toxin formation in dinoflagellates, and a method of increasing this toxin formation.

The invention relates to a method for quickly screening and identifying various paralytic shellfish toxins in red tide algae. The method comprises the following steps: weighing a certain quantity of red tide algae samples, putting into a centrifugal tube, adding a certain quantity of extracting solvents, crushing by adopting a cell crusher or an algae grinding method, then extracting the various paralytic shellfish toxins and combining into an extracting solution; performing centrifugal separation to remove a precipitate, absorbing supernatant liquid, filtering through a micro-porous filtering membrane and injecting into a sample bottle for later use; performing separation analysis on the paralytic shellfish toxins in the red tide algae crude extracting liquid by adopting a high-performance liquid chromatography-high resolution mass spectrometry; through comparing information related to the accurate molecular weight of the common paralytic shellfish toxins in the red tide algae, the quick identification on the various common paralytic shellfish toxins in the red tide algae is realized. The method provided by the invention can be used in a department of marine environmental management to quickly determine whether the paralytic shellfish toxins are contained in the red tide algae or not and is further used for judging the influence of the marine shellfish toxins on aquatic products to ensure the consumption safety of the aquatic products.

The invention discloses malic acid-chitosan nanopore hydrogel microspheres as well as a preparation method and application thereof, and belongs to the technical field of guaranteeing the safety of aquatic food and preparing paralytic shellfish toxin bio-adsorbents. The invention discloses a preparation method of malic acid-chitosan nanopore hydrogel microspheres. The preparation method specifically comprises the following steps: preparing malic acid and chitosan into hydrogel; preparing a malic acid-chitosan nano-pore hydrogel microsphere, adding nano-silica and glycerin into the prepared hydrogel, adding sodium hydroxide, completely dissolving the nano-silica under an alkaline condition to form uniformly distributed nano-pores, performing washing, performing freeze-drying, and performing grinding and sieving to obtain the malic acid-chitosan nano-pore hydrogel microsphere. The malic acid-chitosan nanopore hydrogel microspheres prepared by the invention can be used as an efficient adsorbent for adsorbing and removing paralytic saxitoxin in a water body. The preparation method disclosed by the invention is simple, convenient to use and easy to store after being dried, and has great application significance on shellfish toxin pollution and improvement of product safety.

The invention provides a modifier capable of reducing saxitoxin. The modifier capable of reducing saxitoxin is characterized by comprising the following components in parts by weight: 10-100 parts of garlicin, 10-200 parts of astaxanthin, 10-200 parts of beta-cyclodextrin, 10-200 parts of quercetin, 500-1000 parts of ethanol and 500-1000 parts of water. The modifier is capable of filling up the blank in the industry; the modifier which utilizes garlicin and astaxanthin as main components is capable of reducing the saxitoxin in food by no less than 50%, and does not cause substitutability influence on original flavor of the food; furthermore, the modifier adopts natural components and conforms to the food safety requirement.

Provided herein are compounds, pharmaceutical compositions comprising the compounds, and methods of using the compounds and compositions in treating conditions associated with voltage-gated sodium channel function, for example conditions associated with pain. The compounds are 10′,11′-modified saxitoxins. The compounds are optionally additionally modified at carbon 13. In certain embodiments, the 10′,11′-modified saxitoxins are of Formula I: where R¹, R² and R³ are as described herein. Also provided herein are methods of treating pain in a mammal comprising administering an effective treatment amount of a 10′,11′ modified saxitoxin or composition to a mammal. In an embodiment, the mammal is a human.